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The map label for this gene is prfB [H]

Identifier: 13470493

GI number: 13470493

Start: 152441

End: 153463

Strand: Direct

Name: prfB [H]

Synonym: mlr0216

Alternate gene names: 13470493

Gene position: 152441-153463 (Clockwise)

Preceding gene: 13470492

Following gene: 13470494

Centisome position: 2.17

GC content: 64.52

Gene sequence:

>1023_bases
GTGCGGGCCGAGGACGCCAGCCTCTGGAACGAACCGCTGGAAGCGCAGAAGCTGATGCGCGAGCGTCAGGGCCTCGAGGA
AGGCATCGCGGCGGTCAAGGGCCTGACCCAGGCGCTGGAAGACAATATCGGCCTGATCGAGCTCGGCGAGGAAGAGGGCG
ACGAGGGCGTCATCACCGAGGCCGAAGCGGCGATCCGCTCGATGCAGGGCGAGGCCAAGGCCCGCCAGGTCGAAACGCTG
CTGTCGGGTGAAGCCGACGCCAACGACACCTATCTCGAAATCCATGCCGGCGCCGGCGGCACCGAAAGCCAGGACTGGGC
CTCGATGCTTTTGCGCATGTACACGCGCTGGGCCGAACGCCGCCGCTTCAAAGTCGAAGTGCTGGAAGTGCATGACGGCG
AAGAGGCCGGCATCAAGTCCGCGACGCTGATGATCAAGGGCCACAATGCCTATGGCTGGCTGAAGACTGAATCCGGCGTC
CATCGCCTGGTCCGCATTTCGCCCTATGACAGCAATGCGCGCCGGCACACTTCCTTCGCCAGCGTCTGGGTCTATCCGGT
CATCGACGACACGATCGAGATCGATGTTTCAGAATCGGACGTGCGCATCGACACCTATCGCTCGTCGGGCTCTGGCGGCC
AGCACGTCAACACCACCGATTCGGCTGTGCGCATCACCCATATCGCCACCGGCATCGCGGTCGCCTGCCAGGCCGAGCGT
TCGCAGCACAAGAACCGGGCGAAGGCCTGGGAGATGCTGCGCTCGCGCCTTTACGAGGAAGAGCTGAAGAAGCGTGAGGC
CATCGCCAACGCCACCGAAGCCTCGAAGAGCGACATCGGCTGGGGCCACCAGATCCGCTCCTACGTGCTGCAGCCGTACC
AGTTGGTGAAGGATCTGCGCACCGGCGTCGAAAGCACCAGCCCGTCGAGCGTGCTCGATGGCGACCTCGACGACTTCATG
GAAGCCTCGCTGTCGCAACGCATCGAAGGCGGCGCAGGCGAGGCCGTGGCGGATCTGGACTAG

Upstream 100 bases:

>100_bases
GAAACGCAGAATATTGTCGACGAGATCAGGCAGGCGATAACCCTGCTGAGGAGGCATCTTTGACTGGGATCAGGCCATAA
AGCGGCTTGAATACCTGAAT

Downstream 100 bases:

>100_bases
CACCGTCCGCCGCCATTGCGCCCGCTCGCATTTTCTGCGGACCAGGTGGGCCGCCTTGGGCGGCCCGCGGCTCCACGCAG
CAATTTCTTGCGCCGACTCA

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MRAEDASLWNEPLEAQKLMRERQGLEEGIAAVKGLTQALEDNIGLIELGEEEGDEGVITEAEAAIRSMQGEAKARQVETL
LSGEADANDTYLEIHAGAGGTESQDWASMLLRMYTRWAERRRFKVEVLEVHDGEEAGIKSATLMIKGHNAYGWLKTESGV
HRLVRISPYDSNARRHTSFASVWVYPVIDDTIEIDVSESDVRIDTYRSSGSGGQHVNTTDSAVRITHIATGIAVACQAER
SQHKNRAKAWEMLRSRLYEEELKKREAIANATEASKSDIGWGHQIRSYVLQPYQLVKDLRTGVESTSPSSVLDGDLDDFM
EASLSQRIEGGAGEAVADLD

Sequences:

>Translated_340_residues
MRAEDASLWNEPLEAQKLMRERQGLEEGIAAVKGLTQALEDNIGLIELGEEEGDEGVITEAEAAIRSMQGEAKARQVETL
LSGEADANDTYLEIHAGAGGTESQDWASMLLRMYTRWAERRRFKVEVLEVHDGEEAGIKSATLMIKGHNAYGWLKTESGV
HRLVRISPYDSNARRHTSFASVWVYPVIDDTIEIDVSESDVRIDTYRSSGSGGQHVNTTDSAVRITHIATGIAVACQAER
SQHKNRAKAWEMLRSRLYEEELKKREAIANATEASKSDIGWGHQIRSYVLQPYQLVKDLRTGVESTSPSSVLDGDLDDFM
EASLSQRIEGGAGEAVADLD
>Mature_340_residues
MRAEDASLWNEPLEAQKLMRERQGLEEGIAAVKGLTQALEDNIGLIELGEEEGDEGVITEAEAAIRSMQGEAKARQVETL
LSGEADANDTYLEIHAGAGGTESQDWASMLLRMYTRWAERRRFKVEVLEVHDGEEAGIKSATLMIKGHNAYGWLKTESGV
HRLVRISPYDSNARRHTSFASVWVYPVIDDTIEIDVSESDVRIDTYRSSGSGGQHVNTTDSAVRITHIATGIAVACQAER
SQHKNRAKAWEMLRSRLYEEELKKREAIANATEASKSDIGWGHQIRSYVLQPYQLVKDLRTGVESTSPSSVLDGDLDDFM
EASLSQRIEGGAGEAVADLD

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=247, Percent_Identity=39.6761133603239, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI34577120, Length=341, Percent_Identity=32.8445747800587, Blast_Score=138, Evalue=7e-33,
Organism=Homo sapiens, GI166795305, Length=149, Percent_Identity=43.6241610738255, Blast_Score=123, Evalue=3e-28,
Organism=Escherichia coli, GI2367172, Length=321, Percent_Identity=55.1401869158878, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI1787462, Length=244, Percent_Identity=43.0327868852459, Blast_Score=193, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI17542784, Length=245, Percent_Identity=33.8775510204082, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6321295, Length=260, Percent_Identity=40, Blast_Score=153, Evalue=3e-38,
Organism=Drosophila melanogaster, GI19921226, Length=196, Percent_Identity=43.8775510204082, Blast_Score=159, Evalue=3e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 37578; Mature: 37578

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAEDASLWNEPLEAQKLMRERQGLEEGIAAVKGLTQALEDNIGLIELGEEEGDEGVITE
CCCCCCHHHCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEH
AEAAIRSMQGEAKARQVETLLSGEADANDTYLEIHAGAGGTESQDWASMLLRMYTRWAER
HHHHHHHHCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
RRFKVEVLEVHDGEEAGIKSATLMIKGHNAYGWLKTESGVHRLVRISPYDSNARRHTSFA
CCEEEEEEEECCCCCCCCCEEEEEEEECCCCEEEECCCCCEEEEEECCCCCCCHHHCCEE
SVWVYPVIDDTIEIDVSESDVRIDTYRSSGSGGQHVNTTDSAVRITHIATGIAVACQAER
EEEEEEEECCEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHH
SQHKNRAKAWEMLRSRLYEEELKKREAIANATEASKSDIGWGHQIRSYVLQPYQLVKDLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
TGVESTSPSSVLDGDLDDFMEASLSQRIEGGAGEAVADLD
HCCCCCCCCHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MRAEDASLWNEPLEAQKLMRERQGLEEGIAAVKGLTQALEDNIGLIELGEEEGDEGVITE
CCCCCCHHHCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEH
AEAAIRSMQGEAKARQVETLLSGEADANDTYLEIHAGAGGTESQDWASMLLRMYTRWAER
HHHHHHHHCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
RRFKVEVLEVHDGEEAGIKSATLMIKGHNAYGWLKTESGVHRLVRISPYDSNARRHTSFA
CCEEEEEEEECCCCCCCCCEEEEEEEECCCCEEEECCCCCEEEEEECCCCCCCHHHCCEE
SVWVYPVIDDTIEIDVSESDVRIDTYRSSGSGGQHVNTTDSAVRITHIATGIAVACQAER
EEEEEEEECCEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHH
SQHKNRAKAWEMLRSRLYEEELKKREAIANATEASKSDIGWGHQIRSYVLQPYQLVKDLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
TGVESTSPSSVLDGDLDDFMEASLSQRIEGGAGEAVADLD
HCCCCCCCCHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA