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The map label for this gene is mdeA [H]

Identifier: 13471598

GI number: 13471598

Start: 1345934

End: 1347217

Strand: Reverse

Name: mdeA [H]

Synonym: mll1618

Alternate gene names: 13471598

Gene position: 1347217-1345934 (Counterclockwise)

Preceding gene: 13471600

Following gene: 13471597

Centisome position: 19.15

GC content: 64.33

Gene sequence:

>1284_bases
ATGACCGCGCCACGCCCGTCGAAAACCCATATCGGCAACCATAAGCTCCATCCGGAGACGCTGATGCTGAGCTATGGCTT
CGATCCGCAGCTTTCGGAAGGCGCGGTCAAGCCGCCGGTGTTCCTGACCTCGACCTTCGTGTTCAAATCGGCAGAAGAGG
GGCGCGATTTCTTCGACTACACGTCCGGCCGCAAGGAACCGCCGAGCGGCACGGCGTCGGGTCTCGTCTATTCACGGTTC
AACCATCCCAACAGCGAGATCGTCGAGGACCGGCTGGCGATCTATGAGGGCACGGATGCCTGCATCCTGTTTTCATCCGG
CATGTCGGCGATCGCCACGACACTGCTCGCCTATGCCCGTCCGGGCGACGTCATACTGCATTCGCAACCGCTCTATGGCG
GCACCGAGACGCTGCTGACACGCACGCTGTCGGGCTTCGGCATCGGCGCTGTCGGCTTCGCCGATGGTGTCGACGAGAAG
GCGGTGCGCGCAGCCGCCGATGCCGCGGTGGCGAAGGGGCGCGTTTCGGTCATCCTGATCGAGACGCCGTCCAATCCGAC
CAACAGCCTGGTCGATATCGCGCTGATGCGGATGATCGCCGACGAGATTGGTGCGAGCCAAGGGTCAGCACCGATCATCG
TCTGCGACAACACGCTGCTCGGCCCGGTGTTCCAGCGGCCGATCGAACATGGCGCCGATGTCTCCGTCTATTCGCTGACC
AAATATGTCGGCGGCCACTCCGACCTGATCGCGGGCGCGGCCATGGGCAGCAAGGCGGTCACCAAGCCGATCAAAGCGCT
GCGCGGCGCGATCGGCACGCAGCTCGACCCGCATTCCTGCTGGATGCTCGGCCGTTCGCTGGAGACGCTGTCAATCCGCA
TGGAGCGGGCCAACGACAATGCGCGCCTGGTCGCGGAATTCCTGCGCGATCATGCCAAGGTCGAGAAGGTGCACTATCTG
CCGTTCCTGGGCGAGGATACGCCGGCGGGGCGCGCCTATGTGAGGCAATGCAGCGGCGCCGGCTCGACCTTCTCCTTCGA
CATCAAAGGCGGAGAGAAGGCGGCTTTCGCGTTTCTCAACGCCCTGCAGATCTTCAAGCTGGCGGTAAGTCTCGGCGGCA
CCGAGTCGCTCGCCAGCCATCCGGCGGCCATGACCCATTCCGGCATTCCCTTCGACGTGCGCCAGCGCATCGGCGTCCTG
GAGACCACGGTCAGGCTGTCGATCGGTGTCGAGCATCCGGACGATCTGATCGCCGACCTGACGCAGGCGCTGGCGGCGGT
CTGA

Upstream 100 bases:

>100_bases
TCTTGCCGGCAAGCAGCCATGCGCGGCCGAAAATAGCAAGGACTGGCCGATCTAGATCGCATAAATTGCGGCACGACATC
CCTCGAAAGCAGGAAAAGCC

Downstream 100 bases:

>100_bases
CGGCTCTCTCGGGACGCCGGAAGGGGCGAGCGATCGCCCTTTCTTCACAAATCCGTGTTCAGGGAAACATTGCGTCTCCC
AGCCGTTGCAGTTGAGGCAT

Product: methionine gamma-lyase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MTAPRPSKTHIGNHKLHPETLMLSYGFDPQLSEGAVKPPVFLTSTFVFKSAEEGRDFFDYTSGRKEPPSGTASGLVYSRF
NHPNSEIVEDRLAIYEGTDACILFSSGMSAIATTLLAYARPGDVILHSQPLYGGTETLLTRTLSGFGIGAVGFADGVDEK
AVRAAADAAVAKGRVSVILIETPSNPTNSLVDIALMRMIADEIGASQGSAPIIVCDNTLLGPVFQRPIEHGADVSVYSLT
KYVGGHSDLIAGAAMGSKAVTKPIKALRGAIGTQLDPHSCWMLGRSLETLSIRMERANDNARLVAEFLRDHAKVEKVHYL
PFLGEDTPAGRAYVRQCSGAGSTFSFDIKGGEKAAFAFLNALQIFKLAVSLGGTESLASHPAAMTHSGIPFDVRQRIGVL
ETTVRLSIGVEHPDDLIADLTQALAAV

Sequences:

>Translated_427_residues
MTAPRPSKTHIGNHKLHPETLMLSYGFDPQLSEGAVKPPVFLTSTFVFKSAEEGRDFFDYTSGRKEPPSGTASGLVYSRF
NHPNSEIVEDRLAIYEGTDACILFSSGMSAIATTLLAYARPGDVILHSQPLYGGTETLLTRTLSGFGIGAVGFADGVDEK
AVRAAADAAVAKGRVSVILIETPSNPTNSLVDIALMRMIADEIGASQGSAPIIVCDNTLLGPVFQRPIEHGADVSVYSLT
KYVGGHSDLIAGAAMGSKAVTKPIKALRGAIGTQLDPHSCWMLGRSLETLSIRMERANDNARLVAEFLRDHAKVEKVHYL
PFLGEDTPAGRAYVRQCSGAGSTFSFDIKGGEKAAFAFLNALQIFKLAVSLGGTESLASHPAAMTHSGIPFDVRQRIGVL
ETTVRLSIGVEHPDDLIADLTQALAAV
>Mature_426_residues
TAPRPSKTHIGNHKLHPETLMLSYGFDPQLSEGAVKPPVFLTSTFVFKSAEEGRDFFDYTSGRKEPPSGTASGLVYSRFN
HPNSEIVEDRLAIYEGTDACILFSSGMSAIATTLLAYARPGDVILHSQPLYGGTETLLTRTLSGFGIGAVGFADGVDEKA
VRAAADAAVAKGRVSVILIETPSNPTNSLVDIALMRMIADEIGASQGSAPIIVCDNTLLGPVFQRPIEHGADVSVYSLTK
YVGGHSDLIAGAAMGSKAVTKPIKALRGAIGTQLDPHSCWMLGRSLETLSIRMERANDNARLVAEFLRDHAKVEKVHYLP
FLGEDTPAGRAYVRQCSGAGSTFSFDIKGGEKAAFAFLNALQIFKLAVSLGGTESLASHPAAMTHSGIPFDVRQRIGVLE
TTVRLSIGVEHPDDLIADLTQALAAV

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=417, Percent_Identity=35.7314148681055, Blast_Score=217, Evalue=2e-56,
Organism=Homo sapiens, GI299473759, Length=417, Percent_Identity=33.0935251798561, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI25453487, Length=417, Percent_Identity=31.1750599520384, Blast_Score=166, Evalue=3e-41,
Organism=Escherichia coli, GI1790375, Length=408, Percent_Identity=35.7843137254902, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1789383, Length=420, Percent_Identity=26.4285714285714, Blast_Score=137, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17538055, Length=418, Percent_Identity=34.688995215311, Blast_Score=203, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI17560056, Length=399, Percent_Identity=34.5864661654135, Blast_Score=200, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI71980708, Length=414, Percent_Identity=30.6763285024155, Blast_Score=177, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6319307, Length=396, Percent_Identity=34.8484848484849, Blast_Score=196, Evalue=7e-51,
Organism=Saccharomyces cerevisiae, GI6323333, Length=434, Percent_Identity=28.3410138248848, Blast_Score=128, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6321904, Length=414, Percent_Identity=28.2608695652174, Blast_Score=123, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6321254, Length=406, Percent_Identity=26.3546798029557, Blast_Score=120, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6321170, Length=350, Percent_Identity=26.2857142857143, Blast_Score=115, Evalue=1e-26,
Organism=Drosophila melanogaster, GI19922554, Length=398, Percent_Identity=36.9346733668342, Blast_Score=229, Evalue=2e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 45384; Mature: 45253

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPRPSKTHIGNHKLHPETLMLSYGFDPQLSEGAVKPPVFLTSTFVFKSAEEGRDFFDY
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEHHHHHHCCHHCCHHHHH
TSGRKEPPSGTASGLVYSRFNHPNSEIVEDRLAIYEGTDACILFSSGMSAIATTLLAYAR
CCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHCC
PGDVILHSQPLYGGTETLLTRTLSGFGIGAVGFADGVDEKAVRAAADAAVAKGRVSVILI
CCCEEEECCCCCCCHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEE
ETPSNPTNSLVDIALMRMIADEIGASQGSAPIIVCDNTLLGPVFQRPIEHGADVSVYSLT
ECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHCCCCEEHHHHH
KYVGGHSDLIAGAAMGSKAVTKPIKALRGAIGTQLDPHSCWMLGRSLETLSIRMERANDN
HHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCEEEEEEEECCCCC
ARLVAEFLRDHAKVEKVHYLPFLGEDTPAGRAYVRQCSGAGSTFSFDIKGGEKAAFAFLN
HHHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCHHHHHHHH
ALQIFKLAVSLGGTESLASHPAAMTHSGIPFDVRQRIGVLETTVRLSIGVEHPDDLIADL
HHHHHHHHHHCCCCHHHHCCCCHHCCCCCCHHHHHHCCHHHEEEEEEECCCCCHHHHHHH
TQALAAV
HHHHHCC
>Mature Secondary Structure 
TAPRPSKTHIGNHKLHPETLMLSYGFDPQLSEGAVKPPVFLTSTFVFKSAEEGRDFFDY
CCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEHHHHHHCCHHCCHHHHH
TSGRKEPPSGTASGLVYSRFNHPNSEIVEDRLAIYEGTDACILFSSGMSAIATTLLAYAR
CCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHCC
PGDVILHSQPLYGGTETLLTRTLSGFGIGAVGFADGVDEKAVRAAADAAVAKGRVSVILI
CCCEEEECCCCCCCHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEE
ETPSNPTNSLVDIALMRMIADEIGASQGSAPIIVCDNTLLGPVFQRPIEHGADVSVYSLT
ECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHCCCCEEHHHHH
KYVGGHSDLIAGAAMGSKAVTKPIKALRGAIGTQLDPHSCWMLGRSLETLSIRMERANDN
HHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCEEEEEEEECCCCC
ARLVAEFLRDHAKVEKVHYLPFLGEDTPAGRAYVRQCSGAGSTFSFDIKGGEKAAFAFLN
HHHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCHHHHHHHH
ALQIFKLAVSLGGTESLASHPAAMTHSGIPFDVRQRIGVLETTVRLSIGVEHPDDLIADL
HHHHHHHHHHCCCCHHHHCCCCHHCCCCCCHHHHHHCCHHHEEEEEEECCCCCHHHHHHH
TQALAAV
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]