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The map label for this gene is lepA [H]

Identifier: 134302734

GI number: 134302734

Start: 1817932

End: 1819716

Strand: Direct

Name: lepA [H]

Synonym: FTW_1939

Alternate gene names: 134302734

Gene position: 1817932-1819716 (Clockwise)

Preceding gene: 134302732

Following gene: 134302735

Centisome position: 95.76

GC content: 37.7

Gene sequence:

>1785_bases
ATGAAAAATATCAGAAACTTTTCGATAATTGCGCATATTGACCATGGTAAGTCTACTCTTTCAGACAGGTTTATACAAGT
CTGTAATGGTCTAAGTGAGCGTGAAATGAAAGAGCAGGTTCTTGATTCTATGGATATAGAAAGAGAGCGTGGGATTACGA
TTAAGGCGCAATCTGTGACGCTTGACTATACAGCTAGAGATGGACAAACATATCAGCTTAATTTCATTGATACACCAGGA
CACGTTGATTTCTCTTATGAGGTGTCGCGCTCATTAGCAGCTTGTGAAGGAGCTTTACTTGTAGTAGATGCCGCTCAAGG
CGTAGAAGCCCAGACAGTAGCAAACTGTTATACTGCCATTGAGCAGAATTTAGAGGTTATACCTATATTAAATAAAATTG
ATTTACCTTCTGCAGAGCCGGATAGAGTAGCTCAAGAAATTGAAAAAATAATCGGTATTGATGCTACAGGGGCTACTACA
TGCAGCGCTAAGATAGGTATTGGTGTAGAAGATGTTTTAGAAACTATTGTTGCTAAAGTTCCAGCTCCTGAAGGTGATGT
TAATGCAAAGCTGCAAGCTTTGATTATAGATTCATGGTTTGATAACTATCTTGGCGTGGTTTCACTTGTCAGAGTCAAAA
ATGGTACCATAAAGAAAGGTGAAAAGTTCAAGGTTATGTCAACAGGAGTTGCTTACCAGGTTGATAGACTCGGAGTATTT
ACGCCGAAAATGAAAGACCTAGATCATCTCAAAGCAGGAGAGGTCGGTTTTATCGTTGCTGGGATTAAGGATATTCATGG
TGCACCAGTTGGTGATACGCTCACTCATGCACATAACCCAACAGATAAGCCTGTACCTGGATTTAAAAAGGTTCAACCTC
AAGTTTATGCAGGGATGTTTACTATAAGTTCTGACGATTATCCTGATTTTAGAGAAGCTCTCGAAAAGTTAAGTTTAAAT
GATGCGTCACTATTTTTTGAGCCAGAGGTATCACAAGCTTTAGGTTTTGGCTTTAGATGTGGTTTCTTGGGTATGCTACA
CATGGAGATTATTCAAGAGAGATTAGAAAGAGAGTATAACCTTGATTTAATCACTTCTGCACCAACAGTTGTTTATAAAG
CTATTAAGAAAGATGGTGAAATTATCGAAGTTGATAATCTATCTAAACTACCAGAGCCAGGAGCGATAGCTGAGATACAA
GAGCCAATTGTAAGGGCAAATATTCTTGTGCCAAAAGATTATGTTGGTAGTGTAATTACTATCTGTATTGAAAAAAGAGG
TGTACAGGTTGATCTTAATTATGTGGGGAATCAAGTTTCTATAACTTATGACCTACCAATGATTGAAGTTGTGTCTGATT
TCTTTGATACTCTTAAATCTGTAACGAAAGGTTATGGATCATTAGATTATGAGTTGATTCGTTATGAGCCAGCGAATATG
GTGTGTCTAGATGTGCTCATAAATGGCGATAAGGTTGATGCATTAGCAAGTATTGTGCATAAAGATCAGGCTAAATACAA
AGGTAGAGAACTAGTTGAGCGTCTCAAAGAGTTGATTCCTAGACAAATGTTTGAGGTCGCAATTCAAGCAGCTATAGGTG
GAACTATCGTTGCAAGAAGTACAGTTAAAGCATTGCGTAAGAACGTTTTAGCGAAATGCTATGGTGGTGACGTTTCACGT
AAGAAAAAACTATTAGAGAAGCAAAAAGAGGGTAAAAAGAGAATGAAGAATATTGGCTCTGTTGAAATTCCTCAAGAAGC
TTTTTTATCAGTGCTTAAAAAATAA

Upstream 100 bases:

>100_bases
ATAGCAATCACTACATTTTAAACTTATTTTATAGCATAATAGGTATCTAGATTTATTAAATAAAAAACTACTTCTTAGTA
GCAAAAAGAGCTTAAATATT

Downstream 100 bases:

>100_bases
AATTATAGTTATTATGGAAAATAAAAAAGTAATAGTAGGTATCTCAGGAGGTGTGGACTCATCAGTTTCAGCTTTGCTCT
TGAAACAGCAAGGTTATGAT

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 594; Mature: 594

Protein sequence:

>594_residues
MKNIRNFSIIAHIDHGKSTLSDRFIQVCNGLSEREMKEQVLDSMDIERERGITIKAQSVTLDYTARDGQTYQLNFIDTPG
HVDFSYEVSRSLAACEGALLVVDAAQGVEAQTVANCYTAIEQNLEVIPILNKIDLPSAEPDRVAQEIEKIIGIDATGATT
CSAKIGIGVEDVLETIVAKVPAPEGDVNAKLQALIIDSWFDNYLGVVSLVRVKNGTIKKGEKFKVMSTGVAYQVDRLGVF
TPKMKDLDHLKAGEVGFIVAGIKDIHGAPVGDTLTHAHNPTDKPVPGFKKVQPQVYAGMFTISSDDYPDFREALEKLSLN
DASLFFEPEVSQALGFGFRCGFLGMLHMEIIQERLEREYNLDLITSAPTVVYKAIKKDGEIIEVDNLSKLPEPGAIAEIQ
EPIVRANILVPKDYVGSVITICIEKRGVQVDLNYVGNQVSITYDLPMIEVVSDFFDTLKSVTKGYGSLDYELIRYEPANM
VCLDVLINGDKVDALASIVHKDQAKYKGRELVERLKELIPRQMFEVAIQAAIGGTIVARSTVKALRKNVLAKCYGGDVSR
KKKLLEKQKEGKKRMKNIGSVEIPQEAFLSVLKK

Sequences:

>Translated_594_residues
MKNIRNFSIIAHIDHGKSTLSDRFIQVCNGLSEREMKEQVLDSMDIERERGITIKAQSVTLDYTARDGQTYQLNFIDTPG
HVDFSYEVSRSLAACEGALLVVDAAQGVEAQTVANCYTAIEQNLEVIPILNKIDLPSAEPDRVAQEIEKIIGIDATGATT
CSAKIGIGVEDVLETIVAKVPAPEGDVNAKLQALIIDSWFDNYLGVVSLVRVKNGTIKKGEKFKVMSTGVAYQVDRLGVF
TPKMKDLDHLKAGEVGFIVAGIKDIHGAPVGDTLTHAHNPTDKPVPGFKKVQPQVYAGMFTISSDDYPDFREALEKLSLN
DASLFFEPEVSQALGFGFRCGFLGMLHMEIIQERLEREYNLDLITSAPTVVYKAIKKDGEIIEVDNLSKLPEPGAIAEIQ
EPIVRANILVPKDYVGSVITICIEKRGVQVDLNYVGNQVSITYDLPMIEVVSDFFDTLKSVTKGYGSLDYELIRYEPANM
VCLDVLINGDKVDALASIVHKDQAKYKGRELVERLKELIPRQMFEVAIQAAIGGTIVARSTVKALRKNVLAKCYGGDVSR
KKKLLEKQKEGKKRMKNIGSVEIPQEAFLSVLKK
>Mature_594_residues
MKNIRNFSIIAHIDHGKSTLSDRFIQVCNGLSEREMKEQVLDSMDIERERGITIKAQSVTLDYTARDGQTYQLNFIDTPG
HVDFSYEVSRSLAACEGALLVVDAAQGVEAQTVANCYTAIEQNLEVIPILNKIDLPSAEPDRVAQEIEKIIGIDATGATT
CSAKIGIGVEDVLETIVAKVPAPEGDVNAKLQALIIDSWFDNYLGVVSLVRVKNGTIKKGEKFKVMSTGVAYQVDRLGVF
TPKMKDLDHLKAGEVGFIVAGIKDIHGAPVGDTLTHAHNPTDKPVPGFKKVQPQVYAGMFTISSDDYPDFREALEKLSLN
DASLFFEPEVSQALGFGFRCGFLGMLHMEIIQERLEREYNLDLITSAPTVVYKAIKKDGEIIEVDNLSKLPEPGAIAEIQ
EPIVRANILVPKDYVGSVITICIEKRGVQVDLNYVGNQVSITYDLPMIEVVSDFFDTLKSVTKGYGSLDYELIRYEPANM
VCLDVLINGDKVDALASIVHKDQAKYKGRELVERLKELIPRQMFEVAIQAAIGGTIVARSTVKALRKNVLAKCYGGDVSR
KKKLLEKQKEGKKRMKNIGSVEIPQEAFLSVLKK

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=602, Percent_Identity=47.5083056478405, Blast_Score=601, Evalue=1e-172,
Organism=Homo sapiens, GI94966754, Length=133, Percent_Identity=44.3609022556391, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI4503483, Length=146, Percent_Identity=41.0958904109589, Blast_Score=106, Evalue=6e-23,
Organism=Homo sapiens, GI25306283, Length=134, Percent_Identity=44.7761194029851, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI19923640, Length=134, Percent_Identity=44.7761194029851, Blast_Score=102, Evalue=8e-22,
Organism=Homo sapiens, GI25306287, Length=134, Percent_Identity=44.7761194029851, Blast_Score=102, Evalue=9e-22,
Organism=Homo sapiens, GI18390331, Length=180, Percent_Identity=35.5555555555556, Blast_Score=100, Evalue=7e-21,
Organism=Homo sapiens, GI310132016, Length=111, Percent_Identity=44.1441441441441, Blast_Score=93, Evalue=8e-19,
Organism=Homo sapiens, GI310110807, Length=111, Percent_Identity=44.1441441441441, Blast_Score=93, Evalue=8e-19,
Organism=Homo sapiens, GI310123363, Length=111, Percent_Identity=44.1441441441441, Blast_Score=93, Evalue=8e-19,
Organism=Homo sapiens, GI217272894, Length=134, Percent_Identity=38.0597014925373, Blast_Score=86, Evalue=9e-17,
Organism=Homo sapiens, GI217272892, Length=134, Percent_Identity=38.0597014925373, Blast_Score=86, Evalue=9e-17,
Organism=Homo sapiens, GI53729339, Length=248, Percent_Identity=28.2258064516129, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI53729337, Length=248, Percent_Identity=28.2258064516129, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI94966752, Length=97, Percent_Identity=38.1443298969072, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1788922, Length=592, Percent_Identity=72.4662162162162, Blast_Score=879, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=513, Percent_Identity=28.4600389863548, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1789738, Length=178, Percent_Identity=33.1460674157303, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1790835, Length=171, Percent_Identity=30.4093567251462, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1789559, Length=223, Percent_Identity=30.9417040358744, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1790412, Length=279, Percent_Identity=26.5232974910394, Blast_Score=63, Evalue=6e-11,
Organism=Escherichia coli, GI1789737, Length=279, Percent_Identity=26.5232974910394, Blast_Score=63, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17557151, Length=611, Percent_Identity=40.7528641571195, Blast_Score=475, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI17556745, Length=155, Percent_Identity=33.5483870967742, Blast_Score=99, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17533571, Length=146, Percent_Identity=37.6712328767123, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17506493, Length=170, Percent_Identity=34.7058823529412, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71988811, Length=173, Percent_Identity=32.9479768786127, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71988819, Length=173, Percent_Identity=32.9479768786127, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17552882, Length=133, Percent_Identity=35.3383458646617, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI71994658, Length=223, Percent_Identity=27.3542600896861, Blast_Score=69, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17556456, Length=309, Percent_Identity=26.537216828479, Blast_Score=68, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323320, Length=603, Percent_Identity=43.1177446102819, Blast_Score=510, Evalue=1e-145,
Organism=Saccharomyces cerevisiae, GI6323098, Length=180, Percent_Identity=35.5555555555556, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6324707, Length=145, Percent_Identity=40, Blast_Score=107, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6320593, Length=145, Percent_Identity=40, Blast_Score=107, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6322359, Length=115, Percent_Identity=36.5217391304348, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6324166, Length=145, Percent_Identity=38.6206896551724, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324761, Length=282, Percent_Identity=26.241134751773, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI78706572, Length=603, Percent_Identity=43.6152570480929, Blast_Score=535, Evalue=1e-152,
Organism=Drosophila melanogaster, GI24582462, Length=183, Percent_Identity=34.9726775956284, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI28574573, Length=139, Percent_Identity=42.4460431654676, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24585709, Length=162, Percent_Identity=35.8024691358025, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24585711, Length=162, Percent_Identity=35.8024691358025, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24585713, Length=162, Percent_Identity=35.8024691358025, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI221458488, Length=148, Percent_Identity=37.8378378378378, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21357743, Length=135, Percent_Identity=35.5555555555556, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI28572034, Length=244, Percent_Identity=29.9180327868852, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19921738, Length=280, Percent_Identity=27.1428571428571, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 65513; Mature: 65513

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNIRNFSIIAHIDHGKSTLSDRFIQVCNGLSEREMKEQVLDSMDIERERGITIKAQSVT
CCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHCCCEEEEEEEE
LDYTARDGQTYQLNFIDTPGHVDFSYEVSRSLAACEGALLVVDAAQGVEAQTVANCYTAI
EEEEECCCCEEEEEEECCCCCCEEEHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHH
EQNLEVIPILNKIDLPSAEPDRVAQEIEKIIGIDATGATTCSAKIGIGVEDVLETIVAKV
HCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCEECEECCCCHHHHHHHHHHHC
PAPEGDVNAKLQALIIDSWFDNYLGVVSLVRVKNGTIKKGEKFKVMSTGVAYQVDRLGVF
CCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCEEEECCCCCC
TPKMKDLDHLKAGEVGFIVAGIKDIHGAPVGDTLTHAHNPTDKPVPGFKKVQPQVYAGMF
CCCCCCHHHCCCCCCEEEEECHHHHCCCCCCCHHHCCCCCCCCCCCCHHHCCCCCEEEEE
TISSDDYPDFREALEKLSLNDASLFFEPEVSQALGFGFRCGFLGMLHMEIIQERLEREYN
EECCCCCHHHHHHHHHCCCCCCEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
LDLITSAPTVVYKAIKKDGEIIEVDNLSKLPEPGAIAEIQEPIVRANILVPKDYVGSVIT
CEEEECCHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHEEECCHHHHHHHHH
ICIEKRGVQVDLNYVGNQVSITYDLPMIEVVSDFFDTLKSVTKGYGSLDYELIRYEPANM
HHHHCCCCEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCE
VCLDVLINGDKVDALASIVHKDQAKYKGRELVERLKELIPRQMFEVAIQAAIGGTIVARS
EEEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
TVKALRKNVLAKCYGGDVSRKKKLLEKQKEGKKRMKNIGSVEIPQEAFLSVLKK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MKNIRNFSIIAHIDHGKSTLSDRFIQVCNGLSEREMKEQVLDSMDIERERGITIKAQSVT
CCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHCCCEEEEEEEE
LDYTARDGQTYQLNFIDTPGHVDFSYEVSRSLAACEGALLVVDAAQGVEAQTVANCYTAI
EEEEECCCCEEEEEEECCCCCCEEEHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHH
EQNLEVIPILNKIDLPSAEPDRVAQEIEKIIGIDATGATTCSAKIGIGVEDVLETIVAKV
HCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCEECEECCCCHHHHHHHHHHHC
PAPEGDVNAKLQALIIDSWFDNYLGVVSLVRVKNGTIKKGEKFKVMSTGVAYQVDRLGVF
CCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCEEEECCCCCC
TPKMKDLDHLKAGEVGFIVAGIKDIHGAPVGDTLTHAHNPTDKPVPGFKKVQPQVYAGMF
CCCCCCHHHCCCCCCEEEEECHHHHCCCCCCCHHHCCCCCCCCCCCCHHHCCCCCEEEEE
TISSDDYPDFREALEKLSLNDASLFFEPEVSQALGFGFRCGFLGMLHMEIIQERLEREYN
EECCCCCHHHHHHHHHCCCCCCEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
LDLITSAPTVVYKAIKKDGEIIEVDNLSKLPEPGAIAEIQEPIVRANILVPKDYVGSVIT
CEEEECCHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHEEECCHHHHHHHHH
ICIEKRGVQVDLNYVGNQVSITYDLPMIEVVSDFFDTLKSVTKGYGSLDYELIRYEPANM
HHHHCCCCEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCE
VCLDVLINGDKVDALASIVHKDQAKYKGRELVERLKELIPRQMFEVAIQAAIGGTIVARS
EEEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
TVKALRKNVLAKCYGGDVSRKKKLLEKQKEGKKRMKNIGSVEIPQEAFLSVLKK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA