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The map label for this gene is rihB [H]

Identifier: 13471890

GI number: 13471890

Start: 1649930

End: 1650904

Strand: Direct

Name: rihB [H]

Synonym: mlr2009

Alternate gene names: 13471890

Gene position: 1649930-1650904 (Clockwise)

Preceding gene: 13471887

Following gene: 13471892

Centisome position: 23.45

GC content: 65.13

Gene sequence:

>975_bases
ATGGAAAAGATCATCCTGGACTGCGACCCTGGGCACGACGATGCCATCGCCATTCTGCTCGCGGCCGGAAACCCGAAGAT
CGACCTCCTCGGCATCACCACGGTTTCGGGCAACCACAATGTCGAGAACACCACGCGCAATGCGCTTTCCGTGTGCACCG
CCTATGGCATCAAGGTGCCGGTGGCGAAGGGCTCTCCCGGCCCGCTTATCATCGATCAGGTGCTGGCCGTCGAGATTCAC
GGCGATACCGGGCTCGACGGCCCCGTGCTGCCGCCCGCCTCGTTCGAGCTCGACAGGCGGCATGCCGTCGACTTCATCAT
CGACACGGTCATGGCGCATGAGCCGAAGACGGTGACGCTGGTGCCGGTCGGTCCCTACACCAACATCGCGCTTGCCGTGC
GCAAGGACCCGCGCATCGTCGAACGGGTGAAGCGGGTCGTCGCGATGGGCGGCAGTTTTACCCGCGGCAACATCACGCCG
GCGGCGGAGTTCAACGTCTATGCCGACCCGGAAGCCGCCGACGTGGTGTTCCGCGCCGGCTGGGACGTGACCATGGTGGG
GCTCGACCTCACCCACCAGGCGCTGGCCACCCCCGACCTGCAGGATCGCGTGCGCGCCGTCGGCGGCCCGATGGCCAAGT
TCATCCTCGATATCTGGGCGTTCATCGCGACCACGCATGGCGGCCTGCTGCAGATCGAGTTTCCCGCCATCCATGACGCC
TGCTGCGTCGCGGCCATGATCGACGGAAGCGTCTTCACCACCGAGAAGGCCGACATCCGCGTCGAAATTGCCGGCCGGTG
GACCAAGGGGATGACGGTCTGCAATTTCGAGAAGATGGGCGGCATGCGCCATTTCGGCGGCACGGCGAGCGAGCAGACCG
ACTTCCGCCACACCGTGGCGATGAAGCTCGACCATGCGAAGTTCTGTGACCTGATCGTGGACGCGCTGGAGCGGCTAACC
AGGCAGAAGGCGTGA

Upstream 100 bases:

>100_bases
GGGAAGCTGGCTCTGGAGTGGTCGAGCACCAAAACCTTGACTGCCCGCGCGTGCCAGCCTCTTGTGCCCATAGGTTCGGC
CAACAAGGGAGGTGCCCACC

Downstream 100 bases:

>100_bases
TCCAGGCCACCGCTTGGCCCGACCTTCTTTCTTAGCTTTGGTCCAGCTTCGTCCGGATTTCGCCGATGGCATTCACGATC
GCGTCGCCGGCCTGGCGATC

Product: inosine-uridine preferring nucleoside hydrolase

Products: NA

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MEKIILDCDPGHDDAIAILLAAGNPKIDLLGITTVSGNHNVENTTRNALSVCTAYGIKVPVAKGSPGPLIIDQVLAVEIH
GDTGLDGPVLPPASFELDRRHAVDFIIDTVMAHEPKTVTLVPVGPYTNIALAVRKDPRIVERVKRVVAMGGSFTRGNITP
AAEFNVYADPEAADVVFRAGWDVTMVGLDLTHQALATPDLQDRVRAVGGPMAKFILDIWAFIATTHGGLLQIEFPAIHDA
CCVAAMIDGSVFTTEKADIRVEIAGRWTKGMTVCNFEKMGGMRHFGGTASEQTDFRHTVAMKLDHAKFCDLIVDALERLT
RQKA

Sequences:

>Translated_324_residues
MEKIILDCDPGHDDAIAILLAAGNPKIDLLGITTVSGNHNVENTTRNALSVCTAYGIKVPVAKGSPGPLIIDQVLAVEIH
GDTGLDGPVLPPASFELDRRHAVDFIIDTVMAHEPKTVTLVPVGPYTNIALAVRKDPRIVERVKRVVAMGGSFTRGNITP
AAEFNVYADPEAADVVFRAGWDVTMVGLDLTHQALATPDLQDRVRAVGGPMAKFILDIWAFIATTHGGLLQIEFPAIHDA
CCVAAMIDGSVFTTEKADIRVEIAGRWTKGMTVCNFEKMGGMRHFGGTASEQTDFRHTVAMKLDHAKFCDLIVDALERLT
RQKA
>Mature_324_residues
MEKIILDCDPGHDDAIAILLAAGNPKIDLLGITTVSGNHNVENTTRNALSVCTAYGIKVPVAKGSPGPLIIDQVLAVEIH
GDTGLDGPVLPPASFELDRRHAVDFIIDTVMAHEPKTVTLVPVGPYTNIALAVRKDPRIVERVKRVVAMGGSFTRGNITP
AAEFNVYADPEAADVVFRAGWDVTMVGLDLTHQALATPDLQDRVRAVGGPMAKFILDIWAFIATTHGGLLQIEFPAIHDA
CCVAAMIDGSVFTTEKADIRVEIAGRWTKGMTVCNFEKMGGMRHFGGTASEQTDFRHTVAMKLDHAKFCDLIVDALERLT
RQKA

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively. Has a clear preference for cytidine over uridine. Strictly specific for ribonucleosides [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788486, Length=273, Percent_Identity=46.8864468864469, Blast_Score=254, Evalue=5e-69,
Organism=Escherichia coli, GI1786871, Length=319, Percent_Identity=37.6175548589342, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI1786213, Length=319, Percent_Identity=36.3636363636364, Blast_Score=163, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI32565474, Length=189, Percent_Identity=35.4497354497354, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17565698, Length=187, Percent_Identity=31.0160427807487, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6320608, Length=333, Percent_Identity=33.033033033033, Blast_Score=140, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24583915, Length=186, Percent_Identity=32.7956989247312, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24641839, Length=190, Percent_Identity=34.2105263157895, Blast_Score=75, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24641837, Length=185, Percent_Identity=35.1351351351351, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022977 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: =3.2.2.8 [H]

Molecular weight: Translated: 34928; Mature: 34928

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS01247 IUNH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKIILDCDPGHDDAIAILLAAGNPKIDLLGITTVSGNHNVENTTRNALSVCTAYGIKVP
CCEEEEECCCCCCCEEEEEEECCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHCCEEEE
VAKGSPGPLIIDQVLAVEIHGDTGLDGPVLPPASFELDRRHAVDFIIDTVMAHEPKTVTL
ECCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEE
VPVGPYTNIALAVRKDPRIVERVKRVVAMGGSFTRGNITPAAEFNVYADPEAADVVFRAG
EECCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCHHCEEEECC
WDVTMVGLDLTHQALATPDLQDRVRAVGGPMAKFILDIWAFIATTHGGLLQIEFPAIHDA
CCEEEEEECCCHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEECCCHHHH
CCVAAMIDGSVFTTEKADIRVEIAGRWTKGMTVCNFEKMGGMRHFGGTASEQTDFRHTVA
HHHHHHCCCCEEEECCCEEEEEEECCCCCCCEEEEHHHHCCCCCCCCCCCCCCCCCEEEE
MKLDHAKFCDLIVDALERLTRQKA
EEECHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEKIILDCDPGHDDAIAILLAAGNPKIDLLGITTVSGNHNVENTTRNALSVCTAYGIKVP
CCEEEEECCCCCCCEEEEEEECCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHCCEEEE
VAKGSPGPLIIDQVLAVEIHGDTGLDGPVLPPASFELDRRHAVDFIIDTVMAHEPKTVTL
ECCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEE
VPVGPYTNIALAVRKDPRIVERVKRVVAMGGSFTRGNITPAAEFNVYADPEAADVVFRAG
EECCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCHHCEEEECC
WDVTMVGLDLTHQALATPDLQDRVRAVGGPMAKFILDIWAFIATTHGGLLQIEFPAIHDA
CCEEEEEECCCHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEECCCHHHH
CCVAAMIDGSVFTTEKADIRVEIAGRWTKGMTVCNFEKMGGMRHFGGTASEQTDFRHTVA
HHHHHHCCCCEEEECCCEEEEEEECCCCCCCEEEEHHHHCCCCCCCCCCCCCCCCCEEEE
MKLDHAKFCDLIVDALERLTRQKA
EEECHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA