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The map label for this gene is ykwC [H]

Identifier: 134302746

GI number: 134302746

Start: 1830820

End: 1831707

Strand: Direct

Name: ykwC [H]

Synonym: FTW_1953

Alternate gene names: 134302746

Gene position: 1830820-1831707 (Clockwise)

Preceding gene: 134302742

Following gene: 134302751

Centisome position: 96.44

GC content: 27.82

Gene sequence:

>888_bases
GTGGCTAAAATGAGTAAACAAGTTGGTATTATTGGTTTAGGAAATATGGGATCTGTTGTTATAGATCACCTGCTAACATC
AGGATATGAGATTTTTATTCATAATAGAACCAAAGCAAAGGCTGAAAAATGGTTAAGAAATACACAAGTAGTTTGGTGTG
ATTCTCCAAAAGAGTTAGCTGAAAATTGCGAAATAATAATTTGTTGTCTATATAATGATATTGCTGCAAAGAATGTTTAT
CTCAGTAAAAATGGCTTAACTTCTATAAATTTAGTAAATCATACAATATTAGATGCGAGCACTCTTTCTGTAAGCTGTTT
TGAGCAACTTAATAAAGTCGTTACCCAAAATAATGGTAAATTAATAGATATGCCAATATCTGGAAATCCTGCTGCATTAC
AGGAAAAAAGAGCTTTAATTATGCTTGGTGCAACAGAAGAAGAATGTAAGAGCTTTGTACATTTCTTAGAAGCGCTTAGT
AATAAAATATTATATTTAGATATTGCTGGGTCTGCACTTAAAGAAAAACTTTGTATTAATGAAATGCTTTTTACTCAGTG
GTTTAGTTATTGTGAGGCAATGCTATTAGCAGAAAATAATGGTATTAAAACAGAAGATTTTTTTAAAGCAATGTCACAAA
GTGTTATATCATCACCTCTTTTGGTACAAAGAAGTCAATATTTCTTTGGATATAATGTAAATCCTTCAGATTTAAATATG
ATTTTGAAAGATATAGATATAATCGAAGATTTTGTAAATAAATCAAAAACAAAAGTTACTTTATTACAAAAATTTAGAGA
AGTAGTTGAATATGCTATTAAACATGATTTTACAAATCAAGATTTTTATGGGTTATATAGAGCTTATAAAGAAATTTATA
AAAAATAA

Upstream 100 bases:

>100_bases
TTTGCAGTAAAAAAGTGTTAATCAGTTTTGTGAGGTACTAAAATTAAATATAAAGGCAAGAGAGAATGATCTAGTAGGGT
TTTTCTCTGTACCAAAATAG

Downstream 100 bases:

>100_bases
CAAATTTTATTTTTTAGGATATTACTAATAAAGCCAATAGCGTCATCCTAATCGGAATACTATATTTGATTTGCTCAAAA
TATTTCGCACACTCAAGCTC

Product: dehydrogenase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MAKMSKQVGIIGLGNMGSVVIDHLLTSGYEIFIHNRTKAKAEKWLRNTQVVWCDSPKELAENCEIIICCLYNDIAAKNVY
LSKNGLTSINLVNHTILDASTLSVSCFEQLNKVVTQNNGKLIDMPISGNPAALQEKRALIMLGATEEECKSFVHFLEALS
NKILYLDIAGSALKEKLCINEMLFTQWFSYCEAMLLAENNGIKTEDFFKAMSQSVISSPLLVQRSQYFFGYNVNPSDLNM
ILKDIDIIEDFVNKSKTKVTLLQKFREVVEYAIKHDFTNQDFYGLYRAYKEIYKK

Sequences:

>Translated_295_residues
MAKMSKQVGIIGLGNMGSVVIDHLLTSGYEIFIHNRTKAKAEKWLRNTQVVWCDSPKELAENCEIIICCLYNDIAAKNVY
LSKNGLTSINLVNHTILDASTLSVSCFEQLNKVVTQNNGKLIDMPISGNPAALQEKRALIMLGATEEECKSFVHFLEALS
NKILYLDIAGSALKEKLCINEMLFTQWFSYCEAMLLAENNGIKTEDFFKAMSQSVISSPLLVQRSQYFFGYNVNPSDLNM
ILKDIDIIEDFVNKSKTKVTLLQKFREVVEYAIKHDFTNQDFYGLYRAYKEIYKK
>Mature_294_residues
AKMSKQVGIIGLGNMGSVVIDHLLTSGYEIFIHNRTKAKAEKWLRNTQVVWCDSPKELAENCEIIICCLYNDIAAKNVYL
SKNGLTSINLVNHTILDASTLSVSCFEQLNKVVTQNNGKLIDMPISGNPAALQEKRALIMLGATEEECKSFVHFLEALSN
KILYLDIAGSALKEKLCINEMLFTQWFSYCEAMLLAENNGIKTEDFFKAMSQSVISSPLLVQRSQYFFGYNVNPSDLNMI
LKDIDIIEDFVNKSKTKVTLLQKFREVVEYAIKHDFTNQDFYGLYRAYKEIYKK

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI40556376, Length=292, Percent_Identity=26.027397260274, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI23308751, Length=300, Percent_Identity=23.6666666666667, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI145693186, Length=290, Percent_Identity=20.3448275862069, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1790315, Length=277, Percent_Identity=22.3826714801444, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1786719, Length=245, Percent_Identity=23.265306122449, Blast_Score=64, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24655230, Length=301, Percent_Identity=24.2524916943522, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI19922568, Length=301, Percent_Identity=24.2524916943522, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI28574115, Length=283, Percent_Identity=21.5547703180212, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 33510; Mature: 33379

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: PS00109 PROTEIN_KINASE_TYR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKMSKQVGIIGLGNMGSVVIDHLLTSGYEIFIHNRTKAKAEKWLRNTQVVWCDSPKELA
CCCCCCCCCEEEECCHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHCCEEEEECCHHHHH
ENCEIIICCLYNDIAAKNVYLSKNGLTSINLVNHTILDASTLSVSCFEQLNKVVTQNNGK
CCCCEEEEEEECCHHHCEEEEECCCCCEEEEHHHHEECCCHHHHHHHHHHHHHHHCCCCE
LIDMPISGNPAALQEKRALIMLGATEEECKSFVHFLEALSNKILYLDIAGSALKEKLCIN
EEEECCCCCCHHHHCCCEEEEEECCHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHH
EMLFTQWFSYCEAMLLAENNGIKTEDFFKAMSQSVISSPLLVQRSQYFFGYNVNPSDLNM
HHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHCCCHHEECCCEEEECCCCHHHHHH
ILKDIDIIEDFVNKSKTKVTLLQKFREVVEYAIKHDFTNQDFYGLYRAYKEIYKK
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKMSKQVGIIGLGNMGSVVIDHLLTSGYEIFIHNRTKAKAEKWLRNTQVVWCDSPKELA
CCCCCCCCEEEECCHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHCCEEEEECCHHHHH
ENCEIIICCLYNDIAAKNVYLSKNGLTSINLVNHTILDASTLSVSCFEQLNKVVTQNNGK
CCCCEEEEEEECCHHHCEEEEECCCCCEEEEHHHHEECCCHHHHHHHHHHHHHHHCCCCE
LIDMPISGNPAALQEKRALIMLGATEEECKSFVHFLEALSNKILYLDIAGSALKEKLCIN
EEEECCCCCCHHHHCCCEEEEEECCHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHH
EMLFTQWFSYCEAMLLAENNGIKTEDFFKAMSQSVISSPLLVQRSQYFFGYNVNPSDLNM
HHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHCCCHHEECCCEEEECCCCHHHHHH
ILKDIDIIEDFVNKSKTKVTLLQKFREVVEYAIKHDFTNQDFYGLYRAYKEIYKK
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]