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The map label for this gene is yhjG [H]

Identifier: 13471908

GI number: 13471908

Start: 1667378

End: 1668943

Strand: Direct

Name: yhjG [H]

Synonym: mlr2028

Alternate gene names: 13471908

Gene position: 1667378-1668943 (Clockwise)

Preceding gene: 13471907

Following gene: 13471909

Centisome position: 23.7

GC content: 67.56

Gene sequence:

>1566_bases
ATGCACAGCCACCACCCAATTTCCCATGATGTCGTCATCTCCGGCGCCGGCCCGGTCGGCCTGTTTCTCGCTTGCGAGTT
GCGTTTGGCAGGCCTCTCGGTCCTGATACTGGAAAAGGCCGAAGATCCCCGTTCGCCGCTGAAGCGGCTGCCCTTCGGCA
TGCGCGGTCTCTCGGCACCCACTATAGAGGCCTTCCATCGACGCGACCTGCTGGACGCCATTGCGCCGCCGACGACGAAG
GATGGCTCGGGCAAATTGGTCGCCGCGCACTGGATGCAGCAGCCGCGCCGCCCGGGCGGCCATTTCGCCGGCATCCAGTT
CCATCTCGACGCCGTCGACAGTTCGAAATGGCCCTATCGCCTGCCAGGCCCGGCGGGCACCAGCATGGCGGTCGAGATGG
AGCAGTTGGAAACCATGCTCGCCACCCGCGCGACCGCCATGGGCGTCGAGATCAGGCGCGGCCTTGGCGTCGAAATCTTC
GACCAGTCGGACGAGGACGTGACGATCCGCGCCGGTGGCGAGATTTTTCGCGCCCGCTGGCTCGTCGGTTGCGATGGCGG
CCGCAGCACGGTCCGCAAGCTTGGCGGCTTTGCCTTCACCGGCACGGATGCCGAGTTCACCGGCTATTCCGTTGAGGTCG
AAATAGCCGATCCGGACGCGCTCAGCCCCGGCCGCCACTACACGCCGACGGGCATGTATACCTACTCACGGCCAGGAACC
ATCGCGATGGTCGAGTTCGACGGCGCAACTTTCCACCGGACAGAGCCGATCACACTGGAGCATGTGCAGGCCGTGCTGCG
CCGCGTGTCCGGAACCGACGTCACCCTGACGGCGCTGCGCCTCGCCACGACCTGGACCGATCGCGCCTACCAGGCATCCG
CCTATCGCAACGGGCGGGTGCTGCTCGCCGGCGACGCCGCGCATATCCATTCGCCGCTCGGTGGCCAGGGGCTCAACCTT
GGGATTGGCGATGCGATGAACCTCGGCTGGAAGCTCGCCGCCACCATCCGTGGCGACGCGCCGGCCGGCCTGCTCGACAG
CTATTTCGATGAACGCCATCCGGTGGGAGCGCAAATTCTCGACTGGTCGCGCGCCCAGGTCGCGTTGATGCGGCCAAGCC
GCAGTTCACGCGCGCTCGAAGCCATCATCCGCGACCTCATCGACACGCGCGACGGCGCGACCTACTTCGCCGAGCGCGTC
TGGGGCGTGTCGCTGCGCTACGACGTCGGCGGCAGCCACCCACTGGTGGGCCGCAGCGCGCCCGATTTCGAACTGGCCGA
CGGAACGAGACCCGGCGAACGCTTGAGGACCGGCCAAGCCCTGCTGCTGGACTTCGCTGCCCGCCCTGCCCTCCAGGCGC
TTGCAAGCCGCTGGAGCGGGCGGATCGCCTATGTCGCCGAGGACGCCAGGGAGCGCCTGGGCCTGAGCGCCCTGCTCGTG
CGCCCCGATGGTGTCGTCGCCTGGGCCGCTGATGGTGAGCCTGATCTGGAGGGCGCCGCTCAGGCCGCGTCGCGATGGTT
CGGCGAACCCGGACAGGTGGCTGCGTATGCCAAAGGAACTGTTTGA

Upstream 100 bases:

>100_bases
CCACGACGAACATCGGACGCTGATCGAGCGTCCAATCGTTCGTCCGTGTGGCTCCCAACGCGAGGAACGCTCCGGTCGAA
CATCGACCAAGGAGCAGTTC

Downstream 100 bases:

>100_bases
GCCTGATTTCGGTTGTCTGCTGTGCGAAGTGTCGTTTCACGAAGGCACCTGACGAGCGAACATGAAACATGGCAGACAAG
AAGCGCGATTCCTTGCGAAA

Product: oxygenase

Products: H2O; 3-(2,3-dihydroxyphenyl)propionate; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 521; Mature: 521

Protein sequence:

>521_residues
MHSHHPISHDVVISGAGPVGLFLACELRLAGLSVLILEKAEDPRSPLKRLPFGMRGLSAPTIEAFHRRDLLDAIAPPTTK
DGSGKLVAAHWMQQPRRPGGHFAGIQFHLDAVDSSKWPYRLPGPAGTSMAVEMEQLETMLATRATAMGVEIRRGLGVEIF
DQSDEDVTIRAGGEIFRARWLVGCDGGRSTVRKLGGFAFTGTDAEFTGYSVEVEIADPDALSPGRHYTPTGMYTYSRPGT
IAMVEFDGATFHRTEPITLEHVQAVLRRVSGTDVTLTALRLATTWTDRAYQASAYRNGRVLLAGDAAHIHSPLGGQGLNL
GIGDAMNLGWKLAATIRGDAPAGLLDSYFDERHPVGAQILDWSRAQVALMRPSRSSRALEAIIRDLIDTRDGATYFAERV
WGVSLRYDVGGSHPLVGRSAPDFELADGTRPGERLRTGQALLLDFAARPALQALASRWSGRIAYVAEDARERLGLSALLV
RPDGVVAWAADGEPDLEGAAQAASRWFGEPGQVAAYAKGTV

Sequences:

>Translated_521_residues
MHSHHPISHDVVISGAGPVGLFLACELRLAGLSVLILEKAEDPRSPLKRLPFGMRGLSAPTIEAFHRRDLLDAIAPPTTK
DGSGKLVAAHWMQQPRRPGGHFAGIQFHLDAVDSSKWPYRLPGPAGTSMAVEMEQLETMLATRATAMGVEIRRGLGVEIF
DQSDEDVTIRAGGEIFRARWLVGCDGGRSTVRKLGGFAFTGTDAEFTGYSVEVEIADPDALSPGRHYTPTGMYTYSRPGT
IAMVEFDGATFHRTEPITLEHVQAVLRRVSGTDVTLTALRLATTWTDRAYQASAYRNGRVLLAGDAAHIHSPLGGQGLNL
GIGDAMNLGWKLAATIRGDAPAGLLDSYFDERHPVGAQILDWSRAQVALMRPSRSSRALEAIIRDLIDTRDGATYFAERV
WGVSLRYDVGGSHPLVGRSAPDFELADGTRPGERLRTGQALLLDFAARPALQALASRWSGRIAYVAEDARERLGLSALLV
RPDGVVAWAADGEPDLEGAAQAASRWFGEPGQVAAYAKGTV
>Mature_521_residues
MHSHHPISHDVVISGAGPVGLFLACELRLAGLSVLILEKAEDPRSPLKRLPFGMRGLSAPTIEAFHRRDLLDAIAPPTTK
DGSGKLVAAHWMQQPRRPGGHFAGIQFHLDAVDSSKWPYRLPGPAGTSMAVEMEQLETMLATRATAMGVEIRRGLGVEIF
DQSDEDVTIRAGGEIFRARWLVGCDGGRSTVRKLGGFAFTGTDAEFTGYSVEVEIADPDALSPGRHYTPTGMYTYSRPGT
IAMVEFDGATFHRTEPITLEHVQAVLRRVSGTDVTLTALRLATTWTDRAYQASAYRNGRVLLAGDAAHIHSPLGGQGLNL
GIGDAMNLGWKLAATIRGDAPAGLLDSYFDERHPVGAQILDWSRAQVALMRPSRSSRALEAIIRDLIDTRDGATYFAERV
WGVSLRYDVGGSHPLVGRSAPDFELADGTRPGERLRTGQALLLDFAARPALQALASRWSGRIAYVAEDARERLGLSALLV
RPDGVVAWAADGEPDLEGAAQAASRWFGEPGQVAAYAKGTV

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=368, Percent_Identity=31.25, Blast_Score=119, Evalue=7e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 56143; Mature: 56143

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSHHPISHDVVISGAGPVGLFLACELRLAGLSVLILEKAEDPRSPLKRLPFGMRGLSAP
CCCCCCCCCCEEEECCCCEEEEEEEEEECCCEEEEEEECCCCCHHHHHHCCCCCCCCCCC
TIEAFHRRDLLDAIAPPTTKDGSGKLVAAHWMQQPRRPGGHFAGIQFHLDAVDSSKWPYR
HHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHCCCCCCCCEEEEEEEEEECCCCCCCEE
LPGPAGTSMAVEMEQLETMLATRATAMGVEIRRGLGVEIFDQSDEDVTIRAGGEIFRARW
CCCCCCCCEEEEHHHHHHHHHHHHHHHCHHHHCCCCEEEECCCCCCEEEEECCCEEEEEE
LVGCDGGRSTVRKLGGFAFTGTDAEFTGYSVEVEIADPDALSPGRHYTPTGMYTYSRPGT
EEECCCCHHHHHHHCCEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEECCCCE
IAMVEFDGATFHRTEPITLEHVQAVLRRVSGTDVTLTALRLATTWTDRAYQASAYRNGRV
EEEEEECCCEEECCCCCCHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCEEHHCCCCCEE
LLAGDAAHIHSPLGGQGLNLGIGDAMNLGWKLAATIRGDAPAGLLDSYFDERHPVGAQIL
EEEECHHHHCCCCCCCCCEECCCCHHCCCEEEEEEEECCCCCHHHHHHHHCCCCCCCCEE
DWSRAQVALMRPSRSSRALEAIIRDLIDTRDGATYFAERVWGVSLRYDVGGSHPLVGRSA
CCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCEEEEEECCCCCCCCCCCC
PDFELADGTRPGERLRTGQALLLDFAARPALQALASRWSGRIAYVAEDARERLGLSALLV
CCCCCCCCCCCHHHHHCCCHHHHHHHCCHHHHHHHHHHCCEEEEEEHHHHHHCCCEEEEE
RPDGVVAWAADGEPDLEGAAQAASRWFGEPGQVAAYAKGTV
CCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEECCCC
>Mature Secondary Structure
MHSHHPISHDVVISGAGPVGLFLACELRLAGLSVLILEKAEDPRSPLKRLPFGMRGLSAP
CCCCCCCCCCEEEECCCCEEEEEEEEEECCCEEEEEEECCCCCHHHHHHCCCCCCCCCCC
TIEAFHRRDLLDAIAPPTTKDGSGKLVAAHWMQQPRRPGGHFAGIQFHLDAVDSSKWPYR
HHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHCCCCCCCCEEEEEEEEEECCCCCCCEE
LPGPAGTSMAVEMEQLETMLATRATAMGVEIRRGLGVEIFDQSDEDVTIRAGGEIFRARW
CCCCCCCCEEEEHHHHHHHHHHHHHHHCHHHHCCCCEEEECCCCCCEEEEECCCEEEEEE
LVGCDGGRSTVRKLGGFAFTGTDAEFTGYSVEVEIADPDALSPGRHYTPTGMYTYSRPGT
EEECCCCHHHHHHHCCEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEECCCCE
IAMVEFDGATFHRTEPITLEHVQAVLRRVSGTDVTLTALRLATTWTDRAYQASAYRNGRV
EEEEEECCCEEECCCCCCHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCEEHHCCCCCEE
LLAGDAAHIHSPLGGQGLNLGIGDAMNLGWKLAATIRGDAPAGLLDSYFDERHPVGAQIL
EEEECHHHHCCCCCCCCCEECCCCHHCCCEEEEEEEECCCCCHHHHHHHHCCCCCCCCEE
DWSRAQVALMRPSRSSRALEAIIRDLIDTRDGATYFAERVWGVSLRYDVGGSHPLVGRSA
CCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCEEEEEECCCCCCCCCCCC
PDFELADGTRPGERLRTGQALLLDFAARPALQALASRWSGRIAYVAEDARERLGLSALLV
CCCCCCCCCCCHHHHHCCCHHHHHHHCCHHHHHHHHHHCCEEEEEEHHHHHHCCCEEEEE
RPDGVVAWAADGEPDLEGAAQAASRWFGEPGQVAAYAKGTV
CCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-(3-hydroxyphenyl)propionate; NADH; O2 [C]

Specific reaction: 3-(3-hydroxyphenyl)propionate + NADH + O2 = H2O + 3-(2,3-dihydroxyphenyl)propionate + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]