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The map label for this gene is higA-1 [H]

Identifier: 13471567

GI number: 13471567

Start: 1315929

End: 1316237

Strand: Direct

Name: higA-1 [H]

Synonym: mlr1577

Alternate gene names: 13471567

Gene position: 1315929-1316237 (Clockwise)

Preceding gene: 13471566

Following gene: 13471569

Centisome position: 18.7

GC content: 61.81

Gene sequence:

>309_bases
ATGTTGATGACCGCACGCAAGCCAACAACGGTTGGCGAGATTCTCACCGAAGAGTTCATGCAGCCGCTCGGCTTGACGCA
GGCGGCTTTGGCCGAGGCGATGGGCGTTCAGCGCAAGCATGTGAACGAATTGTGCAACGACCGCCGCAACGTGACGGCGG
CAACCGCGCTCATTCTGGCCCGGGTGTTTGGCAACAGCCCGGACTTCTGGCTGAACGTCCAACGCCGCAGCGATCTTTGG
GCGGTTATGAATTCGCCCGACGAGCGGGCACGTGTTGACCGCGCCAAGCCTTTGGCGACAGCCGCATAG

Upstream 100 bases:

>100_bases
GCGTCAACCAGCAGTGGCGACTGATCTTCCGCTGGGACGGCGGTCGCGGTGAAGCGTCGGACATCTATCTCGACGACCAC
AGTTATAAGTGAGCTGAAAC

Downstream 100 bases:

>100_bases
ACAAGATTTCAGGGTAGAGCTTTGTTTTGACGCAATTCCGGACGGAAAACCGCACACACTTTTCCTGGAATTGCTCTAGT
GCAGCGCCTTCACCAGCGCC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 102; Mature: 102

Protein sequence:

>102_residues
MLMTARKPTTVGEILTEEFMQPLGLTQAALAEAMGVQRKHVNELCNDRRNVTAATALILARVFGNSPDFWLNVQRRSDLW
AVMNSPDERARVDRAKPLATAA

Sequences:

>Translated_102_residues
MLMTARKPTTVGEILTEEFMQPLGLTQAALAEAMGVQRKHVNELCNDRRNVTAATALILARVFGNSPDFWLNVQRRSDLW
AVMNSPDERARVDRAKPLATAA
>Mature_102_residues
MLMTARKPTTVGEILTEEFMQPLGLTQAALAEAMGVQRKHVNELCNDRRNVTAATALILARVFGNSPDFWLNVQRRSDLW
AVMNSPDERARVDRAKPLATAA

Specific function: Antitoxin that counteracts the effect of the higB-1 toxin. Binds to its own promoter and regulates transcription of the higB-1/higA-1 operon [H]

COG id: COG3093

COG function: function code R; Plasmid maintenance system antidote protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081745, Length=99, Percent_Identity=41.4141414141414, Blast_Score=78, Evalue=9e-17,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013430
- InterPro:   IPR001387
- InterPro:   IPR010982 [H]

Pfam domain/function: PF01381 HTH_3 [H]

EC number: NA

Molecular weight: Translated: 11336; Mature: 11336

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: PS50943 HTH_CROC1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLMTARKPTTVGEILTEEFMQPLGLTQAALAEAMGVQRKHVNELCNDRRNVTAATALILA
CCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHH
RVFGNSPDFWLNVQRRSDLWAVMNSPDERARVDRAKPLATAA
HHHCCCCCEEEEECCCCCHHHHHCCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MLMTARKPTTVGEILTEEFMQPLGLTQAALAEAMGVQRKHVNELCNDRRNVTAATALILA
CCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHH
RVFGNSPDFWLNVQRRSDLWAVMNSPDERARVDRAKPLATAA
HHHCCCCCEEEEECCCCCHHHHHCCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]