BacMap

Definition	Mesorhizobium loti MAFF303099 chromosome, complete genome.
Accession	NC_002678
Length	7,036,071

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The map label for this gene is fabG2 [H]

Identifier: 13472489

GI number: 13472489

Start: 2249885

End: 2250646

Strand: Direct

Name: fabG2 [H]

Synonym: mlr2803

Alternate gene names: 13472489

Gene position: 2249885-2250646 (Clockwise)

Preceding gene: 13472488

Following gene: 13472490

Centisome position: 31.98

GC content: 66.67

Gene sequence:

>762_bases
ATGAACCCGAACGGCCAGATCGCGATCGTCACCGGCGGCGGCTCCGGCCTTGGCGAGGCAACGGCGCGCGCGCTTGCCGC
CAAGGGTGCCCGCGTCGCCATCTTCGATGTCGGTATCGAGCGCGCGGCCAAGGTCGCGGCCGACATCGGCGGCATTTCCG
TCCAATGCGACGTCAGCAGCGCCGACAGTGGCACTGCGGCCCTCGCCGAGACAGCGAGCAAGCTCGGCGAGCCACGCATC
CTGGTCAATTGCGCCGGCATCGCCATTGGCGTGAAGACCATCGGCAAGGACGGACCGCATCCGCTCGATCAATACCGCAA
GGTTATCGAGGTCAATCTGATCGGCACCTTCAACATGATCCGCCTCGTCGCCGACCGCGCCGCCAGCCTCGAGCCGCTGC
AGGGCGGCGAGCGTGGTGTCATCGTCAACACCGCTTCGGTCGCCGCCTATGACGGCCAGATCGGCCAGGCCGCCTACTCC
GCTTCGAAGGGCGGCGTGGTCGGCATGACCCTGCCGGTGGCGCGCGACCTTGCCCGTTCCGGCATCCGCGTCTGCACCAT
CGCACCCGGCATCTTCAAGACACCGATGATGGCCGGCATGCCGCAGGAGGTGCAGGATTCGCTCGGCGCCGCCGTGCCCT
TCCCGTCCCGTCTCGGCGAACCATCCGAATATGCGGCACTTGCCTTGCACATCATCGAAAACCAGATGCTGAACGGCGAA
ACCATCCGTCTCGACGGCGCCATCCGCATGGCGCCGAAATAG

Upstream 100 bases:

>100_bases
GGGCCTGATCCGTCCCTGCTTTCTTGGAGCAATTCCAGGAAGATGTGAAACGGTTCGGCGCTTTCGTGAATGATGAATTG
CTCCGGGAGAGGATGACGCC

Downstream 100 bases:

>100_bases
TATAAGTCACCCGGAAGTGCGATGCGGCTCCAGGCGACTTGCATAGAACGCTTGCCAGGACCGGCATCATCGTCTGATAA
GTGGAACAAGAAAATCAACT

Product: 3-hydroxyacyl-CoA dehydrogenase type II

Products: 3-oxoacyl-CoA; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MNPNGQIAIVTGGGSGLGEATARALAAKGARVAIFDVGIERAAKVAADIGGISVQCDVSSADSGTAALAETASKLGEPRI
LVNCAGIAIGVKTIGKDGPHPLDQYRKVIEVNLIGTFNMIRLVADRAASLEPLQGGERGVIVNTASVAAYDGQIGQAAYS
ASKGGVVGMTLPVARDLARSGIRVCTIAPGIFKTPMMAGMPQEVQDSLGAAVPFPSRLGEPSEYAALALHIIENQMLNGE
TIRLDGAIRMAPK

Sequences:

>Translated_253_residues
MNPNGQIAIVTGGGSGLGEATARALAAKGARVAIFDVGIERAAKVAADIGGISVQCDVSSADSGTAALAETASKLGEPRI
LVNCAGIAIGVKTIGKDGPHPLDQYRKVIEVNLIGTFNMIRLVADRAASLEPLQGGERGVIVNTASVAAYDGQIGQAAYS
ASKGGVVGMTLPVARDLARSGIRVCTIAPGIFKTPMMAGMPQEVQDSLGAAVPFPSRLGEPSEYAALALHIIENQMLNGE
TIRLDGAIRMAPK
>Mature_253_residues
MNPNGQIAIVTGGGSGLGEATARALAAKGARVAIFDVGIERAAKVAADIGGISVQCDVSSADSGTAALAETASKLGEPRI
LVNCAGIAIGVKTIGKDGPHPLDQYRKVIEVNLIGTFNMIRLVADRAASLEPLQGGERGVIVNTASVAAYDGQIGQAAYS
ASKGGVVGMTLPVARDLARSGIRVCTIAPGIFKTPMMAGMPQEVQDSLGAAVPFPSRLGEPSEYAALALHIIENQMLNGE
TIRLDGAIRMAPK

Specific function: Fatty acid biosynthesis pathway; first reduction step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI4758504, Length=254, Percent_Identity=54.3307086614173, Blast_Score=258, Evalue=4e-69,
Organism=Homo sapiens, GI83715985, Length=254, Percent_Identity=51.1811023622047, Blast_Score=236, Evalue=2e-62,
Organism=Homo sapiens, GI15277342, Length=259, Percent_Identity=32.4324324324324, Blast_Score=115, Evalue=3e-26,
Organism=Homo sapiens, GI40254992, Length=250, Percent_Identity=31.2, Blast_Score=111, Evalue=8e-25,
Organism=Homo sapiens, GI31542939, Length=262, Percent_Identity=27.0992366412214, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI224922801, Length=177, Percent_Identity=30.5084745762712, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI59889578, Length=232, Percent_Identity=27.1551724137931, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI4504505, Length=217, Percent_Identity=29.4930875576037, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI19923817, Length=263, Percent_Identity=25.4752851711027, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI22507405, Length=228, Percent_Identity=28.0701754385965, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI40807363, Length=207, Percent_Identity=28.9855072463768, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI5031737, Length=198, Percent_Identity=30.8080808080808, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI33667109, Length=196, Percent_Identity=31.1224489795918, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI32483357, Length=250, Percent_Identity=28, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1787335, Length=258, Percent_Identity=30.6201550387597, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI87082100, Length=259, Percent_Identity=30.5019305019305, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1787905, Length=239, Percent_Identity=33.0543933054393, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788459, Length=259, Percent_Identity=28.1853281853282, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1789057, Length=212, Percent_Identity=31.1320754716981, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1789208, Length=252, Percent_Identity=28.1746031746032, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1790717, Length=262, Percent_Identity=27.4809160305344, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI2367175, Length=252, Percent_Identity=32.1428571428571, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI87082160, Length=254, Percent_Identity=31.1023622047244, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1789378, Length=235, Percent_Identity=27.6595744680851, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786812, Length=201, Percent_Identity=30.8457711442786, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17538182, Length=249, Percent_Identity=51.4056224899598, Blast_Score=245, Evalue=1e-65,
Organism=Caenorhabditis elegans, GI25147288, Length=257, Percent_Identity=35.7976653696498, Blast_Score=130, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17555706, Length=253, Percent_Identity=35.1778656126482, Blast_Score=127, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17567345, Length=202, Percent_Identity=32.1782178217822, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17561402, Length=265, Percent_Identity=32.0754716981132, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17538480, Length=200, Percent_Identity=32, Blast_Score=77, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17560332, Length=222, Percent_Identity=30.6306306306306, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17562904, Length=213, Percent_Identity=30.9859154929577, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17560676, Length=261, Percent_Identity=26.0536398467433, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17563726, Length=215, Percent_Identity=27.4418604651163, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17568967, Length=217, Percent_Identity=29.0322580645161, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17559104, Length=214, Percent_Identity=30.3738317757009, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17560150, Length=264, Percent_Identity=26.1363636363636, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17551412, Length=229, Percent_Identity=29.2576419213974, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17536651, Length=258, Percent_Identity=27.5193798449612, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17561272, Length=257, Percent_Identity=31.9066147859922, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI72000259, Length=263, Percent_Identity=26.2357414448669, Blast_Score=65, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17562910, Length=263, Percent_Identity=26.9961977186312, Blast_Score=64, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6322861, Length=196, Percent_Identity=31.1224489795918, Blast_Score=82, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6320089, Length=202, Percent_Identity=27.7227722772277, Blast_Score=62, Evalue=7e-11,
Organism=Drosophila melanogaster, GI17737361, Length=250, Percent_Identity=54.8, Blast_Score=268, Evalue=3e-72,
Organism=Drosophila melanogaster, GI24639444, Length=254, Percent_Identity=36.2204724409449, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI21355319, Length=252, Percent_Identity=30.1587301587302, Blast_Score=84, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24644339, Length=236, Percent_Identity=31.3559322033898, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21357041, Length=231, Percent_Identity=29.004329004329, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI221331218, Length=217, Percent_Identity=30.4147465437788, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24665243, Length=217, Percent_Identity=30.4147465437788, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI18859837, Length=202, Percent_Identity=33.1683168316832, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI221331216, Length=218, Percent_Identity=30.2752293577982, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24665247, Length=218, Percent_Identity=30.2752293577982, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI23397609, Length=201, Percent_Identity=29.3532338308458, Blast_Score=64, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.35

Molecular weight: Translated: 25815; Mature: 25815

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPNGQIAIVTGGGSGLGEATARALAAKGARVAIFDVGIERAAKVAADIGGISVQCDVSS
CCCCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHCCCEEEEEECCC
ADSGTAALAETASKLGEPRILVNCAGIAIGVKTIGKDGPHPLDQYRKVIEVNLIGTFNMI
CCCCHHHHHHHHHHHCCCEEEEEECCEEEEEEHHCCCCCCCHHHHHHEEEEEEECHHHHH
RLVADRAASLEPLQGGERGVIVNTASVAAYDGQIGQAAYSASKGGVVGMTLPVARDLARS
HHHHHHHCCCCCCCCCCCEEEEEECEEEEECCCCCHHHHCCCCCCEEEEEHHHHHHHHHC
GIRVCTIAPGIFKTPMMAGMPQEVQDSLGAAVPFPSRLGEPSEYAALALHIIENQMLNGE
CCEEEEECCCHHHCCHHCCCCHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHHHHCCCCC
TIRLDGAIRMAPK
EEEECCEEEECCC
>Mature Secondary Structure
MNPNGQIAIVTGGGSGLGEATARALAAKGARVAIFDVGIERAAKVAADIGGISVQCDVSS
CCCCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHCCCEEEEEECCC
ADSGTAALAETASKLGEPRILVNCAGIAIGVKTIGKDGPHPLDQYRKVIEVNLIGTFNMI
CCCCHHHHHHHHHHHCCCEEEEEECCEEEEEEHHCCCCCCCHHHHHHEEEEEEECHHHHH
RLVADRAASLEPLQGGERGVIVNTASVAAYDGQIGQAAYSASKGGVVGMTLPVARDLARS
HHHHHHHCCCCCCCCCCCEEEEEECEEEEECCCCCHHHHCCCCCCEEEEEHHHHHHHHHC
GIRVCTIAPGIFKTPMMAGMPQEVQDSLGAAVPFPSRLGEPSEYAALALHIIENQMLNGE
CCEEEEECCCHHHCCHHCCCCHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHHHHCCCCC
TIRLDGAIRMAPK
EEEECCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-3-hydroxyacyl-CoA; NAD+

Specific reaction: (S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]