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The map label for this gene is bepF [H]

Identifier: 13472858

GI number: 13472858

Start: 2635668

End: 2636798

Strand: Direct

Name: bepF [H]

Synonym: mlr3284

Alternate gene names: 13472858

Gene position: 2635668-2636798 (Clockwise)

Preceding gene: 13472856

Following gene: 13472859

Centisome position: 37.46

GC content: 64.37

Gene sequence:

>1131_bases
GTGTCGGCGGCATTCCTGCTCGGTATGGCTTTCAGCAGCACTGCGTTTGCGCAGAACCAGCAGCTACCCGTGGTGACGGC
GGCCAAGCCGGTGGTCCGTGAAATCGTCGAAGACGACGAGTTCGTCGGGCGCTTCGAAGCCGTCGACCAGGTCGCCATCC
GTTCGCGCGTCAGCGGCTATCTGGACAAGGTCAGCTTCCAGGACGGCGCACTGGTCAACAAGGGCGACCTGCTCTTCACC
ATCGACCAGCGTCCTTACCAGGCGGCTTATGATGCCGCCAAATCGCAAGTGGATGTCGCCAAGAGTCTGCTCGAATTCTC
CAAGATGCAGCTGGATCGCGCAGACGAACTCGCCAAGACCGGCAATATCTCAACCGCGACGGTCGACGACCGCCGGCGGG
AATACCTTTCCGCGCAGGCGCAGATGCAGGGCGCGACGGCTGCGCTGACGACGGCTAGCCTGAACATGGAATTCACCGAG
ATCAAGGCGCCCTTGTCCGGCCGCATCGACCGCCGCCTGGTGTCGGTTGGCAACCTTGTGCAGCCGGATTCCACCTTGCT
GACGACCATCGTTACGCGCGATCCGATCGACTTCTATTTCGACGTCGATGAGCGCCTGTATTTCAGCTACGCCCGCGACG
CGAAGGTGCGTGGCGGCAGCATGCAGGAAGGCGCTGGCGGGCTCGACGTCGTGGTTCGCGTCGCCGACCGCGCCGAGGCG
GTATTCAAAGGCAAGCTCGATTTTGCCGAGAACCGGATCGACAACGCGACCGGCACCATGCGGGTGCGTGCAAGGTTTCC
CAACGCCGACGGCATTCTCCAGCCAGGCATGTTCGGGCGCATCAACGTGCCGGGGTCGCTGCCGCATAGCGGCGTGCTGG
TGCCCGACGAGGCGATCGGCGCCGATCAGGATCGCCGCATCGTGTTCCTGGTGGATAACGCCGGGACGGTGTCGGCGAAG
CCCGTGCGCACCGGCCCGCTTCTCGACGGCTACCGGGTGATCCGCGGGGGCCTGACCGGCGACGAGACGATTGTCGTCAA
CGGGCTGATGCACGCCAGGCCCGGCACCAAGGTGAAGGTCGAGATGGTGACGTTGCCACCCAAGGCCGAGACCGCCGGGT
TGCAGCAATGA

Upstream 100 bases:

>100_bases
GCGAGCGAGGACGCAAATGGACACCCCAAAGAATGGGCGATTGCATCGCGACGCAGGCGGACGCCCCCGTCGATCAGCCG
GCTGGCTTGCCCGCGCCGCC

Downstream 100 bases:

>100_bases
GATTCGCACATTTCTTCGTCGACCGTCCGATCTTCGCATCGGTCGTTTCGATCGTCTTGCTGATCCTTGGCGGGATTGCT
TATACCCAGCTGCCGGTCGC

Product: RND efflux membrane fusion protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MSAAFLLGMAFSSTAFAQNQQLPVVTAAKPVVREIVEDDEFVGRFEAVDQVAIRSRVSGYLDKVSFQDGALVNKGDLLFT
IDQRPYQAAYDAAKSQVDVAKSLLEFSKMQLDRADELAKTGNISTATVDDRRREYLSAQAQMQGATAALTTASLNMEFTE
IKAPLSGRIDRRLVSVGNLVQPDSTLLTTIVTRDPIDFYFDVDERLYFSYARDAKVRGGSMQEGAGGLDVVVRVADRAEA
VFKGKLDFAENRIDNATGTMRVRARFPNADGILQPGMFGRINVPGSLPHSGVLVPDEAIGADQDRRIVFLVDNAGTVSAK
PVRTGPLLDGYRVIRGGLTGDETIVVNGLMHARPGTKVKVEMVTLPPKAETAGLQQ

Sequences:

>Translated_376_residues
MSAAFLLGMAFSSTAFAQNQQLPVVTAAKPVVREIVEDDEFVGRFEAVDQVAIRSRVSGYLDKVSFQDGALVNKGDLLFT
IDQRPYQAAYDAAKSQVDVAKSLLEFSKMQLDRADELAKTGNISTATVDDRRREYLSAQAQMQGATAALTTASLNMEFTE
IKAPLSGRIDRRLVSVGNLVQPDSTLLTTIVTRDPIDFYFDVDERLYFSYARDAKVRGGSMQEGAGGLDVVVRVADRAEA
VFKGKLDFAENRIDNATGTMRVRARFPNADGILQPGMFGRINVPGSLPHSGVLVPDEAIGADQDRRIVFLVDNAGTVSAK
PVRTGPLLDGYRVIRGGLTGDETIVVNGLMHARPGTKVKVEMVTLPPKAETAGLQQ
>Mature_375_residues
SAAFLLGMAFSSTAFAQNQQLPVVTAAKPVVREIVEDDEFVGRFEAVDQVAIRSRVSGYLDKVSFQDGALVNKGDLLFTI
DQRPYQAAYDAAKSQVDVAKSLLEFSKMQLDRADELAKTGNISTATVDDRRREYLSAQAQMQGATAALTTASLNMEFTEI
KAPLSGRIDRRLVSVGNLVQPDSTLLTTIVTRDPIDFYFDVDERLYFSYARDAKVRGGSMQEGAGGLDVVVRVADRAEAV
FKGKLDFAENRIDNATGTMRVRARFPNADGILQPGMFGRINVPGSLPHSGVLVPDEAIGADQDRRIVFLVDNAGTVSAKP
VRTGPLLDGYRVIRGGLTGDETIVVNGLMHARPGTKVKVEMVTLPPKAETAGLQQ

Specific function: May contribute to resistance to some drugs, such as deoxycholate, sodium dodecyl sulfate and nalidixic acid, in the absence of BepD and BepE [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=347, Percent_Identity=29.6829971181556, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1789929, Length=324, Percent_Identity=28.7037037037037, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI87082036, Length=337, Percent_Identity=28.486646884273, Blast_Score=127, Evalue=9e-31,
Organism=Escherichia coli, GI1789665, Length=322, Percent_Identity=29.5031055900621, Blast_Score=127, Evalue=9e-31,
Organism=Escherichia coli, GI87081951, Length=183, Percent_Identity=30.6010928961749, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789637, Length=224, Percent_Identity=28.5714285714286, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 40748; Mature: 40617

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAAFLLGMAFSSTAFAQNQQLPVVTAAKPVVREIVEDDEFVGRFEAVDQVAIRSRVSGY
CCCCEEEEHHHCCHHHCCCCCCCEEECCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
LDKVSFQDGALVNKGDLLFTIDQRPYQAAYDAAKSQVDVAKSLLEFSKMQLDRADELAKT
HHHCCCCCCCEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GNISTATVDDRRREYLSAQAQMQGATAALTTASLNMEFTEIKAPLSGRIDRRLVSVGNLV
CCCEEEEECHHHHHHHHHHHHHCCCEEEEEEEECCEEHHEECCCCCCCHHHHHHHHCCCC
QPDSTLLTTIVTRDPIDFYFDVDERLYFSYARDAKVRGGSMQEGAGGLDVVVRVADRAEA
CCCCCEEEEEEECCCCEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEEHHHHHH
VFKGKLDFAENRIDNATGTMRVRARFPNADGILQPGMFGRINVPGSLPHSGVLVPDEAIG
HHCCCCHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCEEEECCCCCCCCCEECCCHHCC
ADQDRRIVFLVDNAGTVSAKPVRTGPLLDGYRVIRGGLTGDETIVVNGLMHARPGTKVKV
CCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHEECCCCCCCEEEEECEEECCCCCEEEE
EMVTLPPKAETAGLQQ
EEEECCCCCCCCCCCC
>Mature Secondary Structure 
SAAFLLGMAFSSTAFAQNQQLPVVTAAKPVVREIVEDDEFVGRFEAVDQVAIRSRVSGY
CCCEEEEHHHCCHHHCCCCCCCEEECCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
LDKVSFQDGALVNKGDLLFTIDQRPYQAAYDAAKSQVDVAKSLLEFSKMQLDRADELAKT
HHHCCCCCCCEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GNISTATVDDRRREYLSAQAQMQGATAALTTASLNMEFTEIKAPLSGRIDRRLVSVGNLV
CCCEEEEECHHHHHHHHHHHHHCCCEEEEEEEECCEEHHEECCCCCCCHHHHHHHHCCCC
QPDSTLLTTIVTRDPIDFYFDVDERLYFSYARDAKVRGGSMQEGAGGLDVVVRVADRAEA
CCCCCEEEEEEECCCCEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEEHHHHHH
VFKGKLDFAENRIDNATGTMRVRARFPNADGILQPGMFGRINVPGSLPHSGVLVPDEAIG
HHCCCCHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCEEEECCCCCCCCCEECCCHHCC
ADQDRRIVFLVDNAGTVSAKPVRTGPLLDGYRVIRGGLTGDETIVVNGLMHARPGTKVKV
CCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHEECCCCCCCEEEEECEEECCCCCEEEE
EMVTLPPKAETAGLQQ
EEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12271122 [H]