Click here to switch to the map view.

The map label for this gene is gcvT [H]

Identifier: 13473169

GI number: 13473169

Start: 2969964

End: 2971223

Strand: Direct

Name: gcvT [H]

Synonym: mlr3677

Alternate gene names: 13473169

Gene position: 2969964-2971223 (Clockwise)

Preceding gene: 13473168

Following gene: 13473170

Centisome position: 42.21

GC content: 65.0

Gene sequence:

>1260_bases
ATGGCCCAGGCAACCAGGAAGAAGCCCGTTGCAAAGACGGCCGATGCGGCTGTCTCCGCCCGCATCGCCGCGCAATCGCA
TTTCCGCACGCTGCGCCTCGGCACGCCGTTCCAGCCGCGCCTCGATGCGCTGGCCAAAACGCAGGACTGGTACAATTGGG
CCGGCTACCGTGCGCCACATTCGCTGTGGGACGAGGAACTCGAATATTTCGCCATCCGCAGCCAGGCGGCGCTGTTCGAC
ATCTCGCCGATGACCAAATACCGGATCGAAGGGCCGGATGCCGAAGCCTTCCTCGACCGTGTCACGCTGCGCGATGTCAC
CAGGCTCAGGCCCGGCCGTGTCCATTACACGGCATGGTGTGACGACGAGGGGTTTGTCCTCGACGACGGCACGCTGTTTC
GCCTGTCGCCGACGCGTTTCCGCCTGTGTTCGCAGGAACGGCATCTGCCCTGGCTGCTCGACAGCGCCATTGGTTTCGAT
GTGACAGTCGAAGAAGAGACCGAGGCCGTCGCCGGGCTCGCCCTGCAAGGCCCCACCTCTTTCGCTGTGCTGCGCGAGGC
GGGCTTCGCCGGAGTCGAAAAACTCAAGGTCTTCGACCTTGCCGATTTCCCGCACGACGACACAACCGTCATCATCTCGC
GCACCGGCTTTACCGGCGATCTCGGCTACGAACTGTTCGTGCCGGCCGACAAGGCGCTGAGCCTCTGGGACCGGCTGATG
ACAGCGGGCGAGCTGCGCGGCATCCGCGCCGTCGGCTACACCGCGCTCAACCGCGCGCGGCTCGAGGCCGGGCTGATCGT
TGCCAATGCCGACTTCACCACCGCCGGGCACGCTATCCGCGCCGATCGCTTGCGCAAGCCCGACGAGATCGGGCTTGGCT
TCATGATCGATCCGGAGAAAACCCATTTCAACGGCCGCCGCGCCGTCCTCGAGGCGCGCGCCAAGCGCAAGCTGCGCCAT
GTGCTGGTCGGGCTGGAGATCGAAGGCAACATCCCCGCCGAACACGCCATGGTCTACCACAAGAAGCACCAGGAGGTCGG
ACTGGTCAGCGGCGCCATGTGGTCGCCCATGGCCAAGCGCAACATCGCCATCGCCTCGCTGGCGCGGCCCTATGGTGATA
CTCTCGTGGAAGACCTCTGGGTCGAGATCTACGCCATGCGCGAGCTGCAGTATCAGAAGCTGATGAAACGGGCCAAGGTG
GTGGCGCGGCCCTTCATCAAGCTCGACCGCCGCACCGCCAATCCGCCGGCGGATTTCTGA

Upstream 100 bases:

>100_bases
GCTATTCCTGGCCGGCGCCGGCTCGCATCCCGGTGGCGGCGTCTCCGGCGCTCCTGGCTTGAACGCCGCGCGGCGCATCA
TTGCGATGAAGGGATAGGTC

Downstream 100 bases:

>100_bases
CCATGGCCGGCGAAGCAGAGCTGGAGGCGGCCGAGCAGTGGGAGCTGGTCAACACGCCACTTGGCGAGAAATGGTCCGGG
CGCACGCGCTATGCCGCCGC

Product: aminomethyltransferase

Products: NA

Alternate protein names: Glycine cleavage system T protein [H]

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MAQATRKKPVAKTADAAVSARIAAQSHFRTLRLGTPFQPRLDALAKTQDWYNWAGYRAPHSLWDEELEYFAIRSQAALFD
ISPMTKYRIEGPDAEAFLDRVTLRDVTRLRPGRVHYTAWCDDEGFVLDDGTLFRLSPTRFRLCSQERHLPWLLDSAIGFD
VTVEEETEAVAGLALQGPTSFAVLREAGFAGVEKLKVFDLADFPHDDTTVIISRTGFTGDLGYELFVPADKALSLWDRLM
TAGELRGIRAVGYTALNRARLEAGLIVANADFTTAGHAIRADRLRKPDEIGLGFMIDPEKTHFNGRRAVLEARAKRKLRH
VLVGLEIEGNIPAEHAMVYHKKHQEVGLVSGAMWSPMAKRNIAIASLARPYGDTLVEDLWVEIYAMRELQYQKLMKRAKV
VARPFIKLDRRTANPPADF

Sequences:

>Translated_419_residues
MAQATRKKPVAKTADAAVSARIAAQSHFRTLRLGTPFQPRLDALAKTQDWYNWAGYRAPHSLWDEELEYFAIRSQAALFD
ISPMTKYRIEGPDAEAFLDRVTLRDVTRLRPGRVHYTAWCDDEGFVLDDGTLFRLSPTRFRLCSQERHLPWLLDSAIGFD
VTVEEETEAVAGLALQGPTSFAVLREAGFAGVEKLKVFDLADFPHDDTTVIISRTGFTGDLGYELFVPADKALSLWDRLM
TAGELRGIRAVGYTALNRARLEAGLIVANADFTTAGHAIRADRLRKPDEIGLGFMIDPEKTHFNGRRAVLEARAKRKLRH
VLVGLEIEGNIPAEHAMVYHKKHQEVGLVSGAMWSPMAKRNIAIASLARPYGDTLVEDLWVEIYAMRELQYQKLMKRAKV
VARPFIKLDRRTANPPADF
>Mature_418_residues
AQATRKKPVAKTADAAVSARIAAQSHFRTLRLGTPFQPRLDALAKTQDWYNWAGYRAPHSLWDEELEYFAIRSQAALFDI
SPMTKYRIEGPDAEAFLDRVTLRDVTRLRPGRVHYTAWCDDEGFVLDDGTLFRLSPTRFRLCSQERHLPWLLDSAIGFDV
TVEEETEAVAGLALQGPTSFAVLREAGFAGVEKLKVFDLADFPHDDTTVIISRTGFTGDLGYELFVPADKALSLWDRLMT
AGELRGIRAVGYTALNRARLEAGLIVANADFTTAGHAIRADRLRKPDEIGLGFMIDPEKTHFNGRRAVLEARAKRKLRHV
LVGLEIEGNIPAEHAMVYHKKHQEVGLVSGAMWSPMAKRNIAIASLARPYGDTLVEDLWVEIYAMRELQYQKLMKRAKVV
ARPFIKLDRRTANPPADF

Specific function: The glycine cleavage system catalyzes the degradation of glycine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gcvT family [H]

Homologues:

Organism=Homo sapiens, GI44662838, Length=363, Percent_Identity=26.4462809917355, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI257796258, Length=363, Percent_Identity=26.4462809917355, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI24797151, Length=308, Percent_Identity=25.3246753246753, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI257796256, Length=320, Percent_Identity=26.25, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1789272, Length=347, Percent_Identity=25.9365994236311, Blast_Score=102, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17560118, Length=344, Percent_Identity=27.3255813953488, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71994045, Length=345, Percent_Identity=28.1159420289855, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI71994052, Length=345, Percent_Identity=28.1159420289855, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI20129441, Length=383, Percent_Identity=27.4151436031332, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI20130091, Length=333, Percent_Identity=25.2252252252252, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013977
- InterPro:   IPR006222
- InterPro:   IPR006223
- InterPro:   IPR022903 [H]

Pfam domain/function: PF01571 GCV_T; PF08669 GCV_T_C [H]

EC number: =2.1.2.10 [H]

Molecular weight: Translated: 47105; Mature: 46973

Theoretical pI: Translated: 8.91; Mature: 8.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQATRKKPVAKTADAAVSARIAAQSHFRTLRLGTPFQPRLDALAKTQDWYNWAGYRAPH
CCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHCCHHHCCCCCCCH
SLWDEELEYFAIRSQAALFDISPMTKYRIEGPDAEAFLDRVTLRDVTRLRPGRVHYTAWC
HHHHHHHHHHEEECCCEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEEEE
DDEGFVLDDGTLFRLSPTRFRLCSQERHLPWLLDSAIGFDVTVEEETEAVAGLALQGPTS
CCCCEEECCCEEEEECHHHHHHHCCCCCCCEEEHHCCCCEEEECCCHHHHHCEEECCCCH
FAVLREAGFAGVEKLKVFDLADFPHDDTTVIISRTGFTGDLGYELFVPADKALSLWDRLM
HHHHHHCCCCCHHHEEEEEECCCCCCCCEEEEEECCCCCCCCEEEEECCHHHHHHHHHHH
TAGELRGIRAVGYTALNRARLEAGLIVANADFTTAGHAIRADRLRKPDEIGLGFMIDPEK
HHHHHCCCHHHHHHHHHHHHHEEEEEEECCCCCCCCCHHHHHHCCCCHHCCCCEEECCCC
THFNGRRAVLEARAKRKLRHVLVGLEIEGNIPAEHAMVYHKKHQEVGLVSGAMWSPMAKR
CCCCCHHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHCCCCCEECCCCCCCHHHC
NIAIASLARPYGDTLVEDLWVEIYAMRELQYQKLMKRAKVVARPFIKLDRRTANPPADF
CCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
AQATRKKPVAKTADAAVSARIAAQSHFRTLRLGTPFQPRLDALAKTQDWYNWAGYRAPH
CCCCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHCCHHHCCCCCCCH
SLWDEELEYFAIRSQAALFDISPMTKYRIEGPDAEAFLDRVTLRDVTRLRPGRVHYTAWC
HHHHHHHHHHEEECCCEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEEEE
DDEGFVLDDGTLFRLSPTRFRLCSQERHLPWLLDSAIGFDVTVEEETEAVAGLALQGPTS
CCCCEEECCCEEEEECHHHHHHHCCCCCCCEEEHHCCCCEEEECCCHHHHHCEEECCCCH
FAVLREAGFAGVEKLKVFDLADFPHDDTTVIISRTGFTGDLGYELFVPADKALSLWDRLM
HHHHHHCCCCCHHHEEEEEECCCCCCCCEEEEEECCCCCCCCEEEEECCHHHHHHHHHHH
TAGELRGIRAVGYTALNRARLEAGLIVANADFTTAGHAIRADRLRKPDEIGLGFMIDPEK
HHHHHCCCHHHHHHHHHHHHHEEEEEEECCCCCCCCCHHHHHHCCCCHHCCCCEEECCCC
THFNGRRAVLEARAKRKLRHVLVGLEIEGNIPAEHAMVYHKKHQEVGLVSGAMWSPMAKR
CCCCCHHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHCCCCCEECCCCCCCHHHC
NIAIASLARPYGDTLVEDLWVEIYAMRELQYQKLMKRAKVVARPFIKLDRRTANPPADF
CCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]