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The map label for this gene is rpoA2

Identifier: 134302561

GI number: 134302561

Start: 1641307

End: 1642278

Strand: Reverse

Name: rpoA2

Synonym: FTW_1733

Alternate gene names: 134302561

Gene position: 1642278-1641307 (Counterclockwise)

Preceding gene: 134302562

Following gene: 134302560

Centisome position: 86.51

GC content: 33.02

Gene sequence:

>972_bases
GTGAGTAATAATAATTCAAAACTGGAATTTGTACCTAATATACAGCTTAAAGAAGACTTAGGAGCTTTTAGCTATAAAGT
CCAACTTTCTCCTGTAGAAAAAGGTATGGCTCATATCCTTGGTAACTCTATTAGAAGGGTTTTATTATCTTCACTATCAG
GTGCATCTATAATTAAAGTAAACATCGCTAATGTACTACATGAGTATTCTACTTTAGAAGATGTAAAAGAAGATGTTGTT
GAAATTGTTTCTAATTTGAAAAAGGTTGCGATAAAGCTTGATACAGGTATAGATAGACTAGATTTAGAACTATCTGTAAA
TAAATCAGGTGTAGTTAGCGCTGGAGATTTTAAGACGACTCAAGGTGTAGAAATAATAAATAAAGATCAGCCAATAGCTA
CTTTGACAAACCAAAGAGCATTTAGCTTAACTGCTACAGTGAGTGTAGGTAGAAATGTCGGAATACTTTCTGCGATACCA
ACCGAGCTTGAGAGAGTTGGTGATATAGCTGTAGATGCTGATTTTAATCCTATTAAAAGAGTTGCTTTTGAGGTTTTTGA
TAATGGTGATAGTGAAACTTTAGAAGTATTTGTAAAGACAAATGGTACTATAGAACCACTAGCAGCTGTTACGAAAGCTT
TAGAGTATTTCTGTGAGCAAATATCAGTATTTGTATCTCTAAGAGTACCTAGTAATGGTAAAACAGGTGATGTATTAATA
GATTCTAATATTGATCCTATCCTTCTTAAGCCGATTGATGATTTAGAGCTAACTGTCAGATCATCTAACTGTCTGCGTGC
AGAAAACATTAAGTATCTTGGTGATTTGGTACAGTATTCTGAATCACAGCTTATGAAGATACCTAACTTAGGTAAGAAAT
CTCTCAATGAGATCAAACAAATTTTAATAGATAATAACTTGTCTCTAGGTGTCCAAATTGACAATTTTAGAGAGCTAGTT
GAAGGAAAATAA

Upstream 100 bases:

>100_bases
AGACATAAATGAACAATTAATCATCGAGCTTTACTCTAAGTAATAATTTATTATTATTTTTTAGTGACTCGTTTATAGCC
TTATTTCAAGGAGATTTTTA

Downstream 100 bases:

>100_bases
CAGTATAGTAATCATATAATATTAACTATAAAGGAGTAATTACTATGAGACATCGTAAGCAAGGTAGAAAGTTCGGTAGA
ACTAGTAGTCATAGAAAAGC

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha 1; RNA polymerase subunit alpha 1; Transcriptase subunit alpha 1

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MSNNNSKLEFVPNIQLKEDLGAFSYKVQLSPVEKGMAHILGNSIRRVLLSSLSGASIIKVNIANVLHEYSTLEDVKEDVV
EIVSNLKKVAIKLDTGIDRLDLELSVNKSGVVSAGDFKTTQGVEIINKDQPIATLTNQRAFSLTATVSVGRNVGILSAIP
TELERVGDIAVDADFNPIKRVAFEVFDNGDSETLEVFVKTNGTIEPLAAVTKALEYFCEQISVFVSLRVPSNGKTGDVLI
DSNIDPILLKPIDDLELTVRSSNCLRAENIKYLGDLVQYSESQLMKIPNLGKKSLNEIKQILIDNNLSLGVQIDNFRELV
EGK

Sequences:

>Translated_323_residues
MSNNNSKLEFVPNIQLKEDLGAFSYKVQLSPVEKGMAHILGNSIRRVLLSSLSGASIIKVNIANVLHEYSTLEDVKEDVV
EIVSNLKKVAIKLDTGIDRLDLELSVNKSGVVSAGDFKTTQGVEIINKDQPIATLTNQRAFSLTATVSVGRNVGILSAIP
TELERVGDIAVDADFNPIKRVAFEVFDNGDSETLEVFVKTNGTIEPLAAVTKALEYFCEQISVFVSLRVPSNGKTGDVLI
DSNIDPILLKPIDDLELTVRSSNCLRAENIKYLGDLVQYSESQLMKIPNLGKKSLNEIKQILIDNNLSLGVQIDNFRELV
EGK
>Mature_322_residues
SNNNSKLEFVPNIQLKEDLGAFSYKVQLSPVEKGMAHILGNSIRRVLLSSLSGASIIKVNIANVLHEYSTLEDVKEDVVE
IVSNLKKVAIKLDTGIDRLDLELSVNKSGVVSAGDFKTTQGVEIINKDQPIATLTNQRAFSLTATVSVGRNVGILSAIPT
ELERVGDIAVDADFNPIKRVAFEVFDNGDSETLEVFVKTNGTIEPLAAVTKALEYFCEQISVFVSLRVPSNGKTGDVLID
SNIDPILLKPIDDLELTVRSSNCLRAENIKYLGDLVQYSESQLMKIPNLGKKSLNEIKQILIDNNLSLGVQIDNFRELVE
GK

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=326, Percent_Identity=42.0245398773006, Blast_Score=229, Evalue=3e-61,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA1_FRAT1 (Q14J95)

Other databases:

- EMBL:   AM286280
- RefSeq:   YP_666531.1
- ProteinModelPortal:   Q14J95
- STRING:   Q14J95
- GeneID:   4200326
- GenomeReviews:   AM286280_GR
- KEGG:   ftf:FTF0350
- eggNOG:   COG0202
- HOGENOM:   HBG430844
- OMA:   FGTTLGN
- ProtClustDB:   PRK05182
- BioCyc:   FTUL393115:FTF0350-MONOMER
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 35358; Mature: 35227

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNNNSKLEFVPNIQLKEDLGAFSYKVQLSPVEKGMAHILGNSIRRVLLSSLSGASIIKV
CCCCCCEEEEECCCEEHHCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
NIANVLHEYSTLEDVKEDVVEIVSNLKKVAIKLDTGIDRLDLELSVNKSGVVSAGDFKTT
EHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEEEECCCCCEECCCCCCC
QGVEIINKDQPIATLTNQRAFSLTATVSVGRNVGILSAIPTELERVGDIAVDADFNPIKR
CCCEEECCCCCEEEECCCCEEEEEEEEECCCCCCCHHHCHHHHHHHCCEEEECCCCHHHH
VAFEVFDNGDSETLEVFVKTNGTIEPLAAVTKALEYFCEQISVFVSLRVPSNGKTGDVLI
HHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCEEE
DSNIDPILLKPIDDLELTVRSSNCLRAENIKYLGDLVQYSESQLMKIPNLGKKSLNEIKQ
ECCCCEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHH
ILIDNNLSLGVQIDNFRELVEGK
HHHCCCCEEEEEEHHHHHHHCCC
>Mature Secondary Structure 
SNNNSKLEFVPNIQLKEDLGAFSYKVQLSPVEKGMAHILGNSIRRVLLSSLSGASIIKV
CCCCCEEEEECCCEEHHCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
NIANVLHEYSTLEDVKEDVVEIVSNLKKVAIKLDTGIDRLDLELSVNKSGVVSAGDFKTT
EHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEEEECCCCCEECCCCCCC
QGVEIINKDQPIATLTNQRAFSLTATVSVGRNVGILSAIPTELERVGDIAVDADFNPIKR
CCCEEECCCCCEEEECCCCEEEEEEEEECCCCCCCHHHCHHHHHHHCCEEEECCCCHHHH
VAFEVFDNGDSETLEVFVKTNGTIEPLAAVTKALEYFCEQISVFVSLRVPSNGKTGDVLI
HHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCEEE
DSNIDPILLKPIDDLELTVRSSNCLRAENIKYLGDLVQYSESQLMKIPNLGKKSLNEIKQ
ECCCCEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHH
ILIDNNLSLGVQIDNFRELVEGK
HHHCCCCEEEEEEHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA