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The map label for this gene is rpoA

Identifier: 13470575

GI number: 13470575

Start: 222276

End: 223286

Strand: Direct

Name: rpoA

Synonym: mlr0325

Alternate gene names: 13470575

Gene position: 222276-223286 (Clockwise)

Preceding gene: 13470574

Following gene: 13470576

Centisome position: 3.16

GC content: 61.23

Gene sequence:

>1011_bases
ATGATCCAGAAAAATTGGCAGGAACTGATCAAGCCGAACAAGATCGAGTTCTCGTCGAAGAAGAAGACGCTGACCACGCT
GGTCGCCGAGCCGCTCGAGCGTGGCTTTGGCCTCACCCTCGGCAACGCGCTGCGCCGCGTGCTTCTGTCTTCGCTGCGCG
GTGCGGCTGTGACCGCTGTGCAGATCGACGGCGTTCTGCATGAATTCTCGTCTATCGCCGGCGTGCGCGAGGACGTGACC
GACATCGTCTTGAACATCAAGGAGATCGCCATCCGCATGGAAGGCGATGGCCCCAAGCGCATGGTCGTGCGCAAGCAGGG
ACCGGGTGCGGTTCTCGCCGGCGACATCCAGACGGTTGGTGACGTTGAGATCCTCAACCCCGACCACGTCATCTGCACGC
TGGACGAAGGCGCTGAAATCCGCATGGAATTCACCGTCGACACCGGCAAGGGTTACGTGCCGGCCGAGCGCAACCGCGCC
GAGGACGCGCCGATTGGCCTGATCCCGGTCGACAGCCTCTATTCGCCCGTCAAGAAGGTCTCCTACAAGGTCGAAAACAC
CCGCGAGGGCCAGGTTCTCGACTATGACAAGCTGACCATGACGATCGAGACCGACGGTTCGATCTCGGGCGAGGACGCAG
TGGCCTTCGCCGCCCGCATCCTGCAGGACCAGCTCGGCCTGTTCGTCAACTTCGACGAGCCGCAGAAGGAAGTCGCCGCC
GAAGCCGTCACCGAGCTCGCCTTCAACCCGGCGCTGCTCAAGAAGGTCGACGAACTTGAACTTTCGGTGCGTTCGGCCAA
CTGCCTGAAGAACGACAACATCGTCTACATCGGCGATCTCATCCAGAAGACCGAAGCCGAGATGCTGCGCACCCCGAACT
TCGGCCGCAAGTCGCTGAACGAGATCAAGGAAGTCCTGGCGGCGATGGGCCTGCACCTCGGCATGGAAGTGCCGGATTGG
CCGCCGGAAAACATCGAAGACCTCGCCAAGCGTTACGAAGACCAATATTGA

Upstream 100 bases:

>100_bases
TTACCGAACGCCGCGCGAACGCTCACAGGGGCGTTCCTCCGCGGTATTCCAGGTCGCCCGCCACGATTGGATGGTGGCGG
GTCAACGGAAGGAAAGCATC

Downstream 100 bases:

>100_bases
GCATGAATTCCAAGGATCGGCGGCGTGGGCCGCTCGATCCGACGGTCATGCGGAAAACCATCAGAGGCCTTGGCCTCTTG
AACAACTAAAGAAGGAAAAG

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MIQKNWQELIKPNKIEFSSKKKTLTTLVAEPLERGFGLTLGNALRRVLLSSLRGAAVTAVQIDGVLHEFSSIAGVREDVT
DIVLNIKEIAIRMEGDGPKRMVVRKQGPGAVLAGDIQTVGDVEILNPDHVICTLDEGAEIRMEFTVDTGKGYVPAERNRA
EDAPIGLIPVDSLYSPVKKVSYKVENTREGQVLDYDKLTMTIETDGSISGEDAVAFAARILQDQLGLFVNFDEPQKEVAA
EAVTELAFNPALLKKVDELELSVRSANCLKNDNIVYIGDLIQKTEAEMLRTPNFGRKSLNEIKEVLAAMGLHLGMEVPDW
PPENIEDLAKRYEDQY

Sequences:

>Translated_336_residues
MIQKNWQELIKPNKIEFSSKKKTLTTLVAEPLERGFGLTLGNALRRVLLSSLRGAAVTAVQIDGVLHEFSSIAGVREDVT
DIVLNIKEIAIRMEGDGPKRMVVRKQGPGAVLAGDIQTVGDVEILNPDHVICTLDEGAEIRMEFTVDTGKGYVPAERNRA
EDAPIGLIPVDSLYSPVKKVSYKVENTREGQVLDYDKLTMTIETDGSISGEDAVAFAARILQDQLGLFVNFDEPQKEVAA
EAVTELAFNPALLKKVDELELSVRSANCLKNDNIVYIGDLIQKTEAEMLRTPNFGRKSLNEIKEVLAAMGLHLGMEVPDW
PPENIEDLAKRYEDQY
>Mature_336_residues
MIQKNWQELIKPNKIEFSSKKKTLTTLVAEPLERGFGLTLGNALRRVLLSSLRGAAVTAVQIDGVLHEFSSIAGVREDVT
DIVLNIKEIAIRMEGDGPKRMVVRKQGPGAVLAGDIQTVGDVEILNPDHVICTLDEGAEIRMEFTVDTGKGYVPAERNRA
EDAPIGLIPVDSLYSPVKKVSYKVENTREGQVLDYDKLTMTIETDGSISGEDAVAFAARILQDQLGLFVNFDEPQKEVAA
EAVTELAFNPALLKKVDELELSVRSANCLKNDNIVYIGDLIQKTEAEMLRTPNFGRKSLNEIKEVLAAMGLHLGMEVPDW
PPENIEDLAKRYEDQY

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=331, Percent_Identity=50.4531722054381, Blast_Score=313, Evalue=8e-87,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_RHILO (Q98N33)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_102144.1
- ProteinModelPortal:   Q98N33
- GeneID:   1224807
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr0325
- NMPDR:   fig|266835.1.peg.249
- HOGENOM:   HBG430844
- OMA:   FGTTLGN
- ProtClustDB:   PRK05182
- BRENDA:   2.7.7.6
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 37120; Mature: 37120

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQKNWQELIKPNKIEFSSKKKTLTTLVAEPLERGFGLTLGNALRRVLLSSLRGAAVTAV
CCCCCHHHHCCCCCEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEE
QIDGVLHEFSSIAGVREDVTDIVLNIKEIAIRMEGDGPKRMVVRKQGPGAVLAGDIQTVG
EHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEEEECCCCCEEECCCCCCC
DVEILNPDHVICTLDEGAEIRMEFTVDTGKGYVPAERNRAEDAPIGLIPVDSLYSPVKKV
CEEEECCCEEEEEECCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHH
SYKVENTREGQVLDYDKLTMTIETDGSISGEDAVAFAARILQDQLGLFVNFDEPQKEVAA
HEEECCCCCCCEEEECEEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEECCCHHHHHHH
EAVTELAFNPALLKKVDELELSVRSANCLKNDNIVYIGDLIQKTEAEMLRTPNFGRKSLN
HHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHCCCCCCHHHHH
EIKEVLAAMGLHLGMEVPDWPPENIEDLAKRYEDQY
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MIQKNWQELIKPNKIEFSSKKKTLTTLVAEPLERGFGLTLGNALRRVLLSSLRGAAVTAV
CCCCCHHHHCCCCCEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEE
QIDGVLHEFSSIAGVREDVTDIVLNIKEIAIRMEGDGPKRMVVRKQGPGAVLAGDIQTVG
EHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEEEECCCCCEEECCCCCCC
DVEILNPDHVICTLDEGAEIRMEFTVDTGKGYVPAERNRAEDAPIGLIPVDSLYSPVKKV
CEEEECCCEEEEEECCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHH
SYKVENTREGQVLDYDKLTMTIETDGSISGEDAVAFAARILQDQLGLFVNFDEPQKEVAA
HEEECCCCCCCEEEECEEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEECCCHHHHHHH
EAVTELAFNPALLKKVDELELSVRSANCLKNDNIVYIGDLIQKTEAEMLRTPNFGRKSLN
HHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHCCCCCCHHHHH
EIKEVLAAMGLHLGMEVPDWPPENIEDLAKRYEDQY
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968