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The map label for this gene is purN [C]

Identifier: 13471302

GI number: 13471302

Start: 1040099

End: 1040920

Strand: Direct

Name: purN [C]

Synonym: mlr1236

Alternate gene names: 13471302

Gene position: 1040099-1040920 (Clockwise)

Preceding gene: 13471301

Following gene: 13471303

Centisome position: 14.78

GC content: 63.26

Gene sequence:

>822_bases
ATGCGGCAGCCGGCCGCGTGCAAGGCAGGAAAGCGGGCCATGTCTTCACCGGTTGGAACCAGCGCGCCGATCGTCGTCGT
GACCGGCGGCGGCCAGCATGTCTGGGCCATGATCAACGCAATCACCGATCGCGTTGGTCCTGTCAGCGTCATCCTCGAAA
CCCCTGAATCCAAAAAGCAATTGCTGCGTGGGCGAGCCCGCCGCCAGGGTTGGGTCTCGGCCATCGGCCAGCTTGGCACG
ATGGTGCTGAGCAGGTTGGGCAAGCGCCTCCTGGCTGGCCATGGCGCGCGATTGATCGCGGAGGAAAGACTGGAGGTCGA
GCCGCGACCAAGCCAGGAGATCATCCAGGTGGCCTCGGGCAATGGGCCTGAGTGCCTGCAGGCGATCCAGAAGATCCAGC
CAGGCGTCGTGCTGCTCAACGGTTGCCGACTGATCTCGGCCGGTATGCTGAGCAAAATACCCTGCCCCGTGCTCAACTAC
CATGCAGGCATCACGCCGAAATATCGCGGCATGAATGGCGGCTATTGGGCTCTGGTGTCAGGCGATGCGCAGAATTTCGG
CACGACCGTCCATCTTGTCGACGCTGGCGTCGACACGGGCGGCGTGCTGAAGCAGGCGCGCGGCAGGCCGAAAAAAGGCG
ACACCATCTCCAGTCACGCGCTCCGCCAGGCGGCGTTCTCGCGCGACATCTGCGTCGAGGCCGTCAGCGATGCACTGGCC
GGAAAACTCACGACGATCGATCCCGGCCTGCCTTCGAAACAGTGGTATCACCCGACGATCTGGTTCTATGTCTGGACCGG
CCTCAGAACCGGAATCTGGTAG

Upstream 100 bases:

>100_bases
TTCCCGCGCCCTATTACGAATCGAAGCTCGACATCATCGAGTTCAAGCGCTGAGCGTGGTCATCAAGATTTAACCCCATT
CCGGCACAAATCCGGCCAGC

Downstream 100 bases:

>100_bases
GTCTCGTCAGCCTGGAGCCGCACAGGGCCAGACGGCTTTTCGGCACGCCGACCCTGCCGCCGCTGCGCCCCGCGAACGAA
TAAACACGCCTTTCATTTTA

Product: hypothetical protein

Products: N2-Formyl-N1-(5-phospho-D-ribosyl)glycinamide; H+; 5,6,7,8-Tetrahydrofolate [C]

Alternate protein names: Formyl Transferase Domain-Containing Protein; Formyltransferase Protein

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MRQPAACKAGKRAMSSPVGTSAPIVVVTGGGQHVWAMINAITDRVGPVSVILETPESKKQLLRGRARRQGWVSAIGQLGT
MVLSRLGKRLLAGHGARLIAEERLEVEPRPSQEIIQVASGNGPECLQAIQKIQPGVVLLNGCRLISAGMLSKIPCPVLNY
HAGITPKYRGMNGGYWALVSGDAQNFGTTVHLVDAGVDTGGVLKQARGRPKKGDTISSHALRQAAFSRDICVEAVSDALA
GKLTTIDPGLPSKQWYHPTIWFYVWTGLRTGIW

Sequences:

>Translated_273_residues
MRQPAACKAGKRAMSSPVGTSAPIVVVTGGGQHVWAMINAITDRVGPVSVILETPESKKQLLRGRARRQGWVSAIGQLGT
MVLSRLGKRLLAGHGARLIAEERLEVEPRPSQEIIQVASGNGPECLQAIQKIQPGVVLLNGCRLISAGMLSKIPCPVLNY
HAGITPKYRGMNGGYWALVSGDAQNFGTTVHLVDAGVDTGGVLKQARGRPKKGDTISSHALRQAAFSRDICVEAVSDALA
GKLTTIDPGLPSKQWYHPTIWFYVWTGLRTGIW
>Mature_273_residues
MRQPAACKAGKRAMSSPVGTSAPIVVVTGGGQHVWAMINAITDRVGPVSVILETPESKKQLLRGRARRQGWVSAIGQLGT
MVLSRLGKRLLAGHGARLIAEERLEVEPRPSQEIIQVASGNGPECLQAIQKIQPGVVLLNGCRLISAGMLSKIPCPVLNY
HAGITPKYRGMNGGYWALVSGDAQNFGTTVHLVDAGVDTGGVLKQARGRPKKGDTISSHALRQAAFSRDICVEAVSDALA
GKLTTIDPGLPSKQWYHPTIWFYVWTGLRTGIW

Specific function: De novo purine biosynthesis; third step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.1.2.2 [C]

Molecular weight: Translated: 29214; Mature: 29214

Theoretical pI: Translated: 10.60; Mature: 10.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQPAACKAGKRAMSSPVGTSAPIVVVTGGGQHVWAMINAITDRVGPVSVILETPESKKQ
CCCCCCCHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCHHHHH
LLRGRARRQGWVSAIGQLGTMVLSRLGKRLLAGHGARLIAEERLEVEPRPSQEIIQVASG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEHHHHCCCCCCCHHHHHHHHCC
NGPECLQAIQKIQPGVVLLNGCRLISAGMLSKIPCPVLNYHAGITPKYRGMNGGYWALVS
CCHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCEEEEEE
GDAQNFGTTVHLVDAGVDTGGVLKQARGRPKKGDTISSHALRQAAFSRDICVEAVSDALA
CCCCCCCCEEEEEECCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
GKLTTIDPGLPSKQWYHPTIWFYVWTGLRTGIW
CCEEEECCCCCCCCCCCCEEEEEEEHHHHHCCC
>Mature Secondary Structure
MRQPAACKAGKRAMSSPVGTSAPIVVVTGGGQHVWAMINAITDRVGPVSVILETPESKKQ
CCCCCCCHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCHHHHH
LLRGRARRQGWVSAIGQLGTMVLSRLGKRLLAGHGARLIAEERLEVEPRPSQEIIQVASG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEHHHHCCCCCCCHHHHHHHHCC
NGPECLQAIQKIQPGVVLLNGCRLISAGMLSKIPCPVLNYHAGITPKYRGMNGGYWALVS
CCHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCEEEEEE
GDAQNFGTTVHLVDAGVDTGGVLKQARGRPKKGDTISSHALRQAAFSRDICVEAVSDALA
CCCCCCCCEEEEEECCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
GKLTTIDPGLPSKQWYHPTIWFYVWTGLRTGIW
CCEEEECCCCCCCCCCCCEEEEEEEHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 2400 [C]

Specific activity: NA

Km value (mM): 0.15 {N10-formyl-8-deazafolate}} 0.19 {N10-formyl-5-deazafolate}} 0.0167 {N10-formyl-5,8-dideazafolate}} 0.0848 {(6R)-N10-formyltetrahydrofolate}} 0.0775 {(6R)-N10-formyltetrahydrofolate}} 0.6 {N10-formylfolate}} 0.0235 {glycinamide} 0.0192

Substrates: 10-Formyltetrahydrofolate; N1-(5-Phospho-D-ribosyl)glycinamide [C]

Specific reaction: 10-Formyltetrahydrofolate + N1-(5-Phospho-D-ribosyl)glycinamide <==> N2-Formyl-N1-(5-phospho-D-ribosyl)glycinamide + H+ + 5,6,7,8-Tetrahydrofolate [C]

General reaction: Formyl group transfer [C]

Inhibitor: 5, 8-Dideazafolate; N10-(Bromoacetyl)-5, 8-dideazafolate [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA