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The map label for this gene is pyk [H]

Identifier: 13473275

GI number: 13473275

Start: 3062565

End: 3064001

Strand: Reverse

Name: pyk [H]

Synonym: mll3819

Alternate gene names: 13473275

Gene position: 3064001-3062565 (Counterclockwise)

Preceding gene: 13473276

Following gene: 13473270

Centisome position: 43.55

GC content: 66.18

Gene sequence:

>1437_bases
ATGAGACGCAGCCGCAAGGTCAAGATCCTAGCCACAATCGGTCCGGCCTCCTCCTCCGAGGAGATGCTGCAGAAGCTGTT
CGAAGCGGGCGCCGATGTGTTCCGCATCAACATGAGCCATACCGACCACGAACTGATGCGCACGCTGGTCGGGCGCATCC
GCGCCGTCGAGGAAAAGGTCGGCCGGCCGATCGGCATCCTCGCCGACCTGCAAGGCCCCAAGCTGCGCGTCGGCAAGTTC
GCCAATGGCAAGGAGGTGCTGACGCCAGGCCAGACCTTCACGCTCGACGACAATCCCGAGCCCGGCACGTCGAGCAGGGT
CTATCTGCCGCATCCGGAAATCCTGAGTTCGGTCGAGCCCGGTCACCGTCTTTTGATCGACGACGGCAAGCTCGAGCTGA
AGGCGGTGAAGAGCGATGGCAAGTCGATCGTCTGCACGGTCGTCGCCGGCACCACGATTTCCGACAAGAAGGGCGTCAGC
CTGCCCGATACCGACCTGCCGGTCGGCGCTCTGACCGAAAAGGACCGCAAGGATCTCGACGCGGTGCTCGCCACGGGCGT
CGACTGGATCGCGCTGTCCTTCGTGCAGCGCCCCGAGGATCTGGCCGAGGCGCGCAAGATCGCGCGGGGCCGGGCGCTGA
TCATGGCCAAGATCGAAAAGCCGCAGGCCGTCGCCCGCTTGGCCGAGATCATCGAACTGTCCGACGCGCTGATGGTCGCC
CGTGGCGATCTCGGCGTCGAAATGCCGCTGGAAGCCGTTCCCGGCATCCAGAAGCAGATCACGCGCGCCGCGCGCCGCGC
CGGCAAGCCGGTGGTGGTCGCCACGCAAATGCTGGAATCGATGATCACCGCCCCGGTGCCGACCCGCGCCGAAGTCTCCG
ACGTCTCGATCGCCGTCTTCGAGGGCGCCGACGCCATCATGCTGTCGGCGGAATCGGCGGCGGGTGCCTATCCCGTCGAA
GCCGTGGCGATGATGGACCGCATCGCCACCAAGGTCGAAACCGATCCGACCTATGCCGGCATCATCAACGCCCAGCGCTC
CGAGCCGGAAGCGACCGGAGCCGACGCGATCTCGCTGGCTGCCCGCGAAATCGCCGAGACTCTGAAACTGTCGGCGATCA
TCACCTACACGGCGTCCGGCACCACCGGGCTGCGCGCCGCGCGCGAACGGCCACAGGTGCCGATCATCGCTCTGTCGCCG
ATCCTCAACACCGCACGGCGGCTGTCGCTTCTGTGGGGCACGCACTGTGTCGTTTCGCCCGATGCCACCGATCTCGACGA
CATGGTCAACCGCGCCTGCCGCATTGCGCTGGAAGAAGAGTTCGGCAAGCCGGGCGACCGCGTCATCATCACGGCCGGTG
TGCCGCTGAGGACGCCCGGCTCGACCAACATGCTGCGCATTGCCTATGTCGGGTCGGAAAAGCAGGGCAGCCGGTAA

Upstream 100 bases:

>100_bases
TTCAGGAATATGACACGGGCGAACAGGCAAGCTGGATGGTCCAGCTGACCGATGAGCCCGCAACGGGAGGCGCACCAGCC
GCCCCGGGAACAAATAGTCA

Downstream 100 bases:

>100_bases
GCAGCTGATTTCGTGACTGAAGAGGCGTGTCGCGCGGATTCGTGCGGTTCGCCGTGGCTGGAATACAAAGGCGCGGGGCA
ATGGTCCCAGCCTTCATCTA

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MRRSRKVKILATIGPASSSEEMLQKLFEAGADVFRINMSHTDHELMRTLVGRIRAVEEKVGRPIGILADLQGPKLRVGKF
ANGKEVLTPGQTFTLDDNPEPGTSSRVYLPHPEILSSVEPGHRLLIDDGKLELKAVKSDGKSIVCTVVAGTTISDKKGVS
LPDTDLPVGALTEKDRKDLDAVLATGVDWIALSFVQRPEDLAEARKIARGRALIMAKIEKPQAVARLAEIIELSDALMVA
RGDLGVEMPLEAVPGIQKQITRAARRAGKPVVVATQMLESMITAPVPTRAEVSDVSIAVFEGADAIMLSAESAAGAYPVE
AVAMMDRIATKVETDPTYAGIINAQRSEPEATGADAISLAAREIAETLKLSAIITYTASGTTGLRAARERPQVPIIALSP
ILNTARRLSLLWGTHCVVSPDATDLDDMVNRACRIALEEEFGKPGDRVIITAGVPLRTPGSTNMLRIAYVGSEKQGSR

Sequences:

>Translated_478_residues
MRRSRKVKILATIGPASSSEEMLQKLFEAGADVFRINMSHTDHELMRTLVGRIRAVEEKVGRPIGILADLQGPKLRVGKF
ANGKEVLTPGQTFTLDDNPEPGTSSRVYLPHPEILSSVEPGHRLLIDDGKLELKAVKSDGKSIVCTVVAGTTISDKKGVS
LPDTDLPVGALTEKDRKDLDAVLATGVDWIALSFVQRPEDLAEARKIARGRALIMAKIEKPQAVARLAEIIELSDALMVA
RGDLGVEMPLEAVPGIQKQITRAARRAGKPVVVATQMLESMITAPVPTRAEVSDVSIAVFEGADAIMLSAESAAGAYPVE
AVAMMDRIATKVETDPTYAGIINAQRSEPEATGADAISLAAREIAETLKLSAIITYTASGTTGLRAARERPQVPIIALSP
ILNTARRLSLLWGTHCVVSPDATDLDDMVNRACRIALEEEFGKPGDRVIITAGVPLRTPGSTNMLRIAYVGSEKQGSR
>Mature_478_residues
MRRSRKVKILATIGPASSSEEMLQKLFEAGADVFRINMSHTDHELMRTLVGRIRAVEEKVGRPIGILADLQGPKLRVGKF
ANGKEVLTPGQTFTLDDNPEPGTSSRVYLPHPEILSSVEPGHRLLIDDGKLELKAVKSDGKSIVCTVVAGTTISDKKGVS
LPDTDLPVGALTEKDRKDLDAVLATGVDWIALSFVQRPEDLAEARKIARGRALIMAKIEKPQAVARLAEIIELSDALMVA
RGDLGVEMPLEAVPGIQKQITRAARRAGKPVVVATQMLESMITAPVPTRAEVSDVSIAVFEGADAIMLSAESAAGAYPVE
AVAMMDRIATKVETDPTYAGIINAQRSEPEATGADAISLAAREIAETLKLSAIITYTASGTTGLRAARERPQVPIIALSP
ILNTARRLSLLWGTHCVVSPDATDLDDMVNRACRIALEEEFGKPGDRVIITAGVPLRTPGSTNMLRIAYVGSEKQGSR

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286418, Length=489, Percent_Identity=38.0368098159509, Blast_Score=271, Evalue=8e-73,
Organism=Homo sapiens, GI33286422, Length=489, Percent_Identity=37.6278118609407, Blast_Score=271, Evalue=1e-72,
Organism=Homo sapiens, GI33286420, Length=489, Percent_Identity=37.6278118609407, Blast_Score=271, Evalue=1e-72,
Organism=Homo sapiens, GI32967597, Length=490, Percent_Identity=37.3469387755102, Blast_Score=261, Evalue=1e-69,
Organism=Homo sapiens, GI10835121, Length=490, Percent_Identity=37.3469387755102, Blast_Score=260, Evalue=2e-69,
Organism=Homo sapiens, GI310128732, Length=288, Percent_Identity=36.1111111111111, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI310128730, Length=288, Percent_Identity=36.1111111111111, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI310128736, Length=238, Percent_Identity=32.3529411764706, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI310128734, Length=238, Percent_Identity=32.3529411764706, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI310128738, Length=214, Percent_Identity=30.3738317757009, Blast_Score=92, Evalue=9e-19,
Organism=Escherichia coli, GI1788160, Length=479, Percent_Identity=40.2922755741127, Blast_Score=302, Evalue=4e-83,
Organism=Escherichia coli, GI1787965, Length=465, Percent_Identity=36.1290322580645, Blast_Score=275, Evalue=6e-75,
Organism=Caenorhabditis elegans, GI17544584, Length=484, Percent_Identity=37.1900826446281, Blast_Score=278, Evalue=5e-75,
Organism=Caenorhabditis elegans, GI71984413, Length=482, Percent_Identity=36.7219917012448, Blast_Score=259, Evalue=2e-69,
Organism=Caenorhabditis elegans, GI17506829, Length=482, Percent_Identity=36.7219917012448, Blast_Score=259, Evalue=3e-69,
Organism=Caenorhabditis elegans, GI71984406, Length=482, Percent_Identity=36.7219917012448, Blast_Score=258, Evalue=4e-69,
Organism=Caenorhabditis elegans, GI17506831, Length=482, Percent_Identity=36.7219917012448, Blast_Score=258, Evalue=5e-69,
Organism=Saccharomyces cerevisiae, GI6319279, Length=484, Percent_Identity=34.297520661157, Blast_Score=243, Evalue=5e-65,
Organism=Saccharomyces cerevisiae, GI6324923, Length=488, Percent_Identity=33.6065573770492, Blast_Score=238, Evalue=2e-63,
Organism=Drosophila melanogaster, GI24648964, Length=484, Percent_Identity=36.9834710743802, Blast_Score=269, Evalue=4e-72,
Organism=Drosophila melanogaster, GI28571814, Length=484, Percent_Identity=36.9834710743802, Blast_Score=268, Evalue=5e-72,
Organism=Drosophila melanogaster, GI24581235, Length=491, Percent_Identity=31.5682281059063, Blast_Score=234, Evalue=1e-61,
Organism=Drosophila melanogaster, GI24648966, Length=402, Percent_Identity=36.318407960199, Blast_Score=231, Evalue=6e-61,
Organism=Drosophila melanogaster, GI24646914, Length=256, Percent_Identity=39.84375, Blast_Score=166, Evalue=5e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51152; Mature: 51152

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRSRKVKILATIGPASSSEEMLQKLFEAGADVFRINMSHTDHELMRTLVGRIRAVEEKV
CCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHH
GRPIGILADLQGPKLRVGKFANGKEVLTPGQTFTLDDNPEPGTSSRVYLPHPEILSSVEP
CCCEEEEEECCCCCEEECCCCCCCEEECCCCEEEECCCCCCCCCCEEECCCHHHHHCCCC
GHRLLIDDGKLELKAVKSDGKSIVCTVVAGTTISDKKGVSLPDTDLPVGALTEKDRKDLD
CCEEEEECCCEEEEEEECCCCEEEEEEEECCEECCCCCCCCCCCCCCCCCCCCCHHHHHH
AVLATGVDWIALSFVQRPEDLAEARKIARGRALIMAKIEKPQAVARLAEIIELSDALMVA
HHHHCCHHHHHHHHHHCCHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHEEE
RGDLGVEMPLEAVPGIQKQITRAARRAGKPVVVATQMLESMITAPVPTRAEVSDVSIAVF
ECCCCCCCCHHHCCCHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCCCCCCEEEEEE
EGADAIMLSAESAAGAYPVEAVAMMDRIATKVETDPTYAGIINAQRSEPEATGADAISLA
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCHHHHHH
AREIAETLKLSAIITYTASGTTGLRAARERPQVPIIALSPILNTARRLSLLWGTHCVVSP
HHHHHHHHEEEEEEEEECCCCCHHHHHHHCCCCCEEEECHHHHHHHHHHHHHCCEEEECC
DATDLDDMVNRACRIALEEEFGKPGDRVIITAGVPLRTPGSTNMLRIAYVGSEKQGSR
CCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEECCCCCCCEEEEEEECCCCCCCC
>Mature Secondary Structure
MRRSRKVKILATIGPASSSEEMLQKLFEAGADVFRINMSHTDHELMRTLVGRIRAVEEKV
CCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHH
GRPIGILADLQGPKLRVGKFANGKEVLTPGQTFTLDDNPEPGTSSRVYLPHPEILSSVEP
CCCEEEEEECCCCCEEECCCCCCCEEECCCCEEEECCCCCCCCCCEEECCCHHHHHCCCC
GHRLLIDDGKLELKAVKSDGKSIVCTVVAGTTISDKKGVSLPDTDLPVGALTEKDRKDLD
CCEEEEECCCEEEEEEECCCCEEEEEEEECCEECCCCCCCCCCCCCCCCCCCCCHHHHHH
AVLATGVDWIALSFVQRPEDLAEARKIARGRALIMAKIEKPQAVARLAEIIELSDALMVA
HHHHCCHHHHHHHHHHCCHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHEEE
RGDLGVEMPLEAVPGIQKQITRAARRAGKPVVVATQMLESMITAPVPTRAEVSDVSIAVF
ECCCCCCCCHHHCCCHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCCCCCCEEEEEE
EGADAIMLSAESAAGAYPVEAVAMMDRIATKVETDPTYAGIINAQRSEPEATGADAISLA
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCHHHHHH
AREIAETLKLSAIITYTASGTTGLRAARERPQVPIIALSPILNTARRLSLLWGTHCVVSP
HHHHHHHHEEEEEEEEECCCCCHHHHHHHCCCCCEEEECHHHHHHHHHHHHHCCEEEECC
DATDLDDMVNRACRIALEEEFGKPGDRVIITAGVPLRTPGSTNMLRIAYVGSEKQGSR
CCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEECCCCCCCEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA