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The map label for this gene is xthA [H]

Identifier: 13471192

GI number: 13471192

Start: 918211

End: 919005

Strand: Direct

Name: xthA [H]

Synonym: mlr1096

Alternate gene names: 13471192

Gene position: 918211-919005 (Clockwise)

Preceding gene: 13471191

Following gene: 13471193

Centisome position: 13.05

GC content: 63.02

Gene sequence:

>795_bases
ATGAAAATCGTCACCTGGAACATCAACGGCGTTCGCGCCCGCATCGGCAATCTGACCCATTGGCTGACCGAGAGCGCGCC
CGACATTGTCTGCCTGCAGGAGATCAAGACGGTCGACGAGCAGTTCCCGCGCGCCGAGATCGAGGCGCTGGGCTACAATG
TCGAAACCAATGGCCAGAAGGGTTTCAACGGCGTCGCGCTGCTGTCGAAGCTGCGCTTTGACGAAGTCATCAGAGGCCTT
CCGGGCGACGACACCGATGAGCAGGCGCGCTTCATCGAAGGTGTGTTCTCGACCGACAAGGGCGCGCTGCGGGTCGCCTC
TCTCTATCTGCCGAACGGCAATCCGATCGATGACGAGAAGAAATTCCCCTACAAGCTGAACTGGATGGCGCGGCTGGAGC
GCTGGGCCGAAGAGCGGCTGAAGCTGGAAGAGGCGCTGGTGCTGGCCGGCGACTACAACGTCATTCCCGAGCCGATCGAC
GCGCGTTTTCCGGAGAACTGGCTGGGTGACGCGCTGTTCCAGCCGCAGACGCGGCAGGCCTTTCGCCGGCTGCTCAACCT
CGGTTTCACCGAAGCGGTGCGCGCGGTCACCGATGCCCCCGACACCTACACCTTCTGGGATTATCAGGCCGGCGCCTGGC
AGAAGAACAACGGCATCCGCATCGACCATCTGCTGCTGTCGCCGGAGGCCGCCAACCGCTTCTCGTCGGCCTCGATCGAA
AAACATGTGCGCGCCTGGGAAAAGCCGTCCGATCACGTGCCGGTGGCGATCGATCTCGCCTTGCAGCCGGCCTGA

Upstream 100 bases:

>100_bases
CCGCCGGGGGAAAGCGAGTGGATGGCCTTCCTAAAGGACCCGGCAGGCAATACGATCGGCCTCGTCGAACGCCATCCGCC
GCAGGAAGAATAAGACCGTC

Downstream 100 bases:

>100_bases
CCGGGACCCTTCTGCCGGCGAATCGCCCCGACCTTGTATCGCCACAAATCAGGCGCATGATGGAAAATCATCATGGCGGC
TGGGGTTCCCATGACCACTC

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKIVTWNINGVRARIGNLTHWLTESAPDIVCLQEIKTVDEQFPRAEIEALGYNVETNGQKGFNGVALLSKLRFDEVIRGL
PGDDTDEQARFIEGVFSTDKGALRVASLYLPNGNPIDDEKKFPYKLNWMARLERWAEERLKLEEALVLAGDYNVIPEPID
ARFPENWLGDALFQPQTRQAFRRLLNLGFTEAVRAVTDAPDTYTFWDYQAGAWQKNNGIRIDHLLLSPEAANRFSSASIE
KHVRAWEKPSDHVPVAIDLALQPA

Sequences:

>Translated_264_residues
MKIVTWNINGVRARIGNLTHWLTESAPDIVCLQEIKTVDEQFPRAEIEALGYNVETNGQKGFNGVALLSKLRFDEVIRGL
PGDDTDEQARFIEGVFSTDKGALRVASLYLPNGNPIDDEKKFPYKLNWMARLERWAEERLKLEEALVLAGDYNVIPEPID
ARFPENWLGDALFQPQTRQAFRRLLNLGFTEAVRAVTDAPDTYTFWDYQAGAWQKNNGIRIDHLLLSPEAANRFSSASIE
KHVRAWEKPSDHVPVAIDLALQPA
>Mature_264_residues
MKIVTWNINGVRARIGNLTHWLTESAPDIVCLQEIKTVDEQFPRAEIEALGYNVETNGQKGFNGVALLSKLRFDEVIRGL
PGDDTDEQARFIEGVFSTDKGALRVASLYLPNGNPIDDEKKFPYKLNWMARLERWAEERLKLEEALVLAGDYNVIPEPID
ARFPENWLGDALFQPQTRQAFRRLLNLGFTEAVRAVTDAPDTYTFWDYQAGAWQKNNGIRIDHLLLSPEAANRFSSASIE
KHVRAWEKPSDHVPVAIDLALQPA

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction [H]

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=269, Percent_Identity=32.7137546468402, Blast_Score=121, Evalue=6e-28,
Organism=Homo sapiens, GI18375503, Length=269, Percent_Identity=32.7137546468402, Blast_Score=121, Evalue=6e-28,
Organism=Homo sapiens, GI18375501, Length=269, Percent_Identity=32.7137546468402, Blast_Score=121, Evalue=6e-28,
Organism=Escherichia coli, GI1788046, Length=262, Percent_Identity=37.7862595419847, Blast_Score=166, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI71989536, Length=272, Percent_Identity=30.8823529411765, Blast_Score=97, Evalue=7e-21,
Organism=Drosophila melanogaster, GI221330655, Length=275, Percent_Identity=30.5454545454545, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI17136678, Length=275, Percent_Identity=30.5454545454545, Blast_Score=106, Evalue=1e-23,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29917; Mature: 29917

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00726 AP_NUCLEASE_F1_1 ; PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVTWNINGVRARIGNLTHWLTESAPDIVCLQEIKTVDEQFPRAEIEALGYNVETNGQK
CEEEEEECCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHCCHHHHHHHCCCEECCCCC
GFNGVALLSKLRFDEVIRGLPGDDTDEQARFIEGVFSTDKGALRVASLYLPNGNPIDDEK
CCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCC
KFPYKLNWMARLERWAEERLKLEEALVLAGDYNVIPEPIDARFPENWLGDALFQPQTRQA
CCCEEEHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCHHHHHHHCCCHHHHH
FRRLLNLGFTEAVRAVTDAPDTYTFWDYQAGAWQKNNGIRIDHLLLSPEAANRFSSASIE
HHHHHHCCHHHHHHHHHCCCCCEEEEECCCCCEECCCCEEEEEEEECCHHHHHHHHHHHH
KHVRAWEKPSDHVPVAIDLALQPA
HHHHHHCCCCCCCCEEEEEEECCC
>Mature Secondary Structure
MKIVTWNINGVRARIGNLTHWLTESAPDIVCLQEIKTVDEQFPRAEIEALGYNVETNGQK
CEEEEEECCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHCCHHHHHHHCCCEECCCCC
GFNGVALLSKLRFDEVIRGLPGDDTDEQARFIEGVFSTDKGALRVASLYLPNGNPIDDEK
CCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCC
KFPYKLNWMARLERWAEERLKLEEALVLAGDYNVIPEPIDARFPENWLGDALFQPQTRQA
CCCEEEHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCHHHHHHHCCCHHHHH
FRRLLNLGFTEAVRAVTDAPDTYTFWDYQAGAWQKNNGIRIDHLLLSPEAANRFSSASIE
HHHHHHCCHHHHHHHHHCCCCCEEEEECCCCCEECCCCEEEEEEEECCHHHHHHHHHHHH
KHVRAWEKPSDHVPVAIDLALQPA
HHHHHHCCCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]