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The map label for this gene is slt [H]

Identifier: 134302517

GI number: 134302517

Start: 1589370

End: 1591313

Strand: Reverse

Name: slt [H]

Synonym: FTW_1674

Alternate gene names: 134302517

Gene position: 1591313-1589370 (Counterclockwise)

Preceding gene: 134302520

Following gene: 134302516

Centisome position: 83.82

GC content: 31.02

Gene sequence:

>1944_bases
TTGATTTTATCTAGTACTATTGGTTATAGTCTCACGACACAACAAGTTGAATATTCTCAAAAAGCTATAGATGCATTAGC
TAAAAAAGATTATAAATCATATTATTATTATAAATCAAAACTCAAAGATACTAGCATTTATCCATACTTACAATATAAAG
AAATAAGTACAGATCCAGATATTTTTCAGCAAACAACAATAGATGAGTATTTTAAACAAGACAATAACAGCTATTGGCAA
AATCGCCTTAGCGATGATTTAGCGCAATATTATGCTCAAAAACAGGAATGGAAACTCTTTGATAAATATTATAAAGGTGA
CTTGAGTATATCTGGTAAGTGTTGGAGTATGCAAGCAGAATATGAATCAGGTGACAAAAACAAAGCATTAAATGAATATG
GTCAACTTTGGCAGAATCGAGTATATATGCCTGCTGCATGTAATCCAATGCAAAAATATTGGGATAATTCTGACTATAAA
CCAAGCAGCTATCTAACAACTAAAGCATATACTCTTGCTTTTGCTAATAAATTTGATGATAGCCTGTGGCTATTAAATAC
TTATGTTAAAGATAACAAAGATTATCTTAATTATATAACGGCATGGAAACAGGCTACTAAAGATCCACGTAAATTGGATA
GCTTCATTAATAGATTTCATAACTATAATCATTTTGACAAAGTATTTGTTGATATTTCTAGAGACCTAATAAGAAAAGAT
GTTGAAAGCTACGCAAAAGTATGGAATAACCTAAAAAATAAAAGATATCTAAGCACGAAAGTGAAGCAACAAACTATCTC
AGCAATAGCAGTAAGCTTTGCAAGGTCACAATCACCACAAGCAAAACAGTGGCTAAGCAAAGTAGATAAAAATTATCTTG
ATACAACAGCTTGGGAGTGGTTGTTAAGAGTTGATCTATATAATGAGAACTTCAAAGATTATATACAAACATACAATCAG
CTACCTAAAAATTCTCAACAAGACCAAGCATGGAGATATTGGCTAGCATATAGTTATCAACAGACAGGCCAAAAAGCAAA
AGCTGAAGATATATTTGAGAGTTTGACTAAGACTCCTCTTGATTATTATTCATTCTTAGCGGCTGATAAGCTTGGTAAGC
CATATAACTTTGGTAATGATGTTGCTACAGCATTAACTAATAGTGAAACTAAAAAACTACTAACAGAAGATACTACTGTG
CAGGCTATTGACTTATATCAGATCGGTCAATATAAAGATTCAACAAGTATCTGGCAATGGGCAATTAGAAATAAGCTTAG
AGATAAACAGATTGACGATATTAAACAACTTGCAAGATTAGCTGAGGATAAGCAAATGTATTATGCAGCTATATTCAATA
TGTCAGTTATTGGTAGTTATAATAGTATTGACATGCTCTTTCCGAAAGCTTTTATAAATATAGTTAATCAAAATGCTCAA
AAATTTGCTATTGATAAAGATCTTGTTTTATCAATTATGCGTAAAGAATCACTATTTGATATTAGCGCGGGTTCTTCAGC
TGGAGCAAAAGGATTGATGCAGGTTACAGAACCAACTGCAAAATTTATAGCTCAAAAATATAAATTATCTCTAGTTGGTG
ATAACTCTCAAGGTATGACTAGTCAGATATTTATCCCAGAGAATAATATTAAACTTGGAACAGCTAATTTATATTTCCTA
GAGAAGCTTTTTGATAAGAATCCTGTGTTAGGAATAGCAGCTTATAATGCTGGTCCTGGTAATGTTGCTAAGTGGCTAAA
TGAAAATGAGGTTCCAGCAGCAATTTGGATAGAGAATATTCCATTTGGTGAAACACGTCATTATGTAAGAAAAGTTCTAA
TGTATATGATTGTATATAATAATTTTGTGTTTAAAGATAAAAAAGATCATATTAGTAATTTCTTGGGTTATAAGATATCT
GATAAGCAAAGTTTTAGAAAATAA

Upstream 100 bases:

>100_bases
TATTAGTTTTTTAATATTAATAATGTTAGTATTTTTAGTAAAATAATTTTCTAATTCTGAAATCATTGTGATTAATAAAA
AGTTCATAACAAGTTGTTTA

Downstream 100 bases:

>100_bases
TATTCAAATTATTTTAAAAAGAATATTGTAAACCTGATGTGACAAAATTTTGTGAGTTTCCTGAAAATGAAATACTATAT
GTATAAGTTACTTTAAGGCT

Product: lytic murein transglycosylase

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 647; Mature: 647

Protein sequence:

>647_residues
MILSSTIGYSLTTQQVEYSQKAIDALAKKDYKSYYYYKSKLKDTSIYPYLQYKEISTDPDIFQQTTIDEYFKQDNNSYWQ
NRLSDDLAQYYAQKQEWKLFDKYYKGDLSISGKCWSMQAEYESGDKNKALNEYGQLWQNRVYMPAACNPMQKYWDNSDYK
PSSYLTTKAYTLAFANKFDDSLWLLNTYVKDNKDYLNYITAWKQATKDPRKLDSFINRFHNYNHFDKVFVDISRDLIRKD
VESYAKVWNNLKNKRYLSTKVKQQTISAIAVSFARSQSPQAKQWLSKVDKNYLDTTAWEWLLRVDLYNENFKDYIQTYNQ
LPKNSQQDQAWRYWLAYSYQQTGQKAKAEDIFESLTKTPLDYYSFLAADKLGKPYNFGNDVATALTNSETKKLLTEDTTV
QAIDLYQIGQYKDSTSIWQWAIRNKLRDKQIDDIKQLARLAEDKQMYYAAIFNMSVIGSYNSIDMLFPKAFINIVNQNAQ
KFAIDKDLVLSIMRKESLFDISAGSSAGAKGLMQVTEPTAKFIAQKYKLSLVGDNSQGMTSQIFIPENNIKLGTANLYFL
EKLFDKNPVLGIAAYNAGPGNVAKWLNENEVPAAIWIENIPFGETRHYVRKVLMYMIVYNNFVFKDKKDHISNFLGYKIS
DKQSFRK

Sequences:

>Translated_647_residues
MILSSTIGYSLTTQQVEYSQKAIDALAKKDYKSYYYYKSKLKDTSIYPYLQYKEISTDPDIFQQTTIDEYFKQDNNSYWQ
NRLSDDLAQYYAQKQEWKLFDKYYKGDLSISGKCWSMQAEYESGDKNKALNEYGQLWQNRVYMPAACNPMQKYWDNSDYK
PSSYLTTKAYTLAFANKFDDSLWLLNTYVKDNKDYLNYITAWKQATKDPRKLDSFINRFHNYNHFDKVFVDISRDLIRKD
VESYAKVWNNLKNKRYLSTKVKQQTISAIAVSFARSQSPQAKQWLSKVDKNYLDTTAWEWLLRVDLYNENFKDYIQTYNQ
LPKNSQQDQAWRYWLAYSYQQTGQKAKAEDIFESLTKTPLDYYSFLAADKLGKPYNFGNDVATALTNSETKKLLTEDTTV
QAIDLYQIGQYKDSTSIWQWAIRNKLRDKQIDDIKQLARLAEDKQMYYAAIFNMSVIGSYNSIDMLFPKAFINIVNQNAQ
KFAIDKDLVLSIMRKESLFDISAGSSAGAKGLMQVTEPTAKFIAQKYKLSLVGDNSQGMTSQIFIPENNIKLGTANLYFL
EKLFDKNPVLGIAAYNAGPGNVAKWLNENEVPAAIWIENIPFGETRHYVRKVLMYMIVYNNFVFKDKKDHISNFLGYKIS
DKQSFRK
>Mature_647_residues
MILSSTIGYSLTTQQVEYSQKAIDALAKKDYKSYYYYKSKLKDTSIYPYLQYKEISTDPDIFQQTTIDEYFKQDNNSYWQ
NRLSDDLAQYYAQKQEWKLFDKYYKGDLSISGKCWSMQAEYESGDKNKALNEYGQLWQNRVYMPAACNPMQKYWDNSDYK
PSSYLTTKAYTLAFANKFDDSLWLLNTYVKDNKDYLNYITAWKQATKDPRKLDSFINRFHNYNHFDKVFVDISRDLIRKD
VESYAKVWNNLKNKRYLSTKVKQQTISAIAVSFARSQSPQAKQWLSKVDKNYLDTTAWEWLLRVDLYNENFKDYIQTYNQ
LPKNSQQDQAWRYWLAYSYQQTGQKAKAEDIFESLTKTPLDYYSFLAADKLGKPYNFGNDVATALTNSETKKLLTEDTTV
QAIDLYQIGQYKDSTSIWQWAIRNKLRDKQIDDIKQLARLAEDKQMYYAAIFNMSVIGSYNSIDMLFPKAFINIVNQNAQ
KFAIDKDLVLSIMRKESLFDISAGSSAGAKGLMQVTEPTAKFIAQKYKLSLVGDNSQGMTSQIFIPENNIKLGTANLYFL
EKLFDKNPVLGIAAYNAGPGNVAKWLNENEVPAAIWIENIPFGETRHYVRKVLMYMIVYNNFVFKDKKDHISNFLGYKIS
DKQSFRK

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=596, Percent_Identity=24.1610738255034, Blast_Score=145, Evalue=6e-36,
Organism=Escherichia coli, GI87082191, Length=165, Percent_Identity=31.5151515151515, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 75657; Mature: 75657

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILSSTIGYSLTTQQVEYSQKAIDALAKKDYKSYYYYKSKLKDTSIYPYLQYKEISTDPD
CCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHH
IFQQTTIDEYFKQDNNSYWQNRLSDDLAQYYAQKQEWKLFDKYYKGDLSISGKCWSMQAE
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCEEEEECC
YESGDKNKALNEYGQLWQNRVYMPAACNPMQKYWDNSDYKPSSYLTTKAYTLAFANKFDD
CCCCCCHHHHHHHHHHHHCCEECCCCCCHHHHHCCCCCCCCCCCEEHHHHHHHHHHCCCC
SLWLLNTYVKDNKDYLNYITAWKQATKDPRKLDSFINRFHNYNHFDKVFVDISRDLIRKD
CEEEEHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
VESYAKVWNNLKNKRYLSTKVKQQTISAIAVSFARSQSPQAKQWLSKVDKNYLDTTAWEW
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LLRVDLYNENFKDYIQTYNQLPKNSQQDQAWRYWLAYSYQQTGQKAKAEDIFESLTKTPL
HHEEHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCH
DYYSFLAADKLGKPYNFGNDVATALTNSETKKLLTEDTTVQAIDLYQIGQYKDSTSIWQW
HHHHHHHHHHCCCCCCCCCHHHHHHCCHHHHHHHHCCCCEEEEEHHCCCCCCCCHHHHHH
AIRNKLRDKQIDDIKQLARLAEDKQMYYAAIFNMSVIGSYNSIDMLFPKAFINIVNQNAQ
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCH
KFAIDKDLVLSIMRKESLFDISAGSSAGAKGLMQVTEPTAKFIAQKYKLSLVGDNSQGMT
HHCCCHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCCE
SQIFIPENNIKLGTANLYFLEKLFDKNPVLGIAAYNAGPGNVAKWLNENEVPAAIWIENI
EEEEEECCCEEEECHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHCCCCCCCEEEEEECC
PFGETRHYVRKVLMYMIVYNNFVFKDKKDHISNFLGYKISDKQSFRK
CCCHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHCCCCCCHHHCCC
>Mature Secondary Structure
MILSSTIGYSLTTQQVEYSQKAIDALAKKDYKSYYYYKSKLKDTSIYPYLQYKEISTDPD
CCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHH
IFQQTTIDEYFKQDNNSYWQNRLSDDLAQYYAQKQEWKLFDKYYKGDLSISGKCWSMQAE
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCEEEEECC
YESGDKNKALNEYGQLWQNRVYMPAACNPMQKYWDNSDYKPSSYLTTKAYTLAFANKFDD
CCCCCCHHHHHHHHHHHHCCEECCCCCCHHHHHCCCCCCCCCCCEEHHHHHHHHHHCCCC
SLWLLNTYVKDNKDYLNYITAWKQATKDPRKLDSFINRFHNYNHFDKVFVDISRDLIRKD
CEEEEHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
VESYAKVWNNLKNKRYLSTKVKQQTISAIAVSFARSQSPQAKQWLSKVDKNYLDTTAWEW
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LLRVDLYNENFKDYIQTYNQLPKNSQQDQAWRYWLAYSYQQTGQKAKAEDIFESLTKTPL
HHEEHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCH
DYYSFLAADKLGKPYNFGNDVATALTNSETKKLLTEDTTVQAIDLYQIGQYKDSTSIWQW
HHHHHHHHHHCCCCCCCCCHHHHHHCCHHHHHHHHCCCCEEEEEHHCCCCCCCCHHHHHH
AIRNKLRDKQIDDIKQLARLAEDKQMYYAAIFNMSVIGSYNSIDMLFPKAFINIVNQNAQ
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCH
KFAIDKDLVLSIMRKESLFDISAGSSAGAKGLMQVTEPTAKFIAQKYKLSLVGDNSQGMT
HHCCCHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCCE
SQIFIPENNIKLGTANLYFLEKLFDKNPVLGIAAYNAGPGNVAKWLNENEVPAAIWIENI
EEEEEECCCEEEECHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHCCCCCCCEEEEEECC
PFGETRHYVRKVLMYMIVYNNFVFKDKKDHISNFLGYKISDKQSFRK
CCCHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]