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The map label for this gene is glnA [H]

Identifier: 13470589

GI number: 13470589

Start: 238290

End: 239699

Strand: Reverse

Name: glnA [H]

Synonym: mll0343

Alternate gene names: 13470589

Gene position: 239699-238290 (Counterclockwise)

Preceding gene: 13470590

Following gene: 13470585

Centisome position: 3.41

GC content: 61.13

Gene sequence:

>1410_bases
ATGACGACAGCCAAAGACATCATGAAGCAGATCAAGGACAACGACGTGAAATTCGTCGACCTGCGCTTCACCGACCCGAA
GGGCAAGCTGCAGCACGTAACGATGGATGTCGTCGAGGTCGAGGAAGACATGTTCGCCGATGGTGTCATGTTCGACGGCT
CCTCGATTGCCGGCTGGAAGGCCATCAACGAGTCCGACATGGTGCTCATGCCCGATCCGGACACGGTCCACATGGATCCG
TTCTTCGCGCAGTCGACCATGGTCATCCTGTGCGACATCCTCGATCCGGTCTCGGGCGAATCCTACAACCGCGACCCGCG
CGGCACGGCCAAGAAGGCCGAAGCCTACATGAAGTCGGAAGGCATCGGCGACCAGATCTTCGTCGGCCCGGAAGCCGAAT
TCTTCGTCTTCGATGACGTCAAGTACAAGGCCGACCCGTACAACACCGGCTTCAAGCTCGACTCCACCGAACTGCCGTCA
AACGACGACACCGACTACGAGACCGGCAATCTCGGCCATCGCCCGCGCATCAAGGGCGGCTATTTCCCGGTGCCGCCGAT
CGACAGCGCGCAGGACATGCGCTCCGAGATGCTGACCGTGCTCGCGGAAATGGGCGTGCGCGTCGAAAAGCATCACCACG
AAGTCGCCGCCGCGCAGCACGAACTCGGCATCAAGTTCGACACGCTGGTCCGCAACGCCGACAAGATGCTGATCTACAAG
TATGTCGTGCACCAGGTCGCCAACGCCTATGGCAAGACGGCCACCTTCATGCCGAAGCCCATCTTCGGCGACAATGGCTC
GGGCATGCACGTCCACCAGTCGATCTGGAAGGGCGGCAAGCCGACCTTCGCCGGCAATGAATATGCCGGCCTGTCGGAAA
GCTGCCTGTTCTACATCGGCGGCATCATCAAGCACGCCAAGGCGATCAACGCGTTCACCAACCCGCTGACGAACTCCTAC
AAGCGCCTGGTGCCCGGCTATGAAGCGCCGGTGCTGCTCGCCTATTCCGCGCGCAACCGTTCGGCCTCCTGCCGCATCCC
GTTCGGCTCGTCGCCGAAGGCCAAGCGCGTCGAGGTCCGCTTCCCCGATCCGGGCGCGAACCCGTACCTCGCCTTCGCAG
CCATGCTGATGGCCGGCCTCGACGGCATCAAGAACAAGATCCACCCCGGCCAGCCGATGGACAAGGATCTCTACGACCTG
CCGCCGAAGGAGTTGAAGAAGATCCCGACCGTCTGCGGCTCGCTGCGTGAAGCTCTCCAGAGCCTCGATAAGGATCGCGG
CTTCCTGAAGGCCGGCGGCGTCTTCGACGACGACCAGATCGACAGCTACATCGAACTGAAGATGGCCGAGGTGATGCGCT
TTGAAATGACCCCGCATCCGGTGGAATACGACATGTACTATTCGGTGTAA

Upstream 100 bases:

>100_bases
CGCATCCGCACCGGTGAGACCGGAATGGACGCGGTCTGACGCGACCATCCTCCAAAAAACGTCATCCATCAAAAAACGTC
ATCAAACAGGGATACATGAC

Downstream 100 bases:

>100_bases
GAACCCGTACTGATAACATTGAAAAGGCCCTTGAAGGATTTCCCCCTTCAAGGGCCTTTTGCTGTTCGTCAATGCTCATC
GATGGCCGCGCGCGTGGCCA

Product: glutamine synthetase I

Products: NA

Alternate protein names: Glutamate--ammonia ligase I; Glutamine synthetase I; GSI [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MTTAKDIMKQIKDNDVKFVDLRFTDPKGKLQHVTMDVVEVEEDMFADGVMFDGSSIAGWKAINESDMVLMPDPDTVHMDP
FFAQSTMVILCDILDPVSGESYNRDPRGTAKKAEAYMKSEGIGDQIFVGPEAEFFVFDDVKYKADPYNTGFKLDSTELPS
NDDTDYETGNLGHRPRIKGGYFPVPPIDSAQDMRSEMLTVLAEMGVRVEKHHHEVAAAQHELGIKFDTLVRNADKMLIYK
YVVHQVANAYGKTATFMPKPIFGDNGSGMHVHQSIWKGGKPTFAGNEYAGLSESCLFYIGGIIKHAKAINAFTNPLTNSY
KRLVPGYEAPVLLAYSARNRSASCRIPFGSSPKAKRVEVRFPDPGANPYLAFAAMLMAGLDGIKNKIHPGQPMDKDLYDL
PPKELKKIPTVCGSLREALQSLDKDRGFLKAGGVFDDDQIDSYIELKMAEVMRFEMTPHPVEYDMYYSV

Sequences:

>Translated_469_residues
MTTAKDIMKQIKDNDVKFVDLRFTDPKGKLQHVTMDVVEVEEDMFADGVMFDGSSIAGWKAINESDMVLMPDPDTVHMDP
FFAQSTMVILCDILDPVSGESYNRDPRGTAKKAEAYMKSEGIGDQIFVGPEAEFFVFDDVKYKADPYNTGFKLDSTELPS
NDDTDYETGNLGHRPRIKGGYFPVPPIDSAQDMRSEMLTVLAEMGVRVEKHHHEVAAAQHELGIKFDTLVRNADKMLIYK
YVVHQVANAYGKTATFMPKPIFGDNGSGMHVHQSIWKGGKPTFAGNEYAGLSESCLFYIGGIIKHAKAINAFTNPLTNSY
KRLVPGYEAPVLLAYSARNRSASCRIPFGSSPKAKRVEVRFPDPGANPYLAFAAMLMAGLDGIKNKIHPGQPMDKDLYDL
PPKELKKIPTVCGSLREALQSLDKDRGFLKAGGVFDDDQIDSYIELKMAEVMRFEMTPHPVEYDMYYSV
>Mature_468_residues
TTAKDIMKQIKDNDVKFVDLRFTDPKGKLQHVTMDVVEVEEDMFADGVMFDGSSIAGWKAINESDMVLMPDPDTVHMDPF
FAQSTMVILCDILDPVSGESYNRDPRGTAKKAEAYMKSEGIGDQIFVGPEAEFFVFDDVKYKADPYNTGFKLDSTELPSN
DDTDYETGNLGHRPRIKGGYFPVPPIDSAQDMRSEMLTVLAEMGVRVEKHHHEVAAAQHELGIKFDTLVRNADKMLIYKY
VVHQVANAYGKTATFMPKPIFGDNGSGMHVHQSIWKGGKPTFAGNEYAGLSESCLFYIGGIIKHAKAINAFTNPLTNSYK
RLVPGYEAPVLLAYSARNRSASCRIPFGSSPKAKRVEVRFPDPGANPYLAFAAMLMAGLDGIKNKIHPGQPMDKDLYDLP
PKELKKIPTVCGSLREALQSLDKDRGFLKAGGVFDDDQIDSYIELKMAEVMRFEMTPHPVEYDMYYSV

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=468, Percent_Identity=64.1025641025641, Blast_Score=637, Evalue=0.0,
Organism=Escherichia coli, GI87081870, Length=392, Percent_Identity=23.7244897959184, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 52255; Mature: 52124

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTAKDIMKQIKDNDVKFVDLRFTDPKGKLQHVTMDVVEVEEDMFADGVMFDGSSIAGWK
CCCHHHHHHHHCCCCEEEEEEEEECCCCCEEEEEHHHHHHHHHHHHCCEEECCCCCCCCE
AINESDMVLMPDPDTVHMDPFFAQSTMVILCDILDPVSGESYNRDPRGTAKKAEAYMKSE
ECCCCCEEEECCCCEEECCCHHHCCHHEEEEEHHCCCCCCCCCCCCCCHHHHHHHHHHHC
GIGDQIFVGPEAEFFVFDDVKYKADPYNTGFKLDSTELPSNDDTDYETGNLGHRPRIKGG
CCCCEEEECCCCCEEEEECCEEECCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCC
YFPVPPIDSAQDMRSEMLTVLAEMGVRVEKHHHEVAAAQHELGIKFDTLVRNADKMLIYK
CCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHH
YVVHQVANAYGKTATFMPKPIFGDNGSGMHVHQSIWKGGKPTFAGNEYAGLSESCLFYIG
HHHHHHHHHHCCCEECCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHH
GIIKHAKAINAFTNPLTNSYKRLVPGYEAPVLLAYSARNRSASCRIPFGSSPKAKRVEVR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEECCCCCCCCCCEEEEE
FPDPGANPYLAFAAMLMAGLDGIKNKIHPGQPMDKDLYDLPPKELKKIPTVCGSLREALQ
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHH
SLDKDRGFLKAGGVFDDDQIDSYIELKMAEVMRFEMTPHPVEYDMYYSV
HHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECC
>Mature Secondary Structure 
TTAKDIMKQIKDNDVKFVDLRFTDPKGKLQHVTMDVVEVEEDMFADGVMFDGSSIAGWK
CCHHHHHHHHCCCCEEEEEEEEECCCCCEEEEEHHHHHHHHHHHHCCEEECCCCCCCCE
AINESDMVLMPDPDTVHMDPFFAQSTMVILCDILDPVSGESYNRDPRGTAKKAEAYMKSE
ECCCCCEEEECCCCEEECCCHHHCCHHEEEEEHHCCCCCCCCCCCCCCHHHHHHHHHHHC
GIGDQIFVGPEAEFFVFDDVKYKADPYNTGFKLDSTELPSNDDTDYETGNLGHRPRIKGG
CCCCEEEECCCCCEEEEECCEEECCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCC
YFPVPPIDSAQDMRSEMLTVLAEMGVRVEKHHHEVAAAQHELGIKFDTLVRNADKMLIYK
CCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHH
YVVHQVANAYGKTATFMPKPIFGDNGSGMHVHQSIWKGGKPTFAGNEYAGLSESCLFYIG
HHHHHHHHHHCCCEECCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHH
GIIKHAKAINAFTNPLTNSYKRLVPGYEAPVLLAYSARNRSASCRIPFGSSPKAKRVEVR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEECCCCCCCCCCEEEEE
FPDPGANPYLAFAAMLMAGLDGIKNKIHPGQPMDKDLYDLPPKELKKIPTVCGSLREALQ
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHH
SLDKDRGFLKAGGVFDDDQIDSYIELKMAEVMRFEMTPHPVEYDMYYSV
HHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2882467 [H]