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The map label for this gene is citZ [H]

Identifier: 13473292

GI number: 13473292

Start: 3081372

End: 3082511

Strand: Direct

Name: citZ [H]

Synonym: mlr3843

Alternate gene names: 13473292

Gene position: 3081372-3082511 (Clockwise)

Preceding gene: 13473289

Following gene: 13473294

Centisome position: 43.79

GC content: 70.18

Gene sequence:

>1140_bases
ATGTCGGAATGGTTATCGCGGGAAGAGGCGCTGGAAAGGCTTAGGGTGCGGCCGCAGACGCTCTATGCCTATGTCAGCCG
CGGGCGCATCGGCATGCGCCCCGACCGGGCCGATCCGCGCCGCAGCCAGTATCGCGCCGACGACATCGCGGCGCTGGCAA
CACGCCGGGCGCGCGGACGCAGCCCCTCGGCCATCGCCGAAAGCGCGATTGCCTGGGGCGAGCCGTCGATCGCAACGGCG
ATTTCGACCGTCTGGCACGGTCACCTCATCTATCGGGGCCGGGACGCCGCCGTGCTGTCCGACAATGCCACGCTGGAAGA
GACCGCCGCCGTGCTTTGGGGCCTGCCTGAACCGATCCGCTTCGAGGCGCCGGCGCCGGATGCACCGCGCCAGCCCGGCC
GAGCCTCGGCCTTTGCGCAACTTGCCTTGCTCGCCGGCCAGGCTCGGCCTTCGCTAGGACGCAGCGCCGCTTCGTTATGT
GCCGACGCCGCGCGCGCAATCGGTGTGCTGGCCGGCGCCCTCGGTGCCGAAGCGGGGCCGGATCCGGTGCATCGGCGATT
GGCGCGGGGCTGGTCAGTGGATGATGCCGGTGCGGATGCGATCCGGCGCGCCCTTGTCCTGCTTGCCGATCATGAACTCA
ACGCATCGACCTTCGCTGCCCGCGTGGCGGCCTCGACCGGGGCATCGCCTGCCGCATGCCTGCTGGCGGGCCTGGCAACG
CTATCCGGCCCGCGCCATGGCGGCGCCGGCGAGGCAGTGATGCAACTCGCCGAGGATGCCGCAAGACACGGCGCCGATGC
GGCGATCCGGCGCTGGCTCGGCCACGACCGGCCGCTGCCGGCATTCGGTCATCCGCTTTATCCGGACGGGGATCCCCGTA
CCACCGCACTTTCGGCAGCTATCCCTGTCGACGAGACGCTGCAGCAGTTGGAAAAGGCCGCCATTGCCATGACCGGCGCG
CGCCCGAACATCGACTTCGCCCTGGCTGCGCTGACGCGCGGTCTTGGCTTGCCGCGCGATGCGCCGTTCCAGCTGTTCGC
GCTCGGGCGCAGTGTTGGCTGGGCCGCCCATATGGTCGAGCAGATCGTCAGCGGCAGCATCATCCGGCCGCGCGGCCGCT
ATGAAGGGCTGTTGCCCTGA

Upstream 100 bases:

>100_bases
TCGCCATAGCCTTGTCTCCTTGAAGCATGCCCGGATCGGAGATAAGGCTGATCAACCTAAGCATCAATCTTGATCAATAG
AATCAATATCAGAGCCCAAA

Downstream 100 bases:

>100_bases
CGGCGTGCGAAGTCGTCAGGCGGAGTTGGCATTGGTCCCGAACCGCGCCCGGTACGCGGCGGGCGTCGTGGCGAATTGCC
GGCGGAAATGATGCCGCAAC

Product: citrate synthase II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MSEWLSREEALERLRVRPQTLYAYVSRGRIGMRPDRADPRRSQYRADDIAALATRRARGRSPSAIAESAIAWGEPSIATA
ISTVWHGHLIYRGRDAAVLSDNATLEETAAVLWGLPEPIRFEAPAPDAPRQPGRASAFAQLALLAGQARPSLGRSAASLC
ADAARAIGVLAGALGAEAGPDPVHRRLARGWSVDDAGADAIRRALVLLADHELNASTFAARVAASTGASPAACLLAGLAT
LSGPRHGGAGEAVMQLAEDAARHGADAAIRRWLGHDRPLPAFGHPLYPDGDPRTTALSAAIPVDETLQQLEKAAIAMTGA
RPNIDFALAALTRGLGLPRDAPFQLFALGRSVGWAAHMVEQIVSGSIIRPRGRYEGLLP

Sequences:

>Translated_379_residues
MSEWLSREEALERLRVRPQTLYAYVSRGRIGMRPDRADPRRSQYRADDIAALATRRARGRSPSAIAESAIAWGEPSIATA
ISTVWHGHLIYRGRDAAVLSDNATLEETAAVLWGLPEPIRFEAPAPDAPRQPGRASAFAQLALLAGQARPSLGRSAASLC
ADAARAIGVLAGALGAEAGPDPVHRRLARGWSVDDAGADAIRRALVLLADHELNASTFAARVAASTGASPAACLLAGLAT
LSGPRHGGAGEAVMQLAEDAARHGADAAIRRWLGHDRPLPAFGHPLYPDGDPRTTALSAAIPVDETLQQLEKAAIAMTGA
RPNIDFALAALTRGLGLPRDAPFQLFALGRSVGWAAHMVEQIVSGSIIRPRGRYEGLLP
>Mature_378_residues
SEWLSREEALERLRVRPQTLYAYVSRGRIGMRPDRADPRRSQYRADDIAALATRRARGRSPSAIAESAIAWGEPSIATAI
STVWHGHLIYRGRDAAVLSDNATLEETAAVLWGLPEPIRFEAPAPDAPRQPGRASAFAQLALLAGQARPSLGRSAASLCA
DAARAIGVLAGALGAEAGPDPVHRRLARGWSVDDAGADAIRRALVLLADHELNASTFAARVAASTGASPAACLLAGLATL
SGPRHGGAGEAVMQLAEDAARHGADAAIRRWLGHDRPLPAFGHPLYPDGDPRTTALSAAIPVDETLQQLEKAAIAMTGAR
PNIDFALAALTRGLGLPRDAPFQLFALGRSVGWAAHMVEQIVSGSIIRPRGRYEGLLP

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786939, Length=199, Percent_Identity=36.6834170854271, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1786527, Length=188, Percent_Identity=32.4468085106383, Blast_Score=109, Evalue=3e-25,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011278
- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 39984; Mature: 39853

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEWLSREEALERLRVRPQTLYAYVSRGRIGMRPDRADPRRSQYRADDIAALATRRARGR
CCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
SPSAIAESAIAWGEPSIATAISTVWHGHLIYRGRDAAVLSDNATLEETAAVLWGLPEPIR
CCHHHHHHHHHCCCCHHHHHHHHHHHCEEEEECCCCEEECCCCCHHHHHHHHHCCCCCCE
FEAPAPDAPRQPGRASAFAQLALLAGQARPSLGRSAASLCADAARAIGVLAGALGAEAGP
ECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
DPVHRRLARGWSVDDAGADAIRRALVLLADHELNASTFAARVAASTGASPAACLLAGLAT
CHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHH
LSGPRHGGAGEAVMQLAEDAARHGADAAIRRWLGHDRPLPAFGHPLYPDGDPRTTALSAA
HCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHEEEC
IPVDETLQQLEKAAIAMTGARPNIDFALAALTRGLGLPRDAPFQLFALGRSVGWAAHMVE
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
QIVSGSIIRPRGRYEGLLP
HHHCCCEECCCCCCCCCCC
>Mature Secondary Structure 
SEWLSREEALERLRVRPQTLYAYVSRGRIGMRPDRADPRRSQYRADDIAALATRRARGR
CHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
SPSAIAESAIAWGEPSIATAISTVWHGHLIYRGRDAAVLSDNATLEETAAVLWGLPEPIR
CCHHHHHHHHHCCCCHHHHHHHHHHHCEEEEECCCCEEECCCCCHHHHHHHHHCCCCCCE
FEAPAPDAPRQPGRASAFAQLALLAGQARPSLGRSAASLCADAARAIGVLAGALGAEAGP
ECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
DPVHRRLARGWSVDDAGADAIRRALVLLADHELNASTFAARVAASTGASPAACLLAGLAT
CHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHH
LSGPRHGGAGEAVMQLAEDAARHGADAAIRRWLGHDRPLPAFGHPLYPDGDPRTTALSAA
HCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHEEEC
IPVDETLQQLEKAAIAMTGARPNIDFALAALTRGLGLPRDAPFQLFALGRSVGWAAHMVE
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
QIVSGSIIRPRGRYEGLLP
HHHCCCEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9006016; 12397186 [H]