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The map label for this gene is dnaG [H]

Identifier: 13472239

GI number: 13472239

Start: 2002532

End: 2004490

Strand: Reverse

Name: dnaG [H]

Synonym: mll2467

Alternate gene names: 13472239

Gene position: 2004490-2002532 (Counterclockwise)

Preceding gene: 13472240

Following gene: 13472238

Centisome position: 28.49

GC content: 64.98

Gene sequence:

>1959_bases
ATGGTGGGGAAACGAGTTCCCTCAATGCGCTTTCCACCCGCCTTTCTCGACGAGATCCGCGACCGCGTGCCGATTTCACA
GGTCATCGGCCAGCGCGTCGCGTGGGATCGCAGGAAGACCAATGCGTCGCGTGGCGATTTTTGGGCCTGCTGCCCGTTCC
ATGGCGAGAAGAGCCCGTCCTTCCATTGCGAGGACAAGAAGGGCCGCTACCATTGTTTCGGCTGCTCGGTCTCGGGCGAT
CATTTCAAATTCCTGACCGAACTCGACGGAATGAGTTTCCCTGAAGCGGTCGAGAAGATCGCCGACATGGCCGGCGTGCC
GATGCCGGTGCGCGACGCCCAGGAGGAACGGCGCGAGAAGGAACGCGCGAGCCTGACCGACGTCATGGAGATGGCGACCG
CCTTTTTCCAGGAACGGCTGCAGGGGCCTGAGGGCGCAAAGGCCCGCGCCTATCTGCGCGACCGTGGATTGACGCCGGCA
ACGCAGCAGTCGTTCCGGCTGGGCTACGCGCCGGACAGCCGCAATGCGCTCAAGGAGCATCTCGCGGCAAAAGGCGTGCC
GAAGGCCGATATCGAAGCCTGCGGGCTGGTACGACACGGCGACGACATTCCGGTCTCCTATGACTGGTTCCGCGACCGCA
TCATGTTCCCGATTCCGGATTCGCGCGGCAAGATCATCGCTTTTGGGGGCCGGGCGCTGGCGCCCGATGCGCTGGCCAAA
TACATGAACTCGCCCGACACCGAGCTCTTCCACAAGGGCAATGTGCTCTACAATTTCGCCCGCGCCCGCAAGGCACTCGC
CAAGGACGGCACGGTCATTGCCGTCGAAGGTTATATGGACGTGATCGCGCTGGCGCAGGCCGGCTTCGAGAATGTTGTGG
CGCCGCTCGGCACGGCGCTCACCGAAAACCAGCTGGAATTGCTTTGGCGTATGGCGCCCGAACCGATGCTGTGTTTCGAC
GGCGACAAGGCCGGGCTGAAAGCGGCATGGCGTGCAGCCGATCTGGCACTGCCATCGGTGCAGGCCGGGCGCTCGGCGCG
CTTCGCGCTGCTGCCGGAAGGCAAGGATCCCGACGACCTCGTCAAGGCCGAGGGGCCGGACGCTTTCCGCGCGGTGCTTG
CCGATGCGCGGCCGCTGGTCGACCTGTTGTGGATGCGCGAGACGGCGGGCGGTGTCTTCGACACGCCTGAGCGACGGGCG
GAACTGGGAAAGACGCTGCGGGAACTCGCCAGCCGCATCCGCGACGAAAGCACGCGCTATCACTACCAGCAGGAAATGCG
TGAGCGCGAAAAGAGCTTCTTCGGCTCGCAGCGCGATGCGCGGCAAGGGCGCCAGGACTGGAAACCAGGACAGGGCAAGG
CAGCCGCACCCGGTGGCCAATTCGCCAAGCCTGGCGGCGGCCGCATGGCGATCACCGAAAGCCTTGGCCAGTCGGCGTTG
GTCAAGCGCGGCAGCGAGGGCATGTCGGTGCGCGAGGCGACGATCCTCGTGGCGCTGGTCAACCATCCGCCGCTGATCGA
CGAGAATTTCGCCCATGTCGAATTTCTCGACCTCGCCAATTCCGAATTGCAGCGGCTGCACGCCGCCATCCTCGACGCGA
TGGCGCATGACATGGCCAATGACCGCCACGCCGTGGTGGCGACGATCGAGCGTGCCGGCTGCGCCGAGATCTGGGAGCGC
GCCGTCGGCCTGATCCGGCGGGCGCGGCAATGGCCCGCGCTGGAGACGGCGGCACTCGACGACGCCCGCGACGCCCTGAA
CCAGGCGCTGCACTTGCAGCGCAGCGCGCGCACCTTACATAAGGAACTGAAACAGGCGGAAGCGGCGCTCGAAGCAGATC
CCTCGGACGAAAACTTCCGCCATCTGATCGAAATTCAGGCGCAATTTCAGGATGTACAGGCGACGGAAGCGCTGATCGAA
GGATTCGGCGTTTCGTCGGGCAGGGCTGGGCGAGCCTAA

Upstream 100 bases:

>100_bases
CCTGGAAAGGAGGGCGGTCCGTATGGCCGCCGTCTTTTTGCCCTGTGGAAACCGGGTATGGCCAGCATTGGGGGTCGTCG
GATTTAGCTGGCATGATTAT

Downstream 100 bases:

>100_bases
GTGCAGCTGAGTACAGGAATGAGAGCGTGGCCAGGCTTCCCAGGGAAGCAAGGCGCGCTAAGTCGGGTAATTGATTCGCT
TTTTTCATGCGAATCATGCC

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 652; Mature: 652

Protein sequence:

>652_residues
MVGKRVPSMRFPPAFLDEIRDRVPISQVIGQRVAWDRRKTNASRGDFWACCPFHGEKSPSFHCEDKKGRYHCFGCSVSGD
HFKFLTELDGMSFPEAVEKIADMAGVPMPVRDAQEERREKERASLTDVMEMATAFFQERLQGPEGAKARAYLRDRGLTPA
TQQSFRLGYAPDSRNALKEHLAAKGVPKADIEACGLVRHGDDIPVSYDWFRDRIMFPIPDSRGKIIAFGGRALAPDALAK
YMNSPDTELFHKGNVLYNFARARKALAKDGTVIAVEGYMDVIALAQAGFENVVAPLGTALTENQLELLWRMAPEPMLCFD
GDKAGLKAAWRAADLALPSVQAGRSARFALLPEGKDPDDLVKAEGPDAFRAVLADARPLVDLLWMRETAGGVFDTPERRA
ELGKTLRELASRIRDESTRYHYQQEMREREKSFFGSQRDARQGRQDWKPGQGKAAAPGGQFAKPGGGRMAITESLGQSAL
VKRGSEGMSVREATILVALVNHPPLIDENFAHVEFLDLANSELQRLHAAILDAMAHDMANDRHAVVATIERAGCAEIWER
AVGLIRRARQWPALETAALDDARDALNQALHLQRSARTLHKELKQAEAALEADPSDENFRHLIEIQAQFQDVQATEALIE
GFGVSSGRAGRA

Sequences:

>Translated_652_residues
MVGKRVPSMRFPPAFLDEIRDRVPISQVIGQRVAWDRRKTNASRGDFWACCPFHGEKSPSFHCEDKKGRYHCFGCSVSGD
HFKFLTELDGMSFPEAVEKIADMAGVPMPVRDAQEERREKERASLTDVMEMATAFFQERLQGPEGAKARAYLRDRGLTPA
TQQSFRLGYAPDSRNALKEHLAAKGVPKADIEACGLVRHGDDIPVSYDWFRDRIMFPIPDSRGKIIAFGGRALAPDALAK
YMNSPDTELFHKGNVLYNFARARKALAKDGTVIAVEGYMDVIALAQAGFENVVAPLGTALTENQLELLWRMAPEPMLCFD
GDKAGLKAAWRAADLALPSVQAGRSARFALLPEGKDPDDLVKAEGPDAFRAVLADARPLVDLLWMRETAGGVFDTPERRA
ELGKTLRELASRIRDESTRYHYQQEMREREKSFFGSQRDARQGRQDWKPGQGKAAAPGGQFAKPGGGRMAITESLGQSAL
VKRGSEGMSVREATILVALVNHPPLIDENFAHVEFLDLANSELQRLHAAILDAMAHDMANDRHAVVATIERAGCAEIWER
AVGLIRRARQWPALETAALDDARDALNQALHLQRSARTLHKELKQAEAALEADPSDENFRHLIEIQAQFQDVQATEALIE
GFGVSSGRAGRA
>Mature_652_residues
MVGKRVPSMRFPPAFLDEIRDRVPISQVIGQRVAWDRRKTNASRGDFWACCPFHGEKSPSFHCEDKKGRYHCFGCSVSGD
HFKFLTELDGMSFPEAVEKIADMAGVPMPVRDAQEERREKERASLTDVMEMATAFFQERLQGPEGAKARAYLRDRGLTPA
TQQSFRLGYAPDSRNALKEHLAAKGVPKADIEACGLVRHGDDIPVSYDWFRDRIMFPIPDSRGKIIAFGGRALAPDALAK
YMNSPDTELFHKGNVLYNFARARKALAKDGTVIAVEGYMDVIALAQAGFENVVAPLGTALTENQLELLWRMAPEPMLCFD
GDKAGLKAAWRAADLALPSVQAGRSARFALLPEGKDPDDLVKAEGPDAFRAVLADARPLVDLLWMRETAGGVFDTPERRA
ELGKTLRELASRIRDESTRYHYQQEMREREKSFFGSQRDARQGRQDWKPGQGKAAAPGGQFAKPGGGRMAITESLGQSAL
VKRGSEGMSVREATILVALVNHPPLIDENFAHVEFLDLANSELQRLHAAILDAMAHDMANDRHAVVATIERAGCAEIWER
AVGLIRRARQWPALETAALDDARDALNQALHLQRSARTLHKELKQAEAALEADPSDENFRHLIEIQAQFQDVQATEALIE
GFGVSSGRAGRA

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=422, Percent_Identity=40.2843601895735, Blast_Score=304, Evalue=1e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 72147; Mature: 72147

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGKRVPSMRFPPAFLDEIRDRVPISQVIGQRVAWDRRKTNASRGDFWACCPFHGEKSPS
CCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCC
FHCEDKKGRYHCFGCSVSGDHFKFLTELDGMSFPEAVEKIADMAGVPMPVRDAQEERREK
CCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHH
ERASLTDVMEMATAFFQERLQGPEGAKARAYLRDRGLTPATQQSFRLGYAPDSRNALKEH
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHCEECCCCCCHHHHHHH
LAAKGVPKADIEACGLVRHGDDIPVSYDWFRDRIMFPIPDSRGKIIAFGGRALAPDALAK
HHHCCCCCCCHHHHCCEECCCCCCCCHHHHHCCEEECCCCCCCCEEEECCCCCCHHHHHH
YMNSPDTELFHKGNVLYNFARARKALAKDGTVIAVEGYMDVIALAQAGFENVVAPLGTAL
HHCCCCHHHHHCCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCHHHHHHHHHHHH
TENQLELLWRMAPEPMLCFDGDKAGLKAAWRAADLALPSVQAGRSARFALLPEGKDPDDL
HHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCHHHH
VKAEGPDAFRAVLADARPLVDLLWMRETAGGVFDTPERRAELGKTLRELASRIRDESTRY
HCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
HYQQEMREREKSFFGSQRDARQGRQDWKPGQGKAAAPGGQFAKPGGGRMAITESLGQSAL
HHHHHHHHHHHHHCCCCHHHHCCHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCHHHH
VKRGSEGMSVREATILVALVNHPPLIDENFAHVEFLDLANSELQRLHAAILDAMAHDMAN
HHCCCCCCCEEHHEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
DRHAVVATIERAGCAEIWERAVGLIRRARQWPALETAALDDARDALNQALHLQRSARTLH
CCEEEEEEHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KELKQAEAALEADPSDENFRHLIEIQAQFQDVQATEALIEGFGVSSGRAGRA
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MVGKRVPSMRFPPAFLDEIRDRVPISQVIGQRVAWDRRKTNASRGDFWACCPFHGEKSPS
CCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCC
FHCEDKKGRYHCFGCSVSGDHFKFLTELDGMSFPEAVEKIADMAGVPMPVRDAQEERREK
CCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHH
ERASLTDVMEMATAFFQERLQGPEGAKARAYLRDRGLTPATQQSFRLGYAPDSRNALKEH
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHCEECCCCCCHHHHHHH
LAAKGVPKADIEACGLVRHGDDIPVSYDWFRDRIMFPIPDSRGKIIAFGGRALAPDALAK
HHHCCCCCCCHHHHCCEECCCCCCCCHHHHHCCEEECCCCCCCCEEEECCCCCCHHHHHH
YMNSPDTELFHKGNVLYNFARARKALAKDGTVIAVEGYMDVIALAQAGFENVVAPLGTAL
HHCCCCHHHHHCCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCHHHHHHHHHHHH
TENQLELLWRMAPEPMLCFDGDKAGLKAAWRAADLALPSVQAGRSARFALLPEGKDPDDL
HHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCHHHH
VKAEGPDAFRAVLADARPLVDLLWMRETAGGVFDTPERRAELGKTLRELASRIRDESTRY
HCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
HYQQEMREREKSFFGSQRDARQGRQDWKPGQGKAAAPGGQFAKPGGGRMAITESLGQSAL
HHHHHHHHHHHHHCCCCHHHHCCHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCHHHH
VKRGSEGMSVREATILVALVNHPPLIDENFAHVEFLDLANSELQRLHAAILDAMAHDMAN
HHCCCCCCCEEHHEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
DRHAVVATIERAGCAEIWERAVGLIRRARQWPALETAALDDARDALNQALHLQRSARTLH
CCEEEEEEHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KELKQAEAALEADPSDENFRHLIEIQAQFQDVQATEALIEGFGVSSGRAGRA
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]