Click here to switch to the map view.

The map label for this gene is fliF [H]

Identifier: 13472585

GI number: 13472585

Start: 2354339

End: 2355988

Strand: Direct

Name: fliF [H]

Synonym: mlr2928

Alternate gene names: 13472585

Gene position: 2354339-2355988 (Clockwise)

Preceding gene: 13472584

Following gene: 13472586

Centisome position: 33.46

GC content: 65.7

Gene sequence:

>1650_bases
GTGCCGGAACAGATCCAGAGCATCATCTCGAATCTCCGCGGTTTTGGTGTGAAGCGCCTCGCCATGCTGGCGGGCATCGC
CGTTCTGGTGATGGGCGTCATCGGCATCGCCTCGGTCTACCTCAACCGCCCGGCCTACGACACGCTCTACGTCGGGCTCG
ACCGAGCCGATGTGAACCAGATCGGCCTGGTGCTGGGTGAGGCCGGCATCGGCTTCGATGTCGGCTCCGACGGAACCTCG
GTTCTGGTGCCGGCCGGCACAACGGCGCAGGCGCGCATGCTGCTTGCCGAAAAGGGTCTGCCGACCAGCGCCAATGCCGG
CTACGAGCTGTTCGACAATGTCGGCGCGATGGGCCTGACCTCGTTCATGCAGCAGATCACCCGCGTGCGTGCGCTCGAAG
GCGAGATCGCCCGCACCATCCAGTCCATATCGGGCATCAAGGCGGCACGCGTCCACATCGTCATGTCCGAGCGCGCCAAT
TTCCGCCGCGACGAACAGCAGCCCTCGGCATCGGTCGTCATCCGCTATGCCGGCATCGACGCCGAGAAAAGCGCCCAATC
GATCCGCCATCTCGTCGCCGCCGCCGTGCCTGGCCTGTCGGCCGACAAGGTGACGGTGCTCGATTCCAACGGCAACCTGC
TCGCCGCCGGCGATGATCCGTCCAACACCAGCGCGGCCCGCACGCTCGGCGTCGAGCAGACCGTGGAGGCGCAGATCGGC
GACAACATCCGCCGCGCGCTCACCCCCTATCTCGGGCCTGACAATTTCCGCGCCAGCGTCAAGGCCGAGGTCAACACCGA
CACCCGCCAGACCGAAGAGACGATCTTCGATCCGAATTCGCGTGTCGAGCGCTCGGTGCAGTCGGTGCGCGCCAATGAGA
ACAGCAACCAGAAGCAGGCCTCGACCCCGGCCAGCGTCGAACAGAACCTGCCGGAGACCCAGGCGACCAGCACCGAGGGT
CCGCAAACCACCTCGGCGAACGACCGCAAGGAAGAGATCACCAATTACGAGATCAACTCCAAGAAGATCGCCACCGTCTC
CAACGGCTACACCGTCACCAAGATGTCGATCGCGGTCGTCGTCAACCAGGACAGGCTGAAGACGATCCTGGGCAAGGACG
CGACACCGGAGCAGATCGCCAAGCGCGTCGCCGAGATCCAGAAAATGGTGACGTCGGCCACCGGCCTCGACGACAAGCGT
GGCGACGTCATCGACGTCTCGGCGGTCGAGTTCATCGACGGGCTGGACGGCGAAGCGATCCCGCAGGCTGGAATGCTGGA
TTCCATCGGCCAGCACGCCGGCACGCTGATCAACGCCGGCGCCTTCATCGTCGTGGTGTTCCTGGTGGCCTTCTTCGGCC
TGCGGCCGATGGCGGCCGCGCTGACGGCAAGAGCAACGCCCGCTTTGTCGGGCCCGAACTTCGACGAAGTCCAGCGGTCG
CTGCCGACACCTGAGGCGGCTGCTTCCGCCGATGCGGGCGCCGCCATCGGCGCCTTGCCCGGCTCGCGGCCTGGCACCAA
TCCGCTCGATGATCTTCGCCAGAAGATCAGGCCGGCACCACAGGAGCGGCTTGCCCGCATGGTCGATCTCAACGAGGAGC
GCACAGCGCAGATCCTGCGCAAATGGGCGGCCCAGGAAGTCGCGGCGTAA

Upstream 100 bases:

>100_bases
CAATTCACGGGCCGCGCCGCAAGCGCGGCCCGATTTCATTTTCGCACAAGCTTCGAAGGCTAGTGTTCCGGCGGACAATC
ATGCTGGGAGTCGCTCTATC

Downstream 100 bases:

>100_bases
ACCATGGCCTCCGCAGCGCTTTTTGATCTTCTCCCTGATTTCGGTACGCGCGCGCCGCGCGTCGGCCAGCCCCAGGCCGC
GCCCGACCACAAGCCGGAAG

Product: flagellar MS-ring protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 549; Mature: 548

Protein sequence:

>549_residues
MPEQIQSIISNLRGFGVKRLAMLAGIAVLVMGVIGIASVYLNRPAYDTLYVGLDRADVNQIGLVLGEAGIGFDVGSDGTS
VLVPAGTTAQARMLLAEKGLPTSANAGYELFDNVGAMGLTSFMQQITRVRALEGEIARTIQSISGIKAARVHIVMSERAN
FRRDEQQPSASVVIRYAGIDAEKSAQSIRHLVAAAVPGLSADKVTVLDSNGNLLAAGDDPSNTSAARTLGVEQTVEAQIG
DNIRRALTPYLGPDNFRASVKAEVNTDTRQTEETIFDPNSRVERSVQSVRANENSNQKQASTPASVEQNLPETQATSTEG
PQTTSANDRKEEITNYEINSKKIATVSNGYTVTKMSIAVVVNQDRLKTILGKDATPEQIAKRVAEIQKMVTSATGLDDKR
GDVIDVSAVEFIDGLDGEAIPQAGMLDSIGQHAGTLINAGAFIVVVFLVAFFGLRPMAAALTARATPALSGPNFDEVQRS
LPTPEAAASADAGAAIGALPGSRPGTNPLDDLRQKIRPAPQERLARMVDLNEERTAQILRKWAAQEVAA

Sequences:

>Translated_549_residues
MPEQIQSIISNLRGFGVKRLAMLAGIAVLVMGVIGIASVYLNRPAYDTLYVGLDRADVNQIGLVLGEAGIGFDVGSDGTS
VLVPAGTTAQARMLLAEKGLPTSANAGYELFDNVGAMGLTSFMQQITRVRALEGEIARTIQSISGIKAARVHIVMSERAN
FRRDEQQPSASVVIRYAGIDAEKSAQSIRHLVAAAVPGLSADKVTVLDSNGNLLAAGDDPSNTSAARTLGVEQTVEAQIG
DNIRRALTPYLGPDNFRASVKAEVNTDTRQTEETIFDPNSRVERSVQSVRANENSNQKQASTPASVEQNLPETQATSTEG
PQTTSANDRKEEITNYEINSKKIATVSNGYTVTKMSIAVVVNQDRLKTILGKDATPEQIAKRVAEIQKMVTSATGLDDKR
GDVIDVSAVEFIDGLDGEAIPQAGMLDSIGQHAGTLINAGAFIVVVFLVAFFGLRPMAAALTARATPALSGPNFDEVQRS
LPTPEAAASADAGAAIGALPGSRPGTNPLDDLRQKIRPAPQERLARMVDLNEERTAQILRKWAAQEVAA
>Mature_548_residues
PEQIQSIISNLRGFGVKRLAMLAGIAVLVMGVIGIASVYLNRPAYDTLYVGLDRADVNQIGLVLGEAGIGFDVGSDGTSV
LVPAGTTAQARMLLAEKGLPTSANAGYELFDNVGAMGLTSFMQQITRVRALEGEIARTIQSISGIKAARVHIVMSERANF
RRDEQQPSASVVIRYAGIDAEKSAQSIRHLVAAAVPGLSADKVTVLDSNGNLLAAGDDPSNTSAARTLGVEQTVEAQIGD
NIRRALTPYLGPDNFRASVKAEVNTDTRQTEETIFDPNSRVERSVQSVRANENSNQKQASTPASVEQNLPETQATSTEGP
QTTSANDRKEEITNYEINSKKIATVSNGYTVTKMSIAVVVNQDRLKTILGKDATPEQIAKRVAEIQKMVTSATGLDDKRG
DVIDVSAVEFIDGLDGEAIPQAGMLDSIGQHAGTLINAGAFIVVVFLVAFFGLRPMAAALTARATPALSGPNFDEVQRSL
PTPEAAASADAGAAIGALPGSRPGTNPLDDLRQKIRPAPQERLARMVDLNEERTAQILRKWAAQEVAA

Specific function: The M ring may be actively involved in energy transduction [H]

COG id: COG1766

COG function: function code NU; Flagellar biosynthesis/type III secretory pathway lipoprotein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein. Bacterial flagellum basal body (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fliF family [H]

Homologues:

Organism=Escherichia coli, GI1788248, Length=414, Percent_Identity=29.951690821256, Blast_Score=155, Evalue=5e-39,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013556
- InterPro:   IPR000067
- InterPro:   IPR006182 [H]

Pfam domain/function: PF01514 YscJ_FliF; PF08345 YscJ_FliF_C [H]

EC number: NA

Molecular weight: Translated: 58211; Mature: 58080

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEQIQSIISNLRGFGVKRLAMLAGIAVLVMGVIGIASVYLNRPAYDTLYVGLDRADVNQ
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHH
IGLVLGEAGIGFDVGSDGTSVLVPAGTTAQARMLLAEKGLPTSANAGYELFDNVGAMGLT
HHHEEECCCCCCEECCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCHHHHHCCCHHHHH
SFMQQITRVRALEGEIARTIQSISGIKAARVHIVMSERANFRRDEQQPSASVVIRYAGID
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEECCC
AEKSAQSIRHLVAAAVPGLSADKVTVLDSNGNLLAAGDDPSNTSAARTLGVEQTVEAQIG
HHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEECCCCCCCCHHHHHHCHHHHHHHHHC
DNIRRALTPYLGPDNFRASVKAEVNTDTRQTEETIFDPNSRVERSVQSVRANENSNQKQA
HHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHC
STPASVEQNLPETQATSTEGPQTTSANDRKEEITNYEINSKKIATVSNGYTVTKMSIAVV
CCCCHHHHCCCCCCCCCCCCCCCCCCCCHHHHHCCEEECCCEEEEECCCEEEEEEEEEEE
VNQDRLKTILGKDATPEQIAKRVAEIQKMVTSATGLDDKRGDVIDVSAVEFIDGLDGEAI
ECHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHCCCCCCCC
PQAGMLDSIGQHAGTLINAGAFIVVVFLVAFFGLRPMAAALTARATPALSGPNFDEVQRS
CCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHH
LPTPEAAASADAGAAIGALPGSRPGTNPLDDLRQKIRPAPQERLARMVDLNEERTAQILR
CCCCHHHCCCCCCCEEECCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHH
KWAAQEVAA
HHHHHHHCC
>Mature Secondary Structure 
PEQIQSIISNLRGFGVKRLAMLAGIAVLVMGVIGIASVYLNRPAYDTLYVGLDRADVNQ
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHH
IGLVLGEAGIGFDVGSDGTSVLVPAGTTAQARMLLAEKGLPTSANAGYELFDNVGAMGLT
HHHEEECCCCCCEECCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCHHHHHCCCHHHHH
SFMQQITRVRALEGEIARTIQSISGIKAARVHIVMSERANFRRDEQQPSASVVIRYAGID
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEECCC
AEKSAQSIRHLVAAAVPGLSADKVTVLDSNGNLLAAGDDPSNTSAARTLGVEQTVEAQIG
HHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEECCCCCCCCHHHHHHCHHHHHHHHHC
DNIRRALTPYLGPDNFRASVKAEVNTDTRQTEETIFDPNSRVERSVQSVRANENSNQKQA
HHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHC
STPASVEQNLPETQATSTEGPQTTSANDRKEEITNYEINSKKIATVSNGYTVTKMSIAVV
CCCCHHHHCCCCCCCCCCCCCCCCCCCCHHHHHCCEEECCCEEEEECCCEEEEEEEEEEE
VNQDRLKTILGKDATPEQIAKRVAEIQKMVTSATGLDDKRGDVIDVSAVEFIDGLDGEAI
ECHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHCCCCCCCC
PQAGMLDSIGQHAGTLINAGAFIVVVFLVAFFGLRPMAAALTARATPALSGPNFDEVQRS
CCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHH
LPTPEAAASADAGAAIGALPGSRPGTNPLDDLRQKIRPAPQERLARMVDLNEERTAQILR
CCCCHHHCCCCCCCEEECCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHH
KWAAQEVAA
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9858749; 11481430 [H]