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The map label for this gene is sigW [H]

Identifier: 13471294

GI number: 13471294

Start: 1032653

End: 1033216

Strand: Reverse

Name: sigW [H]

Synonym: mll1224

Alternate gene names: 13471294

Gene position: 1033216-1032653 (Counterclockwise)

Preceding gene: 13471295

Following gene: 13471293

Centisome position: 14.68

GC content: 65.6

Gene sequence:

>564_bases
ATGATGGCGATGATGCTGGATGAGAGCGAAGCCTCCGACGCCGAACTGATCGGGCGGGCGAAGGGCGGAGACAGGGGGGC
CTTCGGCAAATTGCTGGAGCGCCACTACGACTTCGTCTATCGCGCCGCCTATCGCTGGTGCGGCAAGAAGGCCGATGCCG
AGGACATCGCCCAGGAAGTCTGCGTGCGGCTCGGCAAGGCGATCCGCGACTATCGCGGCGGCGGTGCCTTCACGACATGG
CTCTATTCCGTGACGATGAATGCGGCGCGCGACATGATGCGCAAGACCGCGCGCGAGACGGTGAAGACCGAGGCCTATGG
TGTCCATGCGCTGATTTCGGGCGAGGCCCCGGCCGAACCGGAAGACCCGGTCGAGGCGCTGTGGGCCGCGGTGCGGCAGC
TTCCGGACAAGCAGCGCGACGCCGTCCTGCTGGTCTATGGCGAGGGCTTGAGCCATGCGGCCGCCGCCGAGGCGATGGCG
ATCTCGGAGACGACGGTTTCCTGGCACATCCATGAAGCGAAGAAACGGCTGAAGACGCTGATGCGTTCAGCCGGGGAAGT
GTGA

Upstream 100 bases:

>100_bases
GAGACGCTCGGGTAAGGCCTCTGCAGAAGGAGAGGCCGTTTACCAAAAAAATCTTTGCATCACCCCAAGGATTAGCCATT
AGCCTTCGTCCAACAGGCAA

Downstream 100 bases:

>100_bases
CCATGGTCGACGACAACGAACTCAACAGGCTGCGCGATATCGCGGCACCGGCGCCGGGCGAGAGCGCGAAGGCGCGCGCC
TTCGAAGCCGCCCTGCGCGC

Product: RNA polymerase sigma factor

Products: NA

Alternate protein names: Sigma-W factor [H]

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MMAMMLDESEASDAELIGRAKGGDRGAFGKLLERHYDFVYRAAYRWCGKKADAEDIAQEVCVRLGKAIRDYRGGGAFTTW
LYSVTMNAARDMMRKTARETVKTEAYGVHALISGEAPAEPEDPVEALWAAVRQLPDKQRDAVLLVYGEGLSHAAAAEAMA
ISETTVSWHIHEAKKRLKTLMRSAGEV

Sequences:

>Translated_187_residues
MMAMMLDESEASDAELIGRAKGGDRGAFGKLLERHYDFVYRAAYRWCGKKADAEDIAQEVCVRLGKAIRDYRGGGAFTTW
LYSVTMNAARDMMRKTARETVKTEAYGVHALISGEAPAEPEDPVEALWAAVRQLPDKQRDAVLLVYGEGLSHAAAAEAMA
ISETTVSWHIHEAKKRLKTLMRSAGEV
>Mature_187_residues
MMAMMLDESEASDAELIGRAKGGDRGAFGKLLERHYDFVYRAAYRWCGKKADAEDIAQEVCVRLGKAIRDYRGGGAFTTW
LYSVTMNAARDMMRKTARETVKTEAYGVHALISGEAPAEPEDPVEALWAAVRQLPDKQRDAVLLVYGEGLSHAAAAEAMA
ISETTVSWHIHEAKKRLKTLMRSAGEV

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Sigma-W controls genes involved in transport processes and detoxification [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=188, Percent_Identity=26.063829787234, Blast_Score=66, Evalue=1e-12,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014294
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 20639; Mature: 20639

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMAMMLDESEASDAELIGRAKGGDRGAFGKLLERHYDFVYRAAYRWCGKKADAEDIAQEV
CCEEEECCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
CVRLGKAIRDYRGGGAFTTWLYSVTMNAARDMMRKTARETVKTEAYGVHALISGEAPAEP
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCC
EDPVEALWAAVRQLPDKQRDAVLLVYGEGLSHAAAAEAMAISETTVSWHIHEAKKRLKTL
CCHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MRSAGEV
HHHCCCC
>Mature Secondary Structure
MMAMMLDESEASDAELIGRAKGGDRGAFGKLLERHYDFVYRAAYRWCGKKADAEDIAQEV
CCEEEECCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
CVRLGKAIRDYRGGGAFTTWLYSVTMNAARDMMRKTARETVKTEAYGVHALISGEAPAEP
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCC
EDPVEALWAAVRQLPDKQRDAVLLVYGEGLSHAAAAEAMAISETTVSWHIHEAKKRLKTL
CCHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MRSAGEV
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274029; 9384377; 12207695 [H]