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The map label for this gene is greA

Identifier: 13472314

GI number: 13472314

Start: 2078428

End: 2078901

Strand: Reverse

Name: greA

Synonym: mll2568

Alternate gene names: 13472314

Gene position: 2078901-2078428 (Counterclockwise)

Preceding gene: 13472315

Following gene: 13472310

Centisome position: 29.55

GC content: 62.66

Gene sequence:

>474_bases
ATGAACAAGGTCCCGATGACAGGCGAGGGGTTCGCGTCATTGAAGGAAGAACTGCGTTGGCGCCAGCAGGAAGAGCGGCC
GCGCATCATCGAGGCGATCTCCGAGGCGCGCTCGCATGGCGACCTGTCCGAAAATGCCGAATACCACGCTGCCAAGGAGG
CGCAGAGCCTCAATGAGGGCCGTGTCAACGAGCTCGAGGACCTGATCGCGCGCGCCGAGGTGATCGACGTCACCAAGCTC
AGCGGCGACAAGGTCAAGTTCGGCGCCACCGTCGTGCTGGTCGATGAGGACACCGAGGAAAAGAAGACCTACCAGATCGT
CGGCGACCAGGAAGCCGACGTGAAATCCGGCCGCATCTCGATCTCCTCGCCGATCGCGCGCGCATTGATCGGCAAGGAAG
TCGGCGACGCCATCGAGGTCAATGCGCCCGGCGGTGCCAGGGGTTACGAGATCGTCCAGGTGCAGTTCATCTAG

Upstream 100 bases:

>100_bases
CGGTTCCGTTAGCCCGAGGCTGCCGGACCGTTTTCTTTTTGTACCCAACGACAAGGCTTCCCCCGGAACGCGGGGGTGGC
GGCAGGAAAGGTCAGGCATC

Downstream 100 bases:

>100_bases
GACCTTTTAATAATTCTACTCTGGCGCGGTTTCTGCGCTTCCGGTGTTCACGTACTTTAAAGTACGCTCCGCTCGTCGCG
GGGCGACCCGAAAACCACGC

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MNKVPMTGEGFASLKEELRWRQQEERPRIIEAISEARSHGDLSENAEYHAAKEAQSLNEGRVNELEDLIARAEVIDVTKL
SGDKVKFGATVVLVDEDTEEKKTYQIVGDQEADVKSGRISISSPIARALIGKEVGDAIEVNAPGGARGYEIVQVQFI

Sequences:

>Translated_157_residues
MNKVPMTGEGFASLKEELRWRQQEERPRIIEAISEARSHGDLSENAEYHAAKEAQSLNEGRVNELEDLIARAEVIDVTKL
SGDKVKFGATVVLVDEDTEEKKTYQIVGDQEADVKSGRISISSPIARALIGKEVGDAIEVNAPGGARGYEIVQVQFI
>Mature_157_residues
MNKVPMTGEGFASLKEELRWRQQEERPRIIEAISEARSHGDLSENAEYHAAKEAQSLNEGRVNELEDLIARAEVIDVTKL
SGDKVKFGATVVLVDEDTEEKKTYQIVGDQEADVKSGRISISSPIARALIGKEVGDAIEVNAPGGARGYEIVQVQFI

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family

Homologues:

Organism=Escherichia coli, GI87082225, Length=158, Percent_Identity=50, Blast_Score=162, Evalue=1e-41,
Organism=Escherichia coli, GI87082261, Length=156, Percent_Identity=35.8974358974359, Blast_Score=92, Evalue=1e-20,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): GREA_RHILO (Q98I49)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_103881.1
- ProteinModelPortal:   Q98I49
- SMR:   Q98I49
- GeneID:   1226542
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll2568
- NMPDR:   fig|266835.1.peg.1985
- HOGENOM:   HBG753387
- OMA:   TPGGFVK
- ProtClustDB:   PRK00226
- GO:   GO:0006350
- HAMAP:   MF_00105
- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691
- Gene3D:   G3DSA:1.10.287.180
- Gene3D:   G3DSA:3.10.50.30
- TIGRFAMs:   TIGR01462

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N; SSF46557 GreA_GreB

EC number: NA

Molecular weight: Translated: 17251; Mature: 17251

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS00829 GREAB_1; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKVPMTGEGFASLKEELRWRQQEERPRIIEAISEARSHGDLSENAEYHAAKEAQSLNEG
CCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCC
RVNELEDLIARAEVIDVTKLSGDKVKFGATVVLVDEDTEEKKTYQIVGDQEADVKSGRIS
CHHHHHHHHHHHHHHHEEECCCCEEEECEEEEEEECCCCCCEEEEEECCCCCCCCCCEEE
ISSPIARALIGKEVGDAIEVNAPGGARGYEIVQVQFI
ECHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEEEEC
>Mature Secondary Structure
MNKVPMTGEGFASLKEELRWRQQEERPRIIEAISEARSHGDLSENAEYHAAKEAQSLNEG
CCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCC
RVNELEDLIARAEVIDVTKLSGDKVKFGATVVLVDEDTEEKKTYQIVGDQEADVKSGRIS
CHHHHHHHHHHHHHHHEEECCCCEEEECEEEEEEECCCCCCEEEEEECCCCCCCCCCEEE
ISSPIARALIGKEVGDAIEVNAPGGARGYEIVQVQFI
ECHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968