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The map label for this gene is potB [H]

Identifier: 13471901

GI number: 13471901

Start: 1659295

End: 1660173

Strand: Direct

Name: potB [H]

Synonym: mlr2021

Alternate gene names: 13471901

Gene position: 1659295-1660173 (Clockwise)

Preceding gene: 13471899

Following gene: 13471902

Centisome position: 23.58

GC content: 61.21

Gene sequence:

>879_bases
ATGAAACGACAATGGACATTGTATGATCCACGGCTGGCGTGGCTGCTGGTCGCGCCGGTGCTCCTGCTGCTGATCTTCTT
CCTGGTGCTGCCGATCGCCGTGATGGCCGCCTACACCTTCTTCACCTTCGTCACCGCAGGCGTTGAAACCAGCGCGCTGA
CGACGGCGAACTGGCACGAATTCCTGACCGACAGCTATTATGCCGGCTTCCTTTTGAAGACGCTGCGGGTCGGCGCCATC
ACCGCGCTGCTTTGCGGGGTGCTGGGTTATTTCCCGGCCTATTTCATCTGGGCGACGACCTTCAAGCACAAATGGCTGCT
GCTTCTCCTGCTCATCGTGCCGTTCTGGATCAGCTTCACCATCCGCACCTTTTCGTGGATCAACATCCTGGGCGAACAGG
GCGTCATCAATGTCGCGCTGCTGAAGATGGGCATCATCAGCGAGCCGTTGCCGATGCTCTACAATGAGGGCGCGGTCATC
CTGGGCATGCTGCACTTCCTGCTGCCCTACATGATCCTCAACGTCTATGTCAGCCTCGAGGGCATCGACCGTACCCTGAT
TTCGGCCGCGCGCTCGATGGGTTGCACCAGCGCACAGGCGTTCCGCGAAGTCACGCTTCCGCTTTCGCTGCCGGGCCTGG
CGGCAGGCCTGCTGCTCTGCTTCGTGCTGGCCGCCGGCAGCTATGTCACGCCGCAACTGCTCGGCTCCGGCCGCGACGCG
CTGTTCGGCAATCTGATCTACGACACCATCATGGGCGAGCTGAACTGGCCGATGGGCGCCACGCTTTCGATCGTGCTGTT
CCTCGTGCTCGGCTCCTTCGCCGCCATCTACACACGCTTCATGGGCATGTCGCAGATCGTCAAGGGACTGGGCGGATGA

Upstream 100 bases:

>100_bases
CCGCTGGCTCAATCGCCTGGTGTAGACATCGGGCCACCAATCACCCAATCTTGGCCCCGGGAACAAAACAGGCGCTTCAA
AGAAAGCGCACCGGGTGCTC

Downstream 100 bases:

>100_bases
AGGCGCGGCGGAAACACGAAGGCAGATGGGCCTGGCGGCTGACCGGCTTCGTCACGGTCTGCGTCTACATCTTCATGTTC
GCGCCGATCGTAGCGACGGT

Product: permease protein of ABC transporter

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MKRQWTLYDPRLAWLLVAPVLLLLIFFLVLPIAVMAAYTFFTFVTAGVETSALTTANWHEFLTDSYYAGFLLKTLRVGAI
TALLCGVLGYFPAYFIWATTFKHKWLLLLLLIVPFWISFTIRTFSWINILGEQGVINVALLKMGIISEPLPMLYNEGAVI
LGMLHFLLPYMILNVYVSLEGIDRTLISAARSMGCTSAQAFREVTLPLSLPGLAAGLLLCFVLAAGSYVTPQLLGSGRDA
LFGNLIYDTIMGELNWPMGATLSIVLFLVLGSFAAIYTRFMGMSQIVKGLGG

Sequences:

>Translated_292_residues
MKRQWTLYDPRLAWLLVAPVLLLLIFFLVLPIAVMAAYTFFTFVTAGVETSALTTANWHEFLTDSYYAGFLLKTLRVGAI
TALLCGVLGYFPAYFIWATTFKHKWLLLLLLIVPFWISFTIRTFSWINILGEQGVINVALLKMGIISEPLPMLYNEGAVI
LGMLHFLLPYMILNVYVSLEGIDRTLISAARSMGCTSAQAFREVTLPLSLPGLAAGLLLCFVLAAGSYVTPQLLGSGRDA
LFGNLIYDTIMGELNWPMGATLSIVLFLVLGSFAAIYTRFMGMSQIVKGLGG
>Mature_292_residues
MKRQWTLYDPRLAWLLVAPVLLLLIFFLVLPIAVMAAYTFFTFVTAGVETSALTTANWHEFLTDSYYAGFLLKTLRVGAI
TALLCGVLGYFPAYFIWATTFKHKWLLLLLLIVPFWISFTIRTFSWINILGEQGVINVALLKMGIISEPLPMLYNEGAVI
LGMLHFLLPYMILNVYVSLEGIDRTLISAARSMGCTSAQAFREVTLPLSLPGLAAGLLLCFVLAAGSYVTPQLLGSGRDA
LFGNLIYDTIMGELNWPMGATLSIVLFLVLGSFAAIYTRFMGMSQIVKGLGG

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=256, Percent_Identity=35.546875, Blast_Score=154, Evalue=9e-39,
Organism=Escherichia coli, GI1787080, Length=236, Percent_Identity=32.2033898305085, Blast_Score=137, Evalue=7e-34,
Organism=Escherichia coli, GI1787713, Length=256, Percent_Identity=28.90625, Blast_Score=96, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32206; Mature: 32206

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRQWTLYDPRLAWLLVAPVLLLLIFFLVLPIAVMAAYTFFTFVTAGVETSALTTANWHE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHH
FLTDSYYAGFLLKTLRVGAITALLCGVLGYFPAYFIWATTFKHKWLLLLLLIVPFWISFT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IRTFSWINILGEQGVINVALLKMGIISEPLPMLYNEGAVILGMLHFLLPYMILNVYVSLE
HHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
GIDRTLISAARSMGCTSAQAFREVTLPLSLPGLAAGLLLCFVLAAGSYVTPQLLGSGRDA
HHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHCCCCHH
LFGNLIYDTIMGELNWPMGATLSIVLFLVLGSFAAIYTRFMGMSQIVKGLGG
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKRQWTLYDPRLAWLLVAPVLLLLIFFLVLPIAVMAAYTFFTFVTAGVETSALTTANWHE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHH
FLTDSYYAGFLLKTLRVGAITALLCGVLGYFPAYFIWATTFKHKWLLLLLLIVPFWISFT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IRTFSWINILGEQGVINVALLKMGIISEPLPMLYNEGAVILGMLHFLLPYMILNVYVSLE
HHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
GIDRTLISAARSMGCTSAQAFREVTLPLSLPGLAAGLLLCFVLAAGSYVTPQLLGSGRDA
HHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHCCCCHH
LFGNLIYDTIMGELNWPMGATLSIVLFLVLGSFAAIYTRFMGMSQIVKGLGG
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]