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The map label for this gene is sacA [H]

Identifier: 134302766

GI number: 134302766

Start: 1855946

End: 1857658

Strand: Reverse

Name: sacA [H]

Synonym: FTW_1977

Alternate gene names: 134302766

Gene position: 1857658-1855946 (Counterclockwise)

Preceding gene: 134302768

Following gene: 134302765

Centisome position: 97.85

GC content: 31.29

Gene sequence:

>1713_bases
ATGTGTTTAACAAAATACTCTCGGATTAACTATATCAATATTAAAAATAACTCTGTAATCTCTTTTGACTATAACTCTGT
CAATCAGGGTCAATTTAGACTAAAACATAATGACAATATTGTTGAATGTGTCGCGATAATACCGACAAACCTAAAATCAA
AATATTACCATGTTGAATGGTCTTTAGTAGCTGATCAAGAATATATTTTCGAAATAGTTGGAGATATACAAAACTTGAAT
ATTCAAAATGGTTGGATATATGATGCTAACTTACTTGAGACAGGTGGTATGTGCTTTAGCTTACGGGCGGATAGCTTTAA
ATCTTACCATTCGCATGATAATAGTGATATACATAAAACTAAACGCTTGCAGTACCATTTTACTGCTAAAAAGGCTTGGA
TGAATGATCCAAACGGTTTAGTTTACTTTAAAGGTAAATATCATATGTTTTACCAGCACTACCCTTATGGTAAACATACA
GCAATGCTACACTGGGGACATGCTGTATCTACAGACCTTGTCAACTGGCAACATTTACCTATTGCAATTTATCCACAAAA
TGATCTTAATGATAGATATATCGGTGGCGCTTTTTCCGATAGTGCTATGGTTGTCGATGATGAGCTTTTTCTTATTTATA
CCGAACATTTTGAAGATTTAGACAACACTCCAAATATCTTTATAGAAAAACAAAATCTAATAAAAAGCAAAGATGGTATT
CACTTCTCAAAACCGAAAACAGTAATAAATCATAAGCCAGATTTTTGTTCTTATGACTTTAGAGATCCTAAAGTATGGTT
TGATAAGATTTTTAACTGTTATTACATGGTGATTGGCACTTATGCAAATAACTATCCAAGTGTTGCACTGTATAGATCTG
ATAATGCTGAGAGCTGGAATTATCATAGTATATTATTTCAAGAAAAAACTATCTCTGGAAGAACTCTAGAATGCCTAGAC
CTGTTTTATTTAGATGGAAAATATGTATTGGTTTTATCAATTTTTGAGACAAAAAATAAGAAGGACTTTAATTATCAGTC
ATATTATTATATTGGAAACTTCGATGGTAAGCATTTCTCTGCAGAAACACCTCCTAAGCATATAGACTCTGCAAAAGAGT
TTTATGCACCACAGACTTTTGAAGCTAATGGTAATAGATACGCTATCGGTTGGATGAACTGTTGGGAAGACTATAAAAAC
CGAGCTAAAGACGATTCGGCTGGGGCAATGAGTTTAATCAGACAGCTTAAAGTAAAGAAAGATAAGCTTTTATCTTATCC
TGTAGATGACTATAAAAACCTTAGAAATAAGACTATAGAAATCAAAAACTTTGATCAAGACATTACTCTGCCTTCAAATC
TAATAGAACTAAATTTAGATTTTAGCTCGGATAATAACTGGCAACTAGATCTTTTAGAGAATAATCAAGGATTTGTGATT
AATATTCATTATCAAGATAATCAGATATTAGTGAAAAATAGCCTTAATGAAGTTATAGATAAAGCTTTTATAAAAGATAT
CCGCTCGCAAGAAATATCTGTAGATATTTTCTTAGATACAACTAGTATCGAGCTTTTTGTTAATAAGGGAGAAGAATCAA
TTACTATGAGATTTGAAGCATCTGATACAATAAAAAATACTATGAGAGTATCAGCTGTAAACCAAGATGATCTAACTATC
AAATTATATGAACTAAATTCAGTATGGAAATAA

Upstream 100 bases:

>100_bases
TCTCCAGATTTAAATCCTATTGAAAAGGTTTGGGCTAACTTTAAAAAAATATTTAGAAAAGTGAATAATAGTTTTGAAAA
ATTTTGTGATGCTATCTCTT

Downstream 100 bases:

>100_bases
AAAACAGAGAATTTACAGATGGCATCAATTAATACAAATAACCAGTTATCTACCTTTATTGTTCCTGATAATTTAAAACT
AAAAGCTGTTAGTATCTGCT

Product: glycosy hydrolase family protein

Products: NA

Alternate protein names: Sucrase; Invertase [H]

Number of amino acids: Translated: 570; Mature: 570

Protein sequence:

>570_residues
MCLTKYSRINYINIKNNSVISFDYNSVNQGQFRLKHNDNIVECVAIIPTNLKSKYYHVEWSLVADQEYIFEIVGDIQNLN
IQNGWIYDANLLETGGMCFSLRADSFKSYHSHDNSDIHKTKRLQYHFTAKKAWMNDPNGLVYFKGKYHMFYQHYPYGKHT
AMLHWGHAVSTDLVNWQHLPIAIYPQNDLNDRYIGGAFSDSAMVVDDELFLIYTEHFEDLDNTPNIFIEKQNLIKSKDGI
HFSKPKTVINHKPDFCSYDFRDPKVWFDKIFNCYYMVIGTYANNYPSVALYRSDNAESWNYHSILFQEKTISGRTLECLD
LFYLDGKYVLVLSIFETKNKKDFNYQSYYYIGNFDGKHFSAETPPKHIDSAKEFYAPQTFEANGNRYAIGWMNCWEDYKN
RAKDDSAGAMSLIRQLKVKKDKLLSYPVDDYKNLRNKTIEIKNFDQDITLPSNLIELNLDFSSDNNWQLDLLENNQGFVI
NIHYQDNQILVKNSLNEVIDKAFIKDIRSQEISVDIFLDTTSIELFVNKGEESITMRFEASDTIKNTMRVSAVNQDDLTI
KLYELNSVWK

Sequences:

>Translated_570_residues
MCLTKYSRINYINIKNNSVISFDYNSVNQGQFRLKHNDNIVECVAIIPTNLKSKYYHVEWSLVADQEYIFEIVGDIQNLN
IQNGWIYDANLLETGGMCFSLRADSFKSYHSHDNSDIHKTKRLQYHFTAKKAWMNDPNGLVYFKGKYHMFYQHYPYGKHT
AMLHWGHAVSTDLVNWQHLPIAIYPQNDLNDRYIGGAFSDSAMVVDDELFLIYTEHFEDLDNTPNIFIEKQNLIKSKDGI
HFSKPKTVINHKPDFCSYDFRDPKVWFDKIFNCYYMVIGTYANNYPSVALYRSDNAESWNYHSILFQEKTISGRTLECLD
LFYLDGKYVLVLSIFETKNKKDFNYQSYYYIGNFDGKHFSAETPPKHIDSAKEFYAPQTFEANGNRYAIGWMNCWEDYKN
RAKDDSAGAMSLIRQLKVKKDKLLSYPVDDYKNLRNKTIEIKNFDQDITLPSNLIELNLDFSSDNNWQLDLLENNQGFVI
NIHYQDNQILVKNSLNEVIDKAFIKDIRSQEISVDIFLDTTSIELFVNKGEESITMRFEASDTIKNTMRVSAVNQDDLTI
KLYELNSVWK
>Mature_570_residues
MCLTKYSRINYINIKNNSVISFDYNSVNQGQFRLKHNDNIVECVAIIPTNLKSKYYHVEWSLVADQEYIFEIVGDIQNLN
IQNGWIYDANLLETGGMCFSLRADSFKSYHSHDNSDIHKTKRLQYHFTAKKAWMNDPNGLVYFKGKYHMFYQHYPYGKHT
AMLHWGHAVSTDLVNWQHLPIAIYPQNDLNDRYIGGAFSDSAMVVDDELFLIYTEHFEDLDNTPNIFIEKQNLIKSKDGI
HFSKPKTVINHKPDFCSYDFRDPKVWFDKIFNCYYMVIGTYANNYPSVALYRSDNAESWNYHSILFQEKTISGRTLECLD
LFYLDGKYVLVLSIFETKNKKDFNYQSYYYIGNFDGKHFSAETPPKHIDSAKEFYAPQTFEANGNRYAIGWMNCWEDYKN
RAKDDSAGAMSLIRQLKVKKDKLLSYPVDDYKNLRNKTIEIKNFDQDITLPSNLIELNLDFSSDNNWQLDLLENNQGFVI
NIHYQDNQILVKNSLNEVIDKAFIKDIRSQEISVDIFLDTTSIELFVNKGEESITMRFEASDTIKNTMRVSAVNQDDLTI
KLYELNSVWK

Specific function: Unknown

COG id: COG1621

COG function: function code G; Beta-fructosidases (levanase/invertase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 32 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6322029, Length=457, Percent_Identity=26.9146608315098, Blast_Score=109, Evalue=9e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008985
- InterPro:   IPR001362
- InterPro:   IPR018053
- InterPro:   IPR013189
- InterPro:   IPR013148
- InterPro:   IPR006232 [H]

Pfam domain/function: PF08244 Glyco_hydro_32C; PF00251 Glyco_hydro_32N [H]

EC number: =3.2.1.26 [H]

Molecular weight: Translated: 66800; Mature: 66800

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCLTKYSRINYINIKNNSVISFDYNSVNQGQFRLKHNDNIVECVAIIPTNLKSKYYHVEW
CCCEEECCEEEEEECCCEEEEEECCCCCCCEEEEECCCCEEEEEEEECCCCCCEEEEEEE
SLVADQEYIFEIVGDIQNLNIQNGWIYDANLLETGGMCFSLRADSFKSYHSHDNSDIHKT
EEEECHHHHHHHHCCHHCCCCCCCEEEECCEEECCCEEEEEECCCHHHHCCCCCCCCCHH
KRLQYHFTAKKAWMNDPNGLVYFKGKYHMFYQHYPYGKHTAMLHWGHAVSTDLVNWQHLP
EEEEEEEEEHHHHCCCCCCEEEECCEEEEEEEECCCCCEEEEEEECCCCCCCCCCEEECE
IAIYPQNDLNDRYIGGAFSDSAMVVDDELFLIYTEHFEDLDNTPNIFIEKQNLIKSKDGI
EEEECCCCCCCCEECCCCCCCEEEEECCEEEEEECHHHCCCCCCCEEEECCHHHCCCCCE
HFSKPKTVINHKPDFCSYDFRDPKVWFDKIFNCYYMVIGTYANNYPSVALYRSDNAESWN
EECCCCCEECCCCCCCCCCCCCCHHHHHHHHHEEEEEEEECCCCCCCEEEEECCCCCCCE
YHSILFQEKTISGRTLECLDLFYLDGKYVLVLSIFETKNKKDFNYQSYYYIGNFDGKHFS
EEEEEEEECCCCCCEEEEEEEEEECCCEEEEEEEEECCCCCCCCEEEEEEEECCCCCCCC
AETPPKHIDSAKEFYAPQTFEANGNRYAIGWMNCWEDYKNRAKDDSAGAMSLIRQLKVKK
CCCCHHHHCCHHHHCCCCEEECCCCEEEEEHHHHHHHHHHCCCCCCHHHHHHHHHHHCCH
DKLLSYPVDDYKNLRNKTIEIKNFDQDITLPSNLIELNLDFSSDNNWQLDLLENNQGFVI
HHHCCCCCHHHHHHCCCEEEEEECCCCCCCCCCEEEEEEEECCCCCEEEEEEECCCCEEE
NIHYQDNQILVKNSLNEVIDKAFIKDIRSQEISVDIFLDTTSIELFVNKGEESITMRFEA
EEEECCCEEEEECCHHHHHHHHHHHHHCCCEEEEEEEEECCEEEEEEECCCCEEEEEEEC
SDTIKNTMRVSAVNQDDLTIKLYELNSVWK
CHHHCCCEEEEEECCCCCEEEEEECCCCCC
>Mature Secondary Structure
MCLTKYSRINYINIKNNSVISFDYNSVNQGQFRLKHNDNIVECVAIIPTNLKSKYYHVEW
CCCEEECCEEEEEECCCEEEEEECCCCCCCEEEEECCCCEEEEEEEECCCCCCEEEEEEE
SLVADQEYIFEIVGDIQNLNIQNGWIYDANLLETGGMCFSLRADSFKSYHSHDNSDIHKT
EEEECHHHHHHHHCCHHCCCCCCCEEEECCEEECCCEEEEEECCCHHHHCCCCCCCCCHH
KRLQYHFTAKKAWMNDPNGLVYFKGKYHMFYQHYPYGKHTAMLHWGHAVSTDLVNWQHLP
EEEEEEEEEHHHHCCCCCCEEEECCEEEEEEEECCCCCEEEEEEECCCCCCCCCCEEECE
IAIYPQNDLNDRYIGGAFSDSAMVVDDELFLIYTEHFEDLDNTPNIFIEKQNLIKSKDGI
EEEECCCCCCCCEECCCCCCCEEEEECCEEEEEECHHHCCCCCCCEEEECCHHHCCCCCE
HFSKPKTVINHKPDFCSYDFRDPKVWFDKIFNCYYMVIGTYANNYPSVALYRSDNAESWN
EECCCCCEECCCCCCCCCCCCCCHHHHHHHHHEEEEEEEECCCCCCCEEEEECCCCCCCE
YHSILFQEKTISGRTLECLDLFYLDGKYVLVLSIFETKNKKDFNYQSYYYIGNFDGKHFS
EEEEEEEECCCCCCEEEEEEEEEECCCEEEEEEEEECCCCCCCCEEEEEEEECCCCCCCC
AETPPKHIDSAKEFYAPQTFEANGNRYAIGWMNCWEDYKNRAKDDSAGAMSLIRQLKVKK
CCCCHHHHCCHHHHCCCCEEECCCCEEEEEHHHHHHHHHHCCCCCCHHHHHHHHHHHCCH
DKLLSYPVDDYKNLRNKTIEIKNFDQDITLPSNLIELNLDFSSDNNWQLDLLENNQGFVI
HHHCCCCCHHHHHHCCCEEEEEECCCCCCCCCCEEEEEEEECCCCCEEEEEEECCCCEEE
NIHYQDNQILVKNSLNEVIDKAFIKDIRSQEISVDIFLDTTSIELFVNKGEESITMRFEA
EEEECCCEEEEECCHHHHHHHHHHHHHCCCEEEEEEEEECCEEEEEEECCCCEEEEEEEC
SDTIKNTMRVSAVNQDDLTIKLYELNSVWK
CHHHCCCEEEEEECCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2254250; 1368686 [H]