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The map label for this gene is ilvA [H]

Identifier: 13470452

GI number: 13470452

Start: 115271

End: 116572

Strand: Direct

Name: ilvA [H]

Synonym: mlr0162

Alternate gene names: 13470452

Gene position: 115271-116572 (Clockwise)

Preceding gene: 13470450

Following gene: 13470453

Centisome position: 1.64

GC content: 62.37

Gene sequence:

>1302_bases
ATGACAGGGGGGCCGCGCAGACATGCGGCACAAGGTGGCTTTCCGATGAACAGTTTTTCCTCCCGCGTCGCCACCGCTGC
GGCGGCGATCCGCCAGATCTTTCCGGAAACGCCGCTGCAGGAAAACGACTATCTGTCGAAGAAGACCGGCGCGCGGGTGC
TGCTGAAGCGGGAGGACCTGTCGCCGGTGCGCTCCTACAAGATCCGTGGCGCCTTCAACTTCTTCCGCAAGGCGCTCGGC
GAGGGCAACCAGGCAGAGCTGTTCGTCTGTGCGTCCGCCGGCAACCATGCCCAGGGTTTTGCCTTCGTCTGCCGCCATTT
CGGCAAAAAGGGCGTGGTGTTCATGCCGGTGACAACGCCGCAGCAGAAGATCGACAAGACCAGGCTGTTCGGCGGTGATT
TCGTCGAGATCAGGCTGGTCGGCGACTTTTTCGACGACTGCTATCGCGCCGCCTTCGAATTCACCGAAAGGTCGGGTGCC
CACATGGTGCCGCCCTTCGACCACAAGGACATTATCGAGGGCCAGGCGACGGTCGCCTATGAGATCGCGGCCCAGATGGC
GGGTGCGCGCATGCCTGACATCGTCATGCTGCCGGTCGGCGGTGGCGGGCTCGCCGCCGGCGTCACGCACTATTTCGCCG
ATCAGGGCCGCGAGGCGCGTTTTGTCTTCTGCGAGCCGGCCGGCGCGCCGAGCCTGCACGACAGCCTCGCGGCCGGAAAA
CGGCTGAAGCTCGCCAAGGTCGACAACTTCGTCGACGGCGCGGCGGTGGCCGAAATCGGCCGCGAACCCTTGCGGTTTCT
CAAGGAATTCGCTGCTGACACCGTGCGGCTGATCCCGGAAAACCGGCTCTGCGCCACCATGATCGAGATGCTCAATGTCG
AAGGCGTCGTGCTCGAGCCGGCCGGCGCATTGGCCATCGACGCATTGAAGGATTTTTCCAGGAAGGAGATCCGTGGCAAG
ACCATTGTCGCCGTGGTCTCAGGCGGCAATTTCGATTTCGAGCGGCTGCCGGATGTCAAGGAACGGGCGCTGCGCTTCGA
AGGCCTGAAGAAATATTTCATCATCCGCTTCCCGCAGCGGCCGGGCGCGCTGCGCGACTTCCTCGAAATGCTGGGACCCG
ACGACGACATTGCCCGCTTCGAGTATCTGAAGAAGTCCGCGCGCAATTTCGGCTCGGTGCTGATCGGCATCGAAACCAAG
GACCGTCGCAATTTCGACCTGCTCAAGGCAAATTTCGAGGCCGAGGGCGTCCAGTATCAGGACATCACCGACAACGAGAC
ATTGGCGGGCTTCATCATATGA

Upstream 100 bases:

>100_bases
TAAGCGGTCGGCCAATGGCGTTGCGAGGCCGGATATGCAATAGGCAGGATCGGCTGCGAGAGATCAGCATGCAATAAACA
GACGTTGTGCGGCCAGGTTG

Downstream 100 bases:

>100_bases
GCGCAAAGGTTTTCATCGCTCTGTTTTCCGGCATCAATGTCGGCGGCAACCGCATCGTCAAGATGGCGGAACTGCGCTCC
TTCTTCGAGGATCTCGGCTT

Product: threonine dehydratase

Products: NA

Alternate protein names: Threonine deaminase [H]

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MTGGPRRHAAQGGFPMNSFSSRVATAAAAIRQIFPETPLQENDYLSKKTGARVLLKREDLSPVRSYKIRGAFNFFRKALG
EGNQAELFVCASAGNHAQGFAFVCRHFGKKGVVFMPVTTPQQKIDKTRLFGGDFVEIRLVGDFFDDCYRAAFEFTERSGA
HMVPPFDHKDIIEGQATVAYEIAAQMAGARMPDIVMLPVGGGGLAAGVTHYFADQGREARFVFCEPAGAPSLHDSLAAGK
RLKLAKVDNFVDGAAVAEIGREPLRFLKEFAADTVRLIPENRLCATMIEMLNVEGVVLEPAGALAIDALKDFSRKEIRGK
TIVAVVSGGNFDFERLPDVKERALRFEGLKKYFIIRFPQRPGALRDFLEMLGPDDDIARFEYLKKSARNFGSVLIGIETK
DRRNFDLLKANFEAEGVQYQDITDNETLAGFII

Sequences:

>Translated_433_residues
MTGGPRRHAAQGGFPMNSFSSRVATAAAAIRQIFPETPLQENDYLSKKTGARVLLKREDLSPVRSYKIRGAFNFFRKALG
EGNQAELFVCASAGNHAQGFAFVCRHFGKKGVVFMPVTTPQQKIDKTRLFGGDFVEIRLVGDFFDDCYRAAFEFTERSGA
HMVPPFDHKDIIEGQATVAYEIAAQMAGARMPDIVMLPVGGGGLAAGVTHYFADQGREARFVFCEPAGAPSLHDSLAAGK
RLKLAKVDNFVDGAAVAEIGREPLRFLKEFAADTVRLIPENRLCATMIEMLNVEGVVLEPAGALAIDALKDFSRKEIRGK
TIVAVVSGGNFDFERLPDVKERALRFEGLKKYFIIRFPQRPGALRDFLEMLGPDDDIARFEYLKKSARNFGSVLIGIETK
DRRNFDLLKANFEAEGVQYQDITDNETLAGFII
>Mature_432_residues
TGGPRRHAAQGGFPMNSFSSRVATAAAAIRQIFPETPLQENDYLSKKTGARVLLKREDLSPVRSYKIRGAFNFFRKALGE
GNQAELFVCASAGNHAQGFAFVCRHFGKKGVVFMPVTTPQQKIDKTRLFGGDFVEIRLVGDFFDDCYRAAFEFTERSGAH
MVPPFDHKDIIEGQATVAYEIAAQMAGARMPDIVMLPVGGGGLAAGVTHYFADQGREARFVFCEPAGAPSLHDSLAAGKR
LKLAKVDNFVDGAAVAEIGREPLRFLKEFAADTVRLIPENRLCATMIEMLNVEGVVLEPAGALAIDALKDFSRKEIRGKT
IVAVVSGGNFDFERLPDVKERALRFEGLKKYFIIRFPQRPGALRDFLEMLGPDDDIARFEYLKKSARNFGSVLIGIETKD
RRNFDLLKANFEAEGVQYQDITDNETLAGFII

Specific function: Catalyzes the formation of alpha-ketobutyrate from threonine in a two-step reaction. The first step is a dehydration of threonine, followed by rehydration and liberation of ammonia [H]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the serine/threonine dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI11345492, Length=309, Percent_Identity=25.5663430420712, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI33469958, Length=318, Percent_Identity=27.9874213836478, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI19923959, Length=304, Percent_Identity=25.3289473684211, Blast_Score=82, Evalue=8e-16,
Organism=Escherichia coli, GI1790207, Length=362, Percent_Identity=35.3591160220994, Blast_Score=178, Evalue=6e-46,
Organism=Escherichia coli, GI1789505, Length=305, Percent_Identity=30.4918032786885, Blast_Score=110, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71991565, Length=347, Percent_Identity=28.2420749279539, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17508781, Length=284, Percent_Identity=26.4084507042254, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17537387, Length=400, Percent_Identity=28, Blast_Score=92, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6320930, Length=412, Percent_Identity=32.5242718446602, Blast_Score=195, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6322631, Length=313, Percent_Identity=25.2396166134185, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6319788, Length=290, Percent_Identity=23.1034482758621, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21355833, Length=349, Percent_Identity=26.3610315186246, Blast_Score=75, Evalue=7e-14,

Paralogues:

None

Copy number: 1344 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR001721
- InterPro:   IPR011820 [H]

Pfam domain/function: PF00291 PALP; PF00585 Thr_dehydrat_C [H]

EC number: =4.3.1.19 [H]

Molecular weight: Translated: 47808; Mature: 47677

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGGPRRHAAQGGFPMNSFSSRVATAAAAIRQIFPETPLQENDYLSKKTGARVLLKREDL
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCC
SPVRSYKIRGAFNFFRKALGEGNQAELFVCASAGNHAQGFAFVCRHFGKKGVVFMPVTTP
CHHHHEEHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCC
QQKIDKTRLFGGDFVEIRLVGDFFDDCYRAAFEFTERSGAHMVPPFDHKDIIEGQATVAY
HHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCHHHHH
EIAAQMAGARMPDIVMLPVGGGGLAAGVTHYFADQGREARFVFCEPAGAPSLHDSLAAGK
HHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHCCC
RLKLAKVDNFVDGAAVAEIGREPLRFLKEFAADTVRLIPENRLCATMIEMLNVEGVVLEP
EEEEEHHCCCCCHHHHHHHCHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHCCCCEEECC
AGALAIDALKDFSRKEIRGKTIVAVVSGGNFDFERLPDVKERALRFEGLKKYFIIRFPQR
CCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHCCCHHHHHHHHHCCCEEEEEECCCC
PGALRDFLEMLGPDDDIARFEYLKKSARNFGSVLIGIETKDRRNFDLLKANFEAEGVQYQ
CCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEECCCCCCEEEE
DITDNETLAGFII
CCCCCCCEEEEEC
>Mature Secondary Structure 
TGGPRRHAAQGGFPMNSFSSRVATAAAAIRQIFPETPLQENDYLSKKTGARVLLKREDL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCC
SPVRSYKIRGAFNFFRKALGEGNQAELFVCASAGNHAQGFAFVCRHFGKKGVVFMPVTTP
CHHHHEEHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCC
QQKIDKTRLFGGDFVEIRLVGDFFDDCYRAAFEFTERSGAHMVPPFDHKDIIEGQATVAY
HHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCHHHHH
EIAAQMAGARMPDIVMLPVGGGGLAAGVTHYFADQGREARFVFCEPAGAPSLHDSLAAGK
HHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHCCC
RLKLAKVDNFVDGAAVAEIGREPLRFLKEFAADTVRLIPENRLCATMIEMLNVEGVVLEP
EEEEEHHCCCCCHHHHHHHCHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHCCCCEEECC
AGALAIDALKDFSRKEIRGKTIVAVVSGGNFDFERLPDVKERALRFEGLKKYFIIRFPQR
CCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHCCCHHHHHHHHHCCCEEEEEECCCC
PGALRDFLEMLGPDDDIARFEYLKKSARNFGSVLIGIETKDRRNFDLLKANFEAEGVQYQ
CCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEECCCCCCEEEE
DITDNETLAGFII
CCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]