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The map label for this gene is def2 [H]

Identifier: 134302737

GI number: 134302737

Start: 1821855

End: 1822379

Strand: Reverse

Name: def2 [H]

Synonym: FTW_1942

Alternate gene names: 134302737

Gene position: 1822379-1821855 (Counterclockwise)

Preceding gene: 134302738

Following gene: 134302736

Centisome position: 95.99

GC content: 33.33

Gene sequence:

>525_bases
ATGAATATGTCTTTAGAAATTCTAAAGTACCCTCACCCAGTTTTAAAAGAGGTTGCTAAAGAAGTTACAAAAGATGAAAT
CAATGATGATTTACGTGCAACTATTGCTGAAATGCATGAGCTAATGCTAGAAGCAAATGGCGTGGGTTTAGCAGCAATAC
AAGTTGGTATCAAAAAAAGATTCTTTATCATGTATGATAACTTAGAAGAGCAAAACCCTGAAATAATCACTATCATTAAC
CCTGAAATAATTGAACAAAACGGTAAAATAATTGATGAAGAGGGCTGTCTTTCTTTTCCTGGTGTTTCCGCAAAGGTAAA
TAGAGCCACTGTAGTCAAAATAAAGGCACTTAATGAATTTGGAGAAGAGATTGAAGTTGAAAAAGATGGATTTTTAGCTA
GATGCATCCAACATGAGATAGATCATCTTAATGGCATAACCTTCTTTGACCATCTTGGTTCACTAAAGCGCAAAATGATA
GAGAAAAAGTATAAAAAACTAATGCAAGAAAATGCTAAGAGTTAA

Upstream 100 bases:

>100_bases
TAGAAAGAGTCGGTGGCAAAAAAGGTCATAAAGGTAAATACTCTATCCAAGCTGCCATAACGATGGCAAAGATGAAAAAA
GATATTTTAGAACAAGGAGT

Downstream 100 bases:

>100_bases
TTTTTGATTATTTTGTATAATCAATACTTTACTTTTTAGCAGCATTACGCAAAAATTATCTCCTGTAATTGTTATTAAAT
CATGGAATTTAGTATGCGCA

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 174; Mature: 174

Protein sequence:

>174_residues
MNMSLEILKYPHPVLKEVAKEVTKDEINDDLRATIAEMHELMLEANGVGLAAIQVGIKKRFFIMYDNLEEQNPEIITIIN
PEIIEQNGKIIDEEGCLSFPGVSAKVNRATVVKIKALNEFGEEIEVEKDGFLARCIQHEIDHLNGITFFDHLGSLKRKMI
EKKYKKLMQENAKS

Sequences:

>Translated_174_residues
MNMSLEILKYPHPVLKEVAKEVTKDEINDDLRATIAEMHELMLEANGVGLAAIQVGIKKRFFIMYDNLEEQNPEIITIIN
PEIIEQNGKIIDEEGCLSFPGVSAKVNRATVVKIKALNEFGEEIEVEKDGFLARCIQHEIDHLNGITFFDHLGSLKRKMI
EKKYKKLMQENAKS
>Mature_174_residues
MNMSLEILKYPHPVLKEVAKEVTKDEINDDLRATIAEMHELMLEANGVGLAAIQVGIKKRFFIMYDNLEEQNPEIITIIN
PEIIEQNGKIIDEEGCLSFPGVSAKVNRATVVKIKALNEFGEEIEVEKDGFLARCIQHEIDHLNGITFFDHLGSLKRKMI
EKKYKKLMQENAKS

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=170, Percent_Identity=42.3529411764706, Blast_Score=129, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24645728, Length=158, Percent_Identity=36.0759493670886, Blast_Score=80, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24645726, Length=156, Percent_Identity=30.7692307692308, Blast_Score=77, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19824; Mature: 19824

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMSLEILKYPHPVLKEVAKEVTKDEINDDLRATIAEMHELMLEANGVGLAAIQVGIKKR
CCCCHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHE
FFIMYDNLEEQNPEIITIINPEIIEQNGKIIDEEGCLSFPGVSAKVNRATVVKIKALNEF
EEEEECCCCCCCCCEEEEECHHHHHCCCCEECCCCCCCCCCCCCCCCCEEEEEEHHHHHH
GEEIEVEKDGFLARCIQHEIDHLNGITFFDHLGSLKRKMIEKKYKKLMQENAKS
HHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNMSLEILKYPHPVLKEVAKEVTKDEINDDLRATIAEMHELMLEANGVGLAAIQVGIKKR
CCCCHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHE
FFIMYDNLEEQNPEIITIINPEIIEQNGKIIDEEGCLSFPGVSAKVNRATVVKIKALNEF
EEEEECCCCCCCCCEEEEECHHHHHCCCCEECCCCCCCCCCCCCCCCCEEEEEEHHHHHH
GEEIEVEKDGFLARCIQHEIDHLNGITFFDHLGSLKRKMIEKKYKKLMQENAKS
HHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA