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The map label for this gene is dusA [H]

Identifier: 13470773

GI number: 13470773

Start: 415527

End: 416525

Strand: Reverse

Name: dusA [H]

Synonym: mll0566

Alternate gene names: 13470773

Gene position: 416525-415527 (Counterclockwise)

Preceding gene: 13470774

Following gene: 13470770

Centisome position: 5.92

GC content: 63.46

Gene sequence:

>999_bases
ATGTTGAAATATCAAGACCATAGACTGGCTATTGCACCCATGATGGACTGGACGGACCGGCATTGCCGGTTCTTCCATCG
CCAGCTGACGAGCCGTGCACTGCTTTATACCGAGATGGTGGTGGCCGATGCTGTGATCCATGGCGCGCGCGAACGGCTGC
TCGGCTTTGATGACGCGGAACATCCGGTCGCGCTACAGCTCGGCGGCTCGGATCCGGCGAAACTTGCCGAGGCGGCGCGC
ATTGGCGAAGCCTTCGGCTATGACGAGGTCAACCTCAATGTCGGCTGTCCGTCCGATCGCGTCCAATCGGGCACGTTCGG
CGCCTGCCTGATGAAAGTGCCTGACCTCGTCGCCGGTTGCGTCACGGCGATGAAGGCGGCGGTGGCAATACCCGTTACCG
TCAAGTGCCGCATCGGCGTCGACGAGCAGAATCCGGAGCCGGCGCTTGATGCGCTGGCAGATGGGGTTTTCAACGCCGGC
GCCGACGCGCTTTGGGTGCATGCGCGCAAGGCTTGGCTGGAAGGGCTGAGCCCCAAGGAAAACCGCGACATCCCGCCGCT
CGACTATAACAGGGTCTATCGGCTGAAAGCCAAAAAACCGAACGAATTCATTGGTATCAACGGTGGCATTCAATCACTTG
AAGAGGCGCGGGCGCATCTCGATCATGTCGACGGCGCCATGCTCGGCCGCGCCGCCTACCACACGCCAGGCATTCTGGCC
GGCGTTGATGCCGGCTTCTACGGCGCGAAGCCTGAGGCGTTCGATTTCGCCGCGCTGATCGATGCCATGGCGGACTATGC
AGCGCGCCACATCGAACAGGGCGGGCGGCTCGGCCATGTCACCCGCCATATGGTCGGGCTGTTCCATGGGCTGCCCGGTG
CACGCCGCTACCGTCAGATCCTGTCCACCGACGCAAACCGGCCCGGCGCCGGGCCTGACGTGCTGAAGACGGCGTTCGCC
GCCGTCGAATTCAGTGGAGCGGCCGCCGAAGCGGCCTAG

Upstream 100 bases:

>100_bases
GTGCGCCCCGTGCGCGCTGAGAGATCGAAACGTGGCGAAAGGGTCGCATTCTGGCGTAAATTCGTGTGCACTTGGCAGGT
TCTAACAATTTGAAGAAAAA

Downstream 100 bases:

>100_bases
GCTCAGTCCCGCAGGATCATGCGGTCGCCGCGCACGTCGAAGCCTGACAAGGAGCCGATGAAATTCATGCCGAGCAGGCT
CTGGCTCAGCATGCCGGGTG

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MLKYQDHRLAIAPMMDWTDRHCRFFHRQLTSRALLYTEMVVADAVIHGARERLLGFDDAEHPVALQLGGSDPAKLAEAAR
IGEAFGYDEVNLNVGCPSDRVQSGTFGACLMKVPDLVAGCVTAMKAAVAIPVTVKCRIGVDEQNPEPALDALADGVFNAG
ADALWVHARKAWLEGLSPKENRDIPPLDYNRVYRLKAKKPNEFIGINGGIQSLEEARAHLDHVDGAMLGRAAYHTPGILA
GVDAGFYGAKPEAFDFAALIDAMADYAARHIEQGGRLGHVTRHMVGLFHGLPGARRYRQILSTDANRPGAGPDVLKTAFA
AVEFSGAAAEAA

Sequences:

>Translated_332_residues
MLKYQDHRLAIAPMMDWTDRHCRFFHRQLTSRALLYTEMVVADAVIHGARERLLGFDDAEHPVALQLGGSDPAKLAEAAR
IGEAFGYDEVNLNVGCPSDRVQSGTFGACLMKVPDLVAGCVTAMKAAVAIPVTVKCRIGVDEQNPEPALDALADGVFNAG
ADALWVHARKAWLEGLSPKENRDIPPLDYNRVYRLKAKKPNEFIGINGGIQSLEEARAHLDHVDGAMLGRAAYHTPGILA
GVDAGFYGAKPEAFDFAALIDAMADYAARHIEQGGRLGHVTRHMVGLFHGLPGARRYRQILSTDANRPGAGPDVLKTAFA
AVEFSGAAAEAA
>Mature_332_residues
MLKYQDHRLAIAPMMDWTDRHCRFFHRQLTSRALLYTEMVVADAVIHGARERLLGFDDAEHPVALQLGGSDPAKLAEAAR
IGEAFGYDEVNLNVGCPSDRVQSGTFGACLMKVPDLVAGCVTAMKAAVAIPVTVKCRIGVDEQNPEPALDALADGVFNAG
ADALWVHARKAWLEGLSPKENRDIPPLDYNRVYRLKAKKPNEFIGINGGIQSLEEARAHLDHVDGAMLGRAAYHTPGILA
GVDAGFYGAKPEAFDFAALIDAMADYAARHIEQGGRLGHVTRHMVGLFHGLPGARRYRQILSTDANRPGAGPDVLKTAFA
AVEFSGAAAEAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=239, Percent_Identity=28.4518828451883, Blast_Score=72, Evalue=5e-13,
Organism=Escherichia coli, GI145693211, Length=316, Percent_Identity=56.9620253164557, Blast_Score=366, Evalue=1e-102,
Organism=Escherichia coli, GI1789660, Length=244, Percent_Identity=29.9180327868852, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI1788462, Length=180, Percent_Identity=32.2222222222222, Blast_Score=67, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323560, Length=237, Percent_Identity=27.0042194092827, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24580595, Length=304, Percent_Identity=25.9868421052632, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19920448, Length=304, Percent_Identity=25.9868421052632, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 35746; Mature: 35746

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKYQDHRLAIAPMMDWTDRHCRFFHRQLTSRALLYTEMVVADAVIHGARERLLGFDDAE
CCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
HPVALQLGGSDPAKLAEAARIGEAFGYDEVNLNVGCPSDRVQSGTFGACLMKVPDLVAGC
CCEEEEECCCCHHHHHHHHHHHHHCCCCCEEEECCCCHHHCCCCCHHHHHHHHHHHHHHH
VTAMKAAVAIPVTVKCRIGVDEQNPEPALDALADGVFNAGADALWVHARKAWLEGLSPKE
HHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCC
NRDIPPLDYNRVYRLKAKKPNEFIGINGGIQSLEEARAHLDHVDGAMLGRAAYHTPGILA
CCCCCCCCCCCEEEEECCCCCCEEECCCCHHHHHHHHHHHHHHCHHHHHHHHHCCCCEEE
GVDAGFYGAKPEAFDFAALIDAMADYAARHIEQGGRLGHVTRHMVGLFHGLPGARRYRQI
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHH
LSTDANRPGAGPDVLKTAFAAVEFSGAAAEAA
HHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MLKYQDHRLAIAPMMDWTDRHCRFFHRQLTSRALLYTEMVVADAVIHGARERLLGFDDAE
CCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
HPVALQLGGSDPAKLAEAARIGEAFGYDEVNLNVGCPSDRVQSGTFGACLMKVPDLVAGC
CCEEEEECCCCHHHHHHHHHHHHHCCCCCEEEECCCCHHHCCCCCHHHHHHHHHHHHHHH
VTAMKAAVAIPVTVKCRIGVDEQNPEPALDALADGVFNAGADALWVHARKAWLEGLSPKE
HHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCC
NRDIPPLDYNRVYRLKAKKPNEFIGINGGIQSLEEARAHLDHVDGAMLGRAAYHTPGILA
CCCCCCCCCCCEEEEECCCCCCEEECCCCHHHHHHHHHHHHHHCHHHHHHHHHCCCCEEE
GVDAGFYGAKPEAFDFAALIDAMADYAARHIEQGGRLGHVTRHMVGLFHGLPGARRYRQI
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHH
LSTDANRPGAGPDVLKTAFAAVEFSGAAAEAA
HHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]