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The map label for this gene is tpiA

Identifier: 13470811

GI number: 13470811

Start: 476665

End: 477435

Strand: Reverse

Name: tpiA

Synonym: mll0610

Alternate gene names: 13470811

Gene position: 477435-476665 (Counterclockwise)

Preceding gene: 13470818

Following gene: 13470810

Centisome position: 6.79

GC content: 65.89

Gene sequence:

>771_bases
ATGACGCCAGGAATTCGCCCGCTTGTCGCCGGCAACTGGAAAATGAACGGCACCAGCGCTTCGCTGAACGAGCTCAGGAT
GATCGGCAACGGGTTCATGAGCGGGCTCGACGCGGAGACCGAGGCGCTGGTCTGCGTTCCCGCGACGCTGCTTTCCCATG
CAGCCGAGATTCTGTCGCGCACGCCGGTCCATGCGGGCGGCGAGGATTGCCACACCAAGGAAAGCGGCGCCTACACCGGC
TGTATCTCGGCGGAAATGCTGAAGGACGCCGGCGCGAGCCATGTTATTGTCGGCCATTCCGAATGCCGCGAACAACGCGG
CGAGGACGATGCGACGGTCCAGGCCAAGGCCGCCGCCGCATGGCGCGCCGGGCTCGTGGCCATCATTTGCATCGGCGAGA
CGCAGCAGCAGCGTGAGGCGGGCGCCACGCTCGAGGTGCTGTCGCGCCAGGTCGCAGGTTCGGTGCCGCCGAGTGCCACG
CCATCCAACACCGTCATTGCCTATGAGCCGGTGTGGGCGATCGGCACCGGCCTGACGCCGACCGCGGCCGATGTGGCCGA
AGCGCATGCGCATATCCGCGAAAGGCTGTCGGAGAAACTTGGTGCCTCTGCCGCCAAGACGCGCATTCTCTACGGTGGCT
CGGTCAAGCCGTCCAACGCGGTGGAACTGCTGGGCGTCAGGAATGTCGATGGCGCGCTGGTTGGCGGCGCCAGCCTGAAA
GCGGCCGATTTCCTCGGTATCGCCGAGGCCTATCGCAGCATTTCAGGCTGA

Upstream 100 bases:

>100_bases
AAACAAGCGCAATAGCGCCCTTCCGGCCTACTGTCGATTGCTTTGTGGCCTGATTTTGGTAGTGAGGGCCAGAGCTTGAT
ATCGCCCGGAGAAGCAGACC

Downstream 100 bases:

>100_bases
GATTCACGACGAAAGACGGCGCTCATCCCGTCCAAATTCGTTGCAAAGGGCGCGTTGCTTCCCATATTCCGGCCACGGGC
TTGGAAATGCCGTGAAAGCA

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM 1; Triose-phosphate isomerase 1

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MTPGIRPLVAGNWKMNGTSASLNELRMIGNGFMSGLDAETEALVCVPATLLSHAAEILSRTPVHAGGEDCHTKESGAYTG
CISAEMLKDAGASHVIVGHSECREQRGEDDATVQAKAAAAWRAGLVAIICIGETQQQREAGATLEVLSRQVAGSVPPSAT
PSNTVIAYEPVWAIGTGLTPTAADVAEAHAHIRERLSEKLGASAAKTRILYGGSVKPSNAVELLGVRNVDGALVGGASLK
AADFLGIAEAYRSISG

Sequences:

>Translated_256_residues
MTPGIRPLVAGNWKMNGTSASLNELRMIGNGFMSGLDAETEALVCVPATLLSHAAEILSRTPVHAGGEDCHTKESGAYTG
CISAEMLKDAGASHVIVGHSECREQRGEDDATVQAKAAAAWRAGLVAIICIGETQQQREAGATLEVLSRQVAGSVPPSAT
PSNTVIAYEPVWAIGTGLTPTAADVAEAHAHIRERLSEKLGASAAKTRILYGGSVKPSNAVELLGVRNVDGALVGGASLK
AADFLGIAEAYRSISG
>Mature_255_residues
TPGIRPLVAGNWKMNGTSASLNELRMIGNGFMSGLDAETEALVCVPATLLSHAAEILSRTPVHAGGEDCHTKESGAYTGC
ISAEMLKDAGASHVIVGHSECREQRGEDDATVQAKAAAAWRAGLVAIICIGETQQQREAGATLEVLSRQVAGSVPPSATP
SNTVIAYEPVWAIGTGLTPTAADVAEAHAHIRERLSEKLGASAAKTRILYGGSVKPSNAVELLGVRNVDGALVGGASLKA
ADFLGIAEAYRSISG

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI226529917, Length=251, Percent_Identity=42.6294820717131, Blast_Score=163, Evalue=1e-40,
Organism=Homo sapiens, GI4507645, Length=253, Percent_Identity=42.2924901185771, Blast_Score=163, Evalue=1e-40,
Organism=Escherichia coli, GI1790353, Length=247, Percent_Identity=40.4858299595142, Blast_Score=162, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17536593, Length=246, Percent_Identity=43.4959349593496, Blast_Score=177, Evalue=6e-45,
Organism=Saccharomyces cerevisiae, GI6320255, Length=241, Percent_Identity=43.1535269709544, Blast_Score=187, Evalue=2e-48,
Organism=Drosophila melanogaster, GI28572004, Length=245, Percent_Identity=45.3061224489796, Blast_Score=177, Evalue=4e-45,
Organism=Drosophila melanogaster, GI28572008, Length=245, Percent_Identity=45.3061224489796, Blast_Score=177, Evalue=5e-45,
Organism=Drosophila melanogaster, GI28572006, Length=245, Percent_Identity=45.3061224489796, Blast_Score=177, Evalue=5e-45,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS1_RHILO (Q98ME7)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_102380.1
- ProteinModelPortal:   Q98ME7
- SMR:   Q98ME7
- GeneID:   1225043
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll0610
- NMPDR:   fig|266835.1.peg.485
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- ProtClustDB:   PRK00042
- BRENDA:   5.3.1.1
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 26339; Mature: 26208

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 99-99 ACT_SITE 169-169 BINDING 12-12 BINDING 14-14

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPGIRPLVAGNWKMNGTSASLNELRMIGNGFMSGLDAETEALVCVPATLLSHAAEILSR
CCCCCCCEEECCEEECCCCCHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHC
TPVHAGGEDCHTKESGAYTGCISAEMLKDAGASHVIVGHSECREQRGEDDATVQAKAAAA
CCCCCCCCCCCCCCCCCEEEHHHHHHHHHCCCCEEEECCHHHHHHCCCCCCEEEHHHHHH
WRAGLVAIICIGETQQQREAGATLEVLSRQVAGSVPPSATPSNTVIAYEPVWAIGTGLTP
HHCCEEEEEEECCCHHHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEEECCHHEECCCCCC
TAADVAEAHAHIRERLSEKLGASAAKTRILYGGSVKPSNAVELLGVRNVDGALVGGASLK
CHHHHHHHHHHHHHHHHHHHCCHHHHEEEEECCCCCCCCCEEEEEECCCCCEEECCCCCC
AADFLGIAEAYRSISG
HHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TPGIRPLVAGNWKMNGTSASLNELRMIGNGFMSGLDAETEALVCVPATLLSHAAEILSR
CCCCCCEEECCEEECCCCCHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHC
TPVHAGGEDCHTKESGAYTGCISAEMLKDAGASHVIVGHSECREQRGEDDATVQAKAAAA
CCCCCCCCCCCCCCCCCEEEHHHHHHHHHCCCCEEEECCHHHHHHCCCCCCEEEHHHHHH
WRAGLVAIICIGETQQQREAGATLEVLSRQVAGSVPPSATPSNTVIAYEPVWAIGTGLTP
HHCCEEEEEEECCCHHHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEEECCHHEECCCCCC
TAADVAEAHAHIRERLSEKLGASAAKTRILYGGSVKPSNAVELLGVRNVDGALVGGASLK
CHHHHHHHHHHHHHHHHHHHCCHHHHEEEEECCCCCCCCCEEEEEECCCCCEEECCCCCC
AADFLGIAEAYRSISG
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968