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The map label for this gene is fixL [H]

Identifier: 13473155

GI number: 13473155

Start: 2952330

End: 2954390

Strand: Direct

Name: fixL [H]

Synonym: mlr3659

Alternate gene names: 13473155

Gene position: 2952330-2954390 (Clockwise)

Preceding gene: 13473154

Following gene: 13473157

Centisome position: 41.96

GC content: 64.73

Gene sequence:

>2061_bases
ATGCAGGGTTTCAGGCAGGGCGCGGCGATGGCGGCCGTGCGCGTCGTTCGCCGGCTGCGCGAGGCCGGCGACTGGCAGCG
CGAGATGGTCGGCATACTGGAAACCCTTTGCCGGGCCATCGAATGCCAGCGCGGCATCCTGTTCCGCTTGCGCGAACTGC
CGGGCCAGGGTTTTGCCCAGTCGGTTGCCGCCTACTGGATCGATCCCGACTTTGGCGGCGAACTGGCCTCGCCGACCGTC
ATTGTGCAGTCGATCGTCAATTCGGATCCGCTGCTGGAGCGCGTCGCCGAGGACGAACGGCAGGGCAAGATCTTTGCCGG
GCATACGCGCAATCTCGAAGGGTTCCTGAGAACCGATTTCGAAAAGCAGAACATCAAGTCGTTCCTGTCGGTGTCGGTCT
TCGCCCATGGCCATCTGTGGGGCACGCTCGCCGTCAATGACTGCGTCGACGAGCGCGAATGGACCGACGAGGAAGAGGCG
ACGCTGCACATCATCGCGCTCGCCATCGGCGACGCCATCGAACACTCGCCCTCCGACGCCCATGCCAGCGAAGTGATCCG
CCGCACCATGCTGCAGGCTTCCCTCGACGCCATCATCGTCATCGACGAGGCCGGATCGATCATCGAATTCAATCCTGCCG
CCGAAAAGATGTTCGGCTTCCAGCGCAGCGATATCCTCGGCAAGGACCTGCTCGACACCGTCGTGCCGGAATACTACCGC
AAGGGCTATTCCTCGGGCGCCGACTACATGTCGGGCCGCGGTGCGCCGATGGTCGGCCAAAGGCTCGAGACCGTGACGCA
GAATGCCGCCGGCGAGGTGTTTCCGATCGAGCTGACGGCGACCGAGATGCGCGTCGCCGACCGCCGCCTGATCTTCGGCT
CGATCCGCGACCTGCGCGACAGGCTGCGCGCAGAGGAAGAGATCAACCGGCAGCGCGAGAAGCTGCACCAGAACGAGAAG
ATGGCGGCGATGGGCTCGCTGCTTGCCGGCGTTTCGCACGAGCTCAACAACCCGCTGGCGGTGGTGGTCGCGCAATCGAC
GCTGCTGCACGAATTCGCCTCCGACCCGCAGACCAAGGTCCGCGCCGAGAAGGTGCGTGCCGCGGCCGAGCGTTGCGGCC
GCATCGTCAAGAGCTTCCTGTCGATGGTCCGGCTTCATCCCGCGGCCCAGGCCGAGACCGATCTCAACCAGGTGATCCGC
GCTGCCCTTGAGGTGACCGCCTATGGCGCACGCTCGAGCGGCATCATCATCGACACCGATTTCGCGCCCGGTCCGCTGCT
GGCCATGGCGGACGCCGACCACGTGACGCAGGTGGCGGCCAATTTCCTCATCAACAGCCAGCACGCGCTGGCAGGCATTG
CCGGCGACCGGCTGATCAAGGTGCGGACCTTCCGCAGCGATCGCGACAATCCCTGCTTCTCGGTCGAGGACAACGGGCCT
GGCATTCCCGAAGCGATCCGCAACCGCATCTTCGAATCCTACTTCACCACCAAGCCGGTTGGTGTGGGCACCGGCATCGG
CCTGTCGATCTCGAAATCGATCATCGAGCGGCACAATGGCAATGTCTGGTTCGAGGAAGTGGAGCCCCGAGGCGCGCGTT
TCGTTGTCCAGTTGCCGCCCATTTCGACGGGCATCGCCGCTGCCGGCGAAAGCCAGCCACGCTCGAGCGGCCTGCGTCAT
GCCTTGATCATCGACGACGAGCCTGATGTCGCGGGCTCCTTGTCCGACATTCTCGAATTGATGGGCATCAAGTCGCGCAT
CGCGCCGGTGTGGGAATCGGGCGCCGCCACCTTGAGCGGCCACATACCGCCCGACATCGTCTTTTCGGACCTGCGCATGC
CCGGCACATCAGGCATGGCCATCTACCGCGAGCTGCTTGCCGAGCGGCCCGAACTGGCACGGCGTTTCGTGCTGGTCACC
GGCGATCTCATCGGCGCCAAGGCGGAAATCGAAGCCTTGCCGGCGCAGCAGCGGCCGCAGATCCTGGAAAAGCCGTTCAG
CACGCTGGACGTGCGCAGCGTGCTTTCATCCATTGCTGAGCAGGCCGCGTTGAAGGGCTAG

Upstream 100 bases:

>100_bases
ACAGGTTCACAGCCGTTGTTCGCCGATCGACTGGGCCGATCGGATTGACGTGACTTTGGAATAACGGCTGAAAATGACGG
GCGAAGCAACAAGCAGGGAT

Downstream 100 bases:

>100_bases
AGCAGTTGACCGTTTCACTGAAACGGCGAACGGCTCTAACTCTTTGTTTTGACGCAATTCCCAAGGAAAGCGCTACGCGC
TTTTCCCGGGGAAAAACCGC

Product: histidine protein kinase, FixL

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 686; Mature: 686

Protein sequence:

>686_residues
MQGFRQGAAMAAVRVVRRLREAGDWQREMVGILETLCRAIECQRGILFRLRELPGQGFAQSVAAYWIDPDFGGELASPTV
IVQSIVNSDPLLERVAEDERQGKIFAGHTRNLEGFLRTDFEKQNIKSFLSVSVFAHGHLWGTLAVNDCVDEREWTDEEEA
TLHIIALAIGDAIEHSPSDAHASEVIRRTMLQASLDAIIVIDEAGSIIEFNPAAEKMFGFQRSDILGKDLLDTVVPEYYR
KGYSSGADYMSGRGAPMVGQRLETVTQNAAGEVFPIELTATEMRVADRRLIFGSIRDLRDRLRAEEEINRQREKLHQNEK
MAAMGSLLAGVSHELNNPLAVVVAQSTLLHEFASDPQTKVRAEKVRAAAERCGRIVKSFLSMVRLHPAAQAETDLNQVIR
AALEVTAYGARSSGIIIDTDFAPGPLLAMADADHVTQVAANFLINSQHALAGIAGDRLIKVRTFRSDRDNPCFSVEDNGP
GIPEAIRNRIFESYFTTKPVGVGTGIGLSISKSIIERHNGNVWFEEVEPRGARFVVQLPPISTGIAAAGESQPRSSGLRH
ALIIDDEPDVAGSLSDILELMGIKSRIAPVWESGAATLSGHIPPDIVFSDLRMPGTSGMAIYRELLAERPELARRFVLVT
GDLIGAKAEIEALPAQQRPQILEKPFSTLDVRSVLSSIAEQAALKG

Sequences:

>Translated_686_residues
MQGFRQGAAMAAVRVVRRLREAGDWQREMVGILETLCRAIECQRGILFRLRELPGQGFAQSVAAYWIDPDFGGELASPTV
IVQSIVNSDPLLERVAEDERQGKIFAGHTRNLEGFLRTDFEKQNIKSFLSVSVFAHGHLWGTLAVNDCVDEREWTDEEEA
TLHIIALAIGDAIEHSPSDAHASEVIRRTMLQASLDAIIVIDEAGSIIEFNPAAEKMFGFQRSDILGKDLLDTVVPEYYR
KGYSSGADYMSGRGAPMVGQRLETVTQNAAGEVFPIELTATEMRVADRRLIFGSIRDLRDRLRAEEEINRQREKLHQNEK
MAAMGSLLAGVSHELNNPLAVVVAQSTLLHEFASDPQTKVRAEKVRAAAERCGRIVKSFLSMVRLHPAAQAETDLNQVIR
AALEVTAYGARSSGIIIDTDFAPGPLLAMADADHVTQVAANFLINSQHALAGIAGDRLIKVRTFRSDRDNPCFSVEDNGP
GIPEAIRNRIFESYFTTKPVGVGTGIGLSISKSIIERHNGNVWFEEVEPRGARFVVQLPPISTGIAAAGESQPRSSGLRH
ALIIDDEPDVAGSLSDILELMGIKSRIAPVWESGAATLSGHIPPDIVFSDLRMPGTSGMAIYRELLAERPELARRFVLVT
GDLIGAKAEIEALPAQQRPQILEKPFSTLDVRSVLSSIAEQAALKG
>Mature_686_residues
MQGFRQGAAMAAVRVVRRLREAGDWQREMVGILETLCRAIECQRGILFRLRELPGQGFAQSVAAYWIDPDFGGELASPTV
IVQSIVNSDPLLERVAEDERQGKIFAGHTRNLEGFLRTDFEKQNIKSFLSVSVFAHGHLWGTLAVNDCVDEREWTDEEEA
TLHIIALAIGDAIEHSPSDAHASEVIRRTMLQASLDAIIVIDEAGSIIEFNPAAEKMFGFQRSDILGKDLLDTVVPEYYR
KGYSSGADYMSGRGAPMVGQRLETVTQNAAGEVFPIELTATEMRVADRRLIFGSIRDLRDRLRAEEEINRQREKLHQNEK
MAAMGSLLAGVSHELNNPLAVVVAQSTLLHEFASDPQTKVRAEKVRAAAERCGRIVKSFLSMVRLHPAAQAETDLNQVIR
AALEVTAYGARSSGIIIDTDFAPGPLLAMADADHVTQVAANFLINSQHALAGIAGDRLIKVRTFRSDRDNPCFSVEDNGP
GIPEAIRNRIFESYFTTKPVGVGTGIGLSISKSIIERHNGNVWFEEVEPRGARFVVQLPPISTGIAAAGESQPRSSGLRH
ALIIDDEPDVAGSLSDILELMGIKSRIAPVWESGAATLSGHIPPDIVFSDLRMPGTSGMAIYRELLAERPELARRFVLVT
GDLIGAKAEIEALPAQQRPQILEKPFSTLDVRSVLSSIAEQAALKG

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=361, Percent_Identity=28.5318559556787, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1790436, Length=253, Percent_Identity=32.8063241106719, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI48994928, Length=494, Percent_Identity=22.4696356275304, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI1790300, Length=358, Percent_Identity=24.8603351955307, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1790346, Length=229, Percent_Identity=26.6375545851528, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1789808, Length=198, Percent_Identity=28.2828282828283, Blast_Score=73, Evalue=8e-14,
Organism=Escherichia coli, GI1786912, Length=246, Percent_Identity=29.6747967479675, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 75045; Mature: 75045

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS50112 PAS ; PS50110 RESPONSE_REGULATORY ; PS00037 MYB_1 ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGFRQGAAMAAVRVVRRLREAGDWQREMVGILETLCRAIECQRGILFRLRELPGQGFAQ
CCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
SVAAYWIDPDFGGELASPTVIVQSIVNSDPLLERVAEDERQGKIFAGHTRNLEGFLRTDF
HHHHEEECCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCEEECCCCCCCHHHHCCC
EKQNIKSFLSVSVFAHGHLWGTLAVNDCVDEREWTDEEEATLHIIALAIGDAIEHSPSDA
CHHHHHHHHHHHHEECCCEEEEEEHHHHHCCCCCCCCCCCEEEEEEEHHHHHHHCCCCHH
HASEVIRRTMLQASLDAIIVIDEAGSIIEFNPAAEKMFGFQRSDILGKDLLDTVVPEYYR
HHHHHHHHHHHHHCCCEEEEEECCCCEEEECHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
KGYSSGADYMSGRGAPMVGQRLETVTQNAAGEVFPIELTATEMRVADRRLIFGSIRDLRD
HCCCCCCCHHCCCCCCHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHH
RLRAEEEINRQREKLHQNEKMAAMGSLLAGVSHELNNPLAVVVAQSTLLHEFASDPQTKV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHCCCHHHH
RAEKVRAAAERCGRIVKSFLSMVRLHPAAQAETDLNQVIRAALEVTAYGARSSGIIIDTD
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECC
FAPGPLLAMADADHVTQVAANFLINSQHALAGIAGDRLIKVRTFRSDRDNPCFSVEDNGP
CCCCCEEEECCCHHHHHHHHHHHHCCCHHHHCCCCCCEEEEEEECCCCCCCCEEECCCCC
GIPEAIRNRIFESYFTTKPVGVGTGIGLSISKSIIERHNGNVWFEEVEPRGARFVVQLPP
CCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCC
ISTGIAAAGESQPRSSGLRHALIIDDEPDVAGSLSDILELMGIKSRIAPVWESGAATLSG
CCCCCCCCCCCCCHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCHHHCCCCCEECC
HIPPDIVFSDLRMPGTSGMAIYRELLAERPELARRFVLVTGDLIGAKAEIEALPAQQRPQ
CCCHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHEEEEEECCHHCCCHHHHHCCCCCCCH
ILEKPFSTLDVRSVLSSIAEQAALKG
HHHCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQGFRQGAAMAAVRVVRRLREAGDWQREMVGILETLCRAIECQRGILFRLRELPGQGFAQ
CCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
SVAAYWIDPDFGGELASPTVIVQSIVNSDPLLERVAEDERQGKIFAGHTRNLEGFLRTDF
HHHHEEECCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCEEECCCCCCCHHHHCCC
EKQNIKSFLSVSVFAHGHLWGTLAVNDCVDEREWTDEEEATLHIIALAIGDAIEHSPSDA
CHHHHHHHHHHHHEECCCEEEEEEHHHHHCCCCCCCCCCCEEEEEEEHHHHHHHCCCCHH
HASEVIRRTMLQASLDAIIVIDEAGSIIEFNPAAEKMFGFQRSDILGKDLLDTVVPEYYR
HHHHHHHHHHHHHCCCEEEEEECCCCEEEECHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
KGYSSGADYMSGRGAPMVGQRLETVTQNAAGEVFPIELTATEMRVADRRLIFGSIRDLRD
HCCCCCCCHHCCCCCCHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHH
RLRAEEEINRQREKLHQNEKMAAMGSLLAGVSHELNNPLAVVVAQSTLLHEFASDPQTKV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHCCCHHHH
RAEKVRAAAERCGRIVKSFLSMVRLHPAAQAETDLNQVIRAALEVTAYGARSSGIIIDTD
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECC
FAPGPLLAMADADHVTQVAANFLINSQHALAGIAGDRLIKVRTFRSDRDNPCFSVEDNGP
CCCCCEEEECCCHHHHHHHHHHHHCCCHHHHCCCCCCEEEEEEECCCCCCCCEEECCCCC
GIPEAIRNRIFESYFTTKPVGVGTGIGLSISKSIIERHNGNVWFEEVEPRGARFVVQLPP
CCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCC
ISTGIAAAGESQPRSSGLRHALIIDDEPDVAGSLSDILELMGIKSRIAPVWESGAATLSG
CCCCCCCCCCCCCHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCHHHCCCCCEECC
HIPPDIVFSDLRMPGTSGMAIYRELLAERPELARRFVLVTGDLIGAKAEIEALPAQQRPQ
CCCHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHEEEEEECCHHCCCHHHHHCCCCCCCH
ILEKPFSTLDVRSVLSSIAEQAALKG
HHHCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2842062; 11481432; 8432704 [H]