Click here to switch to the map view.

The map label for this gene is rutF [H]

Identifier: 13470391

GI number: 13470391

Start: 60308

End: 60835

Strand: Direct

Name: rutF [H]

Synonym: mlr0087

Alternate gene names: 13470391

Gene position: 60308-60835 (Clockwise)

Preceding gene: 13470388

Following gene: 13470392

Centisome position: 0.86

GC content: 61.36

Gene sequence:

>528_bases
GTGCTGAAGAAGAATGATATTGGACCGCAGGCCTATCGCGACGCGATGAGCCATTTTGCCGGCCACGTCCATGTGGTCAC
CACCGACGGGCCCGCCGGCAAGCGCGGCACGACGGTGATCGCTGCCTGCTCGGTGTCGGACACGCCGCCGACCATTCTGG
TCTGTCTCAATCGCGAAAATGCCAATAATGAGCTGTTCGTGAAAAACGGCCGGTTTGCCCTGAACACGCTGGCTTCGCAC
CAGGAACCGCTGTCGATCGGCTTTTCAGGCATCACCGGCCTGCCGGTCGAGGAGCGTTTCGCGCTCGGCGAATGGGACGT
GATCTCCACCGGCGCGCCGACGCTGAAGGGCGCGCTCGCCGTGTTCGACTGCGAATTGATCGACACCAAGGATCTGGCCA
CGCATCGTGTGCTTTTTGGCAAGGTGACAGGCCTTCGCATGGGCGATAATTTGCGGCCGCTGATCTATCACGCCCGCGGC
TATCATGTCCTGGACAGCGGAGCGGCGGCGTTCACGGAGAAAGAATGA

Upstream 100 bases:

>100_bases
TGCAACACGCGAATTCGGGGATATGGGCCAAGCACTTCGCTTGTGGCATGTCATGTCTGGCCATATATAGCGGTAGGGTT
CTGGCTATGGACAATTTCCG

Downstream 100 bases:

>100_bases
CCGAACTGAAACCGCGCCCCGTACCGCGGACGATCGACGAGACGCTCGATCTCCTGACCGGCGCGGACTATGTGGCGGAC
CGGTCGCTGGCGACCGTGCT

Product: monooxygenase

Products: NA

Alternate protein names: FMN reductase; NAD(P)H:flavin oxidoreductase [H]

Number of amino acids: Translated: 175; Mature: 175

Protein sequence:

>175_residues
MLKKNDIGPQAYRDAMSHFAGHVHVVTTDGPAGKRGTTVIAACSVSDTPPTILVCLNRENANNELFVKNGRFALNTLASH
QEPLSIGFSGITGLPVEERFALGEWDVISTGAPTLKGALAVFDCELIDTKDLATHRVLFGKVTGLRMGDNLRPLIYHARG
YHVLDSGAAAFTEKE

Sequences:

>Translated_175_residues
MLKKNDIGPQAYRDAMSHFAGHVHVVTTDGPAGKRGTTVIAACSVSDTPPTILVCLNRENANNELFVKNGRFALNTLASH
QEPLSIGFSGITGLPVEERFALGEWDVISTGAPTLKGALAVFDCELIDTKDLATHRVLFGKVTGLRMGDNLRPLIYHARG
YHVLDSGAAAFTEKE
>Mature_175_residues
MLKKNDIGPQAYRDAMSHFAGHVHVVTTDGPAGKRGTTVIAACSVSDTPPTILVCLNRENANNELFVKNGRFALNTLASH
QEPLSIGFSGITGLPVEERFALGEWDVISTGAPTLKGALAVFDCELIDTKDLATHRVLFGKVTGLRMGDNLRPLIYHARG
YHVLDSGAAAFTEKE

Specific function: Catalyzes the reduction of FMN to FMNH2 which is used to reduce pyrimidine by RutA via the Rut pathway [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the non-flavoprotein flavin reductase family. RutF subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081819, Length=160, Percent_Identity=43.125, Blast_Score=132, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 18817; Mature: 18817

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKNDIGPQAYRDAMSHFAGHVHVVTTDGPAGKRGTTVIAACSVSDTPPTILVCLNREN
CCCCCCCCHHHHHHHHHHHCCEEEEEECCCCCCCCCCEEEEEEECCCCCCEEEEEECCCC
ANNELFVKNGRFALNTLASHQEPLSIGFSGITGLPVEERFALGEWDVISTGAPTLKGALA
CCCEEEEECCCEEEEEHHCCCCCEEECCCCCCCCCCHHHEECCCCEEEECCCCCHHCEEE
VFDCELIDTKDLATHRVLFGKVTGLRMGDNLRPLIYHARGYHVLDSGAAAFTEKE
EEEEEEECCCCCHHHEEEEEEEECEEECCCCCEEEEECCCCEEEECCCCCCCCCC
>Mature Secondary Structure
MLKKNDIGPQAYRDAMSHFAGHVHVVTTDGPAGKRGTTVIAACSVSDTPPTILVCLNREN
CCCCCCCCHHHHHHHHHHHCCEEEEEECCCCCCCCCCEEEEEEECCCCCCEEEEEECCCC
ANNELFVKNGRFALNTLASHQEPLSIGFSGITGLPVEERFALGEWDVISTGAPTLKGALA
CCCEEEEECCCEEEEEHHCCCCCEEECCCCCCCCCCHHHEECCCCEEEECCCCCHHCEEE
VFDCELIDTKDLATHRVLFGKVTGLRMGDNLRPLIYHARGYHVLDSGAAAFTEKE
EEEEEEECCCCCHHHEEEEEEEECEEECCCCCEEEEECCCCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA