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The map label for this gene is bcr [H]

Identifier: 13470366

GI number: 13470366

Start: 39311

End: 40495

Strand: Reverse

Name: bcr [H]

Synonym: mll0051

Alternate gene names: 13470366

Gene position: 40495-39311 (Counterclockwise)

Preceding gene: 13470367

Following gene: 13470362

Centisome position: 0.58

GC content: 62.62

Gene sequence:

>1185_bases
ATGGCGCTGAATTCGCTGGCCATCGACATCATGCTGCCGGCGCTGCAGCAGATCGGCGCCTCGCTCGGCGTCGAGAATGA
AAACCACCGGCAATATGTGGTCACCGCCTACATTCTGGGTTTTGGTGGCGGCCAGCTGTTCTTCGGACCGATCTCCGACC
GGTTCGGCCGCCGCTCGCCGCTCGTCGCCGGGCTGATCATCTATGTGGCGGCGGCCGCCGCCGCCGCCATCGCGCCAAGC
TTCGAAACGCTGCTGATATGCCGTGCCGTGCAAGGCATCGGCGCTGCCGCCACGCGCGTCATCGCGGTCTCGATCGTGCG
TGATACGTTCGAAGGCCGGCGCATGGCGGAGGTCATGTCGCTGATCTTCATGGTGTTCATGGCCATACCGGTCATTGCGC
CGGGCATCGGCCAGTTCATCATGCTGTTTGCGACCTGGCACTACATCTTCGTCACCATGGCCGTCGGCGCGCTGATCGTG
TCGGCGTGGTCGCTGCTGCGCCTGCCTGAAACGCTGCGTCCCGAATATCGCCGGCAACTGACGGTTTCCTCCGTCCTCGG
CGGATTCCGCATCGTGCTCACCAACCGCATCGCGCTCTGCTACGCCTTTGCCAGCACCTTCATCTTCGCCGCCATGTTCG
GCTTCATCGCCTCGGCGCAGCAGATCTATGTCGGCATCTTCAACGTCGGCGAAATGTTCCCGGTCATTTTCGCCGGGGTC
GCCGGCGTGCTCGCCTTCTCCAATTTTCTGAACTCGCGCCTTGTCGGCCGCATCGGCATGCGCCGTCTGTCGCAAAGTGC
GTTGCTTTTGTTTCTGGTCATCAGCCTGGCTTGGCTGGTCGTTTCGCTGGAAATCAAGATGCCGCTCTGGCTGTTCATCA
CCTTCTTTGCCAGCGCCATGCTTCCCTTCGGCGCGCTTGGCGCCAATTTCAACGCCTTGGCGATGGAACCGCTCGGCCAG
CTGGCCGGCACGGCATCGTCCATACTGGGCTTCATGCAGACGTTTCTCGGCGGCATTCTCGGCACGCTGATCGGCCAGGC
CTTCAACGGCACGGTGACGCCGCTTGCCGCCGGCTTCTGCAGCGTCTCGGTCGCCGCGCTGCTGATGATCCTCATCGCCG
AGCGCGGCAAGATGTTCCAGCCGCATAACCCACCCATTTCAGGGCATATCACCGACCTGCATTAG

Upstream 100 bases:

>100_bases
GAAGATCGGACTTGCCGTGGACCAGCAATCAACAGCGCCGCAATCGGCAAGCAAACTGCCTATTCCGCGCTGGGAGTTTA
TTGCGCTGTGCGCGGCACTG

Downstream 100 bases:

>100_bases
ACGATGTGCGTCAGGATGCACCTTCAAGCGCCGCCGTGAAGCGAGGCGCAAGCATGCGTCCAGCAATTGCTCCGCCCATG
CCGTCCGAAAAGGGCAATGG

Product: bicyclomycin resistance protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MALNSLAIDIMLPALQQIGASLGVENENHRQYVVTAYILGFGGGQLFFGPISDRFGRRSPLVAGLIIYVAAAAAAAIAPS
FETLLICRAVQGIGAAATRVIAVSIVRDTFEGRRMAEVMSLIFMVFMAIPVIAPGIGQFIMLFATWHYIFVTMAVGALIV
SAWSLLRLPETLRPEYRRQLTVSSVLGGFRIVLTNRIALCYAFASTFIFAAMFGFIASAQQIYVGIFNVGEMFPVIFAGV
AGVLAFSNFLNSRLVGRIGMRRLSQSALLLFLVISLAWLVVSLEIKMPLWLFITFFASAMLPFGALGANFNALAMEPLGQ
LAGTASSILGFMQTFLGGILGTLIGQAFNGTVTPLAAGFCSVSVAALLMILIAERGKMFQPHNPPISGHITDLH

Sequences:

>Translated_394_residues
MALNSLAIDIMLPALQQIGASLGVENENHRQYVVTAYILGFGGGQLFFGPISDRFGRRSPLVAGLIIYVAAAAAAAIAPS
FETLLICRAVQGIGAAATRVIAVSIVRDTFEGRRMAEVMSLIFMVFMAIPVIAPGIGQFIMLFATWHYIFVTMAVGALIV
SAWSLLRLPETLRPEYRRQLTVSSVLGGFRIVLTNRIALCYAFASTFIFAAMFGFIASAQQIYVGIFNVGEMFPVIFAGV
AGVLAFSNFLNSRLVGRIGMRRLSQSALLLFLVISLAWLVVSLEIKMPLWLFITFFASAMLPFGALGANFNALAMEPLGQ
LAGTASSILGFMQTFLGGILGTLIGQAFNGTVTPLAAGFCSVSVAALLMILIAERGKMFQPHNPPISGHITDLH
>Mature_393_residues
ALNSLAIDIMLPALQQIGASLGVENENHRQYVVTAYILGFGGGQLFFGPISDRFGRRSPLVAGLIIYVAAAAAAAIAPSF
ETLLICRAVQGIGAAATRVIAVSIVRDTFEGRRMAEVMSLIFMVFMAIPVIAPGIGQFIMLFATWHYIFVTMAVGALIVS
AWSLLRLPETLRPEYRRQLTVSSVLGGFRIVLTNRIALCYAFASTFIFAAMFGFIASAQQIYVGIFNVGEMFPVIFAGVA
GVLAFSNFLNSRLVGRIGMRRLSQSALLLFLVISLAWLVVSLEIKMPLWLFITFFASAMLPFGALGANFNALAMEPLGQL
AGTASSILGFMQTFLGGILGTLIGQAFNGTVTPLAAGFCSVSVAALLMILIAERGKMFQPHNPPISGHITDLH

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=378, Percent_Identity=32.5396825396825, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI1790146, Length=391, Percent_Identity=29.156010230179, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI87082312, Length=354, Percent_Identity=27.683615819209, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1790794, Length=192, Percent_Identity=30.2083333333333, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI48994889, Length=148, Percent_Identity=30.4054054054054, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1787065, Length=236, Percent_Identity=26.6949152542373, Blast_Score=65, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6324264, Length=171, Percent_Identity=31.5789473684211, Blast_Score=64, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42260; Mature: 42129

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALNSLAIDIMLPALQQIGASLGVENENHRQYVVTAYILGFGGGQLFFGPISDRFGRRSP
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEHCCCCCCEEECCHHHHCCCCCH
LVAGLIIYVAAAAAAAIAPSFETLLICRAVQGIGAAATRVIAVSIVRDTFEGRRMAEVMS
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHH
LIFMVFMAIPVIAPGIGQFIMLFATWHYIFVTMAVGALIVSAWSLLRLPETLRPEYRRQL
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCHHHHHHH
TVSSVLGGFRIVLTNRIALCYAFASTFIFAAMFGFIASAQQIYVGIFNVGEMFPVIFAGV
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGVLAFSNFLNSRLVGRIGMRRLSQSALLLFLVISLAWLVVSLEIKMPLWLFITFFASAM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LPFGALGANFNALAMEPLGQLAGTASSILGFMQTFLGGILGTLIGQAFNGTVTPLAAGFC
CCHHHCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
SVSVAALLMILIAERGKMFQPHNPPISGHITDLH
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ALNSLAIDIMLPALQQIGASLGVENENHRQYVVTAYILGFGGGQLFFGPISDRFGRRSP
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEHCCCCCCEEECCHHHHCCCCCH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HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]