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The map label for this gene is cysG [H]

Identifier: 13472817

GI number: 13472817

Start: 2582391

End: 2583842

Strand: Reverse

Name: cysG [H]

Synonym: mll3232

Alternate gene names: 13472817

Gene position: 2583842-2582391 (Counterclockwise)

Preceding gene: 13472820

Following gene: 13472816

Centisome position: 36.72

GC content: 67.7

Gene sequence:

>1452_bases
ATGCCGCAGGCCAATGCCAAGCTCAATGCCTTCCCCGTCTTCGTGCGGGTCGAGGGCGAAGCCGTGGCCATTGTCGGCGG
TGGCGACGAAGCCCTGGCCAAAGCGCGGTTGATTGGACAGTCGAGCGCGGTGCTGCGCATCATCGCCGAAAATGCCGAGC
CGGAGCTGCTTGCCTTTATTGGTCAGTCCGGCGCCAGCCATGTCGCGGCGGCCTACGACGCCTCGCAGCTCGAGGGCGCG
GTCATGGTGTTTGCCGCCAGCGGCGATGAGGCGCTCGACCGCCGTGTCGCGGCCGACGCGCGCGAACTGGGCATTCCGGT
CAATGCCGTTGACCGGCCGGAGCTCTGCGACTTCTTTACCCCGGCGCTGGTCAACCGCGCGCCGGTCGCCATCGCCATCG
GAACCGAAGGCGCCGGTCCGGTGCTGGCCCAGATGTTGCGGGGCCGCATCGATCAGATGCTGTCGCCATCGCTCGGTTCG
CTGGCGACGCTCGCCGCCACGCTGCGTGGGGCAGCCGAGAAATTGCTGCCGAAAGGCAATGCCCGCCGCCGCTTCTGGAA
CAGTTTTTTCGAAGGTGCACCTGCCCATGCCGTGGAAGCCGGCCAGCTGTCGCAGGCGCATGACGCCGCCGTCGATCTGC
TGCTGTCGAACGCGCCGGCTTCGGGTCACATCGCCCTGGTCGGTGCCGGTCCGGGCGCCGAGGATCTGCTGACGTTGCGG
GCGCATCGCCTGCTGATGGAGGCCGATGTCATCGTCCATGATGCGCTGGTGCCGGAGGCGGTCGTCGCCATGGGCCGCCG
CGATGCCGAGCGCCTTCCGGTCGGCAAACGCAAGGGCTGCCACACCAAGAGCCAGGCCGAGATCAACGCGCTGCTGGTCG
AGCTCGGCCGCCAGGGCAAGCGCGTGGTGCGGCTGAAGTCGGGCGATCCGCTGGTGTTCGGTCGCGCCGGCGAGGAGATG
GCGGCACTGCGCGATGCCGGCATCGCCTATGAGGTGGTTCCGGGCGTCACCGCGGCCTTTGCCGCCGCCGCCGATTTCGA
ATTGCCGCTGACCTTGCGTGGCGTCTCCTCGTCGATGGTGTTCACCACCGGCCATGACCTCAAGGGCAATTCATTGCCCG
ACTGGGCCAAGCTTGCGATTTCTGGCGCCACGGTCGCCGTCTATATGGGCCGCTCGGTGGCGGCCGAAGTCGCCGGCCGG
CTGATCGAGGCCGGCCTGTCGCCGGACACGGCGGTGGCCGTGGTCGAGAATGCCAGCCTGGCCAACCGTCGCCGCTTTCA
CGGAACGTTGGCCGACCTGCCGTCGCTGGAAGCGCGTGGCGATCTTTCCGGGCCGGTCATGACGATCATCGGCGATGCTG
TTGCCGGCGCCAATTTTGAACGGTCCGAGCCGCTCGCCGCACATAGGAATGAAGGCGCGGCCAAAGTTGCCGCAGAGAGA
GTTCAGCCATGA

Upstream 100 bases:

>100_bases
AAAGCGATTGCCTGCCACCACAGGATGCGCGAGTGCTTTGCTTCTGGTTTTTCGCGCCCGAGCGTAAAACTGCGACACTG
TTCCCCGGATTGCTTTTCCT

Downstream 100 bases:

>100_bases
AGATACTGACCGCCAACCGCCTGACCGACGGCATCGCCGTCTGGTACGCCGATGGCGGCTGGGCCGAGACGGTGGGCCGG
GCCGACCTTGCCCATGACAA

Product: siroheme synthetase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Precorrin-2 dehydrogenase; Sirohydrochlorin ferrochelatase [H]

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MPQANAKLNAFPVFVRVEGEAVAIVGGGDEALAKARLIGQSSAVLRIIAENAEPELLAFIGQSGASHVAAAYDASQLEGA
VMVFAASGDEALDRRVAADARELGIPVNAVDRPELCDFFTPALVNRAPVAIAIGTEGAGPVLAQMLRGRIDQMLSPSLGS
LATLAATLRGAAEKLLPKGNARRRFWNSFFEGAPAHAVEAGQLSQAHDAAVDLLLSNAPASGHIALVGAGPGAEDLLTLR
AHRLLMEADVIVHDALVPEAVVAMGRRDAERLPVGKRKGCHTKSQAEINALLVELGRQGKRVVRLKSGDPLVFGRAGEEM
AALRDAGIAYEVVPGVTAAFAAAADFELPLTLRGVSSSMVFTTGHDLKGNSLPDWAKLAISGATVAVYMGRSVAAEVAGR
LIEAGLSPDTAVAVVENASLANRRRFHGTLADLPSLEARGDLSGPVMTIIGDAVAGANFERSEPLAAHRNEGAAKVAAER
VQP

Sequences:

>Translated_483_residues
MPQANAKLNAFPVFVRVEGEAVAIVGGGDEALAKARLIGQSSAVLRIIAENAEPELLAFIGQSGASHVAAAYDASQLEGA
VMVFAASGDEALDRRVAADARELGIPVNAVDRPELCDFFTPALVNRAPVAIAIGTEGAGPVLAQMLRGRIDQMLSPSLGS
LATLAATLRGAAEKLLPKGNARRRFWNSFFEGAPAHAVEAGQLSQAHDAAVDLLLSNAPASGHIALVGAGPGAEDLLTLR
AHRLLMEADVIVHDALVPEAVVAMGRRDAERLPVGKRKGCHTKSQAEINALLVELGRQGKRVVRLKSGDPLVFGRAGEEM
AALRDAGIAYEVVPGVTAAFAAAADFELPLTLRGVSSSMVFTTGHDLKGNSLPDWAKLAISGATVAVYMGRSVAAEVAGR
LIEAGLSPDTAVAVVENASLANRRRFHGTLADLPSLEARGDLSGPVMTIIGDAVAGANFERSEPLAAHRNEGAAKVAAER
VQP
>Mature_482_residues
PQANAKLNAFPVFVRVEGEAVAIVGGGDEALAKARLIGQSSAVLRIIAENAEPELLAFIGQSGASHVAAAYDASQLEGAV
MVFAASGDEALDRRVAADARELGIPVNAVDRPELCDFFTPALVNRAPVAIAIGTEGAGPVLAQMLRGRIDQMLSPSLGSL
ATLAATLRGAAEKLLPKGNARRRFWNSFFEGAPAHAVEAGQLSQAHDAAVDLLLSNAPASGHIALVGAGPGAEDLLTLRA
HRLLMEADVIVHDALVPEAVVAMGRRDAERLPVGKRKGCHTKSQAEINALLVELGRQGKRVVRLKSGDPLVFGRAGEEMA
ALRDAGIAYEVVPGVTAAFAAAADFELPLTLRGVSSSMVFTTGHDLKGNSLPDWAKLAISGATVAVYMGRSVAAEVAGRL
IEAGLSPDTAVAVVENASLANRRRFHGTLADLPSLEARGDLSGPVMTIIGDAVAGANFERSEPLAAHRNEGAAKVAAERV
QP

Specific function: Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into si

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=449, Percent_Identity=37.8619153674833, Blast_Score=287, Evalue=1e-78,
Organism=Saccharomyces cerevisiae, GI6322922, Length=233, Percent_Identity=31.7596566523605, Blast_Score=107, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR016040
- InterPro:   IPR019478
- InterPro:   IPR006367
- InterPro:   IPR003043 [H]

Pfam domain/function: PF10414 CysG_dimeriser; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =1.3.1.76; =4.99.1.4 [H]

Molecular weight: Translated: 50041; Mature: 49910

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQANAKLNAFPVFVRVEGEAVAIVGGGDEALAKARLIGQSSAVLRIIAENAEPELLAFI
CCCCCCCCEEEEEEEEECCCEEEEEECCHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEE
GQSGASHVAAAYDASQLEGAVMVFAASGDEALDRRVAADARELGIPVNAVDRPELCDFFT
CCCCCCCEEECCCHHHCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
PALVNRAPVAIAIGTEGAGPVLAQMLRGRIDQMLSPSLGSLATLAATLRGAAEKLLPKGN
HHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCC
ARRRFWNSFFEGAPAHAVEAGQLSQAHDAAVDLLLSNAPASGHIALVGAGPGAEDLLTLR
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHH
AHRLLMEADVIVHDALVPEAVVAMGRRDAERLPVGKRKGCHTKSQAEINALLVELGRQGK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
RVVRLKSGDPLVFGRAGEEMAALRDAGIAYEVVPGVTAAFAAAADFELPLTLRGVSSSMV
EEEEEECCCCEEEECCCHHHHHHHHCCCEEEECCCHHHHHHHHCCCCCCEEEECCCCCEE
FTTGHDLKGNSLPDWAKLAISGATVAVYMGRSVAAEVAGRLIEAGLSPDTAVAVVENASL
EEECCCCCCCCCCHHHHHEECCCEEEEEECCHHHHHHHHHHHHCCCCCCCEEEEEECCCH
ANRRRFHGTLADLPSLEARGDLSGPVMTIIGDAVAGANFERSEPLAAHRNEGAAKVAAER
HHHHHHCCHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHCCCCHHHHHHHH
VQP
CCC
>Mature Secondary Structure 
PQANAKLNAFPVFVRVEGEAVAIVGGGDEALAKARLIGQSSAVLRIIAENAEPELLAFI
CCCCCCCEEEEEEEEECCCEEEEEECCHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEE
GQSGASHVAAAYDASQLEGAVMVFAASGDEALDRRVAADARELGIPVNAVDRPELCDFFT
CCCCCCCEEECCCHHHCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
PALVNRAPVAIAIGTEGAGPVLAQMLRGRIDQMLSPSLGSLATLAATLRGAAEKLLPKGN
HHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCC
ARRRFWNSFFEGAPAHAVEAGQLSQAHDAAVDLLLSNAPASGHIALVGAGPGAEDLLTLR
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHH
AHRLLMEADVIVHDALVPEAVVAMGRRDAERLPVGKRKGCHTKSQAEINALLVELGRQGK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
RVVRLKSGDPLVFGRAGEEMAALRDAGIAYEVVPGVTAAFAAAADFELPLTLRGVSSSMV
EEEEEECCCCEEEECCCHHHHHHHHCCCEEEECCCHHHHHHHHCCCCCCEEEECCCCCEE
FTTGHDLKGNSLPDWAKLAISGATVAVYMGRSVAAEVAGRLIEAGLSPDTAVAVVENASL
EEECCCCCCCCCCHHHHHEECCCEEEEEECCHHHHHHHHHHHHCCCCCCCEEEEEECCCH
ANRRRFHGTLADLPSLEARGDLSGPVMTIIGDAVAGANFERSEPLAAHRNEGAAKVAAER
HHHHHHCCHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHCCCCHHHHHHHH
VQP
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA