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The map label for this gene is gatB

Identifier: 13470473

GI number: 13470473

Start: 130538

End: 132037

Strand: Direct

Name: gatB

Synonym: mlr0190

Alternate gene names: 13470473

Gene position: 130538-132037 (Clockwise)

Preceding gene: 13470471

Following gene: 13470474

Centisome position: 1.86

GC content: 62.2

Gene sequence:

>1500_bases
ATGACCATCATCGATACCCGCACGCCCGACGCAAAACGCCTGATCCCCGGCGCCACCGGTGACTGGGAAATCATCATCGG
TCTCGAAGTGCACGCGCAGGTCATCTCGGAAGCAAAACTGTTTTCCGGCGCCTCGACTGCCTTCGGCGCGGCCCCCAACG
CCAATGTCAGCCTGGTCGATGCGGCAATGCCGGGCATGCTGCCTGTCATCAACGAGGAATGCGTCAAGCAGGCGATCCGC
ACCGGGCTCGGGCTGAAGGCGCAGATCAACCACAAGTCGGTTTTCGACCGGAAGAACTATTTTTATCCCGATCTGCCGCA
GGGCTATCAGATCTCGCAGTTCAAGCAGCCGATCGTCGGCGAGGGCACCGTCATCGTTTCGGTCGGCCCCGACCGCCAGG
GCGAGTTCGAGGACATCGAGGTCGGCATCGAGCGGCTGCATCTGGAACAGGATGCCGGCAAGTCGATGCACGACCAGCAT
CCGACCATGTCCTATGTCGACCTCAACCGCTCCGGCGTGGCGCTGATGGAAATCGTCTCCAAGCCCGACCTGCGTTCCGG
CGACGAGGCCAAGGCTTATGTCACCAAGCTGCGCACCATCATGCGCTATCTCGGCACCTGCGACGGCAACATGGATGAAG
GCTCGCTGCGCGCCGACGTCAACGTCTCGGTGCGCCGGCCCGGTGGCGAGTTCGGCACCCGCTGCGAGATCAAGAACATG
AACTCGATCCGCTTCATCGGCCAGGCCATCGACTATGAGGCGCGGCGCCAGATCGCCATTCTCGAGGACGGCGGCAAGAT
CGATCAGGAAACGCGGCTGTACGATGCCGTCAAGGGCGAGACGCGCTCCATGCGCTCGAAGGAAGAAGCGCACGACTACC
GCTATTTCCCTGATCCCGATCTTCTGCCGCTGGAATTCGATCAGGCCTATGTCGATGCTTTGGCCAAAGAGCTGCCGGAA
CTGCCCGACGACAAGAAGGCGCGGCTGATCACCTCGCTGGGTCTCTCGACCTACGACGCTTCGATCCTGGTGTCGGAAAA
GTCGGTCGCCGACTATTTCGAGAAGGTGGCCGCCGGCCGCGACGGCAAGCTCGCCGCCAACTGGGTCATCAACGATCTGC
TCGGCCAGTTGAACAAGGTCGGCAAGGACATTGAAAATGCTCCCGTTTCGCCCGACCAGCTCGGCGCGATCATCGACCTG
ATCAAGGACGGCACCATCTCCGGCAAGATCGCCAAGGACCTGTTCGAGATCGTCTGGAGCGAAGGCGGCGATCCACGTGC
GCTGGTCGAAAGCCGTGGCATGAAGCAGGTCACCGACACCGGCGCCATCGAGAAGGCGGTCGACGAGGTGATCGCGGCCA
ATCCCGACAAGGTGGAACAGGCGCGCGCCAAGCCGACCATGGCCGGCTGGTTCGTTGGCCAGGTGATGAAAGCGACGGGC
GGCAAGGCCAATCCGCAGGCGGTCAACGACCTCGTCAAGGCCAAGCTCGGCATCGAGTAA

Upstream 100 bases:

>100_bases
GCTTGTCTCTTTTGGCGGTTTGCACTAGCGAAGCGGGTACGATTTGTGCCCCTGAATGTGTAGTGACATTCAGCATCCAT
CCCGAATTTGACGGCAGGCC

Downstream 100 bases:

>100_bases
GGTCATGTTCGTCCGCACCGCAGGCGAACGCGATCTCGTTGCCATCCGCGCGCTGCTGGTCGAAACTTGGCATGCCACCT
ATGACGCGATCTATGGCGCT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 499; Mature: 498

Protein sequence:

>499_residues
MTIIDTRTPDAKRLIPGATGDWEIIIGLEVHAQVISEAKLFSGASTAFGAAPNANVSLVDAAMPGMLPVINEECVKQAIR
TGLGLKAQINHKSVFDRKNYFYPDLPQGYQISQFKQPIVGEGTVIVSVGPDRQGEFEDIEVGIERLHLEQDAGKSMHDQH
PTMSYVDLNRSGVALMEIVSKPDLRSGDEAKAYVTKLRTIMRYLGTCDGNMDEGSLRADVNVSVRRPGGEFGTRCEIKNM
NSIRFIGQAIDYEARRQIAILEDGGKIDQETRLYDAVKGETRSMRSKEEAHDYRYFPDPDLLPLEFDQAYVDALAKELPE
LPDDKKARLITSLGLSTYDASILVSEKSVADYFEKVAAGRDGKLAANWVINDLLGQLNKVGKDIENAPVSPDQLGAIIDL
IKDGTISGKIAKDLFEIVWSEGGDPRALVESRGMKQVTDTGAIEKAVDEVIAANPDKVEQARAKPTMAGWFVGQVMKATG
GKANPQAVNDLVKAKLGIE

Sequences:

>Translated_499_residues
MTIIDTRTPDAKRLIPGATGDWEIIIGLEVHAQVISEAKLFSGASTAFGAAPNANVSLVDAAMPGMLPVINEECVKQAIR
TGLGLKAQINHKSVFDRKNYFYPDLPQGYQISQFKQPIVGEGTVIVSVGPDRQGEFEDIEVGIERLHLEQDAGKSMHDQH
PTMSYVDLNRSGVALMEIVSKPDLRSGDEAKAYVTKLRTIMRYLGTCDGNMDEGSLRADVNVSVRRPGGEFGTRCEIKNM
NSIRFIGQAIDYEARRQIAILEDGGKIDQETRLYDAVKGETRSMRSKEEAHDYRYFPDPDLLPLEFDQAYVDALAKELPE
LPDDKKARLITSLGLSTYDASILVSEKSVADYFEKVAAGRDGKLAANWVINDLLGQLNKVGKDIENAPVSPDQLGAIIDL
IKDGTISGKIAKDLFEIVWSEGGDPRALVESRGMKQVTDTGAIEKAVDEVIAANPDKVEQARAKPTMAGWFVGQVMKATG
GKANPQAVNDLVKAKLGIE
>Mature_498_residues
TIIDTRTPDAKRLIPGATGDWEIIIGLEVHAQVISEAKLFSGASTAFGAAPNANVSLVDAAMPGMLPVINEECVKQAIRT
GLGLKAQINHKSVFDRKNYFYPDLPQGYQISQFKQPIVGEGTVIVSVGPDRQGEFEDIEVGIERLHLEQDAGKSMHDQHP
TMSYVDLNRSGVALMEIVSKPDLRSGDEAKAYVTKLRTIMRYLGTCDGNMDEGSLRADVNVSVRRPGGEFGTRCEIKNMN
SIRFIGQAIDYEARRQIAILEDGGKIDQETRLYDAVKGETRSMRSKEEAHDYRYFPDPDLLPLEFDQAYVDALAKELPEL
PDDKKARLITSLGLSTYDASILVSEKSVADYFEKVAAGRDGKLAANWVINDLLGQLNKVGKDIENAPVSPDQLGAIIDLI
KDGTISGKIAKDLFEIVWSEGGDPRALVESRGMKQVTDTGAIEKAVDEVIAANPDKVEQARAKPTMAGWFVGQVMKATGG
KANPQAVNDLVKAKLGIE

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=495, Percent_Identity=40.2020202020202, Blast_Score=375, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI115534094, Length=298, Percent_Identity=42.2818791946309, Blast_Score=223, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6319391, Length=529, Percent_Identity=30.812854442344, Blast_Score=225, Evalue=1e-59,
Organism=Drosophila melanogaster, GI45550808, Length=488, Percent_Identity=39.344262295082, Blast_Score=347, Evalue=1e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_RHILO (Q98ND3)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_102042.1
- ProteinModelPortal:   Q98ND3
- SMR:   Q98ND3
- GeneID:   1224705
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr0190
- NMPDR:   fig|266835.1.peg.147
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- ProtClustDB:   PRK05477
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR023168
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- Gene3D:   G3DSA:1.10.10.410
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 54493; Mature: 54361

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIIDTRTPDAKRLIPGATGDWEIIIGLEVHAQVISEAKLFSGASTAFGAAPNANVSLVD
CCEECCCCCCHHHCCCCCCCCEEEEEEEHHHHHHHHHHHHHCCCCHHCCCCCCCCEEEEE
AAMPGMLPVINEECVKQAIRTGLGLKAQINHKSVFDRKNYFYPDLPQGYQISQFKQPIVG
CCCCCCCHHHHHHHHHHHHHHCCCCEEEECCHHHHCCCCCCCCCCCCCCCHHHHCCCCCC
EGTVIVSVGPDRQGEFEDIEVGIERLHLEQDAGKSMHDQHPTMSYVDLNRSGVALMEIVS
CCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCHHHHHHHC
KPDLRSGDEAKAYVTKLRTIMRYLGTCDGNMDEGSLRADVNVSVRRPGGEFGTRCEIKNM
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCCCEEEECCC
NSIRFIGQAIDYEARRQIAILEDGGKIDQETRLYDAVKGETRSMRSKEEAHDYRYFPDPD
CHHHHHHHHHCHHHHCEEEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCC
LLPLEFDQAYVDALAKELPELPDDKKARLITSLGLSTYDASILVSEKSVADYFEKVAAGR
CCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHCCC
DGKLAANWVINDLLGQLNKVGKDIENAPVSPDQLGAIIDLIKDGTISGKIAKDLFEIVWS
CCCEEHHHHHHHHHHHHHHHHCHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHC
EGGDPRALVESRGMKQVTDTGAIEKAVDEVIAANPDKVEQARAKPTMAGWFVGQVMKATG
CCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHCC
GKANPQAVNDLVKAKLGIE
CCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TIIDTRTPDAKRLIPGATGDWEIIIGLEVHAQVISEAKLFSGASTAFGAAPNANVSLVD
CEECCCCCCHHHCCCCCCCCEEEEEEEHHHHHHHHHHHHHCCCCHHCCCCCCCCEEEEE
AAMPGMLPVINEECVKQAIRTGLGLKAQINHKSVFDRKNYFYPDLPQGYQISQFKQPIVG
CCCCCCCHHHHHHHHHHHHHHCCCCEEEECCHHHHCCCCCCCCCCCCCCCHHHHCCCCCC
EGTVIVSVGPDRQGEFEDIEVGIERLHLEQDAGKSMHDQHPTMSYVDLNRSGVALMEIVS
CCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCHHHHHHHC
KPDLRSGDEAKAYVTKLRTIMRYLGTCDGNMDEGSLRADVNVSVRRPGGEFGTRCEIKNM
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCCCEEEECCC
NSIRFIGQAIDYEARRQIAILEDGGKIDQETRLYDAVKGETRSMRSKEEAHDYRYFPDPD
CHHHHHHHHHCHHHHCEEEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCC
LLPLEFDQAYVDALAKELPELPDDKKARLITSLGLSTYDASILVSEKSVADYFEKVAAGR
CCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHCCC
DGKLAANWVINDLLGQLNKVGKDIENAPVSPDQLGAIIDLIKDGTISGKIAKDLFEIVWS
CCCEEHHHHHHHHHHHHHHHHCHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHC
EGGDPRALVESRGMKQVTDTGAIEKAVDEVIAANPDKVEQARAKPTMAGWFVGQVMKATG
CCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHCC
GKANPQAVNDLVKAKLGIE
CCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968