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The map label for this gene is yjjN [H]

Identifier: 13472897

GI number: 13472897

Start: 2686920

End: 2687936

Strand: Direct

Name: yjjN [H]

Synonym: mlr3340

Alternate gene names: 13472897

Gene position: 2686920-2687936 (Clockwise)

Preceding gene: 13472896

Following gene: 13472899

Centisome position: 38.19

GC content: 65.68

Gene sequence:

>1017_bases
ATGAAAGCCGTTATCTGCCGCTCGCCCGGGGAACTCGTCCTGGAAGACCGCCCCGCGCCCGGCGCGCCTCCTGCCGGCTG
GGCGCTGGTCGCCGTCAGCCATGTCGGCATCTGCGGCACCGACTACCACATTTTCGAGGGCAAGCACCCGTTTCTCGCCT
ACCCCCGCATCATGGGCCATGAAGTCTCCGGCACCGTCATCGAGACCGGCGAGGGCCCCGACCTTGCCATTGGCGAACCG
GTCGTCATCAACCCCTACCTCTCCTGCGGCAAGTGCATCGCCTGCCGGCACGGCAAACCGAATTGCTGCGTCAAGATCGA
GGTGCTGGGCGTCCATCGCGACGGCGCCATGTGCGAACGCATTCTTGTCCCGGCGCAGAATCTCTATCCGGCCAATGGCC
TGTCGCTGGCCGACGCCGCCGCTGTCGAATTCCTGGCCATCGGCGCGCATGCGGTACGTCGCGCCCGCGCCGATTCTGGC
GCCCGCGCGCTGGTCATCGGCGCCGGCCCGATCGGGCTCGGCAACGCCATCTTCGCCCGCATCGCCGGGCTCGACGTGAC
GCTGCTCGACATGAGCAGTGAACGCCTCGCATTCGCCGAAAACGAACTTGGGTTCAGTATCCTCGACGGCTCGCGCGGAG
CGGTGCCAGACCTGGTCAAGGACGCGACATCAGGCGAAGGCTTCGACGTGGTCTTCGACGCCACCGGCAACACCCGGTCG
GTGCAGTCGGCCTTCGCCCATGTGGCGCATGGCGGCACACTGGTGCTGGTCAGCGTCGTCAAGGACGACATCGTCTTTTC
CGATCCGGAATTCCACAAACGCGAAATGACGCTGGTCGGCAGTCGCAATGCCTTGCGTGCCGATTTCGATCATGTCGCTG
CCTCGATCCGAAACGGCGCGGTGCCGTTGGCCAAGCTCGTCACCCACCGCACCACGCTTGCCGATACGCCGAGCGATCTC
GCCCGCTGGGCGCACGAGAAATCCGGCCTGATCAAGGCCGTGATCGAGATCGGATAG

Upstream 100 bases:

>100_bases
TATTTCGACGGTCTGCGCCGCCGCGGCGGCAAGTGACCACTGCTGAAATCGATCTCCGGCCCGGCGTGAAGACGGGTCGG
AAACCCAACTGGACTGAAAC

Downstream 100 bases:

>100_bases
TCGGCAGAATCCGGCTCACAGCGCCGACAATTTCAACTCCACCCGTTCCGCCGGAAAGCGCGACTCCGAAAACTGGATCG
GCTGGCCGTCCGGCGTGATG

Product: L-iditol 2-dehydrogenase

Products: L-sorbose; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MKAVICRSPGELVLEDRPAPGAPPAGWALVAVSHVGICGTDYHIFEGKHPFLAYPRIMGHEVSGTVIETGEGPDLAIGEP
VVINPYLSCGKCIACRHGKPNCCVKIEVLGVHRDGAMCERILVPAQNLYPANGLSLADAAAVEFLAIGAHAVRRARADSG
ARALVIGAGPIGLGNAIFARIAGLDVTLLDMSSERLAFAENELGFSILDGSRGAVPDLVKDATSGEGFDVVFDATGNTRS
VQSAFAHVAHGGTLVLVSVVKDDIVFSDPEFHKREMTLVGSRNALRADFDHVAASIRNGAVPLAKLVTHRTTLADTPSDL
ARWAHEKSGLIKAVIEIG

Sequences:

>Translated_338_residues
MKAVICRSPGELVLEDRPAPGAPPAGWALVAVSHVGICGTDYHIFEGKHPFLAYPRIMGHEVSGTVIETGEGPDLAIGEP
VVINPYLSCGKCIACRHGKPNCCVKIEVLGVHRDGAMCERILVPAQNLYPANGLSLADAAAVEFLAIGAHAVRRARADSG
ARALVIGAGPIGLGNAIFARIAGLDVTLLDMSSERLAFAENELGFSILDGSRGAVPDLVKDATSGEGFDVVFDATGNTRS
VQSAFAHVAHGGTLVLVSVVKDDIVFSDPEFHKREMTLVGSRNALRADFDHVAASIRNGAVPLAKLVTHRTTLADTPSDL
ARWAHEKSGLIKAVIEIG
>Mature_338_residues
MKAVICRSPGELVLEDRPAPGAPPAGWALVAVSHVGICGTDYHIFEGKHPFLAYPRIMGHEVSGTVIETGEGPDLAIGEP
VVINPYLSCGKCIACRHGKPNCCVKIEVLGVHRDGAMCERILVPAQNLYPANGLSLADAAAVEFLAIGAHAVRRARADSG
ARALVIGAGPIGLGNAIFARIAGLDVTLLDMSSERLAFAENELGFSILDGSRGAVPDLVKDATSGEGFDVVFDATGNTRS
VQSAFAHVAHGGTLVLVSVVKDDIVFSDPEFHKREMTLVGSRNALRADFDHVAASIRNGAVPLAKLVTHRTTLADTPSDL
ARWAHEKSGLIKAVIEIG

Specific function: Putative L-galactonate oxidoreductase that is required for growth on L-galactonate as the sole carbon source [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=323, Percent_Identity=28.7925696594427, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI226510992, Length=336, Percent_Identity=40.7738095238095, Blast_Score=248, Evalue=4e-67,
Organism=Escherichia coli, GI1787863, Length=316, Percent_Identity=31.0126582278481, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1788073, Length=344, Percent_Identity=27.3255813953488, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1788407, Length=341, Percent_Identity=27.2727272727273, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1790045, Length=296, Percent_Identity=26.3513513513513, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1790718, Length=319, Percent_Identity=28.2131661442006, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1786825, Length=266, Percent_Identity=27.8195488721804, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI87082125, Length=334, Percent_Identity=25.4491017964072, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1788075, Length=329, Percent_Identity=22.7963525835866, Blast_Score=75, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17562876, Length=338, Percent_Identity=31.6568047337278, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17562878, Length=197, Percent_Identity=32.994923857868, Blast_Score=90, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6322619, Length=327, Percent_Identity=28.1345565749235, Blast_Score=120, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6319955, Length=327, Percent_Identity=28.1345565749235, Blast_Score=119, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6319257, Length=348, Percent_Identity=27.8735632183908, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6323099, Length=347, Percent_Identity=25.6484149855908, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6319258, Length=352, Percent_Identity=25.5681818181818, Blast_Score=96, Evalue=8e-21,
Organism=Drosophila melanogaster, GI17737897, Length=338, Percent_Identity=28.698224852071, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI17137530, Length=341, Percent_Identity=29.0322580645161, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI17737895, Length=292, Percent_Identity=27.0547945205479, Blast_Score=78, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.1.1.14

Molecular weight: Translated: 35471; Mature: 35471

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAVICRSPGELVLEDRPAPGAPPAGWALVAVSHVGICGTDYHIFEGKHPFLAYPRIMGH
CCEEEECCCCCEEEECCCCCCCCCCCEEEEEEECCCCCCCCEEEECCCCCCEECCHHHCC
EVSGTVIETGEGPDLAIGEPVVINPYLSCGKCIACRHGKPNCCVKIEVLGVHRDGAMCER
CCCCEEEECCCCCCEECCCCEEECCCCCCCCEEEECCCCCCEEEEEEEEEEECCCHHHHH
ILVPAQNLYPANGLSLADAAAVEFLAIGAHAVRRARADSGARALVIGAGPIGLGNAIFAR
HHCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHH
IAGLDVTLLDMSSERLAFAENELGFSILDGSRGAVPDLVKDATSGEGFDVVFDATGNTRS
HCCCEEEEEECCCCCEEEECCCCCEEEECCCCCCCCHHHHHCCCCCCEEEEEECCCCCHH
VQSAFAHVAHGGTLVLVSVVKDDIVFSDPEFHKREMTLVGSRNALRADFDHVAASIRNGA
HHHHHHHHHCCCEEEEEEEECCCEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCC
VPLAKLVTHRTTLADTPSDLARWAHEKSGLIKAVIEIG
CHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEECC
>Mature Secondary Structure
MKAVICRSPGELVLEDRPAPGAPPAGWALVAVSHVGICGTDYHIFEGKHPFLAYPRIMGH
CCEEEECCCCCEEEECCCCCCCCCCCEEEEEEECCCCCCCCEEEECCCCCCEECCHHHCC
EVSGTVIETGEGPDLAIGEPVVINPYLSCGKCIACRHGKPNCCVKIEVLGVHRDGAMCER
CCCCEEEECCCCCCEECCCCEEECCCCCCCCEEEECCCCCCEEEEEEEEEEECCCHHHHH
ILVPAQNLYPANGLSLADAAAVEFLAIGAHAVRRARADSGARALVIGAGPIGLGNAIFAR
HHCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHH
IAGLDVTLLDMSSERLAFAENELGFSILDGSRGAVPDLVKDATSGEGFDVVFDATGNTRS
HCCCEEEEEECCCCCEEEECCCCCEEEECCCCCCCCHHHHHCCCCCCEEEEEECCCCCHH
VQSAFAHVAHGGTLVLVSVVKDDIVFSDPEFHKREMTLVGSRNALRADFDHVAASIRNGA
HHHHHHHHHCCCEEEEEEEECCCEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCC
VPLAKLVTHRTTLADTPSDLARWAHEKSGLIKAVIEIG
CHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-iditol; NAD+

Specific reaction: L-iditol + NAD+ = L-sorbose + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503 [H]