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The map label for this gene is pdhC [H]

Identifier: 13473129

GI number: 13473129

Start: 2924456

End: 2925820

Strand: Reverse

Name: pdhC [H]

Synonym: mll3627

Alternate gene names: 13473129

Gene position: 2925820-2924456 (Counterclockwise)

Preceding gene: 13473130

Following gene: 13473128

Centisome position: 41.58

GC content: 64.03

Gene sequence:

>1365_bases
ATGCCGACCGAAGTCATTCTCCCCAAGGTCGATATGGACATGGCGACCGGACAGATCTCGCGCTGGTTCGCCGAAGAAGG
TGCCCGGGTCAAGAAGGGCGATGTGCTGTTCGAGATCGAGACCGACAAGGCGGCCATGGAGATCGATGCGCCGGCCAGCG
GCGTGCTGCGCGACGTCTCAGGCAAGGAAGGCGTCGACATTCCCGTCGGCGCGCCGGTTGCGTGGATTTACGCCGACGAT
GAGGCTTATGGGGCCAAGCAAGACGCGGCGCCAATCTCCCCCCTTGTGGGGGAGATGTCGGCGAAGTCGACAGAGGGGGA
CGTCGTCCCACCAACGTCGCATTCTGTCATGCCCCCCTCTGCCCTGCCGGGCATCTCCCCCACAAGGGGGGAGATTGGCC
AATCACCGCCAGGCGAACGCGCGACACCGCTTGCCCGACGTCTGGCTCGTGAGGCAGGTCTTGCCCTTGCCGGCATCATC
GGCACCGGCCCGCATGGCCGTGTGGTGAAGGCCGATGTCGATGCAGCGATTGCCGGCGGTGGTGCCAAGGCAGCGCCCGC
CGCCAAGGCTCCGGCCGGTGCGCCGGCTGCCGCTCCCGCGCCGGCTGTGAAGCCGATGTCGGACGACCAAGTGCTGAAGC
TGTTCGAGCAAGGCTCCTACGAACTCGTCCCGCACGACAATATGCGCAAGACCATCGCGCGCCGCCTGGTCGAGGCCAAG
TCCACCATCCCGCATTTTTATCTAACGCTCGACTGCGAACTCGATGCGTTGCTGGCCTTGCGCACGCAGATCAATGCCGC
CGCACCGATGAAGAAGACCGAAAAGGGTGACGTGCCTGCCTACAAGCTGTCGGTCAACGACATGGTGATCAAGGCGATGG
CGATGGCGCTGAAGGCGGTGCCGGATGCCAATGCCTCGTGGACCGAAACCGCCATGGTCAAGCACAAGCATGCCGATGTC
GGCGTCGCCGTGTCGATCCCCGGCGGCCTGATCACGCCGATCATTCGGCATGCGGACGAAAAAACGCTGTCGACCATTTC
CAACGAGATGAAGGACCTGGCCAGCCGTGCCCGCAGCCGCAAGCTGAAGCCGGAAGAGTATCAGGGCGGCACGACGGCGG
TCTCCAACCTCGGCATGTTCGGCATCAAGGACTTCGCCGCCGTCATCAATCCGCCGCATGCGACGATCCTGGCGGTCGGC
GCCGGTGAAGAGCGGGCTGTGGTCAAGAATGGCGAGATCAAAATCGCCACCGTGATGTCGGTGACGCTGTCGACCGATCA
TCGCGCCGTTGATGGCGCGCTCGGCGCCGAATTGTTGGTCGCCTTCAAGCGGCTGATCGAGAACCCGATGGGCATGCTGG
TGTAG

Upstream 100 bases:

>100_bases
GCCGATCCCCTACAATCCGGAGCTCGAAAAGGCCACGGTGCCGCAGGTTCCCGACATCATCAGCGCCGCGCGCGACCTCG
TGAAAGGGGTTCGCTGAGAC

Downstream 100 bases:

>100_bases
GGGCAGTCGAGGCACCTTTTACCAGCATCGGGAAACATAGCTCCTAGTCCGGCGTGCAGTCGCGCCCATGAGGCTCGCGG
AAAGCCAATTCCGTGGGCCT

Product: dihydrolipoamide acetyltransferase homoserine dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MPTEVILPKVDMDMATGQISRWFAEEGARVKKGDVLFEIETDKAAMEIDAPASGVLRDVSGKEGVDIPVGAPVAWIYADD
EAYGAKQDAAPISPLVGEMSAKSTEGDVVPPTSHSVMPPSALPGISPTRGEIGQSPPGERATPLARRLAREAGLALAGII
GTGPHGRVVKADVDAAIAGGGAKAAPAAKAPAGAPAAAPAPAVKPMSDDQVLKLFEQGSYELVPHDNMRKTIARRLVEAK
STIPHFYLTLDCELDALLALRTQINAAAPMKKTEKGDVPAYKLSVNDMVIKAMAMALKAVPDANASWTETAMVKHKHADV
GVAVSIPGGLITPIIRHADEKTLSTISNEMKDLASRARSRKLKPEEYQGGTTAVSNLGMFGIKDFAAVINPPHATILAVG
AGEERAVVKNGEIKIATVMSVTLSTDHRAVDGALGAELLVAFKRLIENPMGMLV

Sequences:

>Translated_454_residues
MPTEVILPKVDMDMATGQISRWFAEEGARVKKGDVLFEIETDKAAMEIDAPASGVLRDVSGKEGVDIPVGAPVAWIYADD
EAYGAKQDAAPISPLVGEMSAKSTEGDVVPPTSHSVMPPSALPGISPTRGEIGQSPPGERATPLARRLAREAGLALAGII
GTGPHGRVVKADVDAAIAGGGAKAAPAAKAPAGAPAAAPAPAVKPMSDDQVLKLFEQGSYELVPHDNMRKTIARRLVEAK
STIPHFYLTLDCELDALLALRTQINAAAPMKKTEKGDVPAYKLSVNDMVIKAMAMALKAVPDANASWTETAMVKHKHADV
GVAVSIPGGLITPIIRHADEKTLSTISNEMKDLASRARSRKLKPEEYQGGTTAVSNLGMFGIKDFAAVINPPHATILAVG
AGEERAVVKNGEIKIATVMSVTLSTDHRAVDGALGAELLVAFKRLIENPMGMLV
>Mature_453_residues
PTEVILPKVDMDMATGQISRWFAEEGARVKKGDVLFEIETDKAAMEIDAPASGVLRDVSGKEGVDIPVGAPVAWIYADDE
AYGAKQDAAPISPLVGEMSAKSTEGDVVPPTSHSVMPPSALPGISPTRGEIGQSPPGERATPLARRLAREAGLALAGIIG
TGPHGRVVKADVDAAIAGGGAKAAPAAKAPAGAPAAAPAPAVKPMSDDQVLKLFEQGSYELVPHDNMRKTIARRLVEAKS
TIPHFYLTLDCELDALLALRTQINAAAPMKKTEKGDVPAYKLSVNDMVIKAMAMALKAVPDANASWTETAMVKHKHADVG
VAVSIPGGLITPIIRHADEKTLSTISNEMKDLASRARSRKLKPEEYQGGTTAVSNLGMFGIKDFAAVINPPHATILAVGA
GEERAVVKNGEIKIATVMSVTLSTDHRAVDGALGAELLVAFKRLIENPMGMLV

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=455, Percent_Identity=38.9010989010989, Blast_Score=290, Evalue=2e-78,
Organism=Homo sapiens, GI203098816, Length=476, Percent_Identity=34.453781512605, Blast_Score=224, Evalue=9e-59,
Organism=Homo sapiens, GI203098753, Length=475, Percent_Identity=34.5263157894737, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI260898739, Length=160, Percent_Identity=43.75, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI110671329, Length=444, Percent_Identity=27.027027027027, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI19923748, Length=231, Percent_Identity=30.7359307359307, Blast_Score=110, Evalue=2e-24,
Organism=Escherichia coli, GI1786946, Length=451, Percent_Identity=29.0465631929047, Blast_Score=162, Evalue=5e-41,
Organism=Escherichia coli, GI1786305, Length=426, Percent_Identity=29.1079812206573, Blast_Score=144, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17560088, Length=462, Percent_Identity=40.2597402597403, Blast_Score=278, Evalue=5e-75,
Organism=Caenorhabditis elegans, GI17538894, Length=315, Percent_Identity=33.968253968254, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17537937, Length=455, Percent_Identity=26.3736263736264, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI25146366, Length=450, Percent_Identity=27.5555555555556, Blast_Score=130, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6324258, Length=464, Percent_Identity=36.4224137931034, Blast_Score=248, Evalue=1e-66,
Organism=Saccharomyces cerevisiae, GI6320352, Length=232, Percent_Identity=32.7586206896552, Blast_Score=121, Evalue=2e-28,
Organism=Drosophila melanogaster, GI20129315, Length=459, Percent_Identity=37.2549019607843, Blast_Score=246, Evalue=3e-65,
Organism=Drosophila melanogaster, GI24582497, Length=452, Percent_Identity=37.3893805309735, Blast_Score=244, Evalue=1e-64,
Organism=Drosophila melanogaster, GI18859875, Length=462, Percent_Identity=28.5714285714286, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24645909, Length=231, Percent_Identity=32.4675324675325, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 47490; Mature: 47359

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTEVILPKVDMDMATGQISRWFAEEGARVKKGDVLFEIETDKAAMEIDAPASGVLRDVS
CCCCEECCCCCCCHHHHHHHHHHHHCCCEEECCCEEEEEECCCEEEEECCCCHHHHHCCC
GKEGVDIPVGAPVAWIYADDEAYGAKQDAAPISPLVGEMSAKSTEGDVVPPTSHSVMPPS
CCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCC
ALPGISPTRGEIGQSPPGERATPLARRLAREAGLALAGIIGTGPHGRVVKADVDAAIAGG
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEEECCCCCCCEEEECCCEEEECC
GAKAAPAAKAPAGAPAAAPAPAVKPMSDDQVLKLFEQGSYELVPHDNMRKTIARRLVEAK
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHH
STIPHFYLTLDCELDALLALRTQINAAAPMKKTEKGDVPAYKLSVNDMVIKAMAMALKAV
HCCCEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHC
PDANASWTETAMVKHKHADVGVAVSIPGGLITPIIRHADEKTLSTISNEMKDLASRARSR
CCCCCCCHHHHHHHCCCCCCEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHC
KLKPEEYQGGTTAVSNLGMFGIKDFAAVINPPHATILAVGAGEERAVVKNGEIKIATVMS
CCCCHHHCCCHHHHHHCCCHHHHHHHHHCCCCCEEEEEEECCCCCEEEECCCEEEEEEEE
VTLSTDHRAVDGALGAELLVAFKRLIENPMGMLV
EEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
PTEVILPKVDMDMATGQISRWFAEEGARVKKGDVLFEIETDKAAMEIDAPASGVLRDVS
CCCEECCCCCCCHHHHHHHHHHHHCCCEEECCCEEEEEECCCEEEEECCCCHHHHHCCC
GKEGVDIPVGAPVAWIYADDEAYGAKQDAAPISPLVGEMSAKSTEGDVVPPTSHSVMPPS
CCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCC
ALPGISPTRGEIGQSPPGERATPLARRLAREAGLALAGIIGTGPHGRVVKADVDAAIAGG
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEEECCCCCCCEEEECCCEEEECC
GAKAAPAAKAPAGAPAAAPAPAVKPMSDDQVLKLFEQGSYELVPHDNMRKTIARRLVEAK
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHH
STIPHFYLTLDCELDALLALRTQINAAAPMKKTEKGDVPAYKLSVNDMVIKAMAMALKAV
HCCCEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHC
PDANASWTETAMVKHKHADVGVAVSIPGGLITPIIRHADEKTLSTISNEMKDLASRARSR
CCCCCCCHHHHHHHCCCCCCEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHC
KLKPEEYQGGTTAVSNLGMFGIKDFAAVINPPHATILAVGAGEERAVVKNGEIKIATVMS
CCCCHHHCCCHHHHHHCCCHHHHHHHHHCCCCCEEEEEEECCCCCEEEECCCEEEEEEEE
VTLSTDHRAVDGALGAELLVAFKRLIENPMGMLV
EEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]