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The map label for this gene is gloB

Identifier: 13473061

GI number: 13473061

Start: 2860413

End: 2861180

Strand: Direct

Name: gloB

Synonym: mlr3546

Alternate gene names: 13473061

Gene position: 2860413-2861180 (Clockwise)

Preceding gene: 13473055

Following gene: 13473062

Centisome position: 40.65

GC content: 62.37

Gene sequence:

>768_bases
ATGGCGGTCGAAATCGAACAATTCATGTGCCGGAGCGACAATTTCGGCGTGCTGGTGCATGACCCCAAGAGCGGGCAGAC
CGCGATCATCGACGCACCCGAGGAAGCGCCCATCCTGGCCGCGATCAAGCGCACCGGCTGGACGCCGACGATGATCCTGA
CCACGCATCATCATATGGACCACGTCGAGGCCAATCTGGCGCTGAAGGAGCGCTTCAAGCTGCGCATCGTCGGCCCGGAG
GCGGAAAAGGCCAAGATCCCCGGCATCGACGAAACCGTCGAGGAGGGCTCCGTCCTTCATCTCGGCGATGAGCGCATCGA
GGTGATCGCCACCCCCGGCCATACCGCCGGTCATGTCTCCTATCATCTGCCGGCATCGAAGGTGGCCTTCACCGCCGACA
CGCTGTTCGCGCTGGGCTGTGGGCGGCTGTTCGAATGCAAGCCGCCGGTGATGTATGAATCGCTGCGGAAACTCGCAGCC
CTTCCCGCCGCGACGACAATCTATTGCGGCCATGAATACACGCTTGCCAACGCCCGTTTCGCGCTGACCGTCGACCCGAC
CAATTCGGCATTGAAGGAGCGCGCCACCAGGATCGAGGCGCTGCGGGTCGACAACAAGCCGACACTGCCGACGACGATCG
GCGAGGAACTGTCGACCAATCCCTTCCTGCGCTGGCACGATCCGGCGATCCGCAAGCATCTTGGCATGGAAAAGGCCGGG
GATGCCGAGGTGTTCGCCGAAATTCGCAAGCGCAAGGACAATTTCTGA

Upstream 100 bases:

>100_bases
GCGATGACATTTGTCCTCCCCGGGAATGACATTCGGTCACCCCGTAGATGACATCCGCCCTCCGGCGCCCTATGTCTGCG
GCGACAAAAGGAGAGACGCG

Downstream 100 bases:

>100_bases
GCACCTTCGACCGATGACCAGTTCAGCAGAAATCATCGCGACACTCGGACTGAAGCCGCATCCGGAAGGCGGCTGGTACG
CGGAGACCTTTCGCGACGGG

Product: putative glyoxalase II

Products: NA

Alternate protein names: Glyoxalase II; Glx II

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MAVEIEQFMCRSDNFGVLVHDPKSGQTAIIDAPEEAPILAAIKRTGWTPTMILTTHHHMDHVEANLALKERFKLRIVGPE
AEKAKIPGIDETVEEGSVLHLGDERIEVIATPGHTAGHVSYHLPASKVAFTADTLFALGCGRLFECKPPVMYESLRKLAA
LPAATTIYCGHEYTLANARFALTVDPTNSALKERATRIEALRVDNKPTLPTTIGEELSTNPFLRWHDPAIRKHLGMEKAG
DAEVFAEIRKRKDNF

Sequences:

>Translated_255_residues
MAVEIEQFMCRSDNFGVLVHDPKSGQTAIIDAPEEAPILAAIKRTGWTPTMILTTHHHMDHVEANLALKERFKLRIVGPE
AEKAKIPGIDETVEEGSVLHLGDERIEVIATPGHTAGHVSYHLPASKVAFTADTLFALGCGRLFECKPPVMYESLRKLAA
LPAATTIYCGHEYTLANARFALTVDPTNSALKERATRIEALRVDNKPTLPTTIGEELSTNPFLRWHDPAIRKHLGMEKAG
DAEVFAEIRKRKDNF
>Mature_254_residues
AVEIEQFMCRSDNFGVLVHDPKSGQTAIIDAPEEAPILAAIKRTGWTPTMILTTHHHMDHVEANLALKERFKLRIVGPEA
EKAKIPGIDETVEEGSVLHLGDERIEVIATPGHTAGHVSYHLPASKVAFTADTLFALGCGRLFECKPPVMYESLRKLAAL
PAATTIYCGHEYTLANARFALTVDPTNSALKERATRIEALRVDNKPTLPTTIGEELSTNPFLRWHDPAIRKHLGMEKAGD
AEVFAEIRKRKDNF

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family

Homologues:

Organism=Homo sapiens, GI94538320, Length=260, Percent_Identity=32.6923076923077, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI94538322, Length=260, Percent_Identity=32.6923076923077, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI14150041, Length=249, Percent_Identity=34.9397590361446, Blast_Score=143, Evalue=1e-34,
Organism=Homo sapiens, GI21703352, Length=255, Percent_Identity=34.5098039215686, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI116642887, Length=255, Percent_Identity=34.5098039215686, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI46361987, Length=194, Percent_Identity=34.020618556701, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI41327741, Length=172, Percent_Identity=30.2325581395349, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI1786406, Length=246, Percent_Identity=37.8048780487805, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1787158, Length=134, Percent_Identity=29.1044776119403, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17536925, Length=265, Percent_Identity=34.3396226415094, Blast_Score=142, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17538952, Length=170, Percent_Identity=27.6470588235294, Blast_Score=68, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6320478, Length=246, Percent_Identity=32.1138211382114, Blast_Score=98, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6324614, Length=264, Percent_Identity=29.9242424242424, Blast_Score=97, Evalue=3e-21,
Organism=Drosophila melanogaster, GI21356335, Length=264, Percent_Identity=38.2575757575758, Blast_Score=181, Evalue=4e-46,
Organism=Drosophila melanogaster, GI24667711, Length=264, Percent_Identity=38.2575757575758, Blast_Score=181, Evalue=6e-46,
Organism=Drosophila melanogaster, GI24667703, Length=264, Percent_Identity=38.2575757575758, Blast_Score=180, Evalue=9e-46,
Organism=Drosophila melanogaster, GI221330176, Length=176, Percent_Identity=32.9545454545455, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLO2_RHILO (Q98G02)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_104628.1
- HSSP:   Q9SID3
- ProteinModelPortal:   Q98G02
- SMR:   Q98G02
- GeneID:   1227289
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr3546
- NMPDR:   fig|266835.1.peg.2732
- HOGENOM:   HBG753931
- OMA:   WCAHEYT
- ProtClustDB:   CLSK863794
- BRENDA:   3.1.2.6
- HAMAP:   MF_01374
- InterPro:   IPR001279
- InterPro:   IPR017782
- SMART:   SM00849
- TIGRFAMs:   TIGR03413

Pfam domain/function: PF00753 Lactamase_B

EC number: =3.1.2.6

Molecular weight: Translated: 28195; Mature: 28063

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVEIEQFMCRSDNFGVLVHDPKSGQTAIIDAPEEAPILAAIKRTGWTPTMILTTHHHMD
CEEEHHHHHHCCCCCEEEEECCCCCCEEEEECCCCCCHHEEHHHCCCCCEEEEEECCCHH
HVEANLALKERFKLRIVGPEAEKAKIPGIDETVEEGSVLHLGDERIEVIATPGHTAGHVS
HHHHHEEEEECEEEEEECCCCCCCCCCCCCHHHHCCCEEEECCCEEEEEECCCCCCCEEE
YHLPASKVAFTADTLFALGCGRLFECKPPVMYESLRKLAALPAATTIYCGHEYTLANARF
EECCCHHEEEHHHHHHHHCCCCEEECCCCHHHHHHHHHHHCCCCEEEEECCEEEECCCEE
ALTVDPTNSALKERATRIEALRVDNKPTLPTTIGEELSTNPFLRWHDPAIRKHLGMEKAG
EEEECCCHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCCCEEECCHHHHHHCCCCCCC
DAEVFAEIRKRKDNF
CHHHHHHHHHHCCCC
>Mature Secondary Structure 
AVEIEQFMCRSDNFGVLVHDPKSGQTAIIDAPEEAPILAAIKRTGWTPTMILTTHHHMD
EEEHHHHHHCCCCCEEEEECCCCCCEEEEECCCCCCHHEEHHHCCCCCEEEEEECCCHH
HVEANLALKERFKLRIVGPEAEKAKIPGIDETVEEGSVLHLGDERIEVIATPGHTAGHVS
HHHHHEEEEECEEEEEECCCCCCCCCCCCCHHHHCCCEEEECCCEEEEEECCCCCCCEEE
YHLPASKVAFTADTLFALGCGRLFECKPPVMYESLRKLAALPAATTIYCGHEYTLANARF
EECCCHHEEEHHHHHHHHCCCCEEECCCCHHHHHHHHHHHCCCCEEEEECCEEEECCCEE
ALTVDPTNSALKERATRIEALRVDNKPTLPTTIGEELSTNPFLRWHDPAIRKHLGMEKAG
EEEECCCHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCCCEEECCHHHHHHCCCCCCC
DAEVFAEIRKRKDNF
CHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968