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The map label for this gene is azoR

Identifier: 13472205

GI number: 13472205

Start: 1971786

End: 1972418

Strand: Direct

Name: azoR

Synonym: mlr2421

Alternate gene names: 13472205

Gene position: 1971786-1972418 (Clockwise)

Preceding gene: 13472203

Following gene: 13472206

Centisome position: 28.02

GC content: 65.88

Gene sequence:

>633_bases
ATGTCCATTCTTCTCGTTACCTCGAGCCCGCGCGGCGCTGCCTCGCACTCGACCCGCATCGCCACCGAATTCGCCGAAAA
GCTCCTCGCCGCCGATCCGTCGAACACGCTTGTCGTGCGCGATCTCGTCGCCAACCCGCTGCCGCATATCGATGCCGACT
ACGCGACCGGCATCTACACGCCGGTTGAAGCGCGCACCCCGCGCCAGGCTGAAGTCGTCGGCGTCTCCGATGTCGTGCTC
GACGAGCTGTTCGCCGCCGACACGGTGATCCTCGCCACCGGCTTCATCAACTTCAACATCTCCTCGACGCTGAAGTCCTG
GGTCGACCACATCGCCCGCTCCGGCCGCAGCTTTGCCTATGGCGAGAATGGCCCCAAGGGCCTCGTCACCGGCAAGAAGG
TCTATATCGTGCTGGCCTCGGGCGGCATCTACTCGGAAGGTGCGGCCGTGCAGTTCGATCACGCCATTCCCTATCTGCGC
GGCGTGCTCGGCTTCCTGGGTATGACCGATGTCGACGTCATCCGCATCGAAGGCGTCGGCATGGGCCCTGACGCGGTCAC
CGCCGCGCTCGCCAAGGCGACCGCCAAGGTCGACGCCGTGGTCGCCAGCCAGCAGGCCGCTGCTGCCGCGTAA

Upstream 100 bases:

>100_bases
TCTATTTTGTGGATGGATCGTATATAAAATATACCAGTTTATCTGATGGATGGATATCCCATATCTAGCGGGACAATTCA
TTCCACTGGAGAAACCCGCT

Downstream 100 bases:

>100_bases
GGCGCTTCGATCATCTGAACTTGAAAGGCAGGCGCCCGCGCCTGCCTTTTTCTTGCCGTCCCATGAAACGTGTGGACAGT
CCCTCGCCCTTTGCCCCCTG

Product: acyl-carrier phosphodiesterase

Products: 4'-phosphopantetheine; apoprotein

Alternate protein names: Azo-dye reductase; FMN-dependent NADH-azo compound oxidoreductase

Number of amino acids: Translated: 210; Mature: 209

Protein sequence:

>210_residues
MSILLVTSSPRGAASHSTRIATEFAEKLLAADPSNTLVVRDLVANPLPHIDADYATGIYTPVEARTPRQAEVVGVSDVVL
DELFAADTVILATGFINFNISSTLKSWVDHIARSGRSFAYGENGPKGLVTGKKVYIVLASGGIYSEGAAVQFDHAIPYLR
GVLGFLGMTDVDVIRIEGVGMGPDAVTAALAKATAKVDAVVASQQAAAAA

Sequences:

>Translated_210_residues
MSILLVTSSPRGAASHSTRIATEFAEKLLAADPSNTLVVRDLVANPLPHIDADYATGIYTPVEARTPRQAEVVGVSDVVL
DELFAADTVILATGFINFNISSTLKSWVDHIARSGRSFAYGENGPKGLVTGKKVYIVLASGGIYSEGAAVQFDHAIPYLR
GVLGFLGMTDVDVIRIEGVGMGPDAVTAALAKATAKVDAVVASQQAAAAA
>Mature_209_residues
SILLVTSSPRGAASHSTRIATEFAEKLLAADPSNTLVVRDLVANPLPHIDADYATGIYTPVEARTPRQAEVVGVSDVVLD
ELFAADTVILATGFINFNISSTLKSWVDHIARSGRSFAYGENGPKGLVTGKKVYIVLASGGIYSEGAAVQFDHAIPYLRG
VLGFLGMTDVDVIRIEGVGMGPDAVTAALAKATAKVDAVVASQQAAAAA

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity

COG id: COG1182

COG function: function code I; Acyl carrier protein phosphodiesterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the azoreductase type 1 family

Homologues:

Organism=Escherichia coli, GI1787680, Length=203, Percent_Identity=38.9162561576355, Blast_Score=149, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AZOR_RHILO (Q98IF8)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_103772.1
- ProteinModelPortal:   Q98IF8
- SMR:   Q98IF8
- GeneID:   1226433
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr2421
- NMPDR:   fig|266835.1.peg.1876
- HOGENOM:   HBG565834
- OMA:   WIDNVAR
- ProtClustDB:   CLSK863494
- HAMAP:   MF_01216
- InterPro:   IPR003680
- InterPro:   IPR023048

Pfam domain/function: PF02525 Flavodoxin_2

EC number: 3.1.4.14

Molecular weight: Translated: 21813; Mature: 21682

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSILLVTSSPRGAASHSTRIATEFAEKLLAADPSNTLVVRDLVANPLPHIDADYATGIYT
CEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEHHHHCCCCCCCCCCCCCCCCC
PVEARTPRQAEVVGVSDVVLDELFAADTVILATGFINFNISSTLKSWVDHIARSGRSFAY
CCCCCCCCCEEEECHHHHHHHHHHHHCEEEEEEEEEEECHHHHHHHHHHHHHHCCCCEEC
GENGPKGLVTGKKVYIVLASGGIYSEGAAVQFDHAIPYLRGVLGFLGMTDVDVIRIEGVG
CCCCCCCCEECCEEEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHCCCCCCEEEEEECCC
MGPDAVTAALAKATAKVDAVVASQQAAAAA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SILLVTSSPRGAASHSTRIATEFAEKLLAADPSNTLVVRDLVANPLPHIDADYATGIYT
EEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEHHHHCCCCCCCCCCCCCCCCC
PVEARTPRQAEVVGVSDVVLDELFAADTVILATGFINFNISSTLKSWVDHIARSGRSFAY
CCCCCCCCCEEEECHHHHHHHHHHHHCEEEEEEEEEEECHHHHHHHHHHHHHHCCCCEEC
GENGPKGLVTGKKVYIVLASGGIYSEGAAVQFDHAIPYLRGVLGFLGMTDVDVIRIEGVG
CCCCCCCCEECCEEEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHCCCCCCEEEEEECCC
MGPDAVTAALAKATAKVDAVVASQQAAAAA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [acyl-carrier protein]; H2O

Specific reaction: [acyl-carrier protein] + H2O = 4'-phosphopantetheine + apoprotein

General reaction: Phosphoric ester hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968