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The map label for this gene is puuB [H]

Identifier: 13472601

GI number: 13472601

Start: 2369816

End: 2371201

Strand: Reverse

Name: puuB [H]

Synonym: mll2947

Alternate gene names: 13472601

Gene position: 2371201-2369816 (Counterclockwise)

Preceding gene: 13472602

Following gene: 13472600

Centisome position: 33.7

GC content: 67.1

Gene sequence:

>1386_bases
ATGCAAGCCAAGCGCAACGGCGACATATCGTTCTGGTATGCGGATCTGGGAGGGCTCCCCGCGCCCCGGCCACCGTTGCC
CGGCGATATCGAGGCCGATGTCGCGATCGTTGGCGCCGGCTATACCGGCCTGTGGACCGCCTATTATCTGAAGAAGGCGA
GACCCTCGCTGCGCATCGCGGTGATCGAGCGCGAGTTCGCCGGTTTCGGCGCTTCTGGCCGCAATGGCGGCTGGCTGTCG
GGCGGCTTCTCCTGGTCGCGCGAAAAATATCTCAAGACCTCGACCCGGCAAGGGGTGAGCGCTATGCAGGCGGCCATGGC
GGGCTGCGTCGACGAGGTGATCCGGGTGGCGAGCGCGGAAGGCATCGATGCCGATATTCGCCGCGTCGACAATCTGACGG
TGGCTACCAACCCCGCGCAGCTCGAACGCGCCCGCGAGGAGTGCGAAACGATCCGCGCCTGGGATGTCGATCCGACCCGT
GTCGAAATGCTGGATCAAGCGGCGACGCGGGCGCGCATCAACATCCGCAATGTCATGGGTGGCTTCGTCGTCCACGGCCA
GGCGCGGGTGCAGCCGGCCAGGCTGGTGCGCGGGCTTGCCGCCGCCGTCGAGCGGCTGGGCGTGCCGATCTACGAACAGA
CGACAGTGACAGCCATAGCCAAGGGCAGCGTGACGACGAACCGCGGCACTGTGCGGGTGGAAACCATCATCCGCGCGACA
GAGGGGTTTACATCAGGCATTCCTGGCGAGGAGCGGACCTGGCTAGCGCTCAACAGCGCGCAGATCGTCACCGAGCCACT
GTCTGAGGAGCTCTGGCGCCAGATCGGCTGGGAGGGCCATGAGCTTCTCGGCAACGCCGCCCACGCCTATTGCTATGCGC
AGCGCACGCGCGAGGGGCGCATCACCATGGGCGGGCGCGGCGTGCCCTATCGCTATGGCTCGCGCACCGACGTCAACGGG
CAGACGCAGCAGGCCACGATCGACCAACTGCATCAGATCCTGACGACGCTGTTGCCGCAGACGGCGAACTGCCGCATCGA
GCACGCCTGGTGCGGCGTGCTCGGGGTGCCGCGCGACTGGTGCACGACCGTCGGCCTCGATCCCGCGACCCGCATCGGCT
GGGCCGGCGGCTATGTCGGGCTTGGTGTGTCGAGCTCCAATCTTTCCGGGCGCACGCTGGCCGACCTGATCCTCGGCCAG
GAGACGGAGCTGACGAAGCTGCCCTGGGTCAACCGCAAGGTCAGGCCATGGGAGCCGGAGCCGTTCCGCTGGCTCGGCGT
GCATTCGATGTACCAGCTTTATCGCATCGCCGACGGGCGCGAAGCCGCTGGGCTTGCGCATACGTCGAGACTGGCCGCGC
TCGCCGACAGCATTACGGGCCACTGA

Upstream 100 bases:

>100_bases
AGGTGGAGCTCTGCTTCGACGGTGCCGACGCCAAGCTTCTGGCCGACGACAGCTCAGCCGACGCGACGCTAACCTGATCC
ATCAATCTGAAAGTCATCCC

Downstream 100 bases:

>100_bases
TTGATGGAGACCAGTTTGATTGACCTTTCGACCTTCCACGATCTCACCAAGGCCGACATCGGCGCGTTCTCACCCAAGCC
CACCTCCATCGAGGGGGATC

Product: hypothetical protein

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MQAKRNGDISFWYADLGGLPAPRPPLPGDIEADVAIVGAGYTGLWTAYYLKKARPSLRIAVIEREFAGFGASGRNGGWLS
GGFSWSREKYLKTSTRQGVSAMQAAMAGCVDEVIRVASAEGIDADIRRVDNLTVATNPAQLERAREECETIRAWDVDPTR
VEMLDQAATRARINIRNVMGGFVVHGQARVQPARLVRGLAAAVERLGVPIYEQTTVTAIAKGSVTTNRGTVRVETIIRAT
EGFTSGIPGEERTWLALNSAQIVTEPLSEELWRQIGWEGHELLGNAAHAYCYAQRTREGRITMGGRGVPYRYGSRTDVNG
QTQQATIDQLHQILTTLLPQTANCRIEHAWCGVLGVPRDWCTTVGLDPATRIGWAGGYVGLGVSSSNLSGRTLADLILGQ
ETELTKLPWVNRKVRPWEPEPFRWLGVHSMYQLYRIADGREAAGLAHTSRLAALADSITGH

Sequences:

>Translated_461_residues
MQAKRNGDISFWYADLGGLPAPRPPLPGDIEADVAIVGAGYTGLWTAYYLKKARPSLRIAVIEREFAGFGASGRNGGWLS
GGFSWSREKYLKTSTRQGVSAMQAAMAGCVDEVIRVASAEGIDADIRRVDNLTVATNPAQLERAREECETIRAWDVDPTR
VEMLDQAATRARINIRNVMGGFVVHGQARVQPARLVRGLAAAVERLGVPIYEQTTVTAIAKGSVTTNRGTVRVETIIRAT
EGFTSGIPGEERTWLALNSAQIVTEPLSEELWRQIGWEGHELLGNAAHAYCYAQRTREGRITMGGRGVPYRYGSRTDVNG
QTQQATIDQLHQILTTLLPQTANCRIEHAWCGVLGVPRDWCTTVGLDPATRIGWAGGYVGLGVSSSNLSGRTLADLILGQ
ETELTKLPWVNRKVRPWEPEPFRWLGVHSMYQLYRIADGREAAGLAHTSRLAALADSITGH
>Mature_461_residues
MQAKRNGDISFWYADLGGLPAPRPPLPGDIEADVAIVGAGYTGLWTAYYLKKARPSLRIAVIEREFAGFGASGRNGGWLS
GGFSWSREKYLKTSTRQGVSAMQAAMAGCVDEVIRVASAEGIDADIRRVDNLTVATNPAQLERAREECETIRAWDVDPTR
VEMLDQAATRARINIRNVMGGFVVHGQARVQPARLVRGLAAAVERLGVPIYEQTTVTAIAKGSVTTNRGTVRVETIIRAT
EGFTSGIPGEERTWLALNSAQIVTEPLSEELWRQIGWEGHELLGNAAHAYCYAQRTREGRITMGGRGVPYRYGSRTDVNG
QTQQATIDQLHQILTTLLPQTANCRIEHAWCGVLGVPRDWCTTVGLDPATRIGWAGGYVGLGVSSSNLSGRTLADLILGQ
ETELTKLPWVNRKVRPWEPEPFRWLGVHSMYQLYRIADGREAAGLAHTSRLAALADSITGH

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=400, Percent_Identity=29.75, Blast_Score=112, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 50360; Mature: 50360

Theoretical pI: Translated: 8.55; Mature: 8.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAKRNGDISFWYADLGGLPAPRPPLPGDIEADVAIVGAGYTGLWTAYYLKKARPSLRIA
CCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCEEEE
VIEREFAGFGASGRNGGWLSGGFSWSREKYLKTSTRQGVSAMQAAMAGCVDEVIRVASAE
EEEEHHHCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GIDADIRRVDNLTVATNPAQLERAREECETIRAWDVDPTRVEMLDQAATRARINIRNVMG
CCCHHHHHHCCCEEECCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEHHHHHC
GFVVHGQARVQPARLVRGLAAAVERLGVPIYEQTTVTAIAKGSVTTNRGTVRVETIIRAT
CEEEECCCCCCHHHHHHHHHHHHHHHCCCEECCCEEEEEECCCEECCCCCEEEEEEHHHH
EGFTSGIPGEERTWLALNSAQIVTEPLSEELWRQIGWEGHELLGNAAHAYCYAQRTREGR
CCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHCCCCE
ITMGGRGVPYRYGSRTDVNGQTQQATIDQLHQILTTLLPQTANCRIEHAWCGVLGVPRDW
EEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHH
CTTVGLDPATRIGWAGGYVGLGVSSSNLSGRTLADLILGQETELTKLPWVNRKVRPWEPE
HHHCCCCCHHHCCCCCCEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
PFRWLGVHSMYQLYRIADGREAAGLAHTSRLAALADSITGH
CCEEECHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQAKRNGDISFWYADLGGLPAPRPPLPGDIEADVAIVGAGYTGLWTAYYLKKARPSLRIA
CCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCEEEE
VIEREFAGFGASGRNGGWLSGGFSWSREKYLKTSTRQGVSAMQAAMAGCVDEVIRVASAE
EEEEHHHCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GIDADIRRVDNLTVATNPAQLERAREECETIRAWDVDPTRVEMLDQAATRARINIRNVMG
CCCHHHHHHCCCEEECCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEHHHHHC
GFVVHGQARVQPARLVRGLAAAVERLGVPIYEQTTVTAIAKGSVTTNRGTVRVETIIRAT
CEEEECCCCCCHHHHHHHHHHHHHHHCCCEECCCEEEEEECCCEECCCCCEEEEEEHHHH
EGFTSGIPGEERTWLALNSAQIVTEPLSEELWRQIGWEGHELLGNAAHAYCYAQRTREGR
CCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHCCCCE
ITMGGRGVPYRYGSRTDVNGQTQQATIDQLHQILTTLLPQTANCRIEHAWCGVLGVPRDW
EEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHH
CTTVGLDPATRIGWAGGYVGLGVSSSNLSGRTLADLILGQETELTKLPWVNRKVRPWEPE
HHHCCCCCHHHCCCCCCEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
PFRWLGVHSMYQLYRIADGREAAGLAHTSRLAALADSITGH
CCEEECHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]