Click here to switch to the map view.

The map label for this gene is paaF [C]

Identifier: 13471764

GI number: 13471764

Start: 1522781

End: 1523596

Strand: Direct

Name: paaF [C]

Synonym: mlr1841

Alternate gene names: 13471764

Gene position: 1522781-1523596 (Clockwise)

Preceding gene: 13471762

Following gene: 13471765

Centisome position: 21.64

GC content: 63.85

Gene sequence:

>816_bases
ATGACCGATGAGACCGTTGCCTTCGATGTCGATGACGGCATCGCCTGGGTGCGCTTCAACCGCCCGGACAAGCGCAATTG
CATGAACCCGGCGCTCAACCGGCGCATGATGGAGGTCCTCGATGAACTCGAGTATCGCGCCGATGTCGGCGTTCTGGTGC
TGTCGGGCGAAGGCAGCGCCTGGTCGGCGGGCATGGACCTCAAGGAGTATTTCCGCGAGACCGAAGCCAAGGGGCTCGGC
GCCGTCCGCAAGGCGCAAGCGGAAGCCTATGGCTGGTGGCGGCGCCTGCGCTGGTACGGCAAGCCGACCATCGCCATGGT
CAATGGCTGGTGTTTCGGCGGCGGCTATGGCCCGCTCTTCGCCTGCGACCTCGCCTTCGCCGCCGACGAGGCGCAGTTCG
CTCTGTCCGAGATCAACTGGGGCATCCTGCCCGGCGGCGGCGCCACCAAGGTGGTGGTCGACCTCCTGTCGATGCGTGAC
GCCATGTACCACGCCCTGACCGGCGAACTCATCGACGGCAGGAAAGCCGCCGCTTGGAAATTGGTCAATGAAAGCCTGCC
GCTGGCGGAGCTGAAGGCGCGGGTCACCGAGGTAGCCAACATCCTCTTGAAGAAGAACCCGATCGCACTGAAGGCGACCA
AGGACGCCATCCGCCGCGTCGGCGAAATGACCTATGACAATGCCGAGGACTATCTGGTGCGGGCGCAGGAGGCGGCCAAC
TCGCACGACAATGAGGGCCGCAAGGAAGGCATTCGCCAGTTCATCGACGAAAAGAGCTACAAGCCAGGTCTCGGCGCTTA
CGACAAGGCCAGATGA

Upstream 100 bases:

>100_bases
AGCGAAAAAGACTATGCGACATAGCAATCGAGATAGCTATGCTCCATAGCAATTTCCATGACTGCGATCATGACTGCGAT
GGAGCGGGAGGAGACCGACC

Downstream 100 bases:

>100_bases
CGCGATAAGCCATCGACGCAATCTGGGAGGAGATGCGCATGCCGGCCTTGACGACTTTAGACCCGGTGGCCCTGCATGCC
GGCGCGCAGCCGGACCGCGT

Product: p-hydroxycinnamoyl CoA hydratase/lyase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MTDETVAFDVDDGIAWVRFNRPDKRNCMNPALNRRMMEVLDELEYRADVGVLVLSGEGSAWSAGMDLKEYFRETEAKGLG
AVRKAQAEAYGWWRRLRWYGKPTIAMVNGWCFGGGYGPLFACDLAFAADEAQFALSEINWGILPGGGATKVVVDLLSMRD
AMYHALTGELIDGRKAAAWKLVNESLPLAELKARVTEVANILLKKNPIALKATKDAIRRVGEMTYDNAEDYLVRAQEAAN
SHDNEGRKEGIRQFIDEKSYKPGLGAYDKAR

Sequences:

>Translated_271_residues
MTDETVAFDVDDGIAWVRFNRPDKRNCMNPALNRRMMEVLDELEYRADVGVLVLSGEGSAWSAGMDLKEYFRETEAKGLG
AVRKAQAEAYGWWRRLRWYGKPTIAMVNGWCFGGGYGPLFACDLAFAADEAQFALSEINWGILPGGGATKVVVDLLSMRD
AMYHALTGELIDGRKAAAWKLVNESLPLAELKARVTEVANILLKKNPIALKATKDAIRRVGEMTYDNAEDYLVRAQEAAN
SHDNEGRKEGIRQFIDEKSYKPGLGAYDKAR
>Mature_270_residues
TDETVAFDVDDGIAWVRFNRPDKRNCMNPALNRRMMEVLDELEYRADVGVLVLSGEGSAWSAGMDLKEYFRETEAKGLGA
VRKAQAEAYGWWRRLRWYGKPTIAMVNGWCFGGGYGPLFACDLAFAADEAQFALSEINWGILPGGGATKVVVDLLSMRDA
MYHALTGELIDGRKAAAWKLVNESLPLAELKARVTEVANILLKKNPIALKATKDAIRRVGEMTYDNAEDYLVRAQEAANS
HDNEGRKEGIRQFIDEKSYKPGLGAYDKAR

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=248, Percent_Identity=27.8225806451613, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI31542718, Length=263, Percent_Identity=26.2357414448669, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI70995211, Length=216, Percent_Identity=26.8518518518519, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI45643119, Length=219, Percent_Identity=28.310502283105, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI260275230, Length=219, Percent_Identity=28.310502283105, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI260274832, Length=219, Percent_Identity=28.310502283105, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI213417737, Length=180, Percent_Identity=27.7777777777778, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1787659, Length=251, Percent_Identity=30.6772908366534, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1788597, Length=210, Percent_Identity=29.5238095238095, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI221142681, Length=232, Percent_Identity=29.7413793103448, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI87082183, Length=261, Percent_Identity=24.904214559387, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790281, Length=179, Percent_Identity=27.9329608938547, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1787660, Length=258, Percent_Identity=25.1937984496124, Blast_Score=64, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17554946, Length=249, Percent_Identity=26.1044176706827, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17540714, Length=215, Percent_Identity=29.7674418604651, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI25145438, Length=253, Percent_Identity=26.8774703557312, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17558304, Length=207, Percent_Identity=27.0531400966184, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17536985, Length=242, Percent_Identity=26.0330578512397, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI25144276, Length=159, Percent_Identity=28.9308176100629, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17508951, Length=159, Percent_Identity=28.9308176100629, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17508953, Length=159, Percent_Identity=28.9308176100629, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17534483, Length=246, Percent_Identity=23.1707317073171, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI20129971, Length=248, Percent_Identity=25.8064516129032, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24653477, Length=248, Percent_Identity=25.8064516129032, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24653139, Length=252, Percent_Identity=26.1904761904762, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19922422, Length=256, Percent_Identity=21.09375, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 30179; Mature: 30047

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDETVAFDVDDGIAWVRFNRPDKRNCMNPALNRRMMEVLDELEYRADVGVLVLSGEGSA
CCCCEEEEECCCCEEEEEECCCCHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCC
WSAGMDLKEYFRETEAKGLGAVRKAQAEAYGWWRRLRWYGKPTIAMVNGWCFGGGYGPLF
CCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECEEECCCCCHHH
ACDLAFAADEAQFALSEINWGILPGGGATKVVVDLLSMRDAMYHALTGELIDGRKAAAWK
HHHHHHHCCHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LVNESLPLAELKARVTEVANILLKKNPIALKATKDAIRRVGEMTYDNAEDYLVRAQEAAN
HHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHCC
SHDNEGRKEGIRQFIDEKSYKPGLGAYDKAR
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TDETVAFDVDDGIAWVRFNRPDKRNCMNPALNRRMMEVLDELEYRADVGVLVLSGEGSA
CCCEEEEECCCCEEEEEECCCCHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCC
WSAGMDLKEYFRETEAKGLGAVRKAQAEAYGWWRRLRWYGKPTIAMVNGWCFGGGYGPLF
CCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECEEECCCCCHHH
ACDLAFAADEAQFALSEINWGILPGGGATKVVVDLLSMRDAMYHALTGELIDGRKAAAWK
HHHHHHHCCHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LVNESLPLAELKARVTEVANILLKKNPIALKATKDAIRRVGEMTYDNAEDYLVRAQEAAN
HHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHCC
SHDNEGRKEGIRQFIDEKSYKPGLGAYDKAR
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA