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The map label for this gene is tuf

Identifier: 13470532

GI number: 13470532

Start: 185097

End: 186272

Strand: Direct

Name: tuf

Synonym: mlr0263

Alternate gene names: 13470532

Gene position: 185097-186272 (Clockwise)

Preceding gene: 13470528

Following gene: 13470533

Centisome position: 2.63

GC content: 62.93

Gene sequence:

>1176_bases
ATGGCAAAAGGTAAATTCGAGCGCACTAAGCCTCATGTGAACATCGGCACGATTGGTCACGTTGACCATGGCAAGACGTC
GCTGACGGCGGCGATCACGAAGTATTTTGGCGAGTACAAGCGCTACGACCAGATCGATGCGGCGCCGGAAGAGAAGGCAC
GCGGCATCACCATTTCGACGGCTCACGTCGAATACGAGACGGCCAACCGCCACTACGCCCACGTCGACTGCCCCGGCCAC
GCCGACTATGTGAAGAACATGATCACCGGCGCCGCGCAGATGGACGGCGCGATCCTGGTGGTGTCGGCCGCCGACGGCCC
GATGCCGCAGACCCGCGAGCACATCCTGCTTGCCCGTCAGGTCGGCGTGCCGTCGATCGTGGTGTTCCTGAACAAGGTCG
ACCAGGTCGACGACGCTGAGCTGCTCGAACTGGTCGAGCTCGAGGTTCGCGAGCTTCTGTCGAAGAACGAGTTCCCCGGC
GACGACATTCCGATCGTCAAGGGTTCGGCGCTGGCTGCTCTTGAGGATTCCAACAAGACGATCGGCGAGGACGCGATCCG
CGAGCTGATGGCTCAGGTCGACGCCTACATCCCGACCCCGGTTCGTCCGCTCGACAAGCCGTTCCTGATGCCGATCGAAG
ACGTGTTCTCGATCTCGGGCCGCGGCACGGTCGTGACCGGCCGCGTCGAGCGCGGCGTGGTCAAGGTCGGCGAGGAGCTG
GAGATCATCGGCATCCGTCCGACGACCAAGACGACCTGCACGGGCGTGGAAATGTTCCGCAAGCTGCTCGATCAGGGCCA
GGCTGGCGACAACATCGGCGCGCTGCTGCGTGGCGTTGATCGTGAAGGTGTCGAGCGCGGCCAGGTCCTGGCCAAGCCGG
GTACGGTGAAGCCGCACAAGAAGTTCGTGGCCGAAGCCTACATCCTGACCAAGGACGAAGGTGGCCGTCACACGCCGTTC
TTCACCAACTACCGTCCGCAGTTCTACTTCCGCACGACGGACGTGACCGGCATCGTGTCGCTGCCGGCTGGCACCGAGAT
GGTCATGCCTGGCGACAACATCACGGTCGATGTCGAGCTGATCGTACCGATCGCCATGGAAGAGAAGCTGCGCTTCGCCA
TCCGTGAAGGCGGGCGCACCGTCGGTGCCGGCATCGTCGTCACCATCAAGGAATAA

Upstream 100 bases:

>100_bases
ACGGCACCCGGCCAAAAGACGCTTGTGTTTTTTGGCCTTTGTCGCTAATCGCCCCGCATTCGACTGAACTGAAGGTCAAG
GCCGTCCAAGGAAAGAGACT

Downstream 100 bases:

>100_bases
TCGAGACCGGCATCAGCCGGATCGACAAGAAAAGGGCGGTCTTCGGGCCGCCCTTTTTCGTTTCCGTCGCCGCGTGAAAT
CGGAACTGCGCCCACCCTGT

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MAKGKFERTKPHVNIGTIGHVDHGKTSLTAAITKYFGEYKRYDQIDAAPEEKARGITISTAHVEYETANRHYAHVDCPGH
ADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPSIVVFLNKVDQVDDAELLELVELEVRELLSKNEFPG
DDIPIVKGSALAALEDSNKTIGEDAIRELMAQVDAYIPTPVRPLDKPFLMPIEDVFSISGRGTVVTGRVERGVVKVGEEL
EIIGIRPTTKTTCTGVEMFRKLLDQGQAGDNIGALLRGVDREGVERGQVLAKPGTVKPHKKFVAEAYILTKDEGGRHTPF
FTNYRPQFYFRTTDVTGIVSLPAGTEMVMPGDNITVDVELIVPIAMEEKLRFAIREGGRTVGAGIVVTIKE

Sequences:

>Translated_391_residues
MAKGKFERTKPHVNIGTIGHVDHGKTSLTAAITKYFGEYKRYDQIDAAPEEKARGITISTAHVEYETANRHYAHVDCPGH
ADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPSIVVFLNKVDQVDDAELLELVELEVRELLSKNEFPG
DDIPIVKGSALAALEDSNKTIGEDAIRELMAQVDAYIPTPVRPLDKPFLMPIEDVFSISGRGTVVTGRVERGVVKVGEEL
EIIGIRPTTKTTCTGVEMFRKLLDQGQAGDNIGALLRGVDREGVERGQVLAKPGTVKPHKKFVAEAYILTKDEGGRHTPF
FTNYRPQFYFRTTDVTGIVSLPAGTEMVMPGDNITVDVELIVPIAMEEKLRFAIREGGRTVGAGIVVTIKE
>Mature_390_residues
AKGKFERTKPHVNIGTIGHVDHGKTSLTAAITKYFGEYKRYDQIDAAPEEKARGITISTAHVEYETANRHYAHVDCPGHA
DYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPSIVVFLNKVDQVDDAELLELVELEVRELLSKNEFPGD
DIPIVKGSALAALEDSNKTIGEDAIRELMAQVDAYIPTPVRPLDKPFLMPIEDVFSISGRGTVVTGRVERGVVKVGEELE
IIGIRPTTKTTCTGVEMFRKLLDQGQAGDNIGALLRGVDREGVERGQVLAKPGTVKPHKKFVAEAYILTKDEGGRHTPFF
TNYRPQFYFRTTDVTGIVSLPAGTEMVMPGDNITVDVELIVPIAMEEKLRFAIREGGRTVGAGIVVTIKE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI34147630, Length=390, Percent_Identity=55.3846153846154, Blast_Score=466, Evalue=1e-131,
Organism=Homo sapiens, GI223555963, Length=435, Percent_Identity=28.5057471264368, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI5729864, Length=435, Percent_Identity=28.5057471264368, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI4503471, Length=442, Percent_Identity=29.185520361991, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI4503475, Length=444, Percent_Identity=27.7027027027027, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI54607086, Length=308, Percent_Identity=27.2727272727273, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI194018522, Length=329, Percent_Identity=29.483282674772, Blast_Score=113, Evalue=4e-25,
Organism=Homo sapiens, GI46094014, Length=326, Percent_Identity=29.7546012269939, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI194018520, Length=329, Percent_Identity=29.483282674772, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI194097354, Length=329, Percent_Identity=29.483282674772, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI4503507, Length=269, Percent_Identity=27.5092936802974, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI94966754, Length=136, Percent_Identity=33.8235294117647, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1790412, Length=394, Percent_Identity=74.8730964467005, Blast_Score=577, Evalue=1e-166,
Organism=Escherichia coli, GI1789737, Length=394, Percent_Identity=74.8730964467005, Blast_Score=577, Evalue=1e-166,
Organism=Escherichia coli, GI2367247, Length=381, Percent_Identity=31.2335958005249, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI48994988, Length=251, Percent_Identity=33.4661354581673, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1789108, Length=255, Percent_Identity=27.4509803921569, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1789738, Length=169, Percent_Identity=30.7692307692308, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=400, Percent_Identity=51.25, Blast_Score=394, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI25141371, Length=391, Percent_Identity=42.4552429667519, Blast_Score=313, Evalue=1e-85,
Organism=Caenorhabditis elegans, GI17552884, Length=452, Percent_Identity=29.4247787610619, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17569207, Length=452, Percent_Identity=29.4247787610619, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI32566303, Length=432, Percent_Identity=27.0833333333333, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI115532067, Length=439, Percent_Identity=27.7904328018223, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI115532065, Length=436, Percent_Identity=27.7522935779816, Blast_Score=124, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI32566629, Length=354, Percent_Identity=28.2485875706215, Blast_Score=114, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI17506081, Length=261, Percent_Identity=29.8850574712644, Blast_Score=111, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI32566301, Length=166, Percent_Identity=34.3373493975904, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17509919, Length=277, Percent_Identity=25.9927797833935, Blast_Score=81, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17557151, Length=234, Percent_Identity=26.4957264957265, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324761, Length=396, Percent_Identity=65.6565656565657, Blast_Score=518, Evalue=1e-148,
Organism=Saccharomyces cerevisiae, GI6325337, Length=443, Percent_Identity=29.1196388261851, Blast_Score=152, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6319594, Length=443, Percent_Identity=29.1196388261851, Blast_Score=152, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6322937, Length=352, Percent_Identity=25.5681818181818, Blast_Score=90, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6320377, Length=357, Percent_Identity=26.3305322128852, Blast_Score=87, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6320863, Length=269, Percent_Identity=27.5092936802974, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323098, Length=179, Percent_Identity=30.1675977653631, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322359, Length=131, Percent_Identity=31.2977099236641, Blast_Score=66, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6323320, Length=239, Percent_Identity=29.2887029288703, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI281363316, Length=391, Percent_Identity=59.079283887468, Blast_Score=483, Evalue=1e-137,
Organism=Drosophila melanogaster, GI17864358, Length=391, Percent_Identity=59.079283887468, Blast_Score=483, Evalue=1e-137,
Organism=Drosophila melanogaster, GI19921738, Length=384, Percent_Identity=49.7395833333333, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24652838, Length=449, Percent_Identity=28.9532293986637, Blast_Score=151, Evalue=8e-37,
Organism=Drosophila melanogaster, GI17137572, Length=449, Percent_Identity=28.9532293986637, Blast_Score=151, Evalue=8e-37,
Organism=Drosophila melanogaster, GI45553807, Length=449, Percent_Identity=28.2850779510022, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI45553816, Length=449, Percent_Identity=28.2850779510022, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI24651721, Length=449, Percent_Identity=28.2850779510022, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI17864154, Length=449, Percent_Identity=28.2850779510022, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI19922690, Length=300, Percent_Identity=31, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI45550900, Length=324, Percent_Identity=29.0123456790123, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI17137380, Length=342, Percent_Identity=30.9941520467836, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24647046, Length=269, Percent_Identity=26.3940520446097, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24647048, Length=269, Percent_Identity=26.3940520446097, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI28572034, Length=176, Percent_Identity=37.5, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): EFTU_RHILO (Q981F7)

Other databases:

- EMBL:   BA000012
- EMBL:   BA000012
- RefSeq:   NP_102100.1
- RefSeq:   NP_102118.1
- ProteinModelPortal:   Q981F7
- SMR:   Q981F7
- GeneID:   1224763
- GeneID:   1224781
- GenomeReviews:   BA000012_GR
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr0263
- KEGG:   mlo:mlr0288
- HOGENOM:   HBG307581
- OMA:   FEGQVYV
- GO:   GO:0005737
- HAMAP:   MF_00118_B
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000
- PANTHER:   PTHR23115:SF31
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00485
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: 3.6.5.3

Molecular weight: Translated: 42714; Mature: 42582

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKGKFERTKPHVNIGTIGHVDHGKTSLTAAITKYFGEYKRYDQIDAAPEEKARGITIST
CCCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCEEEEE
AHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQ
EEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHEEEHHH
VGVPSIVVFLNKVDQVDDAELLELVELEVRELLSKNEFPGDDIPIVKGSALAALEDSNKT
CCCCEEEEEEHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCEEEEEECCCCC
IGEDAIRELMAQVDAYIPTPVRPLDKPFLMPIEDVFSISGRGTVVTGRVERGVVKVGEEL
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCCEEEECHHHCCHHCCCCCE
EIIGIRPTTKTTCTGVEMFRKLLDQGQAGDNIGALLRGVDREGVERGQVLAKPGTVKPHK
EEEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCEEECCCCCCHHH
KFVAEAYILTKDEGGRHTPFFTNYRPQFYFRTTDVTGIVSLPAGTEMVMPGDNITVDVEL
HHHEEEEEEEECCCCCCCCEEECCCCEEEEEECCCEEEEECCCCCEEEECCCCEEEEEEE
IVPIAMEEKLRFAIREGGRTVGAGIVVTIKE
EEEEHHHHHHHHHHHHCCEEECCEEEEEEEC
>Mature Secondary Structure 
AKGKFERTKPHVNIGTIGHVDHGKTSLTAAITKYFGEYKRYDQIDAAPEEKARGITIST
CCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCEEEEE
AHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQ
EEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHEEEHHH
VGVPSIVVFLNKVDQVDDAELLELVELEVRELLSKNEFPGDDIPIVKGSALAALEDSNKT
CCCCEEEEEEHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCEEEEEECCCCC
IGEDAIRELMAQVDAYIPTPVRPLDKPFLMPIEDVFSISGRGTVVTGRVERGVVKVGEEL
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCCEEEECHHHCCHHCCCCCE
EIIGIRPTTKTTCTGVEMFRKLLDQGQAGDNIGALLRGVDREGVERGQVLAKPGTVKPHK
EEEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCEEECCCCCCHHH
KFVAEAYILTKDEGGRHTPFFTNYRPQFYFRTTDVTGIVSLPAGTEMVMPGDNITVDVEL
HHHEEEEEEEECCCCCCCCEEECCCCEEEEEECCCEEEEECCCCCEEEECCCCEEEEEEE
IVPIAMEEKLRFAIREGGRTVGAGIVVTIKE
EEEEHHHHHHHHHHHHCCEEECCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968