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The map label for this gene is cls [H]

Identifier: 13472525

GI number: 13472525

Start: 2289310

End: 2290773

Strand: Direct

Name: cls [H]

Synonym: mlr2852

Alternate gene names: 13472525

Gene position: 2289310-2290773 (Clockwise)

Preceding gene: 13472515

Following gene: 13472526

Centisome position: 32.54

GC content: 64.96

Gene sequence:

>1464_bases
ATGTTCGAGACCATTGCGACTCACTGGACACAAATCCTGGCGATCATCTCGGTGGTCATGGCGACCGTCGGCATCGCCCA
TGCCGTCATGACCAAGGAGGATGTGCGCGCCGCCACCGGCTGGGTCGGCGTGATGGTGCTGTCGCCGATCCTTGGCGTGC
TGATCTATGCCGTGGCCGGCATCAACCGCATCCGCCGCGCCACGATCACGGCGCAGCGCCCGCTTGCCGGCGACGCGGTA
TCCGCCAAGAATGAGCGCGACATCGCCGCCGAGGAGACCCTGATCGTCGAGCGCTACGGTCAGCGCTTCACCGGCCTGCG
GACGCTGGGCGACAGGGTGGCGCGGCGCGCCCTCAACCCGGGCAATGCCATCGATGTGCTGGAGACCGGCGACGAGGCCT
ATGCCGCCATGTGCGCGGCGATAGACGGCGCCGAGCGCAGCGTCCTGCTCGAGACCTATATTTTCGACAATGACGCTGTC
GGCCTGCTTTTCGTCGAATCGCTGGCCGGTGCCGTCCAGCGTGGTGTCACCGTTCGCGTGCTGATCGACGCCGTCGGTGC
CCGTTACTCCGTTCCCAGCATCCTGGGCCATCTGCGCCGCGCCAACATCCCCGCCGATGTCTTCAACGGCAACATCATCA
TAGGCCTGCGCCTGCCCTACGCCAATCTCCGGACCCACCGGAAGATCCTTGTGGTCGACGGCACCGTTGCCTTCACCGGC
GGCATGAACATCCGCAAGGGTTTTTCGGCCGAGTTCTCAGGCTCCAACAGCGCCAGGGACACGCATTTCAAGGTCACCGG
ACCTGCCGTCGCCGACCTGTTCTCGGTCGCGGCCGAAGACTGGCGCTTCGCCACCAACGAAGCGCTGAAGGGCGATGCCT
GGCGAATTGCCCCGCTGTCGCCGGCGCCCGGCCAGCCGATGCTGGTGCGGGCGGTCGCATCCGGGCCGGACGCCAGCAAT
GAGACCAACCACAAGCTGTTGATCGGCGCCTTCTCGGTGGCGCGCAAGTCGATCCGTTTGATGTCGCCCTATTTCCTGCC
GGACCGCGAACTGATCAGCGCCTTGATCACCGCCGCCCGGCGCGGCGTCGAGATCGATGTCGTGGTGCCGGCGGTCAACA
ATCTCTTCCTCGTCGACCGCGCCATGACCGCGCAATTCGACCAGATCCTGAAGAATTATTGCCGCATATGGCGCACGGAA
GGCCCGTTCGACCATTCCAAGCTGCTGTCGATCGACGGCGTGTGGGCCTATGTCGGCTCGTCCAACCTCGACGCCCGGTC
GCTGCGGTTGAACTTCGAGATCGATCTGGAGGTCCTCGATGCAGGCTTTGCCCGCGAGATCGAGGCCCGGATCGGATCGG
CCATCGAAACGGCCATTCCCGTCACGCTGGACTCCTTGCGGGCCCGGCCCTTCATCATCCGGCTCTTCGACCGCATCCTG
TGGCTGGGATCGCCCTATCTTTAG

Upstream 100 bases:

>100_bases
GACTAGATCTCTCCAGCCATGTCGCGCCCGCCACCTGGCGAATGTGCGGCATTGACCGGCCGCATTGCCTTCTGCAACCG
CTCGCTGGATAGTCCCGGCA

Downstream 100 bases:

>100_bases
AAGGCTTTAACAAAACGACGTGTTTGACGGATAGTTTGGTGTCGGCCATGGAACATAAGCGCAAAGACTCTATTTGAGGT
GCATGGTGAGACCAGCAGGC

Product: cardiolipin synthase

Products: NA

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MFETIATHWTQILAIISVVMATVGIAHAVMTKEDVRAATGWVGVMVLSPILGVLIYAVAGINRIRRATITAQRPLAGDAV
SAKNERDIAAEETLIVERYGQRFTGLRTLGDRVARRALNPGNAIDVLETGDEAYAAMCAAIDGAERSVLLETYIFDNDAV
GLLFVESLAGAVQRGVTVRVLIDAVGARYSVPSILGHLRRANIPADVFNGNIIIGLRLPYANLRTHRKILVVDGTVAFTG
GMNIRKGFSAEFSGSNSARDTHFKVTGPAVADLFSVAAEDWRFATNEALKGDAWRIAPLSPAPGQPMLVRAVASGPDASN
ETNHKLLIGAFSVARKSIRLMSPYFLPDRELISALITAARRGVEIDVVVPAVNNLFLVDRAMTAQFDQILKNYCRIWRTE
GPFDHSKLLSIDGVWAYVGSSNLDARSLRLNFEIDLEVLDAGFAREIEARIGSAIETAIPVTLDSLRARPFIIRLFDRIL
WLGSPYL

Sequences:

>Translated_487_residues
MFETIATHWTQILAIISVVMATVGIAHAVMTKEDVRAATGWVGVMVLSPILGVLIYAVAGINRIRRATITAQRPLAGDAV
SAKNERDIAAEETLIVERYGQRFTGLRTLGDRVARRALNPGNAIDVLETGDEAYAAMCAAIDGAERSVLLETYIFDNDAV
GLLFVESLAGAVQRGVTVRVLIDAVGARYSVPSILGHLRRANIPADVFNGNIIIGLRLPYANLRTHRKILVVDGTVAFTG
GMNIRKGFSAEFSGSNSARDTHFKVTGPAVADLFSVAAEDWRFATNEALKGDAWRIAPLSPAPGQPMLVRAVASGPDASN
ETNHKLLIGAFSVARKSIRLMSPYFLPDRELISALITAARRGVEIDVVVPAVNNLFLVDRAMTAQFDQILKNYCRIWRTE
GPFDHSKLLSIDGVWAYVGSSNLDARSLRLNFEIDLEVLDAGFAREIEARIGSAIETAIPVTLDSLRARPFIIRLFDRIL
WLGSPYL
>Mature_487_residues
MFETIATHWTQILAIISVVMATVGIAHAVMTKEDVRAATGWVGVMVLSPILGVLIYAVAGINRIRRATITAQRPLAGDAV
SAKNERDIAAEETLIVERYGQRFTGLRTLGDRVARRALNPGNAIDVLETGDEAYAAMCAAIDGAERSVLLETYIFDNDAV
GLLFVESLAGAVQRGVTVRVLIDAVGARYSVPSILGHLRRANIPADVFNGNIIIGLRLPYANLRTHRKILVVDGTVAFTG
GMNIRKGFSAEFSGSNSARDTHFKVTGPAVADLFSVAAEDWRFATNEALKGDAWRIAPLSPAPGQPMLVRAVASGPDASN
ETNHKLLIGAFSVARKSIRLMSPYFLPDRELISALITAARRGVEIDVVVPAVNNLFLVDRAMTAQFDQILKNYCRIWRTE
GPFDHSKLLSIDGVWAYVGSSNLDARSLRLNFEIDLEVLDAGFAREIEARIGSAIETAIPVTLDSLRARPFIIRLFDRIL
WLGSPYL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=485, Percent_Identity=27.4226804123711, Blast_Score=172, Evalue=5e-44,
Organism=Escherichia coli, GI1787007, Length=344, Percent_Identity=28.1976744186047, Blast_Score=124, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 52992; Mature: 52992

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFETIATHWTQILAIISVVMATVGIAHAVMTKEDVRAATGWVGVMVLSPILGVLIYAVAG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
INRIRRATITAQRPLAGDAVSAKNERDIAAEETLIVERYGQRFTGLRTLGDRVARRALNP
HHHHHHHHEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCC
GNAIDVLETGDEAYAAMCAAIDGAERSVLLETYIFDNDAVGLLFVESLAGAVQRGVTVRV
CCEEEEEECCCHHHHHHHHHHCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHCCEEEE
LIDAVGARYSVPSILGHLRRANIPADVFNGNIIIGLRLPYANLRTHRKILVVDGTVAFTG
EEEHHCCCCCHHHHHHHHHHCCCCHHHCCCCEEEEEECCCCCCCCCCEEEEEECEEEEEC
GMNIRKGFSAEFSGSNSARDTHFKVTGPAVADLFSVAAEDWRFATNEALKGDAWRIAPLS
CCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECC
PAPGQPMLVRAVASGPDASNETNHKLLIGAFSVARKSIRLMSPYFLPDRELISALITAAR
CCCCCCEEEEEEECCCCCCCCCCCEEEEEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
RGVEIDVVVPAVNNLFLVDRAMTAQFDQILKNYCRIWRTEGPFDHSKLLSIDGVWAYVGS
CCCEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEECC
SNLDARSLRLNFEIDLEVLDAGFAREIEARIGSAIETAIPVTLDSLRARPFIIRLFDRIL
CCCCCEEEEEEEEEEEEEECCCHHHHHHHHHCCHHHHHHCHHHHHHCCCHHHHHHHHHHH
WLGSPYL
HCCCCCC
>Mature Secondary Structure
MFETIATHWTQILAIISVVMATVGIAHAVMTKEDVRAATGWVGVMVLSPILGVLIYAVAG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
INRIRRATITAQRPLAGDAVSAKNERDIAAEETLIVERYGQRFTGLRTLGDRVARRALNP
HHHHHHHHEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCC
GNAIDVLETGDEAYAAMCAAIDGAERSVLLETYIFDNDAVGLLFVESLAGAVQRGVTVRV
CCEEEEEECCCHHHHHHHHHHCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHCCEEEE
LIDAVGARYSVPSILGHLRRANIPADVFNGNIIIGLRLPYANLRTHRKILVVDGTVAFTG
EEEHHCCCCCHHHHHHHHHHCCCCHHHCCCCEEEEEECCCCCCCCCCEEEEEECEEEEEC
GMNIRKGFSAEFSGSNSARDTHFKVTGPAVADLFSVAAEDWRFATNEALKGDAWRIAPLS
CCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECC
PAPGQPMLVRAVASGPDASNETNHKLLIGAFSVARKSIRLMSPYFLPDRELISALITAAR
CCCCCCEEEEEEECCCCCCCCCCCEEEEEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
RGVEIDVVVPAVNNLFLVDRAMTAQFDQILKNYCRIWRTEGPFDHSKLLSIDGVWAYVGS
CCCEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEECC
SNLDARSLRLNFEIDLEVLDAGFAREIEARIGSAIETAIPVTLDSLRARPFIIRLFDRIL
CCCCCEEEEEEEEEEEEEECCCHHHHHHHHHCCHHHHHHCHHHHHHCCCHHHHHHHHHHH
WLGSPYL
HCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9443601 [H]