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The map label for this gene is metF [H]

Identifier: 13471575

GI number: 13471575

Start: 1325159

End: 1326076

Strand: Reverse

Name: metF [H]

Synonym: mll1587

Alternate gene names: 13471575

Gene position: 1326076-1325159 (Counterclockwise)

Preceding gene: 13471576

Following gene: 13471573

Centisome position: 18.85

GC content: 65.36

Gene sequence:

>918_bases
ATGAACCAGTTCCGCTTTTCCCGCCGCCCCGACATTGGCGACAAGGTCAAGGTTTCCTTCGAATTCTTCCCGCCCAAGAA
TGACGAGATGGAGGCGCGGCTGTGGGACACGGTCACGCGGCTGGAGCCGCTGAAGCCGAAATTCGTCTCGGTGACCTATG
GCGCCGGCGGCTCGACGCGTGAGCGCACGGCGCGCACCGTCGGTCGCATCCTGAACGAGACCAGCCTGACGCCGGCCGCG
CACATGACCTGCGTCGATGCCGCTCGCCATCAGGTCGACGCGGTGATCCAGGAATTCGCCGCTATGGGCGTCAAGCGCTT
CGTCGCCCTGCGCGGCGATCCGGCCGCGGGCGTTGGCACCGCCTACCGCCCGCATCCCGACGGCTATGCCAACGGCGCCG
AACTGGTCGGCGCGATGAAGGACGCGGGCGATTTCGACATTTCGGTTTCGGCTTATCCGGAAAAGCATCCGGAAAGCCCG
GACTTCGCCACCGATATCGACATGCTGAAGCGCAAGGTCGACAATGGCGCGACGCGGGCTATCACCCAGTTCTTCTTCGA
CAATGATCTCTACGAGCGCTATGTCGAGCGTGCGCGCCGGGCGGGCATCTACATCCCGATCGTTCCCGGCATCCTGCCGG
TGCATAATTTCACCCAGGTCGCCAATTTCTCGGCGCGATGCGGCGCGCTGGTGCCGGCATGGCTGGCCGAGCGCTTCGAG
GGCCTGCAGAACGACCCGCAGACCCATGCGCTGGTGGCGTCCGCCGTCGCCGCCGAACAGGTTCTCGACCTGGTCGAACG
CGGGGTGGGCGACTTCCACTTCTACACGATGAACCGCGCCGACCTCGTCTTCGCCATCTGCCACATGATCGGCATCCGCT
CGCACGAGGCGGAGGTGGCAGGTTCGGCTGCGGCGTGA

Upstream 100 bases:

>100_bases
CCGTCAAACTCTGGCTTGGCCGCGACCGGCGCCTGCTGATCGCCGACCCTGCCAATGACAGCCAGCAGACCAAAGTGAAT
TCGATAGGGGAAATCGCCTG

Downstream 100 bases:

>100_bases
GGTTTAATGCATGTCGCCCAAAAGTGGCCCCGGTTTTGGGAGAACGACATGCACGAGAACGAAGACCTAAAGCGCGTCGC
ATGAATCCGTTCAGACGCGA

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MNQFRFSRRPDIGDKVKVSFEFFPPKNDEMEARLWDTVTRLEPLKPKFVSVTYGAGGSTRERTARTVGRILNETSLTPAA
HMTCVDAARHQVDAVIQEFAAMGVKRFVALRGDPAAGVGTAYRPHPDGYANGAELVGAMKDAGDFDISVSAYPEKHPESP
DFATDIDMLKRKVDNGATRAITQFFFDNDLYERYVERARRAGIYIPIVPGILPVHNFTQVANFSARCGALVPAWLAERFE
GLQNDPQTHALVASAVAAEQVLDLVERGVGDFHFYTMNRADLVFAICHMIGIRSHEAEVAGSAAA

Sequences:

>Translated_305_residues
MNQFRFSRRPDIGDKVKVSFEFFPPKNDEMEARLWDTVTRLEPLKPKFVSVTYGAGGSTRERTARTVGRILNETSLTPAA
HMTCVDAARHQVDAVIQEFAAMGVKRFVALRGDPAAGVGTAYRPHPDGYANGAELVGAMKDAGDFDISVSAYPEKHPESP
DFATDIDMLKRKVDNGATRAITQFFFDNDLYERYVERARRAGIYIPIVPGILPVHNFTQVANFSARCGALVPAWLAERFE
GLQNDPQTHALVASAVAAEQVLDLVERGVGDFHFYTMNRADLVFAICHMIGIRSHEAEVAGSAAA
>Mature_305_residues
MNQFRFSRRPDIGDKVKVSFEFFPPKNDEMEARLWDTVTRLEPLKPKFVSVTYGAGGSTRERTARTVGRILNETSLTPAA
HMTCVDAARHQVDAVIQEFAAMGVKRFVALRGDPAAGVGTAYRPHPDGYANGAELVGAMKDAGDFDISVSAYPEKHPESP
DFATDIDMLKRKVDNGATRAITQFFFDNDLYERYVERARRAGIYIPIVPGILPVHNFTQVANFSARCGALVPAWLAERFE
GLQNDPQTHALVASAVAAEQVLDLVERGVGDFHFYTMNRADLVFAICHMIGIRSHEAEVAGSAAA

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=299, Percent_Identity=29.7658862876254, Blast_Score=135, Evalue=5e-32,
Organism=Escherichia coli, GI1790377, Length=283, Percent_Identity=46.9964664310954, Blast_Score=273, Evalue=8e-75,
Organism=Caenorhabditis elegans, GI25147436, Length=289, Percent_Identity=25.6055363321799, Blast_Score=114, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI25147441, Length=289, Percent_Identity=25.6055363321799, Blast_Score=114, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6325234, Length=295, Percent_Identity=30.1694915254237, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6321313, Length=291, Percent_Identity=28.1786941580756, Blast_Score=121, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24662658, Length=269, Percent_Identity=26.3940520446097, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 33577; Mature: 33577

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQFRFSRRPDIGDKVKVSFEFFPPKNDEMEARLWDTVTRLEPLKPKFVSVTYGAGGSTR
CCCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCHH
ERTARTVGRILNETSLTPAAHMTCVDAARHQVDAVIQEFAAMGVKRFVALRGDPAAGVGT
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
AYRPHPDGYANGAELVGAMKDAGDFDISVSAYPEKHPESPDFATDIDMLKRKVDNGATRA
CCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCHHHH
ITQFFFDNDLYERYVERARRAGIYIPIVPGILPVHNFTQVANFSARCGALVPAWLAERFE
HHHHHHCCHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHCCCHHHCHHHHHHHHHHHH
GLQNDPQTHALVASAVAAEQVLDLVERGVGDFHFYTMNRADLVFAICHMIGIRSHEAEVA
HCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHCCCCHHHCC
GSAAA
CCCCC
>Mature Secondary Structure
MNQFRFSRRPDIGDKVKVSFEFFPPKNDEMEARLWDTVTRLEPLKPKFVSVTYGAGGSTR
CCCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCHH
ERTARTVGRILNETSLTPAAHMTCVDAARHQVDAVIQEFAAMGVKRFVALRGDPAAGVGT
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
AYRPHPDGYANGAELVGAMKDAGDFDISVSAYPEKHPESPDFATDIDMLKRKVDNGATRA
CCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCHHHH
ITQFFFDNDLYERYVERARRAGIYIPIVPGILPVHNFTQVANFSARCGALVPAWLAERFE
HHHHHHCCHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHCCCHHHCHHHHHHHHHHHH
GLQNDPQTHALVASAVAAEQVLDLVERGVGDFHFYTMNRADLVFAICHMIGIRSHEAEVA
HCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHCCCCHHHCC
GSAAA
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA