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The map label for this gene is queA

Identifier: 13470903

GI number: 13470903

Start: 571311

End: 572396

Strand: Reverse

Name: queA

Synonym: mll0724

Alternate gene names: 13470903

Gene position: 572396-571311 (Counterclockwise)

Preceding gene: 13470904

Following gene: 13470902

Centisome position: 8.14

GC content: 64.55

Gene sequence:

>1086_bases
ATGCGCGTCGATCTCTTTGACTTCGAACTGCCGGAGGAGCGCATCGCGCTTCGCCCGGCGGAGCCACGCGACAGCGCCAA
GATGCTGGTGGTTAAATCGGGTGAGGCGCTCGACGACCGCAAAGTCGGCGACTTGCCATCCCTGCTCAGAGCCGGCGACG
TGCTGGTGTTCAATGATACCAAGGTCATTCCGGCGCAGCTCAAAGGCATAAGGCGGCGCGGCGAAGCGCAGGCGCAGGTC
GAGGCTACGCTGCATATGCGCGTGGCGCCGGACCGTTGGCTCGCCTTCATGCGACCCGGCAAGCGCATCGCCGCCGGCGA
CCGCATCCATTTCGGCCATGACGGCAATTCCTGCTTCCTCGGCCAACTTGATGCCACGGTAATAGAGAAGGGCGAGGGCG
GCGAAGCCTTGCTTGGCTTCGACCTGTCGGGGCCGTTCCTCGACGAGGCGCTGCACGCCGTCGGCCACATTCCGCTGCCA
CCCTACATCGCTTCCAAGCGCGACGACGACGAGCGCGATCGGGCCGATTACCAGACGATTTATGCAAGGGAGGAAGGGGC
GGTTGCCGCCCCCACCGCCGGCCTGCATTTCACGCCGGAGCTTTTTGCGGCTCTCGACGCCAAAGGGGTCGAGCGACACT
TCGTGACCTTGCATGTCGGTGCCGGCACGTTCCTGCCGGTCAAGGCGGACGACACCGCCGACCACAAGATGCATGCCGAG
ATCGGCTCGGTCAGCCGGGAAACAGCCGATGCGCTCAACGCGGCCAAGGCGAGGGGCGGGCGCATCATCGCCGTCGGCAC
GACCTCGCTGCGCCTGCTGGAGAGTGCCGCGCGTGAAGACGGTACGGTGCCCGCGTGGTCGGGTCCCACCGACATCTTCA
TCACGCCCGGCTACCGCTTTCGCACCGCCGACATGCTGATGACCAATTTCCATCTGCCGCGCTCGACGCTGTTCATGCTG
GTTTCGGCCTTCAGTGGCCTCGACACGATGGGCGCCGCCTATGCGCATGCCATTGCGAACCGCTACAGGTTCTACTCCTA
TGGAGATGCAAGCCTGCTGTATCGAGCGGAGATGAGCGATGGATGA

Upstream 100 bases:

>100_bases
GCGTCGGTTTCCTCGGCGTCGCGGTGCGTGAAATCTCGCTCGGCCGCGTCGCCGGCGCGGTGATGCTCTTGGCCGGAGCG
CTGCTTATCCGGCTCTACTG

Downstream 100 bases:

>100_bases
TGACCTGCAAACCCTCGATCGCGATGCCCTGATCGCCGAGGTGAAGAAACTGCGCGCCGGCATTCGCGCGCATCGCGACA
CGTCAGGTCATGATCTCTGC

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MRVDLFDFELPEERIALRPAEPRDSAKMLVVKSGEALDDRKVGDLPSLLRAGDVLVFNDTKVIPAQLKGIRRRGEAQAQV
EATLHMRVAPDRWLAFMRPGKRIAAGDRIHFGHDGNSCFLGQLDATVIEKGEGGEALLGFDLSGPFLDEALHAVGHIPLP
PYIASKRDDDERDRADYQTIYAREEGAVAAPTAGLHFTPELFAALDAKGVERHFVTLHVGAGTFLPVKADDTADHKMHAE
IGSVSRETADALNAAKARGGRIIAVGTTSLRLLESAAREDGTVPAWSGPTDIFITPGYRFRTADMLMTNFHLPRSTLFML
VSAFSGLDTMGAAYAHAIANRYRFYSYGDASLLYRAEMSDG

Sequences:

>Translated_361_residues
MRVDLFDFELPEERIALRPAEPRDSAKMLVVKSGEALDDRKVGDLPSLLRAGDVLVFNDTKVIPAQLKGIRRRGEAQAQV
EATLHMRVAPDRWLAFMRPGKRIAAGDRIHFGHDGNSCFLGQLDATVIEKGEGGEALLGFDLSGPFLDEALHAVGHIPLP
PYIASKRDDDERDRADYQTIYAREEGAVAAPTAGLHFTPELFAALDAKGVERHFVTLHVGAGTFLPVKADDTADHKMHAE
IGSVSRETADALNAAKARGGRIIAVGTTSLRLLESAAREDGTVPAWSGPTDIFITPGYRFRTADMLMTNFHLPRSTLFML
VSAFSGLDTMGAAYAHAIANRYRFYSYGDASLLYRAEMSDG
>Mature_361_residues
MRVDLFDFELPEERIALRPAEPRDSAKMLVVKSGEALDDRKVGDLPSLLRAGDVLVFNDTKVIPAQLKGIRRRGEAQAQV
EATLHMRVAPDRWLAFMRPGKRIAAGDRIHFGHDGNSCFLGQLDATVIEKGEGGEALLGFDLSGPFLDEALHAVGHIPLP
PYIASKRDDDERDRADYQTIYAREEGAVAAPTAGLHFTPELFAALDAKGVERHFVTLHVGAGTFLPVKADDTADHKMHAE
IGSVSRETADALNAAKARGGRIIAVGTTSLRLLESAAREDGTVPAWSGPTDIFITPGYRFRTADMLMTNFHLPRSTLFML
VSAFSGLDTMGAAYAHAIANRYRFYSYGDASLLYRAEMSDG

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=356, Percent_Identity=45.5056179775281, Blast_Score=268, Evalue=3e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_RHILO (Q98M55)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_102472.1
- ProteinModelPortal:   Q98M55
- SMR:   Q98M55
- GeneID:   1225135
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll0724
- NMPDR:   fig|266835.1.peg.577
- HOGENOM:   HBG286076
- OMA:   ITRNPAP
- ProtClustDB:   PRK00147
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 39380; Mature: 39380

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVDLFDFELPEERIALRPAEPRDSAKMLVVKSGEALDDRKVGDLPSLLRAGDVLVFNDT
CEEEEECCCCCHHHEEECCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHCCEEEECCC
KVIPAQLKGIRRRGEAQAQVEATLHMRVAPDRWLAFMRPGKRIAAGDRIHFGHDGNSCFL
EECHHHHHHHHHCCCCCEEEEEEEEEEECHHHHHHHHCCCCEEECCCEEEECCCCCEEEE
GQLDATVIEKGEGGEALLGFDLSGPFLDEALHAVGHIPLPPYIASKRDDDERDRADYQTI
EECCEEEEECCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCEEE
YAREEGAVAAPTAGLHFTPELFAALDAKGVERHFVTLHVGAGTFLPVKADDTADHKMHAE
EEECCCCEECCCCCCEECHHHHHHHCCCCCCEEEEEEEECCCEEEEEECCCCCCCHHHHH
IGSVSRETADALNAAKARGGRIIAVGTTSLRLLESAAREDGTVPAWSGPTDIFITPGYRF
HHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCEE
RTADMLMTNFHLPRSTLFMLVSAFSGLDTMGAAYAHAIANRYRFYSYGDASLLYRAEMSD
EHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCEEEEECCCEEEEEEECCC
G
C
>Mature Secondary Structure
MRVDLFDFELPEERIALRPAEPRDSAKMLVVKSGEALDDRKVGDLPSLLRAGDVLVFNDT
CEEEEECCCCCHHHEEECCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHCCEEEECCC
KVIPAQLKGIRRRGEAQAQVEATLHMRVAPDRWLAFMRPGKRIAAGDRIHFGHDGNSCFL
EECHHHHHHHHHCCCCCEEEEEEEEEEECHHHHHHHHCCCCEEECCCEEEECCCCCEEEE
GQLDATVIEKGEGGEALLGFDLSGPFLDEALHAVGHIPLPPYIASKRDDDERDRADYQTI
EECCEEEEECCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCEEE
YAREEGAVAAPTAGLHFTPELFAALDAKGVERHFVTLHVGAGTFLPVKADDTADHKMHAE
EEECCCCEECCCCCCEECHHHHHHHCCCCCCEEEEEEEECCCEEEEEECCCCCCCHHHHH
IGSVSRETADALNAAKARGGRIIAVGTTSLRLLESAAREDGTVPAWSGPTDIFITPGYRF
HHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCEE
RTADMLMTNFHLPRSTLFMLVSAFSGLDTMGAAYAHAIANRYRFYSYGDASLLYRAEMSD
EHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCEEEEECCCEEEEEEECCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968