Click here to switch to the map view.

The map label for this gene is pit [H]

Identifier: 134302651

GI number: 134302651

Start: 1723150

End: 1724145

Strand: Reverse

Name: pit [H]

Synonym: FTW_1836

Alternate gene names: 134302651

Gene position: 1724145-1723150 (Counterclockwise)

Preceding gene: 134302652

Following gene: 134302650

Centisome position: 90.82

GC content: 35.74

Gene sequence:

>996_bases
ATGATTTCATCAGTGCTTATAGCCATTATTGTAATAGCATTGTTTTTTGAGTTTACAAATGGTTTTCATGATGCGGCAAA
TGTTGTTGCAACACCTATTGCAACTAAATCACTAACTCCATATCAGGCAATAGCTTTAGCAGCATTTTTTAACTTTTTAG
GTGCATTTTTTGGTACAGCAGTTGCTGCAACAATATCAAAAGGACTTGTAGATACTAATGTGGTTACAGATATTGTTTTG
ATTTCTGCGTTACTTGGAGCGATAAGTTGGAACTTTTTTACTTGGAGCTTTGGGATTCCTTCAAGCTCATCACATGCTTT
GATAGGTTCTTTAGTTGGCGCAGTGATTATCAGCTCAAGCTATCAGAATGTAAGCTATATGACGGTAGTTAATAAAGTTT
TGATTCCAATGGTTACCTCACCTGTTATTGCATTCTTTTTGGCATTGATAATATGTATCGTGCTGCTAAATATTTTTATG
CGATTTTTTAGAGTTAGAACTACAAATAAATACATAAGAGAAATGCAGGTATTATCTACTAGTTTACTGTCTTTCTCTCA
TGGATCTAATGATGCTCAAAAAACAATGTCAATAATAACACTAGCTTTACTAAGTGCAGGACTTGTTAAGACTACTCAAG
TCCCTGATTGGGTGATTATACTTTGTGGAGTTGCTATGGGATTAGGGACTTTATCTGGCGGTAAAAAGATAATTAAAACA
CTAAGTGCTAAGCTATCAAAACTAGAACCTGTCAATGCTGTTTCTGCTGAGTTAAGCTCAGGGATACTAGTTTTAGGGGC
ATCACATATAGGATTACCAGTTAGTACAACTCAGGTTGCATCTGGTTCTATTATGGGAGCTGGATATGCTGACGCTGGCG
TAAATTGGAAAGTCGTCAAGAAAATGGCAATGGCTTGGATTCTGACTATTCCAGCATGTGTATTCGTCACTAGTGCAATA
TATACCGTAATTTATTATATCTTTGGTAGTTTCTAA

Upstream 100 bases:

>100_bases
TATATAAGGCTATAGAAAATGCGATATCTACATGTACATCTGTAGCTGAGTCTATTATGAGATTATATGTTAAAGAAGTT
TAATCAACAGGGTAGATAAG

Downstream 100 bases:

>100_bases
AAAATCTCTGTGATAAAAAACTTCTATTAAAAAACAAAAAGATTATAATTTAGCATATTTAAAGATTACCCATAAAGTTA
ATGCAAACAAAAATAAATAA

Product: phosphate transporter family protein

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MISSVLIAIIVIALFFEFTNGFHDAANVVATPIATKSLTPYQAIALAAFFNFLGAFFGTAVAATISKGLVDTNVVTDIVL
ISALLGAISWNFFTWSFGIPSSSSHALIGSLVGAVIISSSYQNVSYMTVVNKVLIPMVTSPVIAFFLALIICIVLLNIFM
RFFRVRTTNKYIREMQVLSTSLLSFSHGSNDAQKTMSIITLALLSAGLVKTTQVPDWVIILCGVAMGLGTLSGGKKIIKT
LSAKLSKLEPVNAVSAELSSGILVLGASHIGLPVSTTQVASGSIMGAGYADAGVNWKVVKKMAMAWILTIPACVFVTSAI
YTVIYYIFGSF

Sequences:

>Translated_331_residues
MISSVLIAIIVIALFFEFTNGFHDAANVVATPIATKSLTPYQAIALAAFFNFLGAFFGTAVAATISKGLVDTNVVTDIVL
ISALLGAISWNFFTWSFGIPSSSSHALIGSLVGAVIISSSYQNVSYMTVVNKVLIPMVTSPVIAFFLALIICIVLLNIFM
RFFRVRTTNKYIREMQVLSTSLLSFSHGSNDAQKTMSIITLALLSAGLVKTTQVPDWVIILCGVAMGLGTLSGGKKIIKT
LSAKLSKLEPVNAVSAELSSGILVLGASHIGLPVSTTQVASGSIMGAGYADAGVNWKVVKKMAMAWILTIPACVFVTSAI
YTVIYYIFGSF
>Mature_331_residues
MISSVLIAIIVIALFFEFTNGFHDAANVVATPIATKSLTPYQAIALAAFFNFLGAFFGTAVAATISKGLVDTNVVTDIVL
ISALLGAISWNFFTWSFGIPSSSSHALIGSLVGAVIISSSYQNVSYMTVVNKVLIPMVTSPVIAFFLALIICIVLLNIFM
RFFRVRTTNKYIREMQVLSTSLLSFSHGSNDAQKTMSIITLALLSAGLVKTTQVPDWVIILCGVAMGLGTLSGGKKIIKT
LSAKLSKLEPVNAVSAELSSGILVLGASHIGLPVSTTQVASGSIMGAGYADAGVNWKVVKKMAMAWILTIPACVFVTSAI
YTVIYYIFGSF

Specific function: Low-affinity inorganic phosphate transport [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=158, Percent_Identity=29.746835443038, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI5803173, Length=157, Percent_Identity=29.9363057324841, Blast_Score=86, Evalue=4e-17,
Organism=Escherichia coli, GI1789360, Length=217, Percent_Identity=31.3364055299539, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1789907, Length=221, Percent_Identity=32.1266968325792, Blast_Score=99, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI25146401, Length=173, Percent_Identity=27.7456647398844, Blast_Score=81, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17539280, Length=162, Percent_Identity=27.7777777777778, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71981576, Length=167, Percent_Identity=27.5449101796407, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI32566716, Length=163, Percent_Identity=30.6748466257669, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319773, Length=158, Percent_Identity=32.2784810126582, Blast_Score=80, Evalue=6e-16,
Organism=Drosophila melanogaster, GI21356511, Length=183, Percent_Identity=25.1366120218579, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 35078; Mature: 35078

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISSVLIAIIVIALFFEFTNGFHDAANVVATPIATKSLTPYQAIALAAFFNFLGAFFGTA
CHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VAATISKGLVDTNVVTDIVLISALLGAISWNFFTWSFGIPSSSSHALIGSLVGAVIISSS
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHCC
YQNVSYMTVVNKVLIPMVTSPVIAFFLALIICIVLLNIFMRFFRVRTTNKYIREMQVLST
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLLSFSHGSNDAQKTMSIITLALLSAGLVKTTQVPDWVIILCGVAMGLGTLSGGKKIIKT
HHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHH
LSAKLSKLEPVNAVSAELSSGILVLGASHIGLPVSTTQVASGSIMGAGYADAGVNWKVVK
HHHHHHHCCCCHHHHHHHHCCEEEEECCCCCCCCCHHHHCCCCEEECCCCCCCCCHHHHH
KMAMAWILTIPACVFVTSAIYTVIYYIFGSF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]