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The map label for this gene is metH [H]

Identifier: 13471290

GI number: 13471290

Start: 1028880

End: 1029902

Strand: Direct

Name: metH [H]

Synonym: mlr1220

Alternate gene names: 13471290

Gene position: 1028880-1029902 (Clockwise)

Preceding gene: 13471289

Following gene: 13471298

Centisome position: 14.62

GC content: 66.76

Gene sequence:

>1023_bases
ATGACCACCACCAATCCGATCGATGCCCTGCTGGCTGAAAAGGGCGTGCTGCTGGCCGATGGCGCCACCGGCACCAATCT
GTTCGCGATGGGTCTGGAGGCCGGCGAGGCGCCGGAGCTGCTGAACGAAACGGCGCCCGACACCATCACCAACCTGCACC
AGAATTTCGTCGATGCCGGCGCCGACATCATCCTGACCAACTCCTTCGGCGGCACCCGCCACCGGCTGAAGCTCCACCAT
GCGCAGGATCGCGTGCATGCGCTCAACAAGCGCGCCGCCGAGATTGCCCGCGCCGTCGCCGACAAGGCCGGCCGCAAGGT
GATCGTCGCCGGCTCCGTCGGCCCGACCGGCGAATTGCTGGTGCCGCTTGGCGCCATGACCTATGACGAGGCGGTCGACG
CCTTTGCCGAGCAGATCGAGGGTCTCAAGCAAGGCGGCGCCGAAGTCGCCTGGATCGAGACCATGTCGGCGCCCGACGAG
ATCCGCGCCGCCGCCGAAGCGGCGATCCGCGTTGGCCTCCCCTACACCTATACCGGCTCCTTCGACACAGCCGGCCGCAC
CATGATGGGCCTGCTGCCGAAGGAAATCCATGGCGTCGTTGACGGGCTGTCCGAAGCACCGCTCGGCGTTGGCGCCAATT
GCGGCGTCGGCGCTTCAGACATCCTGGCCTCGCTGCTCGACATGACCGAGGCGAAGCCGCAGGCGACGGTGATCGTCAAG
GGCAATTGCGGCATTCCGGAATTCCGCGGTACTGAGATCCATTATTCCGGCACGCCGGAACTGATGGCCGACTATGTTCG
CCTCGCCGTCGATGCCGGCGCCAAGATTGTCGGTGGTTGCTGCGGCACTTCGTTCCAGCACCTTGCCGCCATGCGCAAGG
CACTCGACGCCCACACCAAGGCGGGTCGTCCGACGGTCGAGACGATCGTCGAGCGCATCGGTCCGATGCGCAACAAGGTG
GCGACGGAAAACACCGCCGAGACCAGCGAGGCCCGCCGCGAGCGCCGCCGCAGCCGGGCCTGA

Upstream 100 bases:

>100_bases
GCCACGCCATTCGCATTGCCGAAGGTCGCCACAAATCATTGACTAGACATAAAGACTTCTTTATATCCTTATTCGAATTT
GAAACACGAAAGCCAATCCG

Downstream 100 bases:

>100_bases
GGATTTCGATTATTCGCTGTTGTCGGCGTAGCTCGATTGCGCCGACCTGAAATCGACGACATTGTCGCCGCGCATCGTCA
TCAGCGCGCCGGAAGAAGCT

Product: methionine synthase I

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MTTTNPIDALLAEKGVLLADGATGTNLFAMGLEAGEAPELLNETAPDTITNLHQNFVDAGADIILTNSFGGTRHRLKLHH
AQDRVHALNKRAAEIARAVADKAGRKVIVAGSVGPTGELLVPLGAMTYDEAVDAFAEQIEGLKQGGAEVAWIETMSAPDE
IRAAAEAAIRVGLPYTYTGSFDTAGRTMMGLLPKEIHGVVDGLSEAPLGVGANCGVGASDILASLLDMTEAKPQATVIVK
GNCGIPEFRGTEIHYSGTPELMADYVRLAVDAGAKIVGGCCGTSFQHLAAMRKALDAHTKAGRPTVETIVERIGPMRNKV
ATENTAETSEARRERRRSRA

Sequences:

>Translated_340_residues
MTTTNPIDALLAEKGVLLADGATGTNLFAMGLEAGEAPELLNETAPDTITNLHQNFVDAGADIILTNSFGGTRHRLKLHH
AQDRVHALNKRAAEIARAVADKAGRKVIVAGSVGPTGELLVPLGAMTYDEAVDAFAEQIEGLKQGGAEVAWIETMSAPDE
IRAAAEAAIRVGLPYTYTGSFDTAGRTMMGLLPKEIHGVVDGLSEAPLGVGANCGVGASDILASLLDMTEAKPQATVIVK
GNCGIPEFRGTEIHYSGTPELMADYVRLAVDAGAKIVGGCCGTSFQHLAAMRKALDAHTKAGRPTVETIVERIGPMRNKV
ATENTAETSEARRERRRSRA
>Mature_339_residues
TTTNPIDALLAEKGVLLADGATGTNLFAMGLEAGEAPELLNETAPDTITNLHQNFVDAGADIILTNSFGGTRHRLKLHHA
QDRVHALNKRAAEIARAVADKAGRKVIVAGSVGPTGELLVPLGAMTYDEAVDAFAEQIEGLKQGGAEVAWIETMSAPDEI
RAAAEAAIRVGLPYTYTGSFDTAGRTMMGLLPKEIHGVVDGLSEAPLGVGANCGVGASDILASLLDMTEAKPQATVIVKG
NCGIPEFRGTEIHYSGTPELMADYVRLAVDAGAKIVGGCCGTSFQHLAAMRKALDAHTKAGRPTVETIVERIGPMRNKVA
TENTAETSEARRERRRSRA

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG0646

COG function: function code E; Methionine synthase I (cobalamin-dependent), methyltransferase domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=329, Percent_Identity=29.7872340425532, Blast_Score=112, Evalue=5e-25,
Organism=Escherichia coli, GI1790450, Length=280, Percent_Identity=31.0714285714286, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1786456, Length=318, Percent_Identity=27.6729559748428, Blast_Score=73, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17535405, Length=338, Percent_Identity=30.7692307692308, Blast_Score=129, Evalue=3e-30,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 35719; Mature: 35588

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS50970 HCY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTNPIDALLAEKGVLLADGATGTNLFAMGLEAGEAPELLNETAPDTITNLHQNFVDAG
CCCCCHHHHHHHCCCEEEECCCCCCCEEEECCCCCCCHHHHHCCCCHHHHHHHHHHHHCC
ADIILTNSFGGTRHRLKLHHAQDRVHALNKRAAEIARAVADKAGRKVIVAGSVGPTGELL
CCEEEECCCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEE
VPLGAMTYDEAVDAFAEQIEGLKQGGAEVAWIETMSAPDEIRAAAEAAIRVGLPYTYTGS
EECCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHCCCCEEECCC
FDTAGRTMMGLLPKEIHGVVDGLSEAPLGVGANCGVGASDILASLLDMTEAKPQATVIVK
CCCCCHHHHHHCHHHHHHHHHCHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEE
GNCGIPEFRGTEIHYSGTPELMADYVRLAVDAGAKIVGGCCGTSFQHLAAMRKALDAHTK
CCCCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHC
AGRPTVETIVERIGPMRNKVATENTAETSEARRERRRSRA
CCCCHHHHHHHHHCHHHHHHCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTTNPIDALLAEKGVLLADGATGTNLFAMGLEAGEAPELLNETAPDTITNLHQNFVDAG
CCCCHHHHHHHCCCEEEECCCCCCCEEEECCCCCCCHHHHHCCCCHHHHHHHHHHHHCC
ADIILTNSFGGTRHRLKLHHAQDRVHALNKRAAEIARAVADKAGRKVIVAGSVGPTGELL
CCEEEECCCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEE
VPLGAMTYDEAVDAFAEQIEGLKQGGAEVAWIETMSAPDEIRAAAEAAIRVGLPYTYTGS
EECCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHCCCCEEECCC
FDTAGRTMMGLLPKEIHGVVDGLSEAPLGVGANCGVGASDILASLLDMTEAKPQATVIVK
CCCCCHHHHHHCHHHHHHHHHCHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEE
GNCGIPEFRGTEIHYSGTPELMADYVRLAVDAGAKIVGGCCGTSFQHLAAMRKALDAHTK
CCCCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHC
AGRPTVETIVERIGPMRNKVATENTAETSEARRERRRSRA
CCCCHHHHHHHHHCHHHHHHCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]