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The map label for this gene is ppnK [H]

Identifier: 13470500

GI number: 13470500

Start: 158053

End: 158907

Strand: Reverse

Name: ppnK [H]

Synonym: mll0225

Alternate gene names: 13470500

Gene position: 158907-158053 (Counterclockwise)

Preceding gene: 13470505

Following gene: 13470499

Centisome position: 2.26

GC content: 65.73

Gene sequence:

>855_bases
TTGCAGAGCTGCCCACCCCGTGCTACCCCGGCGCGGGCGCGGCCAGACCTGCCGCCGCCAATCGGTCGCGATCGGGAAGC
CATGAGCACAGCCGCCAACAGCATCGCCTTCGTCTCGTCCGACACAGCCGACGCCAAGGCGGCGCTGGAAAGTCTGTCGG
CGCGCTATGGCCAGTGTTCGGTCGCCGAGGCCGTGGTTGTGGTGGCCCTGGGCGGTGACGGCTTCCTGCTGCAGACCTTG
CGTGACACGATGGGAACGGGAAAGAAGGTCTACGGCATGAACCGTGGCACCATCGGGTTCCTGATGAATGAGTACCGTTC
GGGGGGGCTCACCGAGCGCATCGCGGCCGCGGTCGCCGAAACCATCCGGCCGCTGGAGATGCTGGCCGTCACCTCGGAAG
GCGAAACCGTCTCGGCGCTGGCGATCAACGAGGTTGCGCTGTGGCGCCAATCCTACCAGACGGCTAAGATCCGCATCAGC
GTCGATGACCAGATACGGCTCGAGGAACTCAGCTGCGATGGTGTCATGATCGCGACGCCGGCCGGATCCACCGCCTACAA
TCTTTCCGCGCATGGTCCGATCCTGCCGCTCGACGCGCCGCTTCTGGCACTGACACCCGTCAGTCCGTTTCGCCCGCGCC
GCTGGCGTGGCGCGTTGCTTTCCAACAAGGCGACTGTGCGCTTCGACATCCTGGAGCCGGAAAAGCGGCCGGTGAACGCC
GCCGCCGACCATACGGAGGTCAAGGCGGTGACCTCGGTAACGGTGCGGGAGTCGCCGACAGCGACGGCAACCTTGCTGTT
CGACCCCAATCACTCCTGGAACGAGCGCATCCTGGCCGAGCAGTTCCGTTACTGA

Upstream 100 bases:

>100_bases
CCTTAACCATCACATTCGCGCGTGCCCTGTCATGAAGCGCGATCCGCAGGTTCGCACGATGCTGTCGCCGCAGCCTTGCA
CGCCGCCGCAACGGCTCTCT

Downstream 100 bases:

>100_bases
GCCGATAGTGACAAACGGCCGGATTAAGCATCGCGATTAAGGCTACAACTTCACAATCGTGCCAATCCCGCCCGTTTCTG
TTGACAAATCGCGGGCTTGC

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase [H]

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MQSCPPRATPARARPDLPPPIGRDREAMSTAANSIAFVSSDTADAKAALESLSARYGQCSVAEAVVVVALGGDGFLLQTL
RDTMGTGKKVYGMNRGTIGFLMNEYRSGGLTERIAAAVAETIRPLEMLAVTSEGETVSALAINEVALWRQSYQTAKIRIS
VDDQIRLEELSCDGVMIATPAGSTAYNLSAHGPILPLDAPLLALTPVSPFRPRRWRGALLSNKATVRFDILEPEKRPVNA
AADHTEVKAVTSVTVRESPTATATLLFDPNHSWNERILAEQFRY

Sequences:

>Translated_284_residues
MQSCPPRATPARARPDLPPPIGRDREAMSTAANSIAFVSSDTADAKAALESLSARYGQCSVAEAVVVVALGGDGFLLQTL
RDTMGTGKKVYGMNRGTIGFLMNEYRSGGLTERIAAAVAETIRPLEMLAVTSEGETVSALAINEVALWRQSYQTAKIRIS
VDDQIRLEELSCDGVMIATPAGSTAYNLSAHGPILPLDAPLLALTPVSPFRPRRWRGALLSNKATVRFDILEPEKRPVNA
AADHTEVKAVTSVTVRESPTATATLLFDPNHSWNERILAEQFRY
>Mature_284_residues
MQSCPPRATPARARPDLPPPIGRDREAMSTAANSIAFVSSDTADAKAALESLSARYGQCSVAEAVVVVALGGDGFLLQTL
RDTMGTGKKVYGMNRGTIGFLMNEYRSGGLTERIAAAVAETIRPLEMLAVTSEGETVSALAINEVALWRQSYQTAKIRIS
VDDQIRLEELSCDGVMIATPAGSTAYNLSAHGPILPLDAPLLALTPVSPFRPRRWRGALLSNKATVRFDILEPEKRPVNA
AADHTEVKAVTSVTVRESPTATATLLFDPNHSWNERILAEQFRY

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus [H]

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family [H]

Homologues:

Organism=Escherichia coli, GI1788968, Length=172, Percent_Identity=30.2325581395349, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504 [H]

Pfam domain/function: PF01513 NAD_kinase [H]

EC number: =2.7.1.23 [H]

Molecular weight: Translated: 30608; Mature: 30608

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSCPPRATPARARPDLPPPIGRDREAMSTAANSIAFVSSDTADAKAALESLSARYGQCS
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCEEEEECCCHHHHHHHHHHHHHCCCCC
VAEAVVVVALGGDGFLLQTLRDTMGTGKKVYGMNRGTIGFLMNEYRSGGLTERIAAAVAE
CCCEEEEEEECCCCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHH
TIRPLEMLAVTSEGETVSALAINEVALWRQSYQTAKIRISVDDQIRLEELSCDGVMIATP
HHCCEEEEEEECCCCEEEEEEHHHHHHHHHCCCEEEEEEEECCCEEEEEECCCCEEEECC
AGSTAYNLSAHGPILPLDAPLLALTPVSPFRPRRWRGALLSNKATVRFDILEPEKRPVNA
CCCCEEEECCCCCEEECCCCEEEECCCCCCCCCCCCCHHHCCCCEEEEEEECCCCCCCCC
AADHTEVKAVTSVTVRESPTATATLLFDPNHSWNERILAEQFRY
CCCCCCEEEEEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MQSCPPRATPARARPDLPPPIGRDREAMSTAANSIAFVSSDTADAKAALESLSARYGQCS
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCEEEEECCCHHHHHHHHHHHHHCCCCC
VAEAVVVVALGGDGFLLQTLRDTMGTGKKVYGMNRGTIGFLMNEYRSGGLTERIAAAVAE
CCCEEEEEEECCCCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHH
TIRPLEMLAVTSEGETVSALAINEVALWRQSYQTAKIRISVDDQIRLEELSCDGVMIATP
HHCCEEEEEEECCCCEEEEEEHHHHHHHHHCCCEEEEEEEECCCEEEEEECCCCEEEECC
AGSTAYNLSAHGPILPLDAPLLALTPVSPFRPRRWRGALLSNKATVRFDILEPEKRPVNA
CCCCEEEECCCCCEEECCCCEEEECCCCCCCCCCCCCHHHCCCCEEEEEEECCCCCCCCC
AADHTEVKAVTSVTVRESPTATATLLFDPNHSWNERILAEQFRY
CCCCCCEEEEEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]