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The map label for this gene is alkJ [H]

Identifier: 13472812

GI number: 13472812

Start: 2577301

End: 2578908

Strand: Reverse

Name: alkJ [H]

Synonym: mll3225

Alternate gene names: 13472812

Gene position: 2578908-2577301 (Counterclockwise)

Preceding gene: 13472813

Following gene: 13472811

Centisome position: 36.65

GC content: 64.43

Gene sequence:

>1608_bases
ATGGATTTCGACTATGTCATCGCCGGCGGCGGGTCCGCCGGCTGCACGCTTGCCGCGCGTCTGTCCGAAGATCCGTCGAA
AACGGTCTGCCTGATCGAAGCCGGCGGCGCGGGCAGCAACCTGCTTATCCGCGCGCCGGCAGGCATCATCGCACTGTTGC
CGGGCCGGCCGAAAATCAACAACTGGGCGTTCGAGACGGTGCCGCAGCAAGGTCTTGGCGGCCGCAAGGGCTACCAGCCG
CGCGGCAAGGCGCTGGGCGGATCCAGCGCCATCAATGCCATGCTCTATACGCGCGGGCACCGCGGCGACTATGACGAATG
GGCCGACCTCGGCTGCGACGGCTGGTCATGGGACGAGGTGCTGCCCTATTTCCGGCGGGCGGAAGGCAATCAACGCGGCG
CTGATGCCCTGCATGGCGGTGACGGGCCGCTGCGGGTTGCCGAGCAGCAAGAGCCACGCCCGCTCTCCCGGGCCTTTGTC
GAGGCCTGCGGCGAAAACCAGATCCGCCGCAACGATGATTTCAACGGCGTCGAACAGGAAGGTGCCGGCCTTTATCAGGT
GACGCAATTCTGGGGCGAACGCCGCAATGGCGAGCGCTGCTCGGTGGCCGCCGCCTATCTGCAACCGGCCATGAACAGGT
CGAACCTTACCGTCATCACCGGAGCGCACGCCACGGCGGTCATCCTCGATGGCAAGCGCGCCACCGGCGTGCGGTATCGC
GCGGGGAAGGACGAAGCCGTCGCGAAAGCCAGGCGTGAGGTGATCGTCTGTGGTGGCGCCTTCGGTTCGCCTCAACTCCT
GTTGCTGTCGGGCATTGGCCCGGCTGCCGAGCTTGCCATGCATGGCATTCCGGTCATTCATGAATTGCCCGGTGTTGGCA
AGAACCTGCAAGACCATCTCGATTTCATCATGGGCTGGACGTCCAAGGATGCCGACATGATGGGCATCGGCCTGCGCGGC
CTGCCTGGCCTGCTGCGGCACATGCTGCGCTGGCGCAAAGATGGCGGCGGCATGATCGCCACGCCCTATGCCGAAGGTGG
TGCCTTCCTCAAATCCGATCCGGCGATAGAACGGCCCGACCTGCAACTGCATTTCTGCATCGCGATCGTGGATGATCATG
GCCGCAAGCTGCATATGGGATATGGCTTTTCCTGCCATGTCTGCGTGCTGCGGCCGCATTCGCGCGGCGAGGTCGGGCTG
TCGACTCATGATCCCATGGCACCGCCGCGCATCGATCCTCGCTTTCTTTCGGACGAACGCGACGCCGAGCTTTTGCTCAA
GGGGGTCAGGATGATGCGCGGCATCCTGGAGGCGCCGGCGCTGGCCAGGTATCGCCACAAGGAAATCTACACCGCCGGTG
TTTCGAGCGACGCGGATTTGATGTCCCACATCCGTGCCCGCGCCGACACGATCTATCACCCGGCCGGCACCTGCAAGATG
GGCGTCGACGAGCTGGCGGTGGTCGATCCGCAATTGCGGGTGCACGGGCTTGAAGCCTTGCGGGTTGTTGACGCCTCTGT
GATGCCGACCCTGATCGGCGGCAACACCAACGCTCCGACCATCATGATTGCCGAGAAGGCGGCGGATATGATCAAGGCAG
CCGCCTGA

Upstream 100 bases:

>100_bases
GCTGACGCTGCGGTAATTCTACCATCTTTACATAGGCCGTAATCGAGCCTTCTCTTGGTCATCCGGACGGAAACATCCGC
CGGGTGCGAGGGAGGAAGTC

Downstream 100 bases:

>100_bases
CTGCCGCTCTGGTCGAACCAGCGAAGCTATTTTCGTCTGCCGGGAAGCGCTCTCGGTCGAGAAGGTGCAAATCTGGTCAT
ACCAATTTGGCGATTTGCGC

Product: dehydrogenase (polyethylene glycol dehydrogenase, alcohol dehydrogenase, L-sorbose dehydrogenase)

Products: Betaine Aldehyde; Reduced Acceptor. [C]

Alternate protein names: NA

Number of amino acids: Translated: 535; Mature: 535

Protein sequence:

>535_residues
MDFDYVIAGGGSAGCTLAARLSEDPSKTVCLIEAGGAGSNLLIRAPAGIIALLPGRPKINNWAFETVPQQGLGGRKGYQP
RGKALGGSSAINAMLYTRGHRGDYDEWADLGCDGWSWDEVLPYFRRAEGNQRGADALHGGDGPLRVAEQQEPRPLSRAFV
EACGENQIRRNDDFNGVEQEGAGLYQVTQFWGERRNGERCSVAAAYLQPAMNRSNLTVITGAHATAVILDGKRATGVRYR
AGKDEAVAKARREVIVCGGAFGSPQLLLLSGIGPAAELAMHGIPVIHELPGVGKNLQDHLDFIMGWTSKDADMMGIGLRG
LPGLLRHMLRWRKDGGGMIATPYAEGGAFLKSDPAIERPDLQLHFCIAIVDDHGRKLHMGYGFSCHVCVLRPHSRGEVGL
STHDPMAPPRIDPRFLSDERDAELLLKGVRMMRGILEAPALARYRHKEIYTAGVSSDADLMSHIRARADTIYHPAGTCKM
GVDELAVVDPQLRVHGLEALRVVDASVMPTLIGGNTNAPTIMIAEKAADMIKAAA

Sequences:

>Translated_535_residues
MDFDYVIAGGGSAGCTLAARLSEDPSKTVCLIEAGGAGSNLLIRAPAGIIALLPGRPKINNWAFETVPQQGLGGRKGYQP
RGKALGGSSAINAMLYTRGHRGDYDEWADLGCDGWSWDEVLPYFRRAEGNQRGADALHGGDGPLRVAEQQEPRPLSRAFV
EACGENQIRRNDDFNGVEQEGAGLYQVTQFWGERRNGERCSVAAAYLQPAMNRSNLTVITGAHATAVILDGKRATGVRYR
AGKDEAVAKARREVIVCGGAFGSPQLLLLSGIGPAAELAMHGIPVIHELPGVGKNLQDHLDFIMGWTSKDADMMGIGLRG
LPGLLRHMLRWRKDGGGMIATPYAEGGAFLKSDPAIERPDLQLHFCIAIVDDHGRKLHMGYGFSCHVCVLRPHSRGEVGL
STHDPMAPPRIDPRFLSDERDAELLLKGVRMMRGILEAPALARYRHKEIYTAGVSSDADLMSHIRARADTIYHPAGTCKM
GVDELAVVDPQLRVHGLEALRVVDASVMPTLIGGNTNAPTIMIAEKAADMIKAAA
>Mature_535_residues
MDFDYVIAGGGSAGCTLAARLSEDPSKTVCLIEAGGAGSNLLIRAPAGIIALLPGRPKINNWAFETVPQQGLGGRKGYQP
RGKALGGSSAINAMLYTRGHRGDYDEWADLGCDGWSWDEVLPYFRRAEGNQRGADALHGGDGPLRVAEQQEPRPLSRAFV
EACGENQIRRNDDFNGVEQEGAGLYQVTQFWGERRNGERCSVAAAYLQPAMNRSNLTVITGAHATAVILDGKRATGVRYR
AGKDEAVAKARREVIVCGGAFGSPQLLLLSGIGPAAELAMHGIPVIHELPGVGKNLQDHLDFIMGWTSKDADMMGIGLRG
LPGLLRHMLRWRKDGGGMIATPYAEGGAFLKSDPAIERPDLQLHFCIAIVDDHGRKLHMGYGFSCHVCVLRPHSRGEVGL
STHDPMAPPRIDPRFLSDERDAELLLKGVRMMRGILEAPALARYRHKEIYTAGVSSDADLMSHIRARADTIYHPAGTCKM
GVDELAVVDPQLRVHGLEALRVVDASVMPTLIGGNTNAPTIMIAEKAADMIKAAA

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=544, Percent_Identity=44.4852941176471, Blast_Score=414, Evalue=1e-115,
Organism=Escherichia coli, GI1786503, Length=538, Percent_Identity=42.5650557620818, Blast_Score=394, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17532301, Length=550, Percent_Identity=38.1818181818182, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI45549471, Length=579, Percent_Identity=38.3419689119171, Blast_Score=339, Evalue=3e-93,
Organism=Drosophila melanogaster, GI45551458, Length=580, Percent_Identity=38.1034482758621, Blast_Score=339, Evalue=3e-93,
Organism=Drosophila melanogaster, GI24642042, Length=583, Percent_Identity=38.0789022298456, Blast_Score=320, Evalue=2e-87,
Organism=Drosophila melanogaster, GI24642048, Length=581, Percent_Identity=35.9724612736661, Blast_Score=312, Evalue=4e-85,
Organism=Drosophila melanogaster, GI24642055, Length=578, Percent_Identity=34.4290657439446, Blast_Score=309, Evalue=4e-84,
Organism=Drosophila melanogaster, GI24642059, Length=573, Percent_Identity=35.7766143106457, Blast_Score=306, Evalue=3e-83,
Organism=Drosophila melanogaster, GI24642035, Length=578, Percent_Identity=35.9861591695502, Blast_Score=305, Evalue=4e-83,
Organism=Drosophila melanogaster, GI24650267, Length=575, Percent_Identity=36, Blast_Score=303, Evalue=1e-82,
Organism=Drosophila melanogaster, GI24642037, Length=581, Percent_Identity=36.4888123924269, Blast_Score=291, Evalue=6e-79,
Organism=Drosophila melanogaster, GI17137792, Length=572, Percent_Identity=34.6153846153846, Blast_Score=284, Evalue=8e-77,
Organism=Drosophila melanogaster, GI24642039, Length=573, Percent_Identity=35.9511343804538, Blast_Score=281, Evalue=6e-76,
Organism=Drosophila melanogaster, GI18859995, Length=569, Percent_Identity=35.676625659051, Blast_Score=275, Evalue=4e-74,
Organism=Drosophila melanogaster, GI18859993, Length=579, Percent_Identity=33.5060449050086, Blast_Score=255, Evalue=6e-68,
Organism=Drosophila melanogaster, GI24642051, Length=573, Percent_Identity=32.6352530541012, Blast_Score=243, Evalue=2e-64,
Organism=Drosophila melanogaster, GI24645930, Length=586, Percent_Identity=32.4232081911263, Blast_Score=189, Evalue=3e-48,
Organism=Drosophila melanogaster, GI24642057, Length=592, Percent_Identity=29.7297297297297, Blast_Score=152, Evalue=8e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1 [C]

Molecular weight: Translated: 57586; Mature: 57586

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS00623 GMC_OXRED_1 ; PS00624 GMC_OXRED_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFDYVIAGGGSAGCTLAARLSEDPSKTVCLIEAGGAGSNLLIRAPAGIIALLPGRPKIN
CCCEEEEECCCCCCCEEEEECCCCCCCEEEEEEECCCCCCEEEECCCCEEEEECCCCCCC
NWAFETVPQQGLGGRKGYQPRGKALGGSSAINAMLYTRGHRGDYDEWADLGCDGWSWDEV
CCEEECCCCCCCCCCCCCCCCCCCCCCHHHHEEEEEECCCCCCCHHHHHCCCCCCCHHHH
LPYFRRAEGNQRGADALHGGDGPLRVAEQQEPRPLSRAFVEACGENQIRRNDDFNGVEQE
HHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHC
GAGLYQVTQFWGERRNGERCSVAAAYLQPAMNRSNLTVITGAHATAVILDGKRATGVRYR
CCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCEEEEEECCCCCCCEEEE
AGKDEAVAKARREVIVCGGAFGSPQLLLLSGIGPAAELAMHGIPVIHELPGVGKNLQDHL
CCCHHHHHHHCCEEEEEECCCCCCCEEEEECCCHHHHHHHCCCCHHHHCCCCCCCHHHHH
DFIMGWTSKDADMMGIGLRGLPGLLRHMLRWRKDGGGMIATPYAEGGAFLKSDPAIERPD
HHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCEECCCCCCCCCC
LQLHFCIAIVDDHGRKLHMGYGFSCHVCVLRPHSRGEVGLSTHDPMAPPRIDPRFLSDER
CEEEEEEEEEECCCCEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DAELLLKGVRMMRGILEAPALARYRHKEIYTAGVSSDADLMSHIRARADTIYHPAGTCKM
CHHHHHHHHHHHHHHHHCCHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHEECCCCCCCC
GVDELAVVDPQLRVHGLEALRVVDASVMPTLIGGNTNAPTIMIAEKAADMIKAAA
CCCHHEEECCCHHHHHHHHHHHHHHHHCCEEECCCCCCCEEEEEHHHHHHHHHCC
>Mature Secondary Structure
MDFDYVIAGGGSAGCTLAARLSEDPSKTVCLIEAGGAGSNLLIRAPAGIIALLPGRPKIN
CCCEEEEECCCCCCCEEEEECCCCCCCEEEEEEECCCCCCEEEECCCCEEEEECCCCCCC
NWAFETVPQQGLGGRKGYQPRGKALGGSSAINAMLYTRGHRGDYDEWADLGCDGWSWDEV
CCEEECCCCCCCCCCCCCCCCCCCCCCHHHHEEEEEECCCCCCCHHHHHCCCCCCCHHHH
LPYFRRAEGNQRGADALHGGDGPLRVAEQQEPRPLSRAFVEACGENQIRRNDDFNGVEQE
HHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHC
GAGLYQVTQFWGERRNGERCSVAAAYLQPAMNRSNLTVITGAHATAVILDGKRATGVRYR
CCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCEEEEEECCCCCCCEEEE
AGKDEAVAKARREVIVCGGAFGSPQLLLLSGIGPAAELAMHGIPVIHELPGVGKNLQDHL
CCCHHHHHHHCCEEEEEECCCCCCCEEEEECCCHHHHHHHCCCCHHHHCCCCCCCHHHHH
DFIMGWTSKDADMMGIGLRGLPGLLRHMLRWRKDGGGMIATPYAEGGAFLKSDPAIERPD
HHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCEECCCCCCCCCC
LQLHFCIAIVDDHGRKLHMGYGFSCHVCVLRPHSRGEVGLSTHDPMAPPRIDPRFLSDER
CEEEEEEEEEECCCCEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DAELLLKGVRMMRGILEAPALARYRHKEIYTAGVSSDADLMSHIRARADTIYHPAGTCKM
CHHHHHHHHHHHHHHHHCCHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHEECCCCCCCC
GVDELAVVDPQLRVHGLEALRVVDASVMPTLIGGNTNAPTIMIAEKAADMIKAAA
CCCHHEEECCCHHHHHHHHHHHHHHHHCCEEECCCCCCCEEEEEHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: Choline; Acceptor [C]

Specific reaction: Choline + Acceptor = Betaine Aldehyde + Reduced Acceptor. [C]

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11390693 [H]