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The map label for this gene is nthA [H]

Identifier: 13471451

GI number: 13471451

Start: 1188468

End: 1189073

Strand: Reverse

Name: nthA [H]

Synonym: mll1425

Alternate gene names: 13471451

Gene position: 1189073-1188468 (Counterclockwise)

Preceding gene: 13471452

Following gene: 13471450

Centisome position: 16.9

GC content: 66.01

Gene sequence:

>606_bases
ATGTCCCATGATCACGACCACGACAACGAACTCGATCCGTTTGCCGCCCGCGTGCGGGCGCTGGAGACCATCCTGACCCG
CAAGGGGCTGATCGATCCGGCAGCGATCGACATCATCGTCGATACTTACGAGACCAAGATCGGCCCGCGCAACGGCGCCA
GGGTGGTGGCCAAGGCTTGGAGCGACCCCGCCTATGCGGATTGGCTCAAGCGCGATGCGACGGCGGCGATCGAGTCGCTG
AGCTATACCGGCCGCCAGGGCGAGCACATGCAGGCGGTGTTCAACACCGAGGAGACGCACAACCTCGTCGTCTGCACGCT
GTGCTCCTGCTATCCATGGTCGGTGCTCGGCCTGCCGCCGGTGTGGTACAAGGCGCCGCCCTACCGGTCGCGAGCGGTGA
TCGATCCGCGCGGCGTGCTCGAGGAATTCGGGCTGACGCTGGCGGCCACCACCAAGATACGCGTCTGGGATTCGACGGCG
GAACTGCGCTATCTCGTCGTGCCGATGCGGCCGGCAGGCACGGAAGGCTGGAGCGAGGAGCAGTTGGCCGAACTGGTGAG
TCGTGACGCGATGATCGGCACGGCGCTGGCGAGAGAGCCGGCATGA

Upstream 100 bases:

>100_bases
CCCAGAAGACGGCCGCCGAATAGCCGGCCACCAGGCGCTATTTCTGCTGGCCCAAGCCGTCATGCTTGCGGCCGGCGGGG
CGGAACCGTAATTTGCGGCC

Downstream 100 bases:

>100_bases
ACGGGCCGCAGGACCTCGGCGGACAGATGGGATTCGGGCCGGTCGCGCCCGAAGAGGACGAGCCCTACTTCCATGCCGAA
TGGGAAAAGCGCGCACTCGG

Product: nitrile hydratase alpha subunit

Products: NA

Alternate protein names: NHase; Nitrilase [H]

Number of amino acids: Translated: 201; Mature: 200

Protein sequence:

>201_residues
MSHDHDHDNELDPFAARVRALETILTRKGLIDPAAIDIIVDTYETKIGPRNGARVVAKAWSDPAYADWLKRDATAAIESL
SYTGRQGEHMQAVFNTEETHNLVVCTLCSCYPWSVLGLPPVWYKAPPYRSRAVIDPRGVLEEFGLTLAATTKIRVWDSTA
ELRYLVVPMRPAGTEGWSEEQLAELVSRDAMIGTALAREPA

Sequences:

>Translated_201_residues
MSHDHDHDNELDPFAARVRALETILTRKGLIDPAAIDIIVDTYETKIGPRNGARVVAKAWSDPAYADWLKRDATAAIESL
SYTGRQGEHMQAVFNTEETHNLVVCTLCSCYPWSVLGLPPVWYKAPPYRSRAVIDPRGVLEEFGLTLAATTKIRVWDSTA
ELRYLVVPMRPAGTEGWSEEQLAELVSRDAMIGTALAREPA
>Mature_200_residues
SHDHDHDNELDPFAARVRALETILTRKGLIDPAAIDIIVDTYETKIGPRNGARVVAKAWSDPAYADWLKRDATAAIESLS
YTGRQGEHMQAVFNTEETHNLVVCTLCSCYPWSVLGLPPVWYKAPPYRSRAVIDPRGVLEEFGLTLAATTKIRVWDSTAE
LRYLVVPMRPAGTEGWSEEQLAELVSRDAMIGTALAREPA

Specific function: NHase catalyzes the hydration of various nitrile compounds to the corresponding amides. Industrial production of acrylamide is now being developed using some of the enzymes of this class [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrile hydratase subunit alpha family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004232
- InterPro:   IPR018141
- ProDom:   PD007559 [H]

Pfam domain/function: PF02979 NHase_alpha [H]

EC number: =4.2.1.84 [H]

Molecular weight: Translated: 22335; Mature: 22203

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHDHDHDNELDPFAARVRALETILTRKGLIDPAAIDIIVDTYETKIGPRNGARVVAKAW
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHEEEEEECHHHCCCCCCCCEEEEECC
SDPAYADWLKRDATAAIESLSYTGRQGEHMQAVFNTEETHNLVVCTLCSCYPWSVLGLPP
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCEEEEEECCCCCCCCCCCCC
VWYKAPPYRSRAVIDPRGVLEEFGLTLAATTKIRVWDSTAELRYLVVPMRPAGTEGWSEE
CCCCCCCCCCCEEECHHHHHHHHCCEEEEEEEEEEECCCCCEEEEEEECCCCCCCCCCHH
QLAELVSRDAMIGTALAREPA
HHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SHDHDHDNELDPFAARVRALETILTRKGLIDPAAIDIIVDTYETKIGPRNGARVVAKAW
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHEEEEEECHHHCCCCCCCCEEEEECC
SDPAYADWLKRDATAAIESLSYTGRQGEHMQAVFNTEETHNLVVCTLCSCYPWSVLGLPP
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCEEEEEECCCCCCCCCCCCC
VWYKAPPYRSRAVIDPRGVLEEFGLTLAATTKIRVWDSTAELRYLVVPMRPAGTEGWSEE
CCCCCCCCCCCEEECHHHHHHHHCCEEEEEEEEEEECCCCCEEEEEEECCCCCCCCCCHH
QLAELVSRDAMIGTALAREPA
HHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9154927 [H]