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The map label for this gene is trpE(G) [H]

Identifier: 13472468

GI number: 13472468

Start: 2227590

End: 2229791

Strand: Direct

Name: trpE(G) [H]

Synonym: mlr2774

Alternate gene names: 13472468

Gene position: 2227590-2229791 (Clockwise)

Preceding gene: 13472466

Following gene: 13472469

Centisome position: 31.66

GC content: 63.71

Gene sequence:

>2202_bases
ATGGAGACGGCAATGACGATGAAGGTTCTGGAAAACGGCGCTGAAAGCTTCGTGACCGCGGGTGGCATCACCATTACGCG
TGAGCGCCACGACCGGCCCTATGCGGGTGCGATCGACGCTTATGTCGATGGCTTGAACTCGCGCCGCGGCGCGGTGTTTT
CCTCCAACTACGAATATCCCGGCCGCTATACGCGCTGGGACACCGCCATCATCGATCCGCCGCTGGTCATTTCCGCGCGG
GGCCGCGCCATGCGCATCGAAGCGCTGAACCGCCGCGGCGAGGCGCTGTTGCCGGTGATCGGCAAAACGCTGGGCGGCCT
TGCCGACATCACCATCGCCGAGACGACGAAGACGCTCATCCGCCTCGACGTCGCCAAGCCCGGCCGCGTCTTCACGGAGG
AAGAGCGCAGCCGCGTGCCCTCGGTCTTCACCGTGCTGCGCGCCATCACCGCTTTGTTCAAAACCGACGAGGACGCCAAT
CTCGGCCTCTATGGCGCCTTCGGCTACGACCTCTCCTTCCAGTTCGACCCGGTCGACTACAAGCTCGAGCGCAAGCCCAG
CCAGCGCGACCTCGTGCTGTTCCTGCCCGACGAGATCCTGGTCGTCGACCACTATTCCGCCAAAGCCTGGACCGACCGCT
ACGACTATTCGGGCGAAGGATTTTCGACCGAAGGTCTGCCGCGCGACGCAATCGCCGAGCCGTTCAAGACCGCCGACCGC
ATCCCGCCGCGCGGTGACCATGAGCCGGGCGAATACGCTAATCTGGTGCGGCGTGCCATGGACTCGTTCAAGCGCGGCGA
CCTGTTCGAGGTCGTGCCCGGCCAGATGTTCTACGAGCGCTGCGAGACGCAGCCCTCCGACATTTCGCGCAAGCTGAAAT
CGATCAACCCCTCGCCTTATTCGTTCTTCATCAACCTCGGCGAAAACGAATATCTGATCGGCGCCTCGCCCGAAATGTTC
GTGCGCGTCAATGGCCGCCGCGTCGAAACCTGCCCGATTTCGGGCACCATCAAACGCGGCGACGACGCCATTTCCGATAG
CGAGCAGATCCTGAAGCTGCTCAATTCGAAGAAGGACGAATCCGAGCTCACAATGTGCTCGGACGTCGACCGCAACGACA
AGTCGCGGGTCTGCGAGCCCGGCTCGGTGCGCGTCATCGGCCGCCGCCAGATCGAGATGTATTCGCGCCTCATCCACACC
GTCGATCACATCGAAGGCCGGCTGCGCGAAGGCATGGACGCTTTCGACGCCTTCCTGTCGCATGCCTGGGCGGTCACTGT
CACCGGCGCGCCGAAACTGTGGGCCATGCGCTTCATCGAGCAGAACGAGAAGAGCCCGCGCGCCTGGTATGGCGGCGCAA
TCGGCATGGTCAACTTCAACGGCGACATGAACACCGGCCTGACGCTGCGCACCATCCGCATCAAGGACGGCATTGCCGAA
GTGCGGGCCGGCGCGACATTGCTGTTCGACAGCATTCCCGAGGAAGAAGAAGCCGAAACCGAACTGAAGGCATCCGCCAT
GCTCTCCGCCATCCGCGACGCCAAGACGGGCAACTCCGCCAGCACCGAGCGCACCACCGCGCGGGTCGGCGACGGCGTCA
ACATCCTGCTCGTCGACCACGAGGACTCTTTCGTCCACACGCTGGCCAACTACTTCCGCCAGACCGGCGCCAATGTCTCG
ACCGTGCGCACGCCGGTGCCGGACGAAGTGTTCGAGCGGCTGAAGCCGGACCTTGTCGTGCTCTCACCCGGACCGGGTAC
GCCGAAGGATTTCGATTGCGCCGCGACCATCAGACGAGCGCGCGCCCGCGACCTGCCGATCTTCGGCGTCTGCCTAGGCC
TGCAGGCGCTGGCCGAGGCCTATGGCGGGGAACTGCGCCAGCTGCATATTCCCATGCACGGCAAGCCCTCGCGCATCCGC
GTCTCCAAGCCCGGCATCATCTTCTCCGGCCTGCCCAAGGAAGTCACTGTCGGCCGTTACCACTCGATCTTCGCCGATCC
GGTGCGCTTGCCCGATGATTTCATTGTCACGGCAGAGACTGAGGACGGCATCATCATGGCTTTCGAGCACCGCAAGGAGC
CGATCGCGGCGGTGCAGTTCCACCCGGAATCGATCATGACGCTCGGCCACAATGCCGGCATGCGCATCATCGAGAACATC
GTCGCCCATTTGCCGCGCAAGGCCAAGGAAAAGGCAGCCTGA

Upstream 100 bases:

>100_bases
CGAACGCGCGTGCGTGAAAAGAGAGGGTGGCCGCAACGGAAAGTCCGGGCGGCCATTTGTTTTTTAAAAACCAGTCCCGG
GTCCGTTGCCCAACAAGCTG

Downstream 100 bases:

>100_bases
CCTCGATGGCCGAAGCCGAGAACACGGTGGCGATCGCAGCCAGGGCGGCCGCGAAGCGCAAGGTCGCCAACCTTGCCTTC
TGGTCGATCGCGGTCGCCTT

Product: anthranilate synthase

Products: NA

Alternate protein names: Glutamine amidotransferase [H]

Number of amino acids: Translated: 733; Mature: 733

Protein sequence:

>733_residues
METAMTMKVLENGAESFVTAGGITITRERHDRPYAGAIDAYVDGLNSRRGAVFSSNYEYPGRYTRWDTAIIDPPLVISAR
GRAMRIEALNRRGEALLPVIGKTLGGLADITIAETTKTLIRLDVAKPGRVFTEEERSRVPSVFTVLRAITALFKTDEDAN
LGLYGAFGYDLSFQFDPVDYKLERKPSQRDLVLFLPDEILVVDHYSAKAWTDRYDYSGEGFSTEGLPRDAIAEPFKTADR
IPPRGDHEPGEYANLVRRAMDSFKRGDLFEVVPGQMFYERCETQPSDISRKLKSINPSPYSFFINLGENEYLIGASPEMF
VRVNGRRVETCPISGTIKRGDDAISDSEQILKLLNSKKDESELTMCSDVDRNDKSRVCEPGSVRVIGRRQIEMYSRLIHT
VDHIEGRLREGMDAFDAFLSHAWAVTVTGAPKLWAMRFIEQNEKSPRAWYGGAIGMVNFNGDMNTGLTLRTIRIKDGIAE
VRAGATLLFDSIPEEEEAETELKASAMLSAIRDAKTGNSASTERTTARVGDGVNILLVDHEDSFVHTLANYFRQTGANVS
TVRTPVPDEVFERLKPDLVVLSPGPGTPKDFDCAATIRRARARDLPIFGVCLGLQALAEAYGGELRQLHIPMHGKPSRIR
VSKPGIIFSGLPKEVTVGRYHSIFADPVRLPDDFIVTAETEDGIIMAFEHRKEPIAAVQFHPESIMTLGHNAGMRIIENI
VAHLPRKAKEKAA

Sequences:

>Translated_733_residues
METAMTMKVLENGAESFVTAGGITITRERHDRPYAGAIDAYVDGLNSRRGAVFSSNYEYPGRYTRWDTAIIDPPLVISAR
GRAMRIEALNRRGEALLPVIGKTLGGLADITIAETTKTLIRLDVAKPGRVFTEEERSRVPSVFTVLRAITALFKTDEDAN
LGLYGAFGYDLSFQFDPVDYKLERKPSQRDLVLFLPDEILVVDHYSAKAWTDRYDYSGEGFSTEGLPRDAIAEPFKTADR
IPPRGDHEPGEYANLVRRAMDSFKRGDLFEVVPGQMFYERCETQPSDISRKLKSINPSPYSFFINLGENEYLIGASPEMF
VRVNGRRVETCPISGTIKRGDDAISDSEQILKLLNSKKDESELTMCSDVDRNDKSRVCEPGSVRVIGRRQIEMYSRLIHT
VDHIEGRLREGMDAFDAFLSHAWAVTVTGAPKLWAMRFIEQNEKSPRAWYGGAIGMVNFNGDMNTGLTLRTIRIKDGIAE
VRAGATLLFDSIPEEEEAETELKASAMLSAIRDAKTGNSASTERTTARVGDGVNILLVDHEDSFVHTLANYFRQTGANVS
TVRTPVPDEVFERLKPDLVVLSPGPGTPKDFDCAATIRRARARDLPIFGVCLGLQALAEAYGGELRQLHIPMHGKPSRIR
VSKPGIIFSGLPKEVTVGRYHSIFADPVRLPDDFIVTAETEDGIIMAFEHRKEPIAAVQFHPESIMTLGHNAGMRIIENI
VAHLPRKAKEKAA
>Mature_733_residues
METAMTMKVLENGAESFVTAGGITITRERHDRPYAGAIDAYVDGLNSRRGAVFSSNYEYPGRYTRWDTAIIDPPLVISAR
GRAMRIEALNRRGEALLPVIGKTLGGLADITIAETTKTLIRLDVAKPGRVFTEEERSRVPSVFTVLRAITALFKTDEDAN
LGLYGAFGYDLSFQFDPVDYKLERKPSQRDLVLFLPDEILVVDHYSAKAWTDRYDYSGEGFSTEGLPRDAIAEPFKTADR
IPPRGDHEPGEYANLVRRAMDSFKRGDLFEVVPGQMFYERCETQPSDISRKLKSINPSPYSFFINLGENEYLIGASPEMF
VRVNGRRVETCPISGTIKRGDDAISDSEQILKLLNSKKDESELTMCSDVDRNDKSRVCEPGSVRVIGRRQIEMYSRLIHT
VDHIEGRLREGMDAFDAFLSHAWAVTVTGAPKLWAMRFIEQNEKSPRAWYGGAIGMVNFNGDMNTGLTLRTIRIKDGIAE
VRAGATLLFDSIPEEEEAETELKASAMLSAIRDAKTGNSASTERTTARVGDGVNILLVDHEDSFVHTLANYFRQTGANVS
TVRTPVPDEVFERLKPDLVVLSPGPGTPKDFDCAATIRRARARDLPIFGVCLGLQALAEAYGGELRQLHIPMHGKPSRIR
VSKPGIIFSGLPKEVTVGRYHSIFADPVRLPDDFIVTAETEDGIIMAFEHRKEPIAAVQFHPESIMTLGHNAGMRIIENI
VAHLPRKAKEKAA

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=472, Percent_Identity=30.0847457627119, Blast_Score=182, Evalue=5e-47,
Organism=Escherichia coli, GI1788114, Length=364, Percent_Identity=29.3956043956044, Blast_Score=140, Evalue=3e-34,
Organism=Escherichia coli, GI1789760, Length=188, Percent_Identity=41.4893617021277, Blast_Score=132, Evalue=9e-32,
Organism=Escherichia coli, GI1787517, Length=198, Percent_Identity=36.8686868686869, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI1786809, Length=224, Percent_Identity=29.0178571428571, Blast_Score=86, Evalue=9e-18,
Organism=Escherichia coli, GI1786215, Length=151, Percent_Identity=27.8145695364238, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320935, Length=271, Percent_Identity=38.3763837638376, Blast_Score=166, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6322638, Length=192, Percent_Identity=39.0625, Blast_Score=128, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6324361, Length=209, Percent_Identity=27.2727272727273, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR006805
- InterPro:   IPR006220
- InterPro:   IPR010112
- InterPro:   IPR001317
- InterPro:   IPR015890
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR006221 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind; PF00117 GATase [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 81465; Mature: 81465

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METAMTMKVLENGAESFVTAGGITITRERHDRPYAGAIDAYVDGLNSRRGAVFSSNYEYP
CCCHHHHHHHHCCHHHHEEECCEEEEECCCCCCCCCHHHHHHHCCCCCCCCEEECCCCCC
GRYTRWDTAIIDPPLVISARGRAMRIEALNRRGEALLPVIGKTLGGLADITIAETTKTLI
CCCCCCCCEECCCCEEEECCCCEEEEHHHCCCCCCHHHHHHHHHCCCEEEEEHHHCEEEE
RLDVAKPGRVFTEEERSRVPSVFTVLRAITALFKTDEDANLGLYGAFGYDLSFQFDPVDY
EEECCCCCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEECCCCE
KLERKPSQRDLVLFLPDEILVVDHYSAKAWTDRYDYSGEGFSTEGLPRDAIAEPFKTADR
EECCCCCCCCEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCHHHHCC
IPPRGDHEPGEYANLVRRAMDSFKRGDLFEVVPGQMFYERCETQPSDISRKLKSINPSPY
CCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHCCCHHHHHHHHHHCCCCCE
SFFINLGENEYLIGASPEMFVRVNGRRVETCPISGTIKRGDDAISDSEQILKLLNSKKDE
EEEEEECCCCEEEECCCCEEEEECCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCC
SELTMCSDVDRNDKSRVCEPGSVRVIGRRQIEMYSRLIHTVDHIEGRLREGMDAFDAFLS
CHHHEHHCCCCCCHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
HAWAVTVTGAPKLWAMRFIEQNEKSPRAWYGGAIGMVNFNGDMNTGLTLRTIRIKDGIAE
CCEEEEECCCCHHHHHHHHHHCCCCCCEEECCEEEEEEECCCCCCCEEEEEEEECCCHHH
VRAGATLLFDSIPEEEEAETELKASAMLSAIRDAKTGNSASTERTTARVGDGVNILLVDH
HHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCEEEEEEEC
EDSFVHTLANYFRQTGANVSTVRTPVPDEVFERLKPDLVVLSPGPGTPKDFDCAATIRRA
CCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHH
RARDLPIFGVCLGLQALAEAYGGELRQLHIPMHGKPSRIRVSKPGIIFSGLPKEVTVGRY
HHCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEECCCEEEECCCCCCCCCHH
HSIFADPVRLPDDFIVTAETEDGIIMAFEHRKEPIAAVQFHPESIMTLGHNAGMRIIENI
HHHHCCCCCCCCCEEEEEECCCCEEEEEECCCCCEEEEEECCHHHHHCCCCCCHHHHHHH
VAHLPRKAKEKAA
HHHCCHHHHHCCC
>Mature Secondary Structure
METAMTMKVLENGAESFVTAGGITITRERHDRPYAGAIDAYVDGLNSRRGAVFSSNYEYP
CCCHHHHHHHHCCHHHHEEECCEEEEECCCCCCCCCHHHHHHHCCCCCCCCEEECCCCCC
GRYTRWDTAIIDPPLVISARGRAMRIEALNRRGEALLPVIGKTLGGLADITIAETTKTLI
CCCCCCCCEECCCCEEEECCCCEEEEHHHCCCCCCHHHHHHHHHCCCEEEEEHHHCEEEE
RLDVAKPGRVFTEEERSRVPSVFTVLRAITALFKTDEDANLGLYGAFGYDLSFQFDPVDY
EEECCCCCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEECCCCE
KLERKPSQRDLVLFLPDEILVVDHYSAKAWTDRYDYSGEGFSTEGLPRDAIAEPFKTADR
EECCCCCCCCEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCHHHHCC
IPPRGDHEPGEYANLVRRAMDSFKRGDLFEVVPGQMFYERCETQPSDISRKLKSINPSPY
CCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHCCCHHHHHHHHHHCCCCCE
SFFINLGENEYLIGASPEMFVRVNGRRVETCPISGTIKRGDDAISDSEQILKLLNSKKDE
EEEEEECCCCEEEECCCCEEEEECCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCC
SELTMCSDVDRNDKSRVCEPGSVRVIGRRQIEMYSRLIHTVDHIEGRLREGMDAFDAFLS
CHHHEHHCCCCCCHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
HAWAVTVTGAPKLWAMRFIEQNEKSPRAWYGGAIGMVNFNGDMNTGLTLRTIRIKDGIAE
CCEEEEECCCCHHHHHHHHHHCCCCCCEEECCEEEEEEECCCCCCCEEEEEEEECCCHHH
VRAGATLLFDSIPEEEEAETELKASAMLSAIRDAKTGNSASTERTTARVGDGVNILLVDH
HHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCEEEEEEEC
EDSFVHTLANYFRQTGANVSTVRTPVPDEVFERLKPDLVVLSPGPGTPKDFDCAATIRRA
CCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHH
RARDLPIFGVCLGLQALAEAYGGELRQLHIPMHGKPSRIRVSKPGIIFSGLPKEVTVGRY
HHCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEECCCEEEECCCCCCCCCHH
HSIFADPVRLPDDFIVTAETEDGIIMAFEHRKEPIAAVQFHPESIMTLGHNAGMRIIENI
HHHHCCCCCCCCCEEEEEECCCCEEEEEECCCCCEEEEEECCHHHHHCCCCCCHHHHHHH
VAHLPRKAKEKAA
HHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656657; 11481430 [H]