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The map label for this gene is nasD [H]

Identifier: 13472534

GI number: 13472534

Start: 2297518

End: 2299968

Strand: Direct

Name: nasD [H]

Synonym: mlr2862

Alternate gene names: 13472534

Gene position: 2297518-2299968 (Clockwise)

Preceding gene: 13472533

Following gene: 13472535

Centisome position: 32.65

GC content: 64.26

Gene sequence:

>2451_bases
ATGACAGAAAGATTAGTCATCATCGGCAACGGCATGGCCCCCGGGCGCATGCTGGAGCACCTGCTGGAACAGGCGCCGAG
CCGCTACAGCGTCACCATCTTCAACGCCGAACCGCGCGTCAATTACGACCGCATCATGCTGTCGCCGGTGCTGTCGGGCG
AAAAGGCCTATGAGGAAATCATCATCCATGGCGACGGCTGGTACATCGCCAACAACATCACCCTCTACAAGGGCCACAAG
ATCGTCGCCATCGACCGGGCGGCGAAGACCGTCACCTCCGATCATGGGGTCACCGAGCCCTACGACAAGCTGGTCATCGC
CACCGGCTCGGTGCCGTTCATCATTCCTGTACCCGGCCACAACCTGCCCGGCGTGCTGACCTATCGCGACCTCGACGACG
TACAGGCGATGATGCTGGCCGCCCAGTCGCGCGCCAAGGCCGTGGTGATCGGTGGCGGCCTGCTCGGGCTGGAAGCGGCG
GCGGGTCTCAACGCGCAAGGCATGGACGTCACCGTGCTGCATGTCATGCCGACACTGATGGAGCGCCAGCTCGATCCGGC
CGCCGGCTATCTCCTGCAGCGCGCGGTGGAGCAACGCGGCATCAAGGTCATCACCAAGGCCAACACGCAGGCAATCACGG
GCAATGGCAAGGTCGAGCAGGTGGAACTCGCCGACGGAACCGTTATCCCGGCGACCCTGGTGGTCATGGCGGTCGGCATC
AGGCCGAATTCGGCGCTGGCGAAAGAGGCAGGCATCGCCGTCAACAGAGGCATCGTCGTCGACGCGGGTATGCGCAGCAA
CGACCCCGACATCTACGCGCTAGGCGAATGCGCCGAGGTCAACGGCATGGTCTACGGGCTGGTGGCACCGCTCTACGAGA
TGGCACGCGTCGCGGCCCACCAGCTCGCCGGCAACGAGGCGGCCGCCTTCGTCCATATGGACACGCCGACCAAGCTCAAG
GTCACCGGCATCGACCTGTTCTCGCTCGGAGACTTCGCCGAGGGCGAGGACCGCCAGGAGATCGTGCTGCGCGACGCCGC
CGCCGGCGTCTACAAGCGGCTCGTTCTGAAGGACGACCGCATCATCGGCACCGTGCTCTATGGCGAGACGGCGGATGGTG
CCTGGTTCAACGACCTCAAGAAGAAGCAGACCGACATTTCGCAGATGCGCGATACGTTGATCTTCGGCCAGTCCTATCAG
GGGGGTGCCCCGCTGGACCCTATGGCGGCCGTTGCAGCCTTGCCGGATGATGCGGAAATCTGCGGCTGCAACGGCGTTTG
CAAGGGCAAGATCACAGGCGCGATCACGGCCAAGGGCCTGACTTCGCTCGACGATGTACGCGCCCACACCAAGGCGTCGG
CCTCCTGCGGCTCCTGCACCGGCCTGGTCGAGAAGCTGATGGTGCTGACCCTCGGCGACAGCTACAACCCTGCGGCAGTG
CAGCCGATGTGCACATGCACCACGCTCGGCCATGACGAGGTGCGCCGGCTGATCAAGGCCAAGCACCTGAAGACCATTCC
CGCCGTCATGCAGGAACTGGAATGGAAGACCTCCTGTGGCTGCGCCAAATGCCGGCCGGCGCTCAACTACTACCTCGTCT
GCGACTGGCCGGACGACTACGCCGACGACTACCAGTCACGCTTCATCAATGAGCGGGTGCATGCCAACATCCAGAAGGAC
GGCACCTATTCGGTGGTGCCGCGCATGTGGGGCGGCGTCACCAATGCCGCCGAACTGCGCGCCATCGCCGACGTCGTCGA
CAAGTTCGAGATCCCGATGGTCAAGGTCACCGGCGGCCAGCGCATCGACATGCTGGGCATCCGCAAGGAAGACCTGCCGG
CGGTGTGGGCAGATCTCGGCCAGGCGGGCTTCGTCTCCGGCCATGCCTATGCCAAGGGCCTGCGCACGGTGAAGACCTGC
GTCGGCTCCGACTGGTGCCGCTTCGGCACGCAGGATTCGACGGGTCTCGGCATCCGTATCGAGAAATTCATGTGGGGCTC
CTGGACGCCCGCCAAGGTCAAGATGGCGGTGTCGGGCTGCCCCAGAAACTGCGCCGAGGCGACCTGCAAGGATGTCGGCG
TCATCTGCGTCGACTCTGGCTACGAGATCCATTTCGCCGGCGCCGCCGGCCTCGACATCAAGGGCACCGAGGTGCTCGGC
CTGGTGAAGACCGAGGACGAGGCGCTGGAGCACATCGTGGCGCTGACGCAGATGTACCGTGAGCAGGGCCGCTATCTCGA
ACGCATCTACAAATGGGCCAAGCGCATCGGCATCCCCGAGATCAAGCGTCAGATCATGGACGACGATGCCAAGCGCAAGA
CCTATTACGAGCGCTTCGTCTTCTCCCAGAAATTCGCCCAGGTCGACCCGTGGTCGGAACGCGTCTCCGGCAAGGACAAG
CACGAGTTCCGGCCGATGGCTTCGGTCGGGTTTGCCGAGGCGGCGGAGTGA

Upstream 100 bases:

>100_bases
ATCGAACTCGCCTCCGACCGCACCTTCCTGCGCTGCCGCGAGGCCGTGCTGAAATTCCTCTACGAACGCCACCGCTTCGT
CGAAGCCGCGGAGTAGCATC

Downstream 100 bases:

>100_bases
TGGAGATGTCTGCGCGAGGCGCAATCCTATCAAGCAAAGGCAGCCCAATGAACTGGATCGCCATCGGCACCCTCTCCGAC
ATCCCGCGCCGTGGTGCGCG

Product: nitrite reductase large subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 816; Mature: 815

Protein sequence:

>816_residues
MTERLVIIGNGMAPGRMLEHLLEQAPSRYSVTIFNAEPRVNYDRIMLSPVLSGEKAYEEIIIHGDGWYIANNITLYKGHK
IVAIDRAAKTVTSDHGVTEPYDKLVIATGSVPFIIPVPGHNLPGVLTYRDLDDVQAMMLAAQSRAKAVVIGGGLLGLEAA
AGLNAQGMDVTVLHVMPTLMERQLDPAAGYLLQRAVEQRGIKVITKANTQAITGNGKVEQVELADGTVIPATLVVMAVGI
RPNSALAKEAGIAVNRGIVVDAGMRSNDPDIYALGECAEVNGMVYGLVAPLYEMARVAAHQLAGNEAAAFVHMDTPTKLK
VTGIDLFSLGDFAEGEDRQEIVLRDAAAGVYKRLVLKDDRIIGTVLYGETADGAWFNDLKKKQTDISQMRDTLIFGQSYQ
GGAPLDPMAAVAALPDDAEICGCNGVCKGKITGAITAKGLTSLDDVRAHTKASASCGSCTGLVEKLMVLTLGDSYNPAAV
QPMCTCTTLGHDEVRRLIKAKHLKTIPAVMQELEWKTSCGCAKCRPALNYYLVCDWPDDYADDYQSRFINERVHANIQKD
GTYSVVPRMWGGVTNAAELRAIADVVDKFEIPMVKVTGGQRIDMLGIRKEDLPAVWADLGQAGFVSGHAYAKGLRTVKTC
VGSDWCRFGTQDSTGLGIRIEKFMWGSWTPAKVKMAVSGCPRNCAEATCKDVGVICVDSGYEIHFAGAAGLDIKGTEVLG
LVKTEDEALEHIVALTQMYREQGRYLERIYKWAKRIGIPEIKRQIMDDDAKRKTYYERFVFSQKFAQVDPWSERVSGKDK
HEFRPMASVGFAEAAE

Sequences:

>Translated_816_residues
MTERLVIIGNGMAPGRMLEHLLEQAPSRYSVTIFNAEPRVNYDRIMLSPVLSGEKAYEEIIIHGDGWYIANNITLYKGHK
IVAIDRAAKTVTSDHGVTEPYDKLVIATGSVPFIIPVPGHNLPGVLTYRDLDDVQAMMLAAQSRAKAVVIGGGLLGLEAA
AGLNAQGMDVTVLHVMPTLMERQLDPAAGYLLQRAVEQRGIKVITKANTQAITGNGKVEQVELADGTVIPATLVVMAVGI
RPNSALAKEAGIAVNRGIVVDAGMRSNDPDIYALGECAEVNGMVYGLVAPLYEMARVAAHQLAGNEAAAFVHMDTPTKLK
VTGIDLFSLGDFAEGEDRQEIVLRDAAAGVYKRLVLKDDRIIGTVLYGETADGAWFNDLKKKQTDISQMRDTLIFGQSYQ
GGAPLDPMAAVAALPDDAEICGCNGVCKGKITGAITAKGLTSLDDVRAHTKASASCGSCTGLVEKLMVLTLGDSYNPAAV
QPMCTCTTLGHDEVRRLIKAKHLKTIPAVMQELEWKTSCGCAKCRPALNYYLVCDWPDDYADDYQSRFINERVHANIQKD
GTYSVVPRMWGGVTNAAELRAIADVVDKFEIPMVKVTGGQRIDMLGIRKEDLPAVWADLGQAGFVSGHAYAKGLRTVKTC
VGSDWCRFGTQDSTGLGIRIEKFMWGSWTPAKVKMAVSGCPRNCAEATCKDVGVICVDSGYEIHFAGAAGLDIKGTEVLG
LVKTEDEALEHIVALTQMYREQGRYLERIYKWAKRIGIPEIKRQIMDDDAKRKTYYERFVFSQKFAQVDPWSERVSGKDK
HEFRPMASVGFAEAAE
>Mature_815_residues
TERLVIIGNGMAPGRMLEHLLEQAPSRYSVTIFNAEPRVNYDRIMLSPVLSGEKAYEEIIIHGDGWYIANNITLYKGHKI
VAIDRAAKTVTSDHGVTEPYDKLVIATGSVPFIIPVPGHNLPGVLTYRDLDDVQAMMLAAQSRAKAVVIGGGLLGLEAAA
GLNAQGMDVTVLHVMPTLMERQLDPAAGYLLQRAVEQRGIKVITKANTQAITGNGKVEQVELADGTVIPATLVVMAVGIR
PNSALAKEAGIAVNRGIVVDAGMRSNDPDIYALGECAEVNGMVYGLVAPLYEMARVAAHQLAGNEAAAFVHMDTPTKLKV
TGIDLFSLGDFAEGEDRQEIVLRDAAAGVYKRLVLKDDRIIGTVLYGETADGAWFNDLKKKQTDISQMRDTLIFGQSYQG
GAPLDPMAAVAALPDDAEICGCNGVCKGKITGAITAKGLTSLDDVRAHTKASASCGSCTGLVEKLMVLTLGDSYNPAAVQ
PMCTCTTLGHDEVRRLIKAKHLKTIPAVMQELEWKTSCGCAKCRPALNYYLVCDWPDDYADDYQSRFINERVHANIQKDG
TYSVVPRMWGGVTNAAELRAIADVVDKFEIPMVKVTGGQRIDMLGIRKEDLPAVWADLGQAGFVSGHAYAKGLRTVKTCV
GSDWCRFGTQDSTGLGIRIEKFMWGSWTPAKVKMAVSGCPRNCAEATCKDVGVICVDSGYEIHFAGAAGLDIKGTEVLGL
VKTEDEALEHIVALTQMYREQGRYLERIYKWAKRIGIPEIKRQIMDDDAKRKTYYERFVFSQKFAQVDPWSERVSGKDKH
EFRPMASVGFAEAAE

Specific function: Required for nitrite assimilation [H]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI65787454, Length=315, Percent_Identity=26.6666666666667, Blast_Score=100, Evalue=6e-21,
Organism=Homo sapiens, GI21389617, Length=315, Percent_Identity=26.6666666666667, Blast_Score=100, Evalue=7e-21,
Organism=Homo sapiens, GI226437568, Length=315, Percent_Identity=26.6666666666667, Blast_Score=100, Evalue=9e-21,
Organism=Escherichia coli, GI1789765, Length=806, Percent_Identity=35.6079404466501, Blast_Score=444, Evalue=1e-125,
Organism=Escherichia coli, GI1789065, Length=322, Percent_Identity=30.1242236024845, Blast_Score=146, Evalue=6e-36,
Organism=Escherichia coli, GI1788892, Length=279, Percent_Identity=29.3906810035842, Blast_Score=110, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17559934, Length=410, Percent_Identity=24.6341463414634, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI281359715, Length=359, Percent_Identity=27.0194986072423, Blast_Score=121, Evalue=2e-27,
Organism=Drosophila melanogaster, GI281359713, Length=359, Percent_Identity=27.0194986072423, Blast_Score=121, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24639250, Length=359, Percent_Identity=27.0194986072423, Blast_Score=121, Evalue=2e-27,
Organism=Drosophila melanogaster, GI18543267, Length=359, Percent_Identity=27.0194986072423, Blast_Score=121, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24639252, Length=359, Percent_Identity=27.0194986072423, Blast_Score=121, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24639257, Length=358, Percent_Identity=27.0949720670391, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24585130, Length=326, Percent_Identity=23.6196319018405, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24581020, Length=240, Percent_Identity=25.4166666666667, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI28573993, Length=240, Percent_Identity=25.4166666666667, Blast_Score=69, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 88693; Mature: 88562

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTERLVIIGNGMAPGRMLEHLLEQAPSRYSVTIFNAEPRVNYDRIMLSPVLSGEKAYEEI
CCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEEHHHCCCCCEEEE
IIHGDGWYIANNITLYKGHKIVAIDRAAKTVTSDHGVTEPYDKLVIATGSVPFIIPVPGH
EEECCCEEEECEEEEEECCEEEEEECHHHHHCCCCCCCCCCCCEEEEECCCCEEEECCCC
NLPGVLTYRDLDDVQAMMLAAQSRAKAVVIGGGLLGLEAAAGLNAQGMDVTVLHVMPTLM
CCCCEEEECCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHCCCCCCCCCEEHHHHHHHHH
ERQLDPAAGYLLQRAVEQRGIKVITKANTQAITGNGKVEQVELADGTVIPATLVVMAVGI
HHCCCHHHHHHHHHHHHHCCEEEEEECCCEEEECCCCEEEEEECCCCCHHHHHHHHEECC
RPNSALAKEAGIAVNRGIVVDAGMRSNDPDIYALGECAEVNGMVYGLVAPLYEMARVAAH
CCCHHHHHHCCCEEECCEEEECCCCCCCCCEEEECCCHHHCCCHHHHHHHHHHHHHHHHH
QLAGNEAAAFVHMDTPTKLKVTGIDLFSLGDFAEGEDRQEIVLRDAAAGVYKRLVLKDDR
HHCCCCEEEEEEECCCCEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
IIGTVLYGETADGAWFNDLKKKQTDISQMRDTLIFGQSYQGGAPLDPMAAVAALPDDAEI
EEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHHCCCCCCCE
CGCNGVCKGKITGAITAKGLTSLDDVRAHTKASASCGSCTGLVEKLMVLTLGDSYNPAAV
ECCCCEECCCEEEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHEEECCCCCCCCC
QPMCTCTTLGHDEVRRLIKAKHLKTIPAVMQELEWKTSCGCAKCRPALNYYLVCDWPDDY
CCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCEEEEECCCCHH
ADDYQSRFINERVHANIQKDGTYSVVPRMWGGVTNAAELRAIADVVDKFEIPMVKVTGGQ
HHHHHHHHHHHHHHCCCCCCCCEEECHHHHCCCCCHHHHHHHHHHHHHHCCCEEEECCCC
RIDMLGIRKEDLPAVWADLGQAGFVSGHAYAKGLRTVKTCVGSDWCRFGTQDSTGLGIRI
EEEEECCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCEEE
EKFMWGSWTPAKVKMAVSGCPRNCAEATCKDVGVICVDSGYEIHFAGAAGLDIKGTEVLG
EEEECCCCCCCEEEEEECCCCCHHHHHHHHCCCEEEECCCCEEEEECCCCCCCCCCEEEE
LVKTEDEALEHIVALTQMYREQGRYLERIYKWAKRIGIPEIKRQIMDDDAKRKTYYERFV
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHH
FSQKFAQVDPWSERVSGKDKHEFRPMASVGFAEAAE
HHHHHHCCCCHHHHCCCCCCHHCCCHHHCCCHHCCC
>Mature Secondary Structure 
TERLVIIGNGMAPGRMLEHLLEQAPSRYSVTIFNAEPRVNYDRIMLSPVLSGEKAYEEI
CCEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEEHHHCCCCCEEEE
IIHGDGWYIANNITLYKGHKIVAIDRAAKTVTSDHGVTEPYDKLVIATGSVPFIIPVPGH
EEECCCEEEECEEEEEECCEEEEEECHHHHHCCCCCCCCCCCCEEEEECCCCEEEECCCC
NLPGVLTYRDLDDVQAMMLAAQSRAKAVVIGGGLLGLEAAAGLNAQGMDVTVLHVMPTLM
CCCCEEEECCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHCCCCCCCCCEEHHHHHHHHH
ERQLDPAAGYLLQRAVEQRGIKVITKANTQAITGNGKVEQVELADGTVIPATLVVMAVGI
HHCCCHHHHHHHHHHHHHCCEEEEEECCCEEEECCCCEEEEEECCCCCHHHHHHHHEECC
RPNSALAKEAGIAVNRGIVVDAGMRSNDPDIYALGECAEVNGMVYGLVAPLYEMARVAAH
CCCHHHHHHCCCEEECCEEEECCCCCCCCCEEEECCCHHHCCCHHHHHHHHHHHHHHHHH
QLAGNEAAAFVHMDTPTKLKVTGIDLFSLGDFAEGEDRQEIVLRDAAAGVYKRLVLKDDR
HHCCCCEEEEEEECCCCEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
IIGTVLYGETADGAWFNDLKKKQTDISQMRDTLIFGQSYQGGAPLDPMAAVAALPDDAEI
EEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHHCCCCCCCE
CGCNGVCKGKITGAITAKGLTSLDDVRAHTKASASCGSCTGLVEKLMVLTLGDSYNPAAV
ECCCCEECCCEEEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHEEECCCCCCCCC
QPMCTCTTLGHDEVRRLIKAKHLKTIPAVMQELEWKTSCGCAKCRPALNYYLVCDWPDDY
CCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCEEEEECCCCHH
ADDYQSRFINERVHANIQKDGTYSVVPRMWGGVTNAAELRAIADVVDKFEIPMVKVTGGQ
HHHHHHHHHHHHHHCCCCCCCCEEECHHHHCCCCCHHHHHHHHHHHHHHCCCEEEECCCC
RIDMLGIRKEDLPAVWADLGQAGFVSGHAYAKGLRTVKTCVGSDWCRFGTQDSTGLGIRI
EEEEECCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCEEE
EKFMWGSWTPAKVKMAVSGCPRNCAEATCKDVGVICVDSGYEIHFAGAAGLDIKGTEVLG
EEEECCCCCCCEEEEEECCCCCHHHHHHHHCCCEEEECCCCEEEEECCCCCCCCCCEEEE
LVKTEDEALEHIVALTQMYREQGRYLERIYKWAKRIGIPEIKRQIMDDDAKRKTYYERFV
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHH
FSQKFAQVDPWSERVSGKDKHEFRPMASVGFAEAAE
HHHHHHCCCCHHHHCCCCCCHHCCCHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]